海南黎族跳娘舞的健身价值

目的:评价跳娘舞者运动负荷程度,分析其健身价值。方法:①2003-03/07对海南省五指山市、陵水县、保亭县3个黎族市县的乡镇跳娘舞者120人,其中男80人,女40人,年龄20～40岁。均身体健康,且对检测项目知情同意。②跳娘舞共有8个表演程序基本动作:跳步,跳踢步,三步摆胯,二步摆胯,甩手拭汗,摊掌跳摆,甩手祈福,甩手闪臂,拍手跳。③以主观心理感觉等级表等级作为运动时心理负荷的标志,该表按自我感觉分为6～20级,级数越高,则表明被试感觉越累,运动强度越大。运动处方的适宜强度应为11～14级(心率110～140次/min)。④用芬兰产的Polar遥测心率仪对跳娘舞者心率进行测定。结果:跳娘舞者97人(81%)心率为110～140次/min,是适宜的运动负荷程度。结论:大多数跳娘舞者运动负荷程度适宜,是健身效果较好的运动。     

《数字化卒中单元管理系统》数据库网络版的研究与开发

目的:充分利用现有的计算机网络资源,研究和开发卒中单元计算机管理软件。方法:客户端利用MicrosoftVisualBasic6.0作为计算机语言编写系统前台软件,即《数字化卒中单元管理系统》,给用户提供了人机对话界面。服务器端采用甲骨文公司ORACLE数据库作为系统数据库。通过《数字化卒中单元管理系统》调用后台服务器ORACLE数据库中的数据。结果:成功编写完成了《数字化卒中单元管理系统》软件,是一套网络化、高效、方便快捷、统一有序的卒中单元计算机管理软件。结论:《数字化卒中单元管理系统》为卒中单元的临床和研究积累数据,为组织化卒中医疗提供网络化管理,是建立和运作卒中单元的数字化临床路径,简化和方便了卒中单元的推广和运作。     

人食管上皮酸反流损伤的早期超微结构改变

学改变.本研究的目的是确定此种改变是否也是人食管酸反流损伤的一种早期表现.方法用透射电镜检查10例正常对照组,12例胃食管反流病(GERD)患者(经24 h pH监测和压力测定诊断为GERD)的内镜活检标本(标本均取自食管下端5 cm内、内镜下无异常的粘膜),用图象分析仪测量每例标本透射电镜照片的细胞间隙.结果胃食管反流病患者组的细胞间隙(1.27±0.77)μm大于正常对照组细胞间隙(0.49±0.08)μm,P＜0.01,前者有9例有细胞间隙≥2.5 μm,而正常对照组无一例有细胞间隙≥2.5μm.结论细胞间隙增大是人食管酸反流损伤的一种早期表现.     

蟒蛇胆化学成分研究

从蟒蛇(Python molurus bivittatus Schlegel)胆中,用柱色谱方法提取分离了化合物Ⅰ和Ⅱ。其中Ⅰ为牛磺去氧胆酸钠。化合物Ⅱ,经元素分析及光谱(IR,¹HNMR,¹³CNMR,¹³C-¹H COSY和MS)分析和化学反应,证明是一个新的化台物,命名为牛磺蟒胆酸钠。     

以牛血清白蛋白为中间载体的血卟啉衍生物与抗胃癌单克隆抗体交联物的抗肿瘤作用

以牛血清白蛋白为中间载体,将血卟啉衍生物(HPD)与抗胃癌单克隆抗体3H11交联。交联物3H11-BSA-HPD的克分子比为1:1:200。本文对3H11-BAS-HPD的体内外抗肿瘤作用进行了研究,并与直接交联物3H11-HPD进行比较。3H11-BSA-HPD和3H11-HPD对胃癌靶细胞BGC-823的细胞毒效应相似,并均明显比游离HPD强。在接种靶细胞(2×10~5细胞/只)的裸鼠中,对照组和HPD组均于接种后13天内形成瘤块,而间接和直接交联物处理组在34天实验期内仅有1/6动物形成肿瘤。结果表明3H11-BSA-HPD和3H11-HPD在HPD相等剂量下,具有相似的导向杀伤肿瘤细胞的作用。     

