老年垂体腺瘤的经蝶显微手术治疗

目的：对22例老年人垂体腺瘤的经蝶显微手术治疗,探讨老年性垂体腺瘤的临床特点及治疗原则,方法：本组老年人垂体腺瘤均经血清激素检查,CT或MRI扫描确诊,采用经蝶显微手术治疗,术后治疗特别注意水和电解质的平衡,结果：无分泌功能腺瘤17例,肿瘤全切12例,次全切7例,大部分切除3例,无手术死亡,无并发症死亡。在平均3．1年随访中,20例恢复良好,2例症状复发,结论：老年性垂体腺瘤绝大部分为无功能性,经蝶入路显微手术切除是一种安全,有效的方法,但要注意手术适应症的选择和术后体液,电解质平衡。     

单糖特征分析在腹膜透析相关性腹膜炎病原菌诊断中的价值

目的 单糖是病原菌的重要组成成分,不同类型的病原菌具有不同的单糖特征。本研究拟通过高效液相色谱法测定腹膜透析液中单糖的水平,探讨单糖检测对腹膜炎及引起腹膜炎的病原菌的诊断价值。方法 收集2020年10月01日至2021年10月31日到青岛大学附属医院西海岸肾病科住院的腹膜透析患者的腹膜透析液样本90例,其中包括无腹膜炎样本52个,腹膜炎样本38个,腹膜炎样本中革兰阳性菌腹膜炎腹透液样本24个,革兰阴性菌腹膜炎腹透液样本14个。利用高效液相色谱法测定所有样本中降解的甘露糖、葡萄糖、岩藻糖浓度,比较无腹膜炎组腹透液样本与革兰阳性菌腹膜炎组、革兰阴性菌腹膜炎组腹透液样本在甘露糖、葡萄糖、岩藻糖水平上的差异;采用Logistic回归分析各单糖水平与腹膜炎发生的关系,采用受试者工作特征(ROC)曲线评估各单糖水平对腹膜炎不同病原菌的诊断价值。结果 3组各单糖水平差异有统计学意义(P<0.01)。与无腹膜炎组相比,腹膜炎组的葡萄糖浓度和岩藻糖浓度均显著下降(P<0.01)。与革兰阴性菌腹膜炎组相比,革兰阳性菌腹膜炎组甘露糖浓度显著下降(P<0.01)。Logistic回归分析表明...     

Hsa_circRNA_0017620 regulated cell progression of non‐small‐cell lung cancer via miR‐520a‐5p/KRT5 axis

BackgroundCircRNA is a very important functional RNA that plays an important role in the development and metabolism of cancer. However, the study of circRNA in NSCLC has not been fully elucidated.MethodsThe expression of hsa_circ_0017620, SFMBT2, miR‐520a‐5p, and KRT5 was determined using qRT‐PCR. KRT5, Twist1, E‐cadherin, and Ki67 protein expression were measured with western blot. The positive expression rates of Ki67 and Vimentin were determined by immunohistochemistry assay. 5‐Ethynyl‐2’‐deoxyuridine (EdU), colony formation, and MTT assays were used to assess cell proliferation. Transwell migration and invasion assay were applied to determine cell migration and invasion. Dual‐luciferase reporter and RNA immunoprecipitation assays were used to verify the relationship among hsa_circ_0017620, miR‐520a‐5p, and KRT5. The animal experiment was used to ensure the effects of hsa_circ_0017620 on tumor growth in vivo.ResultsHsa_circ_0017620 was upregulated in NSCLC cells and tissues. MiR‐520a‐5p had been verified to be a target miRNA of hsa_circ_0017620 and KRT5 had been verified to be a target mRNA of miR‐520a‐5p in NSCLC cells. Knockdown of hsa_circ_0017620 inhibited cell proliferation, migration, and invasion in NSCLC cells, which was reversed by downregulating miR‐520a‐5p or upregulating KRT5 in NSCLC. Overexpression of hsa_circ_0017620 had opposite effects in NSCLC. Moreover, hsa_circ_0017620 silencing inhibited tumor growth in vivo of NSCLC.ConclusionIn this study, we found that hsa_circ_0017620 played an important role in NSCLC progression. Hsa_circ_0017620 regulated cell proliferation, invasion, and migration through targeting miR‐520a‐5p/KRT5 axis in NSCLC, providing a potential new target for the treatment and diagnosis of NSCLC.     

