含达雷妥尤单抗联合方案挽救治疗早期前体T淋巴母细胞白血病一例

患者,男,33岁,因"淋巴结肿大1个月"于2019年10月就诊于我院。血常规示:WBC 1.25×109/L,HGB 76 g/L,PLT 44×109/L;骨髓涂片:原始幼稚淋巴细胞93.5%;骨髓免疫分型:异常细胞占非红细胞的94.1%,主要表达cCD3、CD5、CD7、CD34、CD38,部分表达CD13、CD33,不表达CD2、mCD3、CD4、CD8、CD79a、cMPO,免疫表型符合异常早期前体T淋巴母细胞白血病(ETP-ALL);染色体核型:46,XY,-7,+der(1;19)(q10;q10)[2]/45,X,-7,+der(1;19)(q10;q10),-Y[2]/46,XY[10];骨髓RNA测序:未见融合基因,FLT3-ITD突变阳性(等位基因比1.5);腰椎穿刺结果正常。综合上述结果,确诊为ETP-ALL。先后接受1个疗程VDCD(长春地辛+柔红霉素+环磷酰胺+地塞米松)方案和1个疗程CAG(阿柔比星+阿糖胞苷+粒细胞集落刺激因子)方案诱导化疗,均未达到完全缓解。     

Proteomic Analysis of Human Milk Reveals Nutritional and Immune Benefits in the Colostrum from Mothers with COVID-19

Despite the well-known benefits of breastfeeding and the World Health Organization’s breastfeeding recommendations for COVID-19 infected mothers, whether these mothers should be encouraged to breastfeed is under debate due to concern about the risk of virus transmission and lack of evidence of breastmilk’s protective effects against the virus. Here, we provide a molecular basis for the breastfeeding recommendation through mass spectrometry (MS)-based proteomics and glycosylation analysis of immune-related proteins in both colostrum and mature breastmilk collected from COVID-19 patients and healthy donors. The total protein amounts in the COVID-19 colostrum group were significantly higher than in the control group. While casein proteins in COVID-19 colostrum exhibited significantly lower abundances, immune-related proteins, especially whey proteins with antiviral properties against SARS-CoV-2, were upregulated. These proteins were detected with unique site-specific glycan structures and improved glycosylation diversity that are beneficial for recognizing epitopes and blocking viral entry. Such adaptive differences in milk from COVID-19 mothers tended to fade in mature milk from the same mothers one month postpartum. These results suggest that feeding infants colostrum from COVID-19 mothers confers both nutritional and immune benefits, and provide molecular-level insights that aid breastmilk feeding decisions in cases of active infection.     

普通高校学报目标定位及发展思路探讨

针对我国高校学报发展面临的一些问题,从办刊目标定位和发展思路两个方面进行了初步的分析和探讨。高校学报必须明确办刊的目标定位,从树立创新意识和加强质量控制两个方面实现学报的优势发展。     

Microstructure of Dolostones of Different Geological Ages and Dedolomitization Reaction

Dolostone is widely distributed and commonly used as concrete aggregates. A large number of studies have shown that there are significant differences in the expansibility of different dolostones, and the key factors determining the expansibility of alkali carbonate rocks have not been clarified. In this paper, rocks were selected from five different geological ages: Jixianian, Cambrian, Ordovician, Devonian, and Triassic ages. The ordering degree and the content of MgCO₃ of dolomites in rocks of different geological ages were determined by X-ray diffraction (XRD). The degree of dedolomitization reaction in rocks cured in 80 °C, 1 mol/L NaOH solution was determined by quantitative X-ray diffraction (QXRD). The morphology of dolomites in rocks was determined by a polarizing microscope. The products of the dedolomitization reaction were determined by field emission electron microscopy (FESEM-EDS). According to the test results, the following conclusions are drawn. There is a good positive correlation between ordering degree and the molar fraction of MgCO₃ of dolomites. When the MgCO₃ mole fraction of dolomites varies from 47.17% to 49.60%, the higher the MgCO₃ mole fraction, the greater the ordering degree of dolomite. By analyzing the degree of the dedolomitization reaction of different dolostone powders cured at 80 °C in 1 mol/L NaOH solution, it is found that the older the geological age of dolostone, the slower the dedolomitization reaction rate and the lower the degree of dedolomitization reaction. The lower the ordering degree of dolomite crystal in the same geological age, the faster the rate of dedolomitization reaction and the higher the degree of dedolomitization reaction.     

