足反射疗法对前列腺增生效果分析

目的　前列腺增生是男性中老年的常见病、多发病。单纯药物治疗效果不明显 ,手术治疗会给患者造成痛苦和不便。了解足反射疗法对此病的疗效。方法　对 90例前列腺增生患者进行了分组对比 ,A组 3 0例 ,采用足反射疗法。B组 3 0例 ,采用药物疗法。C组 3 0例 ,采用足反射疗法与口服药物双重治疗。三组均以B超声诊断仪测定。结果　治疗前与第一疗程后 ,前列腺三径之差进行比较。显效 :两次差≥ 1 5cm。有效二两次差≥ 1 0cm。结果 :A组显效率 3 6 60 % ,有效率 5 3 3 3 % ,总有效率 89 99%。B组 :显效率 3 3 3 %。有效率 10 % ,总有效率 13 3 3 %。C组 :显效率 5 6 66%、有效率 3 6 66% ,总有效率 93 3 2 %。结论　证实了足反射疗法可作为治疗前列腺增生及前列腺炎的首选方法。如与药物疗法配合使用 ,效果更好 ,并可解除有手术禁忌患者的痛苦。     

RT—PCR方法在小鼠肝炎病毒检测中的初步应用

采用4株小鼠肝炎病毒,MHV1、MHV3、A59和JHM的混合液人工感染SCID小鼠,接种病毒后1、2、5d分别取小鼠的全血、肝、肺、脑、脾和血清,用组织总RNA抽提试剂盒提取各组织的总RNA,然后用RTPCR方法检测小鼠肝炎病毒,并对各反应产物进行平均光密度测定。结果显示,接种后24h即可检测出血液中的小鼠肝炎病毒,病毒在小鼠体内各脏器出现的先后顺序为血、肝、脾、肺、脑,主要在肝脏进行复制。     

美国药品召回制度对我国药品安全的启示

目的 :为我国建立药品召回制度提供参考。方法 :介绍美国药品召回制度 ,探讨其对我国药品安全的启示。结果与结论 :借鉴美国的药品召回制度 ,完善我国的法律规范体系 ,全面推行药品召回制度。     

高效液相色谱法同时测定黄连解毒胶囊中5组分的含量

目的:建立以高效液相色谱法同时测定黄连解毒胶囊中盐酸小檗碱、盐酸巴马亭、盐酸药根碱、黄芩苷、栀子苷含量的方法。方法:采用3种紫外检测波长同时进行测定,色谱柱为DiamonsilC18,流动相为水-甲醇-0.05%磷酸(梯度洗脱),柱温为35℃,流速为1.0ml/min,检测波长为345、280、238nm,进样量为10μl。结果:盐酸小檗碱、盐酸巴马亭、盐酸药根碱、黄芩苷、栀子苷进样量分别在0.105μg～1.680μg(r=0.9999)、0.045μg～0.720μg(r=0.9998)、0.065μg～1.040μg(r=0.9997)、0.190μg～3.040μg(r=0.9999)、0.145μg～2.320μg(r=0.9999)范围内呈良好的线性关系;平均加样回收率分别为99.11%、98.19%、97.21%、98.52%、99.22%。结论:本方法快速、重现性好、灵敏度高,可为黄连解毒胶囊提供更合理、可靠的质量控制方法。     

Imidapril treatment improves the attenuated inotropic and intracellular calcium responses to ATP in heart failure due to myocardial infarction

