鱼金清解口服液质量标准研究

目的建立鱼金清解口服液的质量控制标准。方法采用TLC法对制剂中槲皮苷进行定性鉴别;采用GC法对制剂中芳樟醇进行定性鉴别;采用HPLC法对制剂中绿原酸进行定量测定;采用GC法对制剂中甲基正壬酮进行定量测定。结果 TLC法能检出鱼腥草中的槲皮苷;GC法能检出金银花中的芳樟醇;绿原酸在0.104～2.608μg范围内呈良好的线性关系,r=1.0000,平均回收率为100.3%,RSD=1.37%;甲基正壬酮在1.7932～71.728μg.ml-1范围内呈良好的线性关系,r=1.0000,平均回收率为102.4%,RSD为1.7%。结论本课题所建立的质量标准方法稳定、准确、重复性好,可用于该制剂的质量控制。     

Genomic Analysis Reveals Distinct Subtypes in Two Rare Cases of Primary Ovarian Lymphoma

Primary (localized) non-Hodgkin lymphoma (NHL) of the ovary is extremely rare; only a few cases have been reported in the literature. We report two cases of primary ovarian lymphoma (POL), one involving bilateral ovaries in a 15-year-old girl and other involving one ovary in a 5-year-old girl. This report describes detailed clinical, histopathological, and imaging findings, along with the review of literature of primary diffuse large B-cell lymphoma (DLBCL) arising from an ovary. In addition, we describe findings of targeted capture panel sequencing on both tumors and identify the major genetic mutations that are recurrently mutated in pan-cancers. Compared to the genomic mutation features of major subtypes of DLBCL, we distinguish that each POL belongs to distinctive subtypes, GCB (germinal center B-cell subtype) DLBCL and ABC (activated B-cell subtype) DLBCL, respectively. The findings from the genomic analysis may help to understand the pathogenesis of POL and to guide potential targeted therapy in the future.     

Severe acute pancreatitis is associated with upregulation of the ACE2-angiotensin-(1-7)-Mas axis and promotes increased circulating angiotensin-(1-7)

Background/objectivesAngiotensin-converting enzyme 2 (ACE2), its product angiotensin-(1-7) and its receptor Mas may counteract the adverse effects of the ACE-angiotensin receptor II-AT₁ axis in many diseases. We examined the expression of these novel components of the rennin-angiotensin system in an experimental mouse model of severe acute pancreatitis (SAP).MethodsSAP was induced by six intraperitoneal injections of caerulein, and mice were sacrificed at 2, 12, 24, 48 and 72 h post disease-induction (normal control group mice were sacrificed at 2 h post disease-induction). Tissue and blood were collected for biochemical detection, gene and protein expression by qRT-PCR and western blot analysis, enzyme-linked immunosorbent assay and immunohistology detection.ResultsPancreatic ACE2 gene and protein expression, plasma and pancreatic angiotensin-(1-7) levels and Mas receptor gene and protein expression were significantly increased (p < 0.05) following SAP induction compared with the normal control group.ConclusionsSevere acute pancreatitis is associated with upregulation of the ACE2-angiotensin-(1-7)-Mas axis and promotes increased circulating angiotensin-(1-7). These results support the presence of an ACE2-angiotensin-(1-7)-Mas axis in pancreatitis.     

Three proven cases of insomnia treated by Professor JIA Chunsheng

In this paper, professor JIA Chunsheng's multiple treatment thoughts and methods for insomnia under different pathogenesis were shown through the three proven cases of insomnia, including the type of constraint fire of the liver and gallbladder, and excessive yang harassing the spirit, the type of failure of the heart and kidney to interact declined to kidney yin deficiency and the type of liver constraint transforming into fire, insufficient of the spleen and stomach, which provided physicians with experience and methods during treatment of insomnia in clinic, thus improving the clinical effect.     

