海马-杏仁核电镜改变与颞叶癫痫的关系

为研究海马 杏仁核的超微结构改变与颞叶癫痫的关系 ,对 35例颞叶癫痫病人行海马 杏仁核切除术 ,并对海马 杏仁核组织进行超微结构分析。痫灶区海马神经元以神经细胞固缩、变性为著 ,并可见有髓纤维的松解和粘连。轴索萌发出的新突出物、微管及微丝在无髓纤维上不规则排列和缠绕。神经毡内突触前终末兴奋性递质小泡增多 ,嵴消失 ,粗面内质网扩张 ,多聚核糖体解聚 ,并可见大量脂褐素及脂滴 ,星形细胞轻度水肿。提示颞叶癫痫发作不仅仅是单纯的生理生化改变所引起暂时性脑功能失调 ,其病理改变在颞叶癫痫的形成和持续状态具有重要的作用     

云南白药外敷治疗静脉穿刺致皮下淤血

目的 探讨云南白药外敷治疗静脉穿刺致皮下淤血的效果.方法 将94例静脉穿刺后引起皮下淤血的患者随机分成观察组(48例)和对照组(46例),观察组采用乙醇调制的云南白药外敷,对照组进行热敷治疗,观察患者淤血消退时间.结果 观察组淤血消退时间显著短于对照组(P＜0.01).结论 云南白药外敷治疗静脉穿刺引起的皮下淤血效果优于传统方法.     

Fungal adhesion protein guides community behaviors and autoinduction in a paracrine manner

Microbes live mostly in a social community rather than in a planktonic state. Such communities have complex spatiotemporal patterns that require intercellular communication to coordinate gene expression. Here, we demonstrate that Cryptococcus neoformans, a model eukaryotic pathogen, responds to an extracellular signal in constructing its colony morphology. The signal that directs this community behavior is not a molecule of low molecular weight like pheromones or quorum-sensing molecules but a secreted protein. Znf2, a master regulator of morphogenesis in Cryptococcus, is necessary and sufficient for the production of this signal protein. Cfl1, a prominent Znf2-downstream adhesion protein (adhesin), was identified to be responsible for the paracrine communication. Consistent with its role in communication, Cfl1 is highly induced during mating colony differentiation, and some of the Cfl1 proteins undergo shedding and are released from the cell wall. The released Cfl1 is enriched in the extracellular matrix and acts as an autoinduction signal to stimulate neighboring cells to phenocopy Cfl1-expressing cells via the filamentation-signaling pathway. We further demonstrate the importance of an unannotated and yet conserved domain in Cfl1’s signaling activity. Although adhesion proteins have long been considered to be mediators of microbial pathogenicity and the structural components of biofilms, our work presented here provides the direct evidence supporting the signaling activation by microbial adhesion/matrix proteins.     

Warm currents affecting the spring and winter distributions of living coccolithophores in the Yellow Sea,China

Spring and winter distributions of living coccolithophores in the Yellow Sea were studied using a polarizing microscope based on two surveys in April 2010 and January 2011. Nine species were recorded, including Emiliania huxleyi and Gephyrocapsa oceanica as the predominant forms. The cell abundance of all coccolithophores in the spring was significantly higher than in the winter (p<0.01), with means of 15 387 and 2470 cells l-1, respectively. Notably, we observed a habitat selection of coccolithophores in the southeastern Yellow Sea (32–35°N, 123–125°E). This habitat was characterized by sharp temperature gradients during both seasons caused by water exchange between the shelf waters and the Yellow Sea Warm Current. Moreover, the cell abundance, standing crop and estimated fluxes of coccolithophores were abnormally high compared to other regions. The habitat preference of coccolithophores in the Yellow Sea was directly related to the hydrodynamic conditions. The unfavorable light conditions caused by the intense convective mixing were responsible for the low biomass of coccolithophores during the winter. However, the increasing temperature influenced by the warm current, in combination with water column stability, triggered the spring bloom of coccolithophores in the southern Yellow Sea.     