槲皮素对肺腺癌细胞系A549细胞中凋亡相关因子Bcl-2表达的影响

目的探讨槲皮素对肺腺癌细胞系A549细胞中凋亡相关因子Bcl-2表达的影响。方法采用RT-PCR与Western Blot技术检测槲皮素在不同时间、不同浓度作用A549细胞后的Bcl-2 mRNA和蛋白表达的变化。结果槲皮素作用A549细胞后,A549细胞的Bcl-2 mRNA和蛋白表达水平逐渐降低,并且呈现出一定的时间和浓度依赖性。结论槲皮素诱导A549细胞调亡可能通过下调凋亡抑制基因Bcl-2的表达而起作用。     

肠道益生菌补充对酒精高糖高脂致肝损伤大鼠肝功能和肠道菌群的影响

目的 探究肠道益生菌补充对酒精、高糖、高脂致肝损伤大鼠肝功能和肠道菌群的影响.方法 高糖高脂饮食联合白酒灌胃处理制备酒精、高糖、高脂致肝损伤大鼠模型,并分为空白对照组、模型组、低剂量肠道益生菌组(20×108 CFU/kg)和高剂量肠道益生菌组(40×108 CFU/kg).灌服白酒7周后,算大鼠脏体比、血清乙醇体积分...     

年龄相关性黄斑变性的诊断和影像学检查新进展

年龄相关性黄斑变性(age-related macular degene-ration,AMD)是一种多见于中老年人群(≥50岁)的致盲眼病,常累及视网膜黄斑区,引起中心视力进行性下降.该病早期的临床征象包括玻璃膜疣和视网膜色素上皮(retinal pigment epithelium,RPE)异常,晚期可以出现新生血...     

Association of Dietary Inflammatory Potential with Blood Inflammation: The Prospective Markers on Mild Cognitive Impairment

Inflammation is known as an important mechanism of cognitive dysfunction. Systemic immune inflammation index (SII) and system inflammation response index (SIRI) are two blood inflammatory markers, which are related to many chronic diseases including cognitive impairment. It is recognized that dietary inflammatory index (DII), which is used to estimate the overall inflammatory potential of diet, may be related to mild cognitive impairment (MCI) as well. This study aimed to explore the relationship between SII, SIRI and DII, as well as the role of these inflammatory indexes on MCI in elderly people. A total of 1050 participants from Beijing were included. Neuropsychological tests were used for cognitive evaluation. Energy-adjusted DII scores were calculated based on semi-quantitative food frequency questionnaire. Blood samples were tested for calculating SII and SIRI. Log-binomial regression models were used to estimate the correlation of indexes. After adjusting demographic characteristics, SII and SIRI in MCI individuals were higher than controls (p ≤ 0.001). DII, SII and SIRI had positive relationship with MoCA scores (p < 0.005). DII also correlated with SIRI in MCI (β = 0.11, p = 0.031). Higher DII and SIRI could definitely increase the risk of MCI, as well as DII and SII (p < 0.005). In conclusion, DII was positively correlated with blood inflammation. The elderly with higher level of DII and SIRI, or DII and SII could be considered as people with higher risk of developing MCI.     

槲皮素对LPS刺激肺上皮细胞核转录因子NF-κB表达的影响

目的研究槲皮素对肺上皮细胞株(A549细胞)不同时段表达核转录因子(NF-κB)的影响。方法用LPS建立体外A549细胞损伤模型,用免疫印迹法(Western Blot)观察不同时间A549细胞核转录因子NF-κB的表达。结果A549细胞在LPS刺激下,NF-κB表达水平逐渐升高,呈现出一定的时间依赖性,而槲皮素能显著抑制其表达。结论NF-κB是炎症反应中重要的核转录蛋白,参与一系列炎症介质的表达。因此,槲皮素抑制LPS诱导A549细胞产生NF-κB,提示槲皮素可能通过对炎症因子负性调控而发挥其抗炎作用。     

PTZ点燃癫痫大鼠海马BMP4的表达研究

目的观察骨形成蛋白4（BMP4）基因在戊四氯（PTZ）点燃癫痫大鼠海马中的表达,并探讨其与癫痫发病机制的关系。方法将40只成年雄性SD大鼠随机分为对照组和实验组。实验组采用PTZ点燃癫痫大鼠,按点燃进程,又随机分为Ⅰ级组、Ⅲ级组、Ⅳ级组和Ⅴ级组。用地高辛标记特异性寡核苷酸探针原位杂交组织化学技术和图像分析技术,观察海马BMP4表达的变化。结果发现应用PTZ点燃后,Ⅱ级以上大鼠海马区BMP4的表达增加。Ⅲ和Ⅳ级大鼠海马CA3及DGBMP4的表达明显高于对照组（P〈0．01）;Ⅴ级大鼠在发作后海马CA3及DGBMP4的表达呈逐渐下降。结论BMP4可能参与了癫痫的发生、发展过程。