铁蛋白对卵巢癌细胞生长和细胞周期动力学的影响

检测Ｈ铁蛋白及Ｌ铁蛋白对人上皮性卵巢癌细胞株ＡＯ及３ＡＯ的生长情况及细胞周期分布的影响。采用３Ｈ标记胸腺嘧啶校苷摄入细胞ＤＮＡ的放射测定法及流式细胞仪进行测定。结果：Ｈ及Ｌ铁蛋白均能抑制 ＡＯ及 ３ＡＯ细胞的生长，此作用在加入铁蛋白后的 ７２ ｈ较 ３６ ｈ显著，且随铁蛋白浓度的增加而增强０．０１＜Ｐ＜０．０５）。在７２时Ｈ铁蛋白的抑制作用较强，有显著差异（Ｐ＜０．０１）。同时发现，加入铁蛋白可使Ｇ１期细胞百分比增加，而Ｓ期细胞明显减少，也有显著差异（Ｐ＜０．０５）。结论：初步认为铁蛋白，特别是Ｈ铁蛋白可抑制卵巢癌细胞生长，并阻止其从 Ｇ０／ Ｇ１；期进入 Ｓ期。     

中药狼疮方对狼疮样BXSB小鼠脾细胞CD134/CD134L表达的影响

目的:探讨CD134/CD134L共刺激信号在SLE发病机制中的可能作用及狼疮方对其表达的影响.方法:采用狼疮样BXSB小鼠模型,随机分为3组:狼疮方治疗组、泼尼松治疗组、空白模型组,每组6只,疗程10周.另设与BXSB小鼠同基因的正常C57BL/6小鼠6只为正常对照组.分别采集上述各组小鼠外周血和脾组织进行检测.结果:(1)与正常对照组比较,模型组BXSB小鼠的血清IgG和抗ds-DNA抗体水平和脾组织CD4 CD134 、CD8 CD134 、CD19 CD134L 、CD23 CD134L 的百分比及CD134、CD134L的mRNA拷贝数都显著增高(P＜0.05或P＜0.01).(2)狼疮方治疗组或泼尼松治疗组的血清IgG、抗ds-DNA抗体水平和脾组织CD4 CD134 、CD8 CD134 、CD19 CD134L 、CD23 CD134L 的百分比及CD134、CD134L的mRNA拷贝数都显著低于模型组(P＜0.05或P＜0.01),而与正常对照组比较无统计学差异(P均＞0.05).结论:狼疮样BXSB小鼠存在CD134/CD134L的异常表达.狼疮方可减轻狼疮样小鼠高丙种球蛋白血症,减少体内自身抗体产生,对CD134/CD134L存在泼尼松相当的下调作用,抑制T、B细胞的异常活化,有效地治疗SLE.     

Down-regulation of the progesterone receptor by the methylation of progesterone receptor gene in endometrial cancer cells

Progesterone plays an important role in the regulation of normal endometrium function by binding to progesterone receptor (PR). In endometrial cancer, however, PR is always down-regulated. Previous reports showed that methylation in the promoter region of the PR gene may be responsible for PRB isoform repression. However, the CpG islands in the exon region of the PR gene are much richer and longer than in the promoter region. We hypothesize that methylation in the exon region may also take part in the down-regulation of the PR gene. The methylation status of the first exon of the PR gene in endometrial cell cultures was investigated. Aberrant methylation patterns were observed in the first exon of PR gene, and the methylation density is correlated with the differentiation of different types of endometrial cancer cells. DNA methyltransferase (DNMT) and histone deacetylase inhibitor 5-aza-2'-deoxycytidine (ADC), as well as trichostatin A (TSA), which reverses PR gene expression, were also studied. A combination of ADC and TSA resulted in synergistic effects in inducing PR expression, down-regulation of DNMT1 and DNMT3A, and could also have antigrowth effect on endometrial cancer cells by inducing apoptosis.     

Polymorphisms in promoter regions of PDHB, SORBS1, and EDG1 genes showing marbling-associated expression changes

We have recently showed that the pyruvate dehydrogenase (lipoamide) beta (PDHB) gene involved in fatty acid oxidation, the sorbin and SH3 domain containing 1 (SORBS1) gene involved in insulin-stimulated glucose uptake, and the endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 (EDG1) gene involved in blood vessel formation possess expression differences in musculus longissimus muscle between low-marbled and high-marbled steer groups. In the present study, we detected single nucleotide polymorphisms (SNPs) in the promoter regions of the 3 genes between the 2 steer groups. A SNP in the EDG1 exhibited significantly different allelic distribution between animals with extremely high predicted breeding value for marbling and with extremely low one. The EDG1 SNP may be related to changes in gene expression and/or marbling.     

Luteinizing hormone upregulates survivin and inhibits apoptosis in ovarian epithelial tumors

Objective

Luteinizing hormone (LH) plays an important role in the development of ovarian cancer, and has been shown to inhibit apoptosis in ovarian cancer cells. Similarly, survivin is a molecule that has been shown to inhibit apoptosis in other types of cancer. Therefore, the aim of this study was to determine whether survivin can be induced by LH in ovarian cancer, and whether this induction influences the sensitivity of ovarian cancers to chemotherapy.

