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排序方式: 共有3397条查询结果,搜索用时 31 毫秒
91.
Development of angiogenic cell and gene therapy by transplantation of umbilical cord blood with vascular endothelial growth factor gene. 总被引:11,自引:0,他引:11
Yukihiro Ikeda Noboru Fukuda Mika Wada Taro Matsumoto Aya Satomi Shin-Ichiro Yokoyama Satoshi Saito Koichi Matsumoto Katsuo Kanmatsuse Hideo Mugishima 《Hypertension research》2004,27(2):119-128
Endothelial progenitor cells (EPCs) are present in the mononuclear cells (MNCs) of umbilical cord blood and peripheral blood. To establish the efficiency of angiogenic cell and gene therapies, we transfected the human vascular endothelial growth factor (hVEGF) gene into cord blood MNCs to enhance endothelialization. MNCs from cord blood and peripheral blood were isolated and transfected with pCR3 expressing hVEGF165 or GFP by the Hemagglutinating Virus of Japan (HVJ)-envelope and the cells were cultured in endothelium basal medium-2. The number of attached cells from cord blood was higher than that from peripheral blood. Attached cells expressed Flk-1, VE-cadherin, PECAM-1, CD34, and Tie-2. The increase in the number of attached cells was transient with the transfection of vascular endothelial growth factor (VEGF) gene early in the experimental period. Flt-1 mRNA was not expressed early in the culture period, but was expressed at 2 weeks after separation. VEGF gene transfer into MNCs at 12 days after separation, i.e., when Flt-1 mRNA was expressed continuously, increased the number of attached cells. We evaluated the effects of the transplantation of cord blood MNCs expressing the hVEGF gene on regional blood flow in an ischemic area in a rat model of chronic hindlimb ischemia. Blood flow was significantly improved in nude rats that received transplanted control MNCs. Transplantation of cord blood MNCs transfected with the hVEGF gene yielded greater improvements in blood flow. These results indicate that the hVEGF gene enhances endothelialization of EPCs, and that the transplantation of cord blood MNCs transfected with the VEGF gene may be feasible for the treatment of ischemic diseases as a type of angiogenic cell and gene therapy. 相似文献
92.
Masahiro Goto Naoyuki Miyagawa Kaori Kikunaga Masaru Miura Yukihiro Hasegawa 《Clinical Pediatric Endocrinology》2015,24(3):69-75
Combination treatment with intravenous immunoglobulin (IVIG) plus prednisolone is
effective for prevention of cardiovascular complications in children with Kawasaki disease
(KD). However, administration of prednisolone for approximately 20 d in this regimen
causes adrenocortical suppression in a high proportion of treated children. To establish a
simple method to screen for this suppression, we performed a prospective study on 72
children with KD treated with this regimen in our institution from February 2012 to March
2014. By performing ROC analysis of 21 initial patients treated between February and June
2012, a serum cortisol value at 09:00 h of 5 mcg/dL was established as a threshold for
intact adrenocortical function, which is equivalent to a peak serum cortisol value of
higher than 15 mcg/dL in the CRH stimulation test. Then, we applied this screening test to
51 subsequent patients treated between July 2012 and March 2014. Approximately 90% of the
patients with morning serum cortisol values above 5 mcg/dL 2 to 6 mo after the cessation
of initial prednisolone treatment had peak serum cortisol values exceeding 15 mcg/dL,
suggesting the efficacy of this approach. 相似文献
93.
A randomized controlled trial of radiofrequency ablation with ethanol injection for small hepatocellular carcinoma 总被引:35,自引:0,他引:35
Shiina S Teratani T Obi S Sato S Tateishi R Fujishima T Ishikawa T Koike Y Yoshida H Kawabe T Omata M 《Gastroenterology》2005,129(1):122-130
BACKGROUND & AIMS: Percutaneous radiofrequency ablation is a recently introduced treatment for hepatocellular carcinoma, whereas ethanol injection is now a standard therapy. We compared their long-term outcomes. METHODS: Two hundred thirty-two patients with hepatocellular carcinoma who had 3 or fewer lesions, each 3 cm or less in diameter, and liver function of Child-Pugh class A or B were entered onto a randomized controlled trial. The primary end point was survival, and the secondary end points were overall recurrence and local tumor progression. RESULTS: One hundred eighteen patients were assigned to radiofrequency ablation and 114 to ethanol injection. The number of treatment sessions was smaller (2.1 times vs 6.4 times, respectively, P < .0001) and the length of hospitalization was shorter (10.8 days vs 26.1 days, respectively, P < .0001) in radiofrequency ablation than in ethanol injection. Four-year survival rate was 74% (95% CI: 65%-84%) in radiofrequency ablation and 57% (95% CI: 45%-71%) in ethanol injection. Radiofrequency ablation had a 46% smaller risk of death (adjusted relative risk, 0.54 [95% CI: 0.33-0.89], P = .02), a 43% smaller risk of overall recurrence (adjusted relative risk 0.57 [95% CI: 0.41-0.80], P = .0009), and an 88% smaller risk of local tumor progression (relative risk, 0.12 [95% CI: 0.03-0.55], P = .006) than ethanol injection. The incidence of adverse events was not different between the 2 therapies. CONCLUSIONS: Judging from higher survival but similar adverse events, radiofrequency ablation is superior to ethanol injection for small hepatocellular carcinoma. 相似文献
94.