1. Adenosine 5'-triphosphate (ATP) is known to augment cardiac contractile activity and cause an increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) in isolated cardiomyocytes. However, no information regarding the ATP-mediated signal transduction in the myocardium in congestive heart failure (CHF) is available. 2. CHF due to myocardial infarction (MI) in rats was induced by the occlusion of the left coronary artery for 8 weeks. The positive inotropy due to ATP was depressed in failing hearts. Treatment of 3 weeks infarcted animals with imidapril (1 mg kg(-1) day(-1)) for a period of 5 weeks improved the left ventricle function and decreased the attenuation of inotropic response to ATP. 3. ATP-induced increase in [Ca(2+)](i) was significantly depressed in cardiomyocytes isolated from the failing heart and this change was partially attenuated by imidapril treatment. However, the binding characteristics of (35)S-labeled adenosine 5'-(gamma-thio) triphosphate in sarcolemma isolated from the failing heart remained unaltered. 4. ATP-induced increase in [Ca(2+)](i) was depressed by verapamil and cibacron blue in both control and failing heart cardiomyocytes; however, the ATP response in the failing hearts, unlike the control preparations, was not decreased by ryanodine. This insensitivity to ryanodine was attenuated by imidapril treatment. 5. Treatment of infarcted rats with enalapril and losartan produced effects similar to imidapril. 6. These findings indicate that the positive inotropic response to ATP and ATP-induced increase in [Ca(2+)](i) in cardiomyocytes are impaired in heart failure. Furthermore, blockade of renin angiotensin system prevented the impairment of the ATP-mediated inotropic and [Ca(2+)](i) responses in the failing heart.     

Study on chemical constituents of Folium Artemisiae argyi Carbonisatum,toxicity evaluation on zebrafish and intestinal hemostasis

Folium Artemisiae argyi Carbonisatum (FAAC) is a traditional medicine widely used in clinic. It has the effect of hemostasis by warming meridians. In order to further explore the chemical composition and biological activity of FAAC, the methanol extract of FAAC was isolated and purified by open column and high- performance liquid chromatography. and the complete structure was characterized by nuclear magnetic resonance (NMR) and LREI-MS for the first time, namely rutin, quercetin and octacosanol respectively. Initially the toxic effect of methanol extract of FAAC on zebrafish was evaluated by observing the phenotypic characteristics, spontaneous twitch times, heart rate, hatching rate, the distance of SV-BA and cardiomyocyte apoptosis of zebrafish. The results showed that FAAC has embryonic development toxicity and cardiotoxicity when it was higher than 62.5 μg/mL. Meanwhile, the hemostatic effect of methanol extract of FAAC was compared with FAA (Folium Artemisia argyi) by zebrafish intestinal bleeding model originally. The results showed that the hemostatic effect of the medium and high concentration dose groups (3.0 and 30.0 μg/mL) was enhanced for both FAAC and FAA. This study provided an experimental basis for the clinical application of FAAC.     

关于优良药房工作规范的探讨

优良药房工作规范(Good Pharanacy Practice,GPP)是药师在药房中实施“药学服务”(Pharmaceutical care)的行为准则,是衡量药师为病人或消费者服务质量的标准,也是药房自觉按照科学化、规范化和人本化管理的过程。     

《保健食品注册管理办法(试行)》的进步与不足

2005年6月2日,国家食品药品监督管理局以局令第19号的形式发布了《保健食品注册管理办法（试行）》（以下简称《注册办法》）,全文共九章,一百零五条,对涉及保健食品注册和审批的各个环节进行了界定.该办法于2005年7月1日起施行.本文试图对《注册办法》进行一些分析,在立法理念和立法技术层面上谈谈自己的一些粗浅看法。     

2003～2004年南昌市泌尿生殖道支原体的耐药分析

目的了解南昌地区STD门诊患者泌尿生殖道支原体感染及耐药情况，为临床治疗提供参考依据。方法应用法国生物梅里埃公司的IST2试剂盒进行支原体培养＋药敏检测：结果原始霉素（95.5％）、交沙霉素（91．8％）、强儿霉素（90％）、四环素（81．9％）对Uu的敏感性很强，其次是克拉毒素（63．9％）。阿奇毒素为常用抗生素，但敏感半仅为（42．4％），而氧氟沙星（10．8％）和环丙沙星（1．8％）对Uu的敏感性相对较低：结论原始霉素、交沙霉素、强力霉素、四环素等应作为本地区目前治疗泌尿生殖道支原体感染的首选抗生素。     

浅论中医学术流派研究与发扬

目的 中医药学作为一项复杂的系统工程,其创新发展离不开高质量的传承、吸收和充实.因而,学术流派的创新和发展就显得尤为重要.创新发展学术流派,应着眼传承,立足组织机构,以发展传承为抓手,以建立健全组织机构为载体,在传承和组织成体两个大方面的创新发展中提升学术流派的发展质量.