Study design

Survivin expression was monitored using western blot assays, and flow cytometry was used to detect the effects of cisplatin on the induction of apoptosis by LH. MTT assays were also used to analyze rates of cell proliferation.

Results

Administration of LH in vitro induced survivin expression in a dose-dependent manner. Moreover, this signaling was dependent on the ERK1/2 signaling pathway. LH also blocked apoptosis induced by cisplatin.

Conclusion

These results suggest that LH influences the sensitivity of ovarian cancer cells to chemotherapy via signaling to inhibit apoptosis that also upregulates survivin.     

Actinoplanes rishiriensis sp. nov., a novel motile actinomycete isolated by rehydration and centrifugation method

The motile actinomycete strain RI50-RCA114(T) was isolated using rehydration and centrifugation method from a soil sample obtained from Rishiri Island in Japan. The taxonomic status of this organism was established using a polyphasic approach. Cells of strain RI50-RCA114(T) were Gram positive, aerobic, motile and formed irregular sporangia. The strain grew in the presence of 0-2% (w/v) NaCl, between pH 5 and 8, and over a temperature range of 20-37°C, with optimal growth at 30°C. Whole-cell hydrolysates of the strain contained meso-diaminopimelic acid, galactose, glucose and mannose, in addition to one unidentified O-methyl-hexose. The predominant menaquinone was MK-9(H(4)), and the major polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol and phosphatidyl-N-methylethylethanolamine. The major cellular fatty acids were iso-C(16:0), iso-C(15:0) and anteiso-C(17:0). Comparative 16S ribosomal RNA gene sequence analysis revealed that strain RI50-RCA114(T) had the closest sequence similarity with Actinoplanes globisporus JCM 3186(T) (97.6%). However, DNA-DNA hybridization assays as well as physiological and biochemical analyses differentiated strain RI50-RCA114(T) from its closest phylogenetic relative. On the basis of these data, we propose that strain RI50-RCA114(T) (=NBRC 108556(T)=BCC 49184(T)) be classified as the type strain of a novel Actinoplanes species and named Actinoplanes rishiriensis sp. nov.     

阿霉素心肌病大鼠心钠素与丙二醛和超氧化物歧化酶及心室重构的关系

目的探讨阿霉素心肌病(ADR-DCM)大鼠心钠素(ANP)与丙二醛(MDA)、超氧化物歧化酶(SOD)及心室重构的关系。方法以腹腔注射盐酸阿霉素的方法建立大鼠ADR-DCM模型,另设正常对照组;用放射免疫法测定血浆及心肌(心房)匀浆上清液中ANP含量,测定左室质量指数(LVMI),经颈动脉测量左室收缩压(LVSP)、左室舒张末压(LVEDP)、左室压力最大上升及下降速度(±LVdp/dtmax)等心功能指标,测定心肌(左室)匀浆上清液中MDA、SOD含量;病理学切片检测,HE染色,显微镜观察。结果 ADR-DCM组大鼠血浆及心肌(心房)ANP含量显著升高(P<0.01),LVMI显著增加(P<0.01),LVSP、±LVdp/dtmax下降(P<0.01),LVEDP升高(P<0.01),心肌(心房)MDA含量升高(P<0.01),SOD含量降低(P<0.01)。ADR-DCM大鼠血浆ANP含量与±LVdp/dtmax呈负相关(r=-0.78,P<0.05);与LVMI呈正相关(r=0.80,P<0.01)。结论阿霉素通过氧化应激机制损伤心肌,从而导致心室重构及心功能不全,ANP能够反映其中氧化应激及心室重构的程度。     

Cholesteryl group- and acryloyl group-bearing pullulan nanogel to deliver BMP2 and FGF18 for bone tissue engineering

To create a drug delivery system that allows the controlled release of proteins, such as growth factors, over a long-term period, cholesteryl group- and acryloyl group-bearing pullulan (CHPOA) nanogels were aggregated to form fast-degradable hydrogels (CHPOA/hydrogels) by cross-linking with thiol-bearing polyethylene glycol. The gold standard of clinical bone reconstruction therapy with a physiologically active material is treatment with recombinant human bone morphogenetic protein 2 (BMP2); however, this approach has limitations, such as inflammation, poor cost-efficiency, and varying interindividual susceptibility. In this study, two distinct growth factors, BMP2 and recombinant human fibroblast growth factor 18 (FGF18), were applied to a critical-size skull bone defect for bone repair by the CHPOA/hydrogel system. The CHPOA-FGF18/hydrogel displayed identical results to the control CHPOA-PBS/hydrogel, and the CHPOA-BMP2/hydrogel treatment imperfectly induced bone repair. By contrast, the CHPOA-FGF18?+?BMP2/hydrogel treatment strongly enhanced and stabilized the BMP2-dependent bone repair, inducing osteoprogenitor cell infiltration inside and around the hydrogel. This report indicates that the CHPOA/hydrogel system can successfully deliver two different proteins to the bone defect to induce effective bone repair. The combination of the CHPOA/hydrogel system with the growth factors FGF18 and BMP2 might be a step towards efficient bone tissue engineering.