高效液相色谱法固相酶检测系统测试生物样本中胆碱与乙酰胆碱的研究

以大孔径玻璃微粒为载体,将生物酶化学键合于玻璃体,制备成Φ5×50mm短柱串联于色谱分离柱后,配合电化学检测器,构建了高效液相色谱固相酶检测平台。采用了AtlantisC18反相色谱柱(一种适用于强极性化合物分析的反相色谱柱)成功分离了胆碱与乙酰胆碱,并特异、灵敏地获取检测信号。研究目标组份在AtlantisC18柱的保留特性,比较了流动相不同组成对酶促反应的影响,优化了色谱条件。色谱条件:AtlantisC18柱,电化检测,流动相:Na2HPO450mmol/L,检测限(S/N=2)在1.1￣3.4pmol以下,绝对回收率均>95.50%,样品日内相对标准差<1.49%。方法精密、准确。     

三种材料修复牙齿楔状缺损的临床疗效观察

目的:选择较理想的修复牙齿楔状缺损的材料和方法。方法:门诊选择141例,453颗患牙随机分为3组,分别用GC Fuji II型玻璃离子、Dyract AP复合体和GC Fuji II型玻璃离子水门汀加Charisma光固化复合树脂夹层技术修复深型楔状缺损,于修复后1、2年随访,行临床疗效观察。结果:甲组(GC Fuji II型玻璃离子)1年成功率86.67%,2年成功率75.05%;乙组(Dyract复合体)1年成功率91.61%,2年成功率87.30%;丙组(夹层技术)1年成功率93.57%,2年成功率89.43%。丙组成功率高于乙组,差异无显著性,丙组和乙组成功率均高于甲组,差异有显著性。结论:丙组(夹层技术)、乙组(Dyract AP复合体)用于楔状缺损修复的临床疗效较单纯用GC Fuji II型玻璃离子修复成功率高(P<0.05),是较理想的楔状缺损修复材料或方法。     

The Protective Effect of Rosuvastatin on Ischemic-Brain Injury and Its Mechanism

The statins, which lower plasma cholesterol levels, are 3-hydroxy-3-methylglutaryl coenzyme A (HMGCoA) reductase inhibitors. To date, stains have developed to third generation, which include five commonly used stains: lovastatin, pravastatin, simvastatin, fluvastatin and atorvastatin. Recently, a new statin, named rosuvas-tatin, was used in the third stage of clinical trial. Rosu-vastatin, in contrast to most other statins, has the more powerful capability to lower plasma cholesterol levels,…     

建立中医概念的三维立体模式的可行性及意义

分析了当前中西医结合的误区,提出中医概念三维立体模式网络。按照客观到主观提炼的原则将中医概念分为若干层次,最高一层为中医纲领,底层为中医、西医基本概念,各个概念之间通过层次模式网络发生关联,最终形成的立体三维网将成为开放的、可以吸收新概念的中医概念模式网。     

In vitro and in vivo antifungal activities of the eight steroid saponins from Tribulus terrestris L. with potent activity against fluconazole-resistant fungal pathogens

Antifungal activity of natural products is being studied widely. Saponins are known to be antifungal and antibacterial. We have isolated eight steroid saponins from Tribulus terrestris L. , namely TTS-8, TTS-9, TTS-10, TTS-11, TTS-12, TTS-13, TTS-14 and TTS-15. TTS-12 and TTS-15 were identified as tigogenin-3-O-β-D-xylopyranosyl（1→ 2）-[-β-D-xylopyranosyl（ 1 → 3 ） 3-β- D-glucopyranosyl （ 1 → 4 ）- 1- α-L-rhamnopyranosyl （ 1 → 2 ） 3-β-D-galactopyranoside and tigogenin-3-O-β-D-glucopyranpyranosyl（1→2）-[-β-D-xylopyranosyl（1→ 3）3-β-D-glucopyranosyl（1→4）-β-D-galactopyranoside, respectively. The in vitro antifungal activities of the eight saponins against six fluconazole-resistant yeasts, Candida albicans, Candida glabrata, Candida para psilosis , Candida tropicalis , Candida krusei , and Cryptococcus neo f ormans were studied using microbroth dilution assay. The results showed that TTS-12 and TTS-15 were very effective against several pathogenic candidal species and C. neoformans in vitro. It is noteworthy that TTS-12 and TTS-15 were very active against fluconazole-resistant C. albicans （MIC80 = 4.4, 9.4 mg/ml）, C. neoformans （MIC80 =10.7, 18.7 mg/ml） and inherently resistant C. krusei （MIC80 =8.8, 18.4 mg/ml）. So in vivo activity of TTS-12 in a vaginal infection model with fluconazole-resistant C. albicans was studied in particular. Our studies revealed TTS-12 also showed in vivo activities against fluconazole-resistant yeasts. In conclusion, steroid saponins TTS-12 and TTS-15 from Tribulus terrestris L. have significant in vitro antifungal activity against fluconazole-resistant fungi, especially TTS-12 also showed in vivo activity against fluconazole-resistant C. albicans.     

128株嗜麦芽窄食单胞菌在院内感染中的耐药性分析

目的研究唔麦芽窄食单胞菌的感染危险因素及其耐药特征，提高临床对嗜麦芽窄食单胞菌防治水平。方法对128例嗜麦芽窄食单胞菌感染者的临床资料进行统计分析，并用K-B法测其耐药情况。结果嗜麦芽窄食单胞菌占非发酵革兰阴性杆菌23．0％，居铜绿假单胞菌之后，列第二位；药敏试验表明：该菌广泛耐药，敏感率〉50％者仅有复方新诺明、替卡西林／克拉维酸与头孢哌酮／舒巴坦；在感染患者中，有90．3％患有基础病，97．0％先期使用过广谱抗生素，75．7％曾接受侵入性检查和治疗。结论嗜麦芽窄食单胞菌已成为医院内感染重要致病菌之一，多发生在免疫功能低下的老年、慢性疾病、接受激素及长期抗菌药物治疗的患者，施行各种侵入性检查和治疗的患者也是感染的高危人群，嗜麦芽窄食单胞对多种抗生素耐药，一旦感染，治疗颇为困难。     

半夏药材中蛋白质IEF电泳图谱的研究

目的通过对半夏及其近缘植物的蛋白质电泳图谱比较,研究半夏药材的电泳鉴别方法。方法应用等电聚焦(isoe lectrofocus ing,IEF)技术,对不同产地半夏及其近缘植物块茎进行比较。结果半夏具有稳定的IEF电泳图谱,显示具有8条特征蛋白质带,并测定了其等电点(P I)值,半夏与其几种近缘植物的IEF谱有明显的区别。结论半夏IEF电泳图谱可作为半夏药材的鉴别依据,半夏特征蛋白质的P I值为半夏的鉴定和蛋白质分离纯化提供了参数。