中性粒细胞粘附分子CD11b表达对新生儿败血症早期诊断价值的研究

目的　观察新生儿败血症中性粒细胞粘附分子CD11b表达的规律 ,并评价其在新生儿败血症早期诊断中的价值。方法　将 5 1例临床疑似败血症的新生儿根据其临床表现及WBC、PLT、血浆CRP和未成熟中性粒细胞数与中性粒细胞总数比值 (I/T)四项指标 ,分为败血症和可疑败血症两组。采用全血流式细胞术检测患儿和 15例正常对照组新生儿中性粒细胞CD11b的平均荧光强度 (MFI)。结果　败血症组 2 3例 ,可疑败血症组 2 8例。两组中性粒细胞CD11b分别为 (32 0± 189)、(4 5 6± 2 13)MFI,均显著低于正常对照组的 (10 90± 338)MFI(t分别为 - 9 0 1、- 7 5 6 ,P均 <0 0 0 1) ,败血症组又低于可疑败血症组 ,差异有显著性 (t=- 2 39,P <0 0 5 )。高CRP组患儿CD11b为 (2 11± 16 4 )MFI,低于低CRP组的 (5 0 5± 2 6 5 )MFI,差异有显著性 (t=2 6 4 ,P <0 0 5 )。中性粒细胞CD11b≤ 6 0 0MFI对疑似败血症新生儿诊断的敏感性、特异性、阳性和阴性预测值分别为 86 3%、10 0 %、10 0 %、6 8 2 % ,CD11b检测阳性率为 86 3% ,高于血培养的阳性率 (17 6 % ) ,差异有显著性 (χ2 m=31 2 ,P <0 0 5 )。结论　新生儿败血症中性粒细胞粘附分子CD11b表达下调 ,其下调与感染严重程度有一定关系。中性粒细胞CD11b的动态检测对早期     

Genetic alterations in primary cutaneous CD30+ anaplastic large cell lymphoma

Primary cutaneous CD30+ anaplastic large cell lymphoma (C-ALCL) represents a distinct clinical subtype of CD30+ anaplastic large cell lymphomas. The etiology and underlying molecular pathogenesis of C-ALCL remain unclear. This study aimed to investigate genetic changes in C-ALCL. Comparative genomic hybridization (CGH) analysis of 23 DNA samples from 15 C-ALCL cases identified chromosome imbalances (CI) in 10 samples from eight cases (43%). The mean number of CI per sample was 2.09 +/- 3.86, with gains (2.00 +/- 3.85) more common than losses (0.09 +/- 0.29). The most frequent CI were gains of 1/1p and 5 (50%) and 6, 7, 8/8p, and 19 (38%). Microarray-based CGH analysis of six DNA samples from five cases with CI revealed genomic imbalances (GI) in all of the cases studied. This included oncogene copy number gains of FGFR1 (8p11) in three cases, and NRAS (1p13.2), MYCN (2p24.1), RAF1 (3p25), CTSB (8p22), FES (15q26.1), and CBFA2 (21q22.3) in two cases. Real-time PCR analysis of nine DNA samples from eight cases with CI and GI detected amplifications of CTSB and RAF1 in seven cases (88%), REL (2p13p12) and JUNB (19p13.2) in six cases (75%), and MYCN and YES1 (18p11.3) in four cases (50%). Immunohistochemical staining of paraffin sections from six cases demonstrated expression of JUNB protein in five cases and BCL2 in three cases. These results reveal a consistent pattern of genetic alterations in C-ALCL and provide the molecular basis for further investigation of this disease.     

Microcapsules with improved mechanical stability for hepatocyte culture

Packed-bed or fluidized-bed bioreactor filled with microencapsulated hepatocytes has been proposed as one of the promising designs for bioartificial liver assist device (BLAD) because of potential advantages of high mass transport rate and optimal microenvironment for hepatocyte culture. Recently, we have developed a microcapsule system for the encapsulation of hepatocytes. The microcapsules consist of an inner core of modified collagen and an outer shell of terpolymer of methyl methacrylate, methacrylate and hydroxyethyl methacrylate. Cells encapsulated in these microcapsules exhibit enhanced cellular functions. Improving the mechanical stability of the microcapsules to withstand the shear stress induced by high perfusion rate would be crucial to the success of BLAD applications. In this study, we investigated the effects of terpolymer molecular weight (M(w)) on the mechanical property of these microcapsules and the differentiated functions of encapsulated hepatocytes. Six terpolymers with different M(w) were synthesized using radical polymerization in solution by adjusting the reaction temperature and the initiator concentration. All the terpolymers formed microcapsules with the methylated collagen. While the terpolymer M(w) had little effect on the capsule membrane thickness and permeability of serum albumin, the mechanical property of the microcapsules was significantly improved by the higher M(w) of the terpolymer. Differentiated functions of the hepatocytes cultured in the microcapsules, including urea synthesis, albumin synthesis and cytochrome P450 metabolic activity, were not significantly affected by the terpolymer M(w).     