结直肠癌患者外周血中癌胚抗原-mRNA的表达及其临床意义

目的：肿瘤复发转移仍是威胁患者生存的重要原因，及早检出外周血中的循环肿瘤细胞并进行有效的综合治疗有着重要的临床意义。检测结“直肠癌患者外周血中癌胚抗原（CEA）－mRNA的表达及血清CEA蛋白的含量，并分析其临床价值。方法：35例有明确临床肿瘤病灶存在的结直肠癌患者、9例根治术后患者和37例健康志愿者，分别以逆转录PCR（RT－PCR）检测其外周血中CEA－mRNA的表达，时间分辨荧光方法测定其血清CEA蛋白的含量。结果：35例结直肠癌患者CEA－mRNA阳性率为45．7％，而健康对照组阳性率为2．7％，两者比较差异有显著性（P＜0．05）；9例术后患者CEA－mRNA仅2例阳性；CEA－mRNA阳性表达率与肿瘤分期相关，与肿瘤细胞分化程度并不相关。CEA－mRNA阳性组血清CEA蛋白阳性率（72．2％）显著高于CEA－mRNA阴性组（42．3％）。结论：外周血细胞中CEA－mRNA的表达与肿瘤分期相关，有可能作为结直肠癌微转换的一个监测指标。长期的随访有助于评价其临床价值。     

重组人促红细胞生成素治疗结肠和直肠癌化疗相关贫血

目的 :观察重组人促红细胞生成素 (rhEPO)治疗结、直肠癌化疗相关贫血的疗效。方法 :选择 6 2例结、直肠癌采用以奥沙利铂为主联合方案化疗所致贫血患者 ,随机分为两组 :rhEPO治疗组 34例 ,给予皮下注射rhEPO 4 0 0 0 0U/周。对照组 2 8例 ,不给予rhEPO治疗 ,仅给予五参芪口服液。结果 :治疗后 4周起治疗组患者血红蛋白 (Hb) ,红细胞压积 (HCT)、红细胞总数 (RBC)均明显上升 ,与对照组相比差异有统计学意义 (P <0 .0 1)。rhEPO治疗组总有效率为 79.4 % ,对照组为 2 1.7% ,差异有统计学意义 (P <0 .0 1)。结论 :rhEPO 4 0 0 0 0U/周对晚期结、直肠癌联合方案化疗相关贫血有肯定的疗效     

慢性肝病中抗肝抗原自身抗体的检测

目的　探讨中国人不同病因所致慢性肝病患者中抗肝抗原自身抗体的存在状况及自身免疫性肝病的自身抗体特征。方法　 166例肝功能异常患者分为 6组 :自身免疫性肝炎 (AIH ) 12例、原发性胆汁性肝硬化 (PBC) 2 0例、原发性硬化性胆管炎 (PSC)13例、HBV组 66例、HCV组 2 2例、肝豆状核变性 (HDL) 3 9例。用间接免疫荧光法检测抗核抗体 (ANA)、平滑肌抗体 (SMA)、抗肝肾微粒抗体I型抗体 (anti LKM1)、抗线粒体抗体 (AMA)和抗中性粒细胞胞浆抗体 (ANCA) ,免疫印迹法检测抗肝细胞胞溶质抗原 1型抗体 (anti_LC1)、抗可溶性肝抗原 /肝胰抗原抗体 (anti_SLA/LP)、抗肝肾微粒抗体 1型 (anti_LKM1)、AMA_M2亚型等多种肝抗原自身抗体。结果　 166例中ANA、AMA、M_2、pANCA阳性率在 7组中有显著差异 (P <0 .0 1)。PBC中AMA、M 2阳性检出率均为 10 0 % ,PSC中pANCA阳性检出率为 5 3 8% ,Fisher精确检验在a =0 .0 0 2水准与其他各组比较有显著差异。AIH与PBC的ANA阳性率分别为10 0 %和 60 % ,Fisher精确检验在a =0 .0 0 2水准二者无显著差异。与其他各组比较有显著差异。在AIH组SMA阳性率为 2 5 % ,LKM 1、LC 1、SLA/LP阳性率均为 8.3 % ,统计学处理与其他组无显著差异 (P >0 0 5 ) ,可能与病例少有关。PBC中分别有 1     

Optical properties of human normal small intestine tissue determined by Kubelka-Munk method in vitro

