全植入式药泵行肝血管灌注治疗晚期肝癌

对２４例不能切除的晚期肝癌采用完全植入式药泵行肝动脉和门静脉双灌注化疗。结果：部分缓解（ＰＲ）１５例（６２．５％），稳定（Ｓ）８例（３３．３％），进展（Ｐ）１例。平均生存期为９．１７月，半年生存率为６６．７％，１年为２５％。在ＡＦＰ＞４００μｇ／Ｌ的１０例中，５例下降。     

261例恶性肿瘤患者血液流变学的检测结果

检测261例恶性肿瘤(肺癌、乳腺癌、胃癌、原发性肝癌、恶性淋巴瘤、食管癌及结直肠癌等)患者的血液流变学水平,并与健康人作了比较。检测结果表明,恶性肿瘤患者的血浆粘度,全血低切粘度及血沉均有明显升高(P<0.05和0.01);而血细胞压积(除乳腺癌外)均低于健康人(P<0.01)。纤维蛋白原水平除恶性淋巴瘤及肠癌外均有明显增高(P<0.05和0.01)。肿瘤复发转移患者仅血浆粘度高于无复发转移者(P<0.01)。作者还对原发性肝癌和肝硬化病人、胃癌和慢性胃炎病人的血液流变学各项指标做了比较分析,结果发现某些指标有明显差异,以血浆粘度变化尤为明显。因此认为血浆粘度对良、恶性的肝、胃疾患可能有一定的鉴别诊断意义。     

青天葵抗甲、乙型流感病毒作用研究

目的研究青天葵在体外抗甲、乙型流感病毒株作用。方法选用狗肾细胞培养法考察青天葵不同极性部位对甲型(FM1)、乙型(昆40B)流感病毒的作用。结果青天葵水溶性部位对甲型流感病毒FM1有抑制作用。结论青天葵具有体外抗甲型流感病毒作用。     

维甲酸对原发性高血压大鼠心肌血管平滑肌增殖的作用及其机制

目的:探讨维甲酸对自发性高血压大鼠(SHR)心肌、血管平滑肌增殖的影响和肾素-血管紧张素-醛固酮系统(RAS)所起的作用。方法:18只SHR大鼠随机分为全反式维甲酸治疗组、对照组、卡托普利治疗组,每组6只。分别予治疗组和对照组皮下注射全反式维甲酸(ATRA)10mg/Kg体重或溶媒,高血压药物治疗组予卡托普利0.25mg/Kg体重管饲,每天一次,均疗程4周。疗程结束后观察各组SHR大鼠心肌、血管平滑肌(VSMC)增殖细胞核抗原(PCNA)的变化以及心肌ACE-mRNA、ACE2-mRNA的表达。结果:维甲酸治疗组心肌、血管平滑肌增殖细胞核抗原(PCNA)表达显著低于对照组(P<0.01),卡托普利治疗组的PCNA表达也显著低于对照组(P<0.01),但维甲酸治疗组与卡托普利组比较也有显著差异(P<0.01);维甲酸治疗组ACE-mRNA的表达低于卡托普利组及对照组(P<0.01),卡托普利组ACE-mRNA的表达也低于对照组(P<0.01);而维甲酸组ACE2-mRNA的表达则高于对照组及卡托普利组(P<0.01),后两组之间则无显著差异(P>0.05)。结论:维甲酸能抑制SHR模型心肌、血管平滑肌PCNA的表达,并在增加ACE2-mRNA表达的同时减少ACE-mRNA的表达,从而有可能干预增殖性血管疾病的病程。     

下腔静脉滤器在治疗下肢深静脉血栓中的应用

目的：探讨下腔静脉滤器在治疗下肢深静脉血栓(DVT)中的应用价值。方法：选择10例DVT患，使用进口铁质滤器，经股静脉或颈静脉途径置人下腔静脉，随访8—20个月。结果：10例患中无一例发生肺动脉栓塞和其他严重并发症。结论：下腔静脉滤器置人方法简单，安全；可有效防止肺动脉栓塞的发生。     

Mass spectrometric analysis of the structure of gamma II bovine lens crystallin.