产后抑郁症患者血浆孤啡肽及5-羟色胺水平变化的意义

目的　探讨产后抑郁症患者血浆中孤啡肽及 5 羟色胺水平变化与产后抑郁症发病的关系。方法　采用放射免疫法测定 2 5例正常产妇 (对照组 )及 2 1例产后抑郁症患者 (抑郁症组 )血浆中孤啡肽及 5 羟色胺水平。结果　 (1)抑郁症组血浆孤啡肽水平为 (2 8 5± 5 8)ng/L ,对照组血浆孤啡肽水平为 (10 4± 3 7)ng/L。抑郁症组血浆孤啡肽水平显著高于正常组 ,两组比较 ,差异有极显著性 (P <0 0 1)。 (2 )抑郁症组血浆 5 羟色胺水平为 (1 0± 0 3) μmol/L ,对照组为 (1 4± 0 4 ) μmol/L。抑郁症组血浆 5 羟色胺水平低于对照组 ,两组比较 ,差异有显著性 (P <0 0 5 )。 (3)抑郁症组血浆孤啡肽水平与 5 羟色胺水平呈显著负相关 (r=- 0 5 71,P <0 0 5 )。结论　产后抑郁症患者血浆孤啡肽水平变化与产后抑郁症的发生密切相关。     

Clinical findings among young women with genital human papillomavirus infection

OBJECTIVE: The purpose of this study was to identify clinical signs and symptoms associated with detection of human papillomavirus (HPV) DNA in the female genital tract. STUDY DESIGN: A total of 516 university students (18 to 24 years old) enrolled in a cohort study that included the collection of genital specimens for HPV DNA testing every 4 months for up to 4 years. Reported symptoms and objective clinical findings of women with and without HPV DNA were compared by multivariate analysis. RESULTS: Acute and persisting HPV infections were not associated with discharge, itching, burning, soreness, or fissures. Clinical evidence of genital warts was statistically associated only with HPV types 6 and 11. Detection of any HPV DNA was associated with bacterial vaginosis (BV). Furthermore, a time lag analysis suggests that HPV infection usually precedes detection of BV. CONCLUSION: Most women who acquire genital HPV infection are asymptomatic; some, however, are at increased risk for BV.     

Cloning and characterization of a novel splice variant of the brain-specific protein densin-180

Densin-180 is a brain-specific synaptic protein of the o-sialoglycoprotein family. It functions in specific adhesion between presynaptic and postsynaptic membranes at glutamatergic synapses. We have cloned a novel splice variant of densin-180 from a fetal brain cDNA library and termed it LRRC7. It is located on 1p31 and consists of 8 exons. It encodes a putative protein of 216 amino acids which contains a nuclear localization signal and four leucine-rich repeats (LRRs). LRRs are 20-29 residue sequence motifs present in a number of proteins with diverse functions. Expression analysis of LRRC7 shows it is expressed ubiquitously while its splice variant densin-180 is brain-specific.     

Identification and expression analysis of a novel splice variant of human Sprouty1 gene

Sprouty (SPRY) was first identified in a genetic screen in Drosophila to be an antagonist of fibroblast growth factor (FGF) and epidermal growth factor (EGF) signaling, seemingly by inhibiting the Ras/MAP kinase pathway. During large scale DNA sequencing of the human fetal brain cDNA library, we cloned a novel splice variant of human Sprouty1 gene, and termed it human Sprouty1b (SPRY1b). It has the same deduced protein as Sprouty1a, which has been reported. And like other members of Sprouty family, SPRY1b deduced protein also has a C-terminal cysteine-rich region. According to the search against human genome database, SPRY1b was mapped to 4q25-28. Expression analysis of SPRY1a and SPRY1b shows that they are hardly expressed in adult human, but have different expression patterns in fetus, which confirmed that SPRY1 is an important gene during fetal development.     

Utilization of moiré interferometry to study the strain distribution within multi-layer thermoplastic elastomers

The use of soft elastomeric cushion form bearings as an alternative material to ultra-high molecular weight polyethylene (UHMWPE) has been proposed in the literature as providing enhanced lubrication and lower friction. However, the abrupt change in stiffness between the bearing's soft contact layer and its rigid support substrate results in high shear stresses and leads to the debonding of the soft layer from the substrate. The use of functionally modulus-graded material has been proposed as a solution to this problem. This paper investigates the use of moiré interferometry to study the strain distribution within and across the interfaces of multi-layer elastomeric samples, which were fabricated as models for functionally modulus-graded materials. While this technique has been widely used to study the strain distribution in rigid materials and composites, this paper represents the first report of its application to low-modulus polymers at temperatures where they exhibit significant viscoelastic behavior. The results presented clearly demonstrate that the moiré interferometry technique can be successfully applied in the field of low-modulus elastomeric materials. The analysis of the moiré patterns suggests that the soft elastomeric material under the contact point was subjected to a compressive epsilonx, and was pushed sideways. The analysis also showed that the maximum shear strain occurred where the deformation was constrained, which could possibly lead to a local fatigue failure in the sample.