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931.
抗T细胞单克隆抗体对再生障碍性贫血患者TNF和IL—2水平的影响 总被引:2,自引:0,他引:2
为探讨抗T淋巴细胞克隆抗体对再生障碍性贫血患者免疫功能的调节作用,采用放射免疫检测25例AA患者McAb-T治疗前后血清肿瘤坏死因子和白细胞介素-2(IL-2)水平及其中10例周围血单个核细胞体外诱生TNF和IL-2水平的变化。 相似文献
932.
孕妇巨细胞病毒感染对胎儿影响的前瞻性研究 总被引:12,自引:0,他引:12
用酶联免疫吸附试验(ELISA)及聚合酶链反应(PCR)方法对沈阳市450名孕妇进行巨细胞病毒(CMV)筛查,并前瞻性追查到其婴儿100名CMV感染状况。结果孕妇97.11%为既往感染,0.89%为原发感染,11.11%为复发感染,仅2%为易感者。450例中感染组孕妇有畸形儿3例,流产3例,其胎儿感染率与致畸率明显高于对照组。100例母婴检查结果:感染组孕妇所生先天性感染儿比对照组多1.43倍(RR=1.43),感染组有2名低智儿,对照组无。本组早孕原发感染对胎儿危害最大,其宫内传播率为33.3%。感染组孕妇9例感染儿中2例巨细胞包涵体病,7例无症状。为了早期诊断达到优生目的,对孕妇进行CMV筛查是必要的,但筛查过程中发现有活动感染时处理要慎重,最好追查到羊水阳性时考虑终止妊娠。 相似文献
933.
CLINICALSIMULATEDEXPERIMENTONTHESYNDROMISTANDARDSOFPULMONARYSYSTEMDISEASESMulinXuQiouqinWang(HubeiAcademyofTraditionalChinese... 相似文献
934.
Inverted repeats direct repair of adenovirus minichromosome ends 总被引:2,自引:0,他引:2
Adenovirus DNA initiates strand-displacement replication from origins located in identical inverted terminal repetitions (ITRs). Panhandle structures, formed by base pairing between ITRs on the displaced strands, have been proposed as replication intermediates for complementary strand synthesis. We have used a model system, which separates adenovirus replication origin sequences from those involved in panhandle formation, to study the length and sequence integrity of panhandles. By making a series of unidirectional deletion in the panhandle sequence, we show that 31 bp are necessary for panhandle formation. Removal of long stretches of 3'-unpaired nucleotides distal to the panhandle is extremely efficient. Our results argue for the formation of panhandles during adenovirus DNA replication and provide a mechanism for maintaining sequence identity between distantly located inverted repetitions. The size constraint may explain why the adenovirus ITRs are larger than the viral DNA replication origins. 相似文献
935.
Yin Xu Robert W McKenna Jeffrey E Doolittle Christa L Hladik Steven H Kroft 《Applied immunohistochemistry & molecular morphology》2005,13(2):116-123
The clinical and biologic relevance of the t(14;18) and features of germinal center (GC) differentiation in diffuse large B-cell lymphoma (DLBCL) remain controversial. The authors examined the association of t(14;18) with GC-associated markers and clinical features in 44 de novo DLBCLs (22 nodal and 22 primary extranodal). CD10, bcl-2, and bcl-6 were expressed in 50%, 62%, and 54% of cases respectively. There were no significant differences in expression of these markers between nodal and extranodal cases. Coexpression of CD10 and bcl-6 was seen in 12 of 41 cases, and was more frequent in nodal than extranodal DLBCL (9 of 21 vs. 3 of 20; P = 0.05). A CD10+/bcl-6+ phenotype was not significantly associated with bcl-2 expression, stage, complete remission rate, or survival. The t(14;18) was found in 7 of 44 (16%) cases (6 nodal, 1 extranodal; P = 0.09). It was associated with a CD10+/bcl-6+ phenotype (5 of 7 vs. 7 of 27; P = 0.015) and a trend toward more frequent bcl-6 expression (6 of 7 vs. 15 of 34; P = 0.09), but no association with bcl-2 expression, CD10, clinical stage, complete remission, or survival. Among nodal or high-stage (III-IV) DLBCL, cases with the t(14;18) showed a trend toward decreased survival (P = 0.12). 相似文献
936.