81例单纯性先天性心脏病患者NKx2.5基因突变筛查及关联研究

目的:探讨NKx 2.5基因突变和单核苷酸多态性(SNP)与人类单纯性先天性心脏病(CHD)的关系.方法:应用聚合酶链反应结合脱氧核糖核酸(DNA)测序技术,对81例单纯性CHD患者(包括室间隔缺损组37 例,房间隔缺损组17例,圆锥动脉干畸形组18例,动脉导管未闭组5例,主动脉瓣二叶畸形组4例),74例患者一级亲属(一级亲属组)以及52例正常非CHD对照者(正常对照组)的NKx 2.5基因的所有外显子及其侧翼序列进行突变检测,并对SNP位点进行基因分型,分析单个位点的基因型和等位基因频率是否与CHD相关.结果:所有CHD患者、患者一级亲属基因编码均未发现突变,而在NKx 2.5基因外显子1区域内发现1个SNP位点,这个SNP位点位于上游63位碱基63A>G,导致第21位密码子由GAA转变为GAG,碱基替换后仍编码谷氨酸(Glu),属于同义突变 (Glu21Glu).基因型频率和等位基因频率的分布,在房间隔缺损组和正常对照组之间差异均有统计学意义(P均<0.05).结论:NKx 2.5基因突变与我国单纯性CHD之间无明显相关,该基因上的63A>G SNP位点与先天性房间隔缺损之间可能存在相关性.     

Gluconic acid aqueous solution: A bio-based catalytic medium for organic synthesis

Gluconic acid is non-toxic, non-volatile, non-corrosive, and easily biodegradable chemical, which can be produced from biomass using several methods: oxidation by molecular oxygen using heterogeneous catalysis, enzymatic oxidation, and microbial fermentation. Being highly soluble in water, gluconic acid can form concentrated aqueous solutions, and its 50% aqueous solution has found application as a catalytic medium for organic synthesis. For some reactions, gluconic acid aqueous solutions (GAAS) outperform conventional and green solvents when used as media for organic synthesis. GAAS was found to be particularly efficient in the Knoevenagel condensation, the Michael addition, and multicomponent reactions based on them. These and other chemical transformations utilizing GAAS as a medium are discussed in this review. We also compare the effectiveness of GAAS as a new green catalytic medium and the performance of other solvents and catalysts.     

Considerations for Use of Acupuncture as Supplemental Therapy for Patients with Allergic Asthma

This study examines the clinical and immunomodulatory effects of acupuncture in the treatment of patients with allergic asthma. The acupuncture points GV14, BL12, and BL13 were selected based on the theory of traditional Chinese medicine in treating asthma. Manual acupuncture was performed once every other day (three times per week) for 5 weeks. The needles were twisted approximately 360° evenly at the rate of 60 times/min for 20 s, manipulated every 10 min and withdrawn after 30 min. Concentrations of sIgA and total IgA in secretions were determined by the combination of sucrose density gradient ultracentrifugation and RIA. Levels of cortisol in the plasma were measured by RIA. Total IgE in the sera was examined by ELISA. Flow cytometry was used to detect the numbers of CD3+, CD4+, CD8+, and IL-2R + T lymphocytes in the peripheral blood. The absolute and differential numbers of eosinophils in peripheral blood were counted with eosin staining. The total efficacy of the acupuncture treatment in patients with allergic asthma at the end of one course of treatment was 85 %. After treatment, the concentrations of sIgA and total IgA in the saliva (P?<?0.01, P?<?0.02) and nasal secretions (P?<?0.02, P?<?0.02) were significantly decreased in patients with allergic asthma. The levels of total IgE in sera (P?<?0.001), the counts of IL-2R + T lymphocytes (P?<?0.001), and the absolute and differential numbers of eosinophils (P?<?0.01, P?<?0.01) in the peripheral blood were also significantly decreased. The numbers of CD3+, CD4+, and CD8+ T lymphocytes in the peripheral blood were significantly increased in the allergic asthmatics treated by acupuncture (P?<?0.001, P?<?0.01, and P?<?0.001, respectively). The concentration of cortisol in the plasma of asthmatic patients did not change significantly after the acupuncture treatment (P?>?0.05). Acupuncture has regulatory effects on mucosal and cellular immunity in patients with allergic asthma and may be an adjunctive therapy for allergic asthma.