钩体基因疫苗对豚鼠延髓原癌基因表达的影响

我们的研究已表明，赖型０１７株钩体外膜疏水蛋白ＯｍｐＬ３９是稳定的免疫原，在此我们分别用ＯｍｐＬ３９与钩体死菌苗和生理盐水对照免疫豚鼠，研究ＯｍｐＬ３９的免疫保护作用和免疫机理，对ＯｍｐＬ３９的抗原特异性刺激引起中枢原癌基因（Ｃｆｏｓ基因）表达进行了观察。结果表明，ＯｍｐＬ３９在豚鼠体内能产生高效价的阳性抗体，免疫保护率为１００％。在ＯｍｐＬ３９对中枢Ｃｆｏｓ基因表达的影响中，我们观察到ＯｍｐＬ３９免疫的豚鼠较空白和死菌苗组Ｃｆｏｓ表达明显少于死菌苗组和空白对照组（ｐ＜００５）。提示ＯｍｐＬ３９能特异性地抑制Ｃｆｏｓ基因的表达，减轻强毒钩体对动物体内的病理损伤，有较死菌苗强的免疫保护作用。     

中学生自我差异和心理健康的关系

目的 分析中学生自我差异与心理健康的关系。方法 采用自行修订的自我差异量表和SCL—90对500名中学生进行调查。结果 初中生现实自我与理想自我的差异（AI）和现实自我与应该自我的差异（AO）这两类自我差异分数都显著高于高中生。中学生两类自我差异各因子与心理健康各因子之间都显著相关（P〈0.05）。AI道德自我差异对初中生的心理健康有预测作用;AI心理自我差异与AI自我认同差异对高中生的心理健康有预测作用。结论 应针对中学生的心理特点,提高其对自我的认识,减少诱发心理问题的因素,促进中学生心理健康。     

Direct inhibition of rat granulosa cell inhibin production by epidermal growth factor in vitro

The effect of epidermal growth factor (EGF) on inhibin production by rat granulosa cells has been investigated using a recently developed inhibin radioimmunoassay (RIA). Granulosa cells from intact immature diethylstilbestrol (DES)-treated rats were exposed to EGF (1-100 ng/ml) in the presence or absence of FSH for varying periods in vitro. An inhibitory effect of EGF on basal inhibin secretion was evident at day 2 of culture and was sustained over the subsequent 2 days. This action on basal inhibin secretion was dose-dependent, and maximal inhibition to 50% of control was observed at a dose of 100 ng EGF/ml at day 4. EGF also inhibited basal progesterone secretion in a similar manner. EGF caused a dose-dependent inhibition of FSH-stimulated inhibin secretion, with an ID50 (0.5 ng/ml, 0.08 nM) about one-eighth that in the absence of FSH. In addition, EGF also inhibited the stimulation of inhibin production by 8-Br-cAMP and prostaglandin E2. To exclude the possibility that EGF was toxic to the granulosa cells, several biochemical parameters related to cell growth were measured. EGF treatment did not alter cell number but slightly increased [3H]thymidine incorporation into cellular DNA. The effect of EGF on [35S]methionine incorporation into cellular protein was biphasic, being stimulatory at doses less than 10 ng/ml but inhibitory at 100 ng/ml. The present data have demonstrated a direct inhibitory effect of EGF on basal and FSH-stimulated inhibin production by granulosa cells suggesting an important regulatory role of this growth factor in the differentiation of ovarian function.     

深圳宝安3～7岁健康幼儿血清糖、离子、非蛋白含氮类化合物表达水平观察

目的观察深圳宝安3～7岁健康幼儿血清糖、离子、非蛋白含氮类化合物表达水平。方法采用OLRPUSAU-640全自动生化仪及OLRPUS诊断试剂、东欧生物诊断试荆，检测316例体检健康幼儿血清中Ca^＋＋、Mg^＋＋、P^＋＋＋、GLU、BUN、Cr、UA的含量。结果①．1组与4组及2组与3、4组Ca^＋＋组间方差分析，差异有统计学意义，P〈0.05。②．1组与2、3、4组Cr组间方差分析，差异有统计学意义P〈0．05。而Mg^＋＋、P^＋＋＋、GLU、BUN、UA组间方差分析差异无统计学意义，P〉0．05。结论笔者证实了3～4岁健康幼儿与6～7岁健康幼儿及4～5岁健康幼儿与5～7岁健康幼儿血清Ca^＋＋表达水平有差异。也证实了3～4岁健康幼儿与4～7健康幼儿Cr含量存在差异。由此可见，3～7岁健康幼儿建立自己的参考值是必要的。     

BTA在膀胱癌诊断中的应用

为了探求对膀胱肿瘤诊断及术后复发监测的理想手段，从１９９５年９月～１９９６年６月，采用ＢＴＡ试剂检测方法对２０２例血尿患者及７１例膀胱肿瘤术后的患者进行了诊断检测，诊断膀胱癌的敏感度为９６％、特异度为８３％、准确度为８６％，２６例膀胱肿瘤患者中仅６例（２３％）尿脱落细胞阳性，正常对照均为阴性。认为ＢＴＡ试剂是一种快速、有效、无创性诊断膀胱肿瘤的方法。     

Clinico pathologic analysis of 1503 thyroid tumors]

A clinicopathologic analysis of 1503 thyroid tumors was conducted revealing 82.9% of benign and 17.8% of malignant lesions. The most common type of benign tumor was simple adenoma (62.1%) and of malignant tumor, papillary adenocarcinoma (52.5%). The malignant to benign tumor ratio was 1:4.8. The male to female sex ratio was 1:2.6 in the malignant and 1:3.8 in the benign tumors. The overall 5- and 10-year survival rates for all cancers were 67.7% and 47.6%, respectively. It is suggested that the follicular carcinoma could further be divided into well differentiated (papillary and follicular), poorly differentiated (including squamous cell carcinoma) and undifferentiated types.     

新政周岁:新乡医改连环扣

一年前。意图以医改试验牵动下一轮国企改制的新乡市政府，以及梦想着完善大生命产业链最后一环的华源集团。在河南新乡联手导演了一出轰轰烈烈的五家医院整体“捆绑改制”。本刊当时曾以“闪击战”形容那场医改风暴的强劲势头。如今当潮头已过，逐浪而来的是浊流击岸，还是一碧万顷?     

从"集体哗变"看文化再造

去年春节,陈振强这个名字突然出现在公众的视野里,并频现报端。此前,提到南京市第一医院,人们只知道它的心脏手术十分著名,作为该院心外科“二陈”之一的陈振强则鲜为人知。陈振强一夜成名的原因是:与自己的手术团队集体“改嫁”到江苏省中医院,也即媒体所述的“南京市第一起医疗专家集体哗变案”,而陈振强正是此案的“策反者”!10个月后,“哗变”事件又在厦门眼科中心上演,这次的主角是大名鼎鼎的“福建眼科第一刀”洪荣照。因为与医院改制后的新东家存在“理念差异”,洪荣照负气带走了78名医疗骨干。