Correction to: Triadic Interactions in MIECHV: Relations to Home Visit Quality

Maternal and Child Health Journal - The article “Triadic interactions in MIECHV: Relations to home visit quality”, written by Carla A. Peterson, Kere Hughes-Belding, Neil Rowe, Liuran...     

Susceptibility of Enterococcus faecalis biofilm to antibiotics and calcium hydroxide

The purpose of this study was to investigate the antimicrobial efficacy of six groups of antibiotics and calcium hydroxide against Enterococcus faecalis biofilm in a membrane filter model. Two-day-old E. faecalis (ATCC 29212) biofilm was exposed to ampicillin, co-trimoxazole, erythr omycin, oxytetracycline, vancomycin, vancomycin followed by gentamicin, Ca(OH)(2), and phosphate-buffered saline (control). After 1 h of exposure, the antimicrobial activity was neutralized by washing each disc five times in PBS, and then the colony-forming units of the remaining viable bacteria on each disc were counted. The results revealed that only erythromycin, oxytetracycline and Ca(OH)2 showed 100% biofilm kill. An ANOVA with a Bonferroni post hoc test (P < 0.05) detected significant differences among the test agents, except in the ampicillin group versus the co-trimoxazole group. It is concluded that erythromycin, oxytetracycline and Ca(OH)2 are 100% effective in eliminating E. faecalis biofilm, whereas ampicillin, co-trimoxazole, vancomycin, and vancomycin followed by gentamicin are ineffective.     

Rapid carrier screening for beta-thalassemia by single-step allele-specific PCR and detection

OBJECTIVES: This study aimed to evaluate a rapid molecular carrier screening strategy for beta-thalassemia. DESIGN AND METHODS: Allele-specific PCR was combined with amplicon detection by dissociation curve analysis of SYBR Green I fluorescence in a single step. RESULTS: The presence of a particular mutation results in the amplification of a mutation-specific product and the dissociation temperature of each amplicon was highly reproducible. CONCLUSIONS: Homogeneous allele-specific PCR amplification and detection of multiple beta-globin mutations can serve as a rapid and inexpensive carrier screening tool.     

呼气末一氧化碳与新生儿疾病

[摘要]一直以来,一氧化碳(carbon monoxide, CO)被认为是代谢废物和毒性气体,导致环境污染及人类死亡。 直至1949年,Sjostrand T首次报道,机体降解血红素产生胆红素IXa的同时会产生等当量的 CO,内源性 CO 受到关注,许多学者致力于内源性 CO 的产生、代谢、生物学效应、检测方法等研究,内源性CO作为一项检测指标逐渐被应用于临床。随着检测技术的发展,用于评价内源性CO产生水平的矫正室内一氧化碳的呼气末一氧化碳值 (ETCOc)作为一 种无创性检查方法也开始应用于临床。 新生儿作为一个特殊群体,许多常规的检查方法应用于新生儿存在潜在很多弊端,不易被家属接受,存在延误诊疗的风险。因此,寻找一种能用于早期诊断新生儿常见疾病,并持续评估患儿病情动态变化的无创性检查方法具有重要意义。     

Effectiveness and acceptability of a multidisciplinary approach in improving the care of patients with advanced chronic kidney disease: a pilot study

International Urology and Nephrology - This study aimed at determining the feasibility of conducting a large-scale pragmatic effectiveness study on the implementation of multidisciplinary care...     

Abnormal hippocampal substructure volume in insomnia disorder

Brain Imaging and Behavior - To date, our understanding of the role of abnormal hippocampal volume in imaging studies of insomnia disorders (ID) has remained in apparent contradiction. Given that...     

17例永存左上腔静脉畸形的超声检查与手术对比分析

对１７例经手术证实的先心病合并永存左上腔静脉畸形（ＬＳＶＣ）的患者进行回顾性对比分析，研究超声心动图诊断ＬＳＶＣ的敏感性并探讨提高其检出率的方法。发现在１７例已证实合并ＬＳＶＣ的多种先心病患者中，术前经胸超声心动图检出ＬＳＶＣ１２例，占７１％，胸骨上窝探查有特异表现。后回顾复习患者资料，作者认为多切面的仔细探查，绝大多数患者可用经胸超声心动图在术前检出，可为外科手术提供更多的信息，以指导手术