浙贝素的结构测定

从浙江产贝母(Fritillaria thunbergii Miq)地上部分分离出一个新的3,7-二氧杂环[3,3,O)辛酮-6类化合物,经光谱(¹HNMR,¹³CNMR,HRMS,EI-MS)及X-单晶衍射数据的分析,确定其结构为2-(3′,5′-dimethoxy-4′-hydroxyphenyl)-3,7-dioxabicyclo[3,3,O]octan-6-one,定名为浙贝素(zhebeiresinol)。     

羧乙基锗倍半氧化物对正常和异丙基肾上腺素损伤培养乳鼠心肌细胞的影响

羧乙基锗倍半氧化物(Ge—132)能促进培养乳鼠心肌细胞DNA和RNA的合成,提高超氧化物歧化酶(SOD)的活性,维持细胞膜结构的稳定。异丙基肾上腺素可使心肌细胞乳酸脱氢酶(LDH)释放增加,细胞搏动停止,超微结构显示肌膜和线粒体严重受损。Ge—132可显著减少异两基肾上腺素引起心肌细胞释放LDH,维持细胞的搏动功能和超微结构的完整。     

大丁草中抗菌活性成分的研究:人肠道微生物对大丁甙及其类似物的代谢产物

本文论述了人肠道微生物对大丁草中三种香豆精甙类化合物的代谢产物研究。鉴定了其中两个主要代谢物的结构和代谢物随时间变化的情况。发现了一种香豆精的新型代谢方式。从而初步阐明这类化合物在体内发挥抑菌作用的原理。为今后更好地应用该植物及主要活性成分提供了理论依据。     

Serum form of the erythropoietin receptor identified by a sequence- specific peptide antibody [see comments]

The present investigation was undertaken to search for soluble forms of the erythropoietin receptor in human serum using polyclonal antibody against an amino terminal peptide sequence in the extracellular domain. This sequence was located adjacent to the amino terminus at residues 25- 38. When this antibody was used for Western blots of solubilized membranes from nucleated bone marrow cells, a protein consistent with native erythropoietin receptor was seen. Purified soluble ectodomain of the erythropoietin receptor displayed appropriate reactivity with this antibody. When sera from normal subjects and patients with a range of hematologic disorders were examined by Western blotting, a protein with a molecular mass of 34 Kd was detected in sera from patients with enhanced erythropoiesis including sickle cell anemia, thalassemia, and megaloblastic anemia. This protein was rarely detected in normal serum but appeared when normal subjects were treated with recombinant erythropoietin and disappeared after full treatment of patients with megaloblastic anemia due to vitamin B12 deficiency. The protein was not detected after myeloablation for bone marrow transplantation but appeared with marrow engraftment. Reactivity of this protein with the peptide antibody was competitively inhibited by the amino terminal peptide sequence. An additional 48 Kd protein was detected that showed minimal variation in intensity with differing degrees of erythropoietic activity. Detection of this protein could not be inhibited by the addition of synthetic peptide. Our findings indicate the presence of a soluble form of the erythropoietin receptor related to the extracellular domain that is highly correlated with enhanced erythropoiesis.     

Biochemical analysis of specific histamine HI and H2 receptors on lymphocytes

It is increasingly clear that histamine mediates a variety of lymphocyte functions. Further understanding of these mechanisms requires a method for the analysis of histamine membrane receptors on the lymphocyte surface. We report now a biochemical technique for the identification and quantitation of specific histamine H1 and H2 receptors of lymphocytes. The method can be performed on small numbers of formaldehyde-fixed cells. The data this assay yields, together with that resulting from the flow cytometric analysis of histamine receptor distribution (a technique we have previously described), will be a powerful tool in the study of histamine mediation of lymphocyte function.