Identification and molecular characterization of a single-stranded circular DNA virus with similarities to Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1

A putative circular single-stranded DNA (ssDNA) virus was recovered from Hypericum japonicum collected in Vietnam. The viral isolate was tentatively named Hypericum japonicum-associated circular DNA virus (HJasCV). HJasCV shares 58.7-65.4% nucleotide sequence identity with Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1) and SsHADV-1-like viruses. Like this group of viruses, the genome of HJasCV (2 200 nt) has two large ORFs, one in the virion-sense and the other in the complementary-sense DNA. The proteins encoded in the virion-sense and complementary-sense ORFs share 39-46 % and 45-67 % amino acid sequence identity with the putative capsid and replication-associated proteins (Reps), respectively, of SsHADV-1 and SsHADV-1-like viruses. The putative Rep of HJasCV contains all of the motifs related to rolling-circle replication. Its 111-bp intergenic region (IR) contains a hairpin structure with a geminivirus-like nonanucleotide sequence, TAATGTTAT, at the apex of the loop. Phylogenetic analysis revealed that HJasCV forms a monophyletic clade with SsHADV-1 and SsHADV-1-like viruses.     

Influence of oxygen exposure on fermentation process and sensory qualities of Sichuan pickle (paocai)

The physicochemical and microbial changes, volatile profile, texture and appearance were investigated in three groups of Sichuan pickles differing in oxygen exposure during a 64 day fermentation process. At the beginning, all the Sichuan pickles displayed similar sensory and fermentation characteristics regarding lactic acid bacteria domination, sugar utilization, and lactate accumulation. Soon afterwards, continued exposure allowed premature pellicle formation and typical aerobic deterioration of Sichuan pickle, accompanied by texture destruction and an odorous stench characterized by arenes and aldehydes on the 32^nd day. Meanwhile, the facultative anaerobes including Lactobacillus (90 ± 2%), Pediococcus (4.7 ± 0.3%) and Lactococcus (1.2 ± 0.8%) were the dominant genera and probably associated with acetic acid production and arene enrichment under oxygen exposure conditions on the 32^nd day. Until the 48^th day, intermittent oxygen exposure resulted in pellicle formation and an unpleasant flavor characterized by ketones, esters and S-methyl thioacetate. In comparison, Sichuan pickle without oxygen exposure always maintained a relatively favorable fragrant scent and texture. Besides Lactobacillus, anaerobic genera were the main bacteria in Sichuan pickle under closed conditions and accounted for 50 ± 9% of the total bacteria on the 32^nd day. The study indicated the microbial and chemical characteristics of Sichuan pickles during practical production and provided a theoretical guidance for the industrial production of Sichuan pickle.

The physicochemical and microbial changes, volatile profile, texture and appearance were investigated in three groups of Sichuan pickles differing in oxygen exposure during a 64 day fermentation process.     

Radiotheranostic Targeting Cancer Stem Cells in Human Colorectal Cancer Xenografts

Molecular Imaging and Biology - The aim of this study was to perform radiotheranostics with radioiodinated monoclonal antibodies (mAbs) for targeting cancer stem cells (CSCs) in human colorectal...     

中药炮制品检验中出现的不合格问题分析与思考

文章分析了目前中药炮制品在检验中出现的不合格方面,探讨质量管理应采取的措施,建立健全中药炮制品质量保证体系,加强质量管理和监督,提高中药炮制品质量标准,确保用药安全与疗效。     

肝癌患者口服甲磺酸仑伐替尼不良反应护理

本个案针对中国医学科学院北京协和医学院肿瘤医院深圳医院2020年2月14日收治的1例肝癌口服靶向药(甲磺酸仑伐替尼)引起肛周瘙痒和多处皮损的病例进行分析.患者带肛周皮损入院,与压疮区别后,判断为肝癌患者口服甲磺酸仑伐替尼所致药物不良反应,对症给予治疗与护理.伤口明显好转后,患者出院.此案为新发现的甲磺酸仑伐替尼说明书上...     

Research on the influence of polar solvents on CsPbBr3 perovskite QDs

All-inorganic CsPbX₃ (X = Cl, Br, I) perovskite quantum dots (QDs) have become a kind of optoelectronic material with huge application prospects due to their excellent physical and optical properties. However, their poor structural stability to the external environment, especially polar solvents, seriously hinder further development in practical applications. Considering whether polar solvents have the same effects on perovskites QDs, few studies have been investigated in this area presently. In order to find out the effect of different polar solvents on all-inorganic perovskite QDs, in this work, we select 12 kinds of polar solvents of methanol, ethanol, isopropanol, 1-butanol, 1-pentanol, 1-octanol, N,N-dimethylformamide (DMF), tetramethylethylenediamine (TMEDA), ethyl acetate, n-butyl acetate, dibutyl phthalate and acetone for a specific analysis. The characterization of their morphology, optical and physicochemical properties shows that different polar solvents have different effects on all-inorganic perovskite QDs, but their effects are regular. Polar solvents act on the ligands preferentially, and the effects can be divided into: reducing the concentration of ligands; substituting ligands partially; completely destroying the surface ligands; polar solvents with the same functional group, as the polarity of the solvent increases, the impact on all-inorganic perovskite QDs is greater. We believe that this discovery has important implications for improving the stability of all-inorganic perovskite QDs.

All-inorganic CsPbX₃ (X = Cl, Br, I) perovskite quantum dots (QDs) have become a kind of optoelectronic material with huge application prospects due to their excellent physical and optical properties.     

垂直型快套附着体义齿的初步应用

目的：介绍垂直型快套附着体义齿的设计原则、制作方法，评价其疗效。方法：为12例牙列缺损成年患者制作12件垂直型快套附着体义齿，每件义齿设计1副或2副快套附着体，经6个月至2年时间的临床观察。结果：全部病例使用垂直型快套附着体义齿后，固位良好、咀嚼功能好、美观舒适。结论：垂直型快厉型附着本固位好，快套内部的弹性结合产生缓冲作用，使基牙的损伤减少。     

射频电流致心率变异性改变的机理探讨

采用心率功率谱对11例房室结折返性心动过速（A组）、9例右侧旁边（B组）和21例左侧旁道（C组）致房室折返性心动过速的患者于射频消融前、消融后1、2、3、5、10d进行连续动态观察，比较低频段功率（LFP）、高频段功率（HFP）和LFP与HFP的比值。结果显示所有患者于术后1dHFP均降低，但A组持续下降时间长于B、C两组，消融能量小于3500J者，LFP于术后第2天才下降，消融能量大于3500J者手术后第1天即出现下降，结果同时显示上述心率变异性（HRV）的改变于术后10d可恢复至术前水平。表明消融后HRV的降低系副交感神经受损的结果，而消融能量大者亦可同时损伤交感神经，导管的机械刺激和电刺激对HRV无影响。     

计算机辅助导航全膝关节置换术的当前概念和进展

全膝关节置换术(TKR)旨在重建胫股和髌股关节的稳定性,缓解疼痛,改善患者生活质量.计算机辅助导航系统的应用能够有效减少常规TKR手术下肢力线重建中的人为错误并改善手术结果.现有证据表明,应用计算机辅助导航系统能够显著减少术后机械轴和冠状位假体位置的异常值.此外,导航系统提供了常规手术无法获得的软组织平衡客观评估.应用...