电脑控温毯对重症手足口病患儿的控温效果

目的探讨电脑控温毯对重症手足口病患儿体温的控制效果。方法将2011年6月至2012年1月儿科重症监护室收治的68例重症手足口病患儿按照随机数字表法分为研究组和对照组各34例,对照组在体温〉39℃时使用布洛芬混悬滴剂降温;研究组在体温〉39℃时使用布洛芬混悬滴剂联合电脑控温毯降温。观察两组患儿在采用降温措施后1、2、3、4、6h的体温变化及全天呼吸、心率的波动幅度,高热持续时间。结果研究组的降温效果明显优于对照组（P〈0．01）;研究组全天呼吸、心率波动幅度明显小于对照组（P〈0．01）,高热持续时间较对照组明显缩短（P〈0．01）。结论使用电脑控温毯能有效控制重症手足口病患儿的体温,缩短病程,有效防止并发症。     

中医药防治传染病的体系建设探讨

传染病起病急、传变快、波及范围广的致病特点需要中医药的防治工作要建立在体系建设的基础上。目前我国新建的中医药防治传染病的体系建设已初见成效,本文通过分析当前该体系建设所采取的策略和措施、已取得的成果以及存在的问题,为推进中医药防治传染病体系规范化可持续建设提供参考。     

基于代谢组学的针灸作用机制研究进展

代谢组学是新近兴起的一种系统生物学研究手段,具有整体性、动态性反应生物体接受刺激时所作出的代谢物层面的变化,与中医整体观十分吻合,因此广泛用于中医中药学的研究中,在针灸学机制的研究中早已将代谢组学作为一种有效的研究手段,近年来针灸代谢组学的研究日趋增多,在揭示针灸作用机制方面也取得了一定的成果,故对现今的研究做一综述,以愿能为代谢组学广泛应用于针灸的现代化研究提供新思路。     

胃肠舒片的质量标准

目的：建立胃肠舒片的质量标准。方法：采用TLC法对制剂中组成药味大黄、枳壳、甘草进行鉴别;用HPLC法测定胃肠舒片中柚皮苷的含量,使用Thermo BDS色谱柱（250 mm×4.6 mm,5μm）,流动相：乙腈-水溶液（17∶83）;检测波长：230nm;流速：1.0 ml·min-1;柱温：室温;进样量：20μl。结果：TLC法能对制剂中的大黄、枳壳、甘草进行专属性定性鉴别;高效液相色谱法中柚皮苷对照品在125.00～4000.00 ng·ml-1浓度范围内线性关系良好（r=0.999 3）,回收率为98.49%（RSD=1.39%,n=6）。结论：该质量控制方法简便、可靠、准确,可用于胃肠舒片的质量控制。     

成都市两城区部分成年男性吸烟者吸烟行为调查

目的 分析成都市成年男性吸烟者吸烟行为、吸烟量及其变化的影响因素。方法对成都市两城区320名成年男性吸烟者面对面问卷调查。采用描述性分析、t检验、χ²检验、单因素方差分析、Kruskal-Wails H秩和检验、累积比数logistic回归方法分析数据。结果 分析显示吸烟者更换卷烟后, 吸烟量增加(t=2.327, P=0.021), 焦油量降低(t=-11.251, P<0.001);累积比数logistic回归分析显示, 学历较低(OR=1.968, P=0.040)、收入较高(OR=2.053, P=0.043)、烟蒂较短(OR=2.366, P=0.010)、尼古丁高依赖(OR=7.143, P<0.001)者吸烟量更大。结论 更换卷烟的吸烟者更倾向选择低焦油卷烟;学历低、收入高、尼古丁高依赖的吸烟者是控烟健康教育、行为干预的重点人群。     

急性主动脉夹层患者首发症状与院前延迟的关系研究

目的 探讨急性主动脉夹层患者首发症状与院前延迟的关系,为临床实施院前针对性健康教育提供参考.方法 选取急性主动脉夹层患者488例,收集患者首发症状、一般资料、疾病相关因素、院前相关因素及院前时间,分析首发症状与院前延迟的关系.结果 患者院前时间为135.5(48.3,1735.5) min,其中院前时间≤150 min...