The amino acid sequence of bovine gamma II-crystallin has been verified by a combination of electrospray and fast atom bombardment mass spectrometry. The molecular weight of gamma II, isolated by gel filtration and ion exchange chromatography, was determined to be 20,967 +/- 3 by electrospray mass spectrometry. Another aliquot of gamma II was completely digested by trypsin in a medium of 20% CH3CN and 0.1 M Tris, pH 8.2. The tryptic peptides were separated by reversed phase HPLC and identified by their molecular weights, as determined by fast atom bombardment mass spectrometry (FABMS). The identification of each peptide was confirmed by digesting the peptide further to give new peptides whose molecular weights were also determined by FABMS and related to the proposed amino acid sequences. The data from both types of mass spectrometric analyses were consistent with the sequence previously proposed by Hay et al. (J. Biol. Chem. 1987, 146, 332-338), including threonine at position 119. The FAB mass spectrum of one HPLC fraction suggested that disulfide bonding between Cys 18 and Cys 22 was present in at least half the protein preparation. Whether the Cys 18/Cys 22 disulfide bond was present in native gamma II or was produced during isolation or enzymic digestion could not be determined from these studies. Samples that had been stored for several weeks showed that several of the cysteines had become disulfide bonded. These studies illustrate the power of mass spectrometric techniques to accurately confirm the primary structure of proteins and to identify post-translational modifications.     

^60Co消毒与高温高压消毒胚胎骨的实验研究

在12只健康家兔下颌骨体部的两侧各造成0.7cm×0.5cm×0.3cm的骨缺损区，分别置入^60Co消毒的胚胎骨和高温高压消毒的胚胎骨，发现高温高压消毒可以替代^60Co消毒胚胎骨进行移植，为临床提供了一种新的用作同种异体移植胚胎骨的消毒方法。     

血清白细胞介素－2受体的测定在脑血管性痴呆诊治中意义的探讨

目的 :检测脑血管性痴呆患者血清中可溶性白细胞介素 - 2 (s IL- 2 R)水平 ,评价 s IL- 2 R的测定在诊治脑血管性痴呆中的价值。方法 :采用双抗体夹心的 EL ISA法检测脑血管性痴呆患者血清中 s IL- 2 R的水平。结果 :脑血管性痴呆患者血清中的 s IL- 2 R水平较健康对照组显著增高 ,两组比较差异非常显著 (P <0 .0 1)。结论 :检测脑血管性痴呆患者血清中 s IL- 2 R水平 ,有助于了解患者的免疫功能变化及疗效观察     

兔动静脉短路环法诱导组织工程骨支架材料血管生成的初步研究

Objective To compare the effects of 2 vascular carriers, arteriovenous loop and arteri-ovenous bundle, on inducing angiogenesis in coral scaffold of vascularized tissue-engineered bone in animal models.Methods Thirty-six adult male New Zealand rabbits were randomized into 2 even groups.In group A, an arteriovenous loop (AVL) was formed by microsurgical anastomosis at the proximal ends between the femoral poptiteal artery and vein, and placed in the circular side groove of the coral block (6 mm × 8 mm × 10 mm) .In group B, flow-through vessels bundles of both femoral artery and vein were placed in the side grooves of the coral block.All the implants in 2 groups were wrapped by a micro-porous expand-ed-polytetrafluoroethylene (ePTFE) membrane, and fixed subcutaneously by suturing.Evaluation methods included gross morphological observations, histological examinations, India ink perfusion and vascular casting after 2, 4, 6 weeks.The density of blood vessels was analyzed by the statistical software SPSS 10.0.Results All the corals were encased by newly formed fibrovascular tissues in 2 groups.Ink-stained vessels distributed the surfaces and side grooves, and invaded the interspaces of corals.The degree of vascularization increased over the course of experiment.Blood vessel density demonstrated a significant continuous increase between 2 and 6 weeks after implantation in group A.The mean value of blood vessel density in group A (2 weeks 276.60±4.67, 4 weeks 517.20±10.66, 6 weeks 707.00 ±11.87) was significantly higher than in group B (2 weeks 153.60 ±7.16, 4 weeks 269.40±6.80, 6 weeks 279.20±6.53) (P <0.01).Vascular casting showed that in group A, significant blood vessels sprouted from all areas of the loop, espe-cially at the entrance of the arteriovenous pediele where the small tubes were densely interconnected.In group B, however, no blood vessels sprouted from the arteriovenous bundles and only some small vessels grew from the entrance and exit.Conclusions A vascularized coral model can be constructed by inserting an ar-teriovenous loop or an arteriovenous bundle, useful in vascular bone tissue engineering.The former, however, have stronger abilities to induce angiogenesis than the latter.     

采用修正正交分段线性函数求解时滞系统和卷积的方法

应用修正正交分段线性函数的时滞和反向特性，推导出求解时滞系统和卷积的公式。数字仿真表明，它具有计算简单，精度高的优点。