Bini TB Gao S Xu X Wang S Ramakrishna S Leong KW 《Journal of biomedical materials research. Part A》2004,68(2):286-295
Tiny tubes with fiber architecture were developed by a novel method of fabrication upon introducing some modification to the microbraiding technique, to function as nerve guide conduit and the feasibility of in vivo nerve regeneration was investigated through several of these conduits. Poly(L-lactide-co-glycolide) (10:90) polymer fibers being biocompatible and biodegradable were used for the fabrication of the conduits. The microbraided nerve guide conduits (MNGCs) were characterized using scanning electron microscopy to study the surface morphology and fiber arrangement. Degradation tests were performed and the micrographs of the conduit showed that the degradation of the conduit is by fiber breakage indicating bulk hydrolysis of the polymer. Biological performances of the conduits were examined in the rat sciatic nerve model with a 12-mm gap. After implantation of the MNGC to the right sciatic nerve of the rat, there was no inflammatory response. One week after implantation, a thin tissue capsule was formed on the outer surface of the conduit, indicating good biological response of the conduit. Fibrin matrix cable formation was seen inside the MNGC after 1 week implantation. One month after implantation, 9 of 10 rats showed successful nerve regeneration. None of the implanted tubes showed tube breakage. The MNGCs were flexible, permeable, and showed no swelling apart from its other advantages. Thus, these new poly(L-lactide-co-glycolide) microbraided conduits can be effective aids for nerve regeneration and repair and may lead to clinical applications. 相似文献
937.
Cerebral ischemia causes an excess release of glutamate, which can injure neurons. The striatum is one of the important regions vulnerable to hypoxia and ischemia. Using push–pull perfusion technique, we investigated the regulatory role of γ-aminobutyric acid (GABA) and its receptors in modifying the amount of glutamate in rat striatum with ischemia. Perfusion with exogenous GABA (1 mM) inhibited cerebral ischemia-induced glutamate release by as much as 47%. We further characterized relative roles of subtype receptors of GABA on glutamate release by using pharmacological tools. While baclofen (500 μM), a GABAB receptor agonist, suppressed ischemia-induced glutamate release by 52%, GABAB receptor antagonist saclofen (500 μM) failed to produce a significant increase of glutamate release. The GABAA receptor agonist muscimol (500 μM) also reduced by 38% the release of glutamate induced by cerebral ischemia but the GABAA receptor antagonist bicuculline (500 μM) had very little effect. The present study demonstrates that the excessive release of glutamate or the overly activated glutamate receptor, triggered by cerebral ischemia, can be down-regulated by exogenous GABA or by increased activity of GABA receptors, especially the presynaptic GABAB receptors, which might be one of the important mechanisms to protect against striatum neuronal damage from over stimulation by excessive glutamate during ischemia. 相似文献
938.
939.
Todd S Xu J Millar BC Moore JE Crowe M Raoult D Harrison T Hill C Douglas J 《British journal of biomedical science》2004,61(4):190-193
Members of the genus Bartonella are increasingly recognised as a cause of culture-negative endocarditis, particularly in those patients with underlying risk factors (e.g., homelessness and alcoholism (B. quintana) or valvulopathy and cat ownership (B. henselae). The aortic and mitral-valves are most commonly involved. Here, a case is reported of culture-negative right-sided endocarditis, without any of the above risk factors, due to Bartonella sp. in a 69-year-old man who presented with acute renal failure. The diagnosis was made using a broad-range 16S rRNA polymerase chain reaction (PCR) technique and direct automated sequencing on a peripheral blood sample, which was subsequently confirmed serologically. A review of the literature on Bartonella endocarditis is also presented. Molecular laboratory methods using peripheral blood or blood cultures may be very useful in the diagnosis of causal agents in culture-negative endocarditis and add further support to the recently inclusion of molecular (PCR) diagnosis, as a major Duke's criterion, for the diagnosis of infective endocarditis. 相似文献
940.
Inborn errors of metabolism discovered in Asian department of pediatrics and mental retardation research center 总被引:6,自引:0,他引:6
Zhang C Xu K Dave UP Wang Y Matsumoto I 《Journal of chromatography. B, Biomedical sciences and applications》2000,746(1):41-49
To heighten the effectiveness of chemical diagnosis for inborn errors of metabolism (IEM) using urease pretreatment and GC-MS analysis, a sample collection and transportation method was contrived. The resulting "filter paper set" allows simple urine collection and transportation, and enables anyone from anywhere to receive the GC-MS analysis without the limitations of place or time. Using filter paper sets, high-risk screening of undiagnosed children or mentally retarded children with unknown cause was conducted in cooperation with hospitals and universities in several Asian countries. During 8 months 203 patients from China and India were analyzed and 20 cases of IEM were chemically diagnosed. These diagnoses greatly contributed to the treatment of children with intractable diseases who lived in Asian countries where analytical techniques and facilities for IEM were not sufficient. 相似文献