Satoshi Honjo Yoko Murakami Hiroshi Odajima Yuichi Adachi Koichi Yoshida Yukihiro Ohya Akira Akasawa 《Allergology international》2019,68(1):26-32
Background
Atopic dermatitis (AD) and exercise-induced asthma (EIA) are common in asthmatic children, and exercise is the most common trigger other than infection for acute onset asthma attack in children. We examined whether AD is related to exercise-induced wheezing (EIW), some proxy for EIA.Methods
Japanese version of the International Study of Asthma and Allergies in Childhood questionnaires were used. For 12,405 asthmatic school children, AD was defined as itchy rash coming and going for at least 6 months at any time in the last 12 months with affecting places of flexural parts of body, and severity of AD was rated according to frequency of being kept awake at night with the itch as follows: never in the past 12 months, less than one night per week and one or more nights per week.Results
Adjusted for frequency of asthma attack, odds ratios (OR) of children with current AD as compared to those without AD for having EIW were 1.32 (95% confidence interval = 1.15–1.52), 1.35 (1.14–1.68) and 1.10 (0.92–1.31) for primary school, junior high school and high school children, respectively. EIW was more likely observed in accordance with increasing severity of AD in the primary school children with ORs of 1.12, 1.59 and 1.54 (p for trend < 0.01), and in the junior high school ones with ORs of 1.18, 1.31, 2.03 (<0.01), respectively.Conclusions
AD may be possibly related to EIW. Further studies investigating effect of AD treatment on EIW may be required. 相似文献95.
Prognostic value of pretreatment 18F‐fluorodeoxyglucose positron emission tomography/CT volume‐based parameters in patients with oropharyngeal squamous cell carcinoma with known p16 and p53 status
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Akao H Polisecki E Kajinami K Trompet S Robertson M Ford I Jukema JW de Craen AJ Westendorp RG Shepherd J Packard C Buckley BM Schaefer EJ 《Atherosclerosis》2012,220(2):413-417
Our goal was to determine whether genetic variation at genes affecting statin metabolism or targets of statin therapy would influence low density lipoprotein (LDL) cholesterol lowering with pravastatin, baseline heart disease, or cardiac endpoints on trial. We examined associations of single nucleotide polymorphisms (SNPs) at the liver X receptor alpha (LXRA, rs12221497), and the solute carrier organic anion transporter (SLCO1B1, rs4149056 and rs2306283) gene loci with these variables. We studied 5411 participants in PROSPER (PROspective Study of Pravastatin in the Elderly at Risk) (mean age 75.3 years), who had been randomized to pravastatin 40 mg/day or placebo and were followed for a mean of 3.2 years. No relationships between genetic variation at the LXRA gene locus with statin induced LDL lowering response or other parameters were noted. Both the SLCO1B1 rs4149056 (valine for alanine at 174) and the rs2306283 (asparagine for aspartic acid at 130) SNPs affect the amino acid sequence of the SLCO1B1 gene product. No effect of the rs2306283 SNP on any of the variables was noted. However the presence of the rs4149056 SNP was associated with significantly less LDL cholesterol lowering response to pravastatin (wildtype, 71.5% of the population, -37.0%; heterozygotes, 25.8% of the population, -36.0%; and homozygotes, 2.7% of the population, -31.8%, p=0.003 at 6 months, and p=0.022 at 12 months). Our data indicate that the presence of the rs4149056 non-synonymous SNP at the SLCO1B1 gene locus can significantly decrease the pravastatin induced LDL cholesterol lowering response. 相似文献