AIM: To study the optical properties of human normal small intestine tissue at 476.5 nm, 488 nm, 496.5 nm, 514.5 nm, 532 nm, 808 nm wavelengths of laser irradiation. METHODS: A double-integrating-sphere system, the basic principle of measuring technology of light radiation, and an optical model of biological tissues were used in the study. RESULTS: The results of measurement showed that there were no significant differences in the absorption coefficients of human normal small intestine tissue at 476.5 nm, 488 nm, 496.5 nm laser in the Kubelka-Munk two-flux model (P>0.05). The absorption coefficients of the tissue at 514.5 nm, 532 nm, 808 nm laser irradiation were obviously increased with the decrease of these wavelengths. The scattering coefficients of the tissue at 476.5 nm, 488 nm, 496.5 nm laser irradiation were increased with the decrease of these wavelengths. The scattering coefficients at 496.5 nm, 514.5 nm, 532 nm laser irradiation were obviously increased with the increase of these wavelengths. The scattering coefficient of the tissue at 532 nm laser irradiation was bigger than that at 808 nm. There were no significant differences in the total attenuation coefficient of the tissue at 476.5 nm and 488 nm laser irradiation (P>0.05). The total attenuation coefficient of the tissue at 488 nm, 496.5 nm, 514.5 nm, 532 nm, 808 nm laser irradiation was obviously increased with the decrease of these wavelengths, and their effective attenuation coefficient revealed the same trend. There were no significant differences among the forward scattered photon fluxe, backward scattered photon fluxe, and total scattered photon fluxe of the tissue at 476.5 nm, 488 nm, 496.5 nm laser irradiation. They were all obviously increased with attenuation of tissue thickness. The attenuations of forward and backward scattered photon fluxes, and the total scattered photon fluxe of the tissue at 514.5 nm laser irradiation were slower than those at 476.5 nm, 488 nm, 496.5 nm laser irradiation respectively. The attenuations of forward and backward scattered photon fluxes, and total scattered photon fluxes at 532 nm laser irradiation were obviously slower than those at 476.5 nm, 488 nm, 496.5 nm, 514.5 nm laser irradiation. The attenuations of forward and backward scattered photon fluxes, and total scattered photon fluxe at 808 nm laser irradiation were all obviously slower than those at 476.5 nm, 488 nm, 496.5 nm, 514.5 nm, 532 nm laser irradiation respectively. CONCLUSION: There are significant differences in optical parameters of human normal small intestine tissue in the Kubelka-Munk two-flux model at six different wavelengths of laser radiation. The results would provide a new method of information analysis for clinical diagnosis.     

C反应蛋白与动脉粥样硬化性心脑血管病

在动脉粥样硬化形成的炎症机制中 ,C反应蛋白 (CRP)起着重要作用。超敏CRP检测可用于评价动脉粥样硬化性心脑血管病的危险性。通过监测CRP指导抗炎治疗 ,可能会在心脑血管病的一、二级预防中发挥重要作用。     

不同病理类型原发性甲状旁腺功能亢进症临床表现的比较分析

目的分析不同病理类型甲状旁腺功能亢进(PHPT)的临床表现特点。方法回顾性分析1958—2005年北京协和医院收治经手术病理证实的280例PHPT患者的临床资料。按病理类型分为甲状旁腺腺瘤组208例,甲状旁腺增生组52例及甲状旁腺腺癌组20例。结果腺癌组中男性高于另外2组(P<0.05)。增生及腺癌组中骨畸形、骨软化比例较低,增生组骨吸收、病理性骨折比例低于腺瘤组;胃肠道症状、多饮多尿及泌尿系统病变在腺癌组中高于另外2组(P均<0.05)。血总钙(TCa)、血游离钙离子(ICa)及24h尿Ca在腺癌组显著高于腺瘤组及增生组(P均<0.01),在腺瘤组与增生组间差异无统计学意义(P>0.05)。出现高钙危象的比例在腺癌组显著高于另外2组(P<0.01)。腺癌组病灶重量高于增生组,腺瘤组病灶直径小于增生组(P均<0.05)。结论在本组病例中,腺癌组男性比例较高,泌尿系统病变更为多见,出现高钙危象的比例显著增高,术后复发的比例较高。增生组骨骼系统病变相对较轻,其病变甲状旁腺重量低于腺癌与腺瘤组。