音乐疗法联合右美托咪定对LC患者炎症的影响

目的：探讨音乐疗法联合右美托咪定对腹腔镜胆囊切除术(LC)患者炎症的影响。方法：选择2020年10月～2021年4月本院因胆囊良性疾病全身麻醉下择期行LC手术患者60例,随机分为两组,每组30例：对照组给予右美托咪定治疗;试验组给予音乐疗法+右美托咪定治疗。记录两组术中情况和生命体征;采用视觉模拟评分法(VAS)评估患者术后疼痛程度;用ELISA法测定血浆中TNF-α、IL-8、IL-10的浓度。结果：两组患者的ASA分级、性别、年龄、体重指数、麻醉时长、手术时长、输液量、出血量比较差异均无统计学意义。试验组在出手术室时以及术后8h、24h、48h的VSA评分与对照组同时间点相比较均明显降低。接受音乐疗法后,试验组的MAP、HR与对照组同时间点相比较均明显降低;试验组TNF-α、IL-8的浓度与对照组同时间点相比较均明显降低;试验组IL-10的浓度与对照组同时间点相比较明显升高。结论：音乐疗法联合右美托咪定可以更好的缓解LC患者术后疼痛,降低炎症反应。     

Siglec-1与原发性胆汁性肝硬化的相关性研究

目的 检测Siglec-1(sialic acid-binding immunoglobulin-like lectins,唾液酸结合的免疫球蛋白样凝集素,CD169)在原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)患者外周血单核细胞上的蛋白及mRNA表达水平,并探讨其在原发性胆汁性肝硬化发生发展中的作用.方法 流式细胞术检测45例PBC患者及36例健康对照者、40例肝炎后肝硬化对照者外周血CD14,CD169双阳性细胞的表达率;实时荧光相对定量RT-PCR方法检测入选对象单核细胞中Siglec-1mRNA的含量;生化常规测定所有入选者血清生化指标水平.结果 流式细胞术检测结果显示:PBC组单核细胞CD14,CD169双阳性率为13.0%±2.5%,显著高于健康对照组(1.0%±0.2%,P<0.01)及肝炎后肝硬化对照组(4.1%±0.5%,P<0.01).PBC组Siglec-1mRNA的相对表达量为健康对照组的3.42倍,差异具有统计学意义(P<0.01).结论 PBC患者单核细胞表面siglec-1蛋白表达显著增高,mRNA含量显著增加,说明PBC患者外周血单核细胞已经发生巨噬细胞化,单核巨噬细胞介导的免疫炎症反应在PBC发生发展过程中起重要作用.     

弥可保联合空气压力波治疗糖尿病周围神经病变临床分析

目的探讨弥可保和空气波压力治疗仪联合治疗糖尿病周围神经病变的效果。方法将96例糖尿病周围神经病变患者随机分成两组,对照组单独用弥可保治疗,治疗组弥可保联合空气波压力治疗仪治疗,观察治疗前后自觉症状、腱反射和神经传导速度。结果治疗组总有效率为91．67％,对照组为70．83％,神经传导速度较对照组有显著改善,两组差异有统计学意义（P〈0．05）。结论弥可保联合空气波压力治疗仪治疗糖尿病周围神经病变疗效显著,值得临床推广。     

拉玛泽减痛分娩法对分娩影响的研究

目的观察拉玛泽减痛分娩法对分娩的影响,探讨其训练效果的可行性,促进自然分娩。方法选择184例自愿参加拉玛泽减痛分娩法训练的孕妇为观察组,186例无参加拉玛泽减通分娩法训练的为对照组。比较两组产时疼痛程度,总产程,分娩结局。结果观察组总产程,产后出血,新生儿窒息与对照组相比有明显差异,而疼痛程度评价无差异。结论拉玛泽减痛分娩法能有效缩短总产程,减少产后出血,降低新生儿窒息率,降低社会因素剖宫产,保障母婴健康。     

中西医结合治疗开放性胫腓骨骨折的疗效及术后感染分析

目的分析中西医结合治疗开放性胫腓骨骨折的临床疗效及术后感染等并发症。方法选取开放性胫腓骨骨折患者164例,随机分为观察组和对照组各82例,观察组予中西医结合治疗,对照组仅予抗菌药物等西药治疗,比较两组患者骨痂生长情况、治疗优良率及并发症发生率。结果观察组术后10周内骨痂出现率为91.46%,对照组为67.07%,观察组患者术后10周内骨痂出现率明显高于对照组;观察组患者临床疗效优良率为92.68%,明显高于对照组的78.05%;观察组并发症总发生率为12.41%;明显高于对照组的25.61%,差异均有统计学意义(P<0.05)。结论中西医结合方法治疗开放性胫腓骨骨折,骨痂生长快,疗效优良率高,术后并发症少,较少出现术后感染。     

尿道下裂分型及与外生殖系统畸形的关系

目的 探讨尿道下裂分型及与合并外生殖系统畸形的相关性.方法 回顾性分析2000年1月至2009年10月收治的632例尿道下裂患者资料,分别统计各型尿道下裂的比例及合并外生殖系统畸形的比例,采用精确概率法及Spearman秩相关分析进行统计学处理.结果 632例尿道下裂患者中,轻度尿道下裂80例(12.6%),中度144例(22.8%),重度273例(43.2%),特重度135例(21.4%).发生阴茎阴囊转位、隐睾、鞘膜积液、隐匿型阴茎、假阴道等外生殖系统畸形共184例次,合并畸形157例,占24.8%.尿道下裂分型与总体畸形、阴茎阴囊转位、隐睾的发生具有统计学意义,并呈正相关;而尿道下裂分型与鞘膜积液的发生无统计学意义.结论 尿道下裂患者以中度以上者占多数,以合并阴茎阴囊转位者最常见,其次为隐睾、鞘膜积液;尿道下裂越严重,合并外生殖系统畸形的概率及发生阴茎阴囊转位、隐睾的概率越高.

Abstract：

Objective To investigate the correlation of the type of hypospadias and external genital system malformations. Methods From Jan 2000 to Oct 2009, the data of 632 patients diagnosed with hypospadias was analyzed retrospectively. The proportion of all types of hypospadias and the frequency of external genital system malformations were statistically analyzed. Results Among all cases, 12.6% exhibited mild hypospadias, 22. 8% moderate, 43. 2% severe and 21.4% had extremely severe hypospadias. One hundred and eighty-four cases with external genital system malformations were classified into penoscrotal transposition, cryptorchidism, hydrocele of tunica vaginalis,concealed penis and pseudovagina. The probability was about 24.8% (157 cases) of 632 patients with hypospadias. There was significant difference and a positive correlation among the type of hypospadias with total malformations, penoscrotal transposition, cryptorchidism. Conclusions In this patient cohort, moderate and severe hypospadias accounted for the majority of hypospadias. Penoscrotal transposition was the most frequent external genital system malformation in hypospadias, followed by cryptorchidism and hydrocele of tunics vaginalis. More severe hypospadias is accompanied with a higher probability of the external genital system malformations, penoscrotal transposition and cryptorchidism.     

IL-37b gene transfer enhances the therapeutic efficacy of mesenchumal stromal cells in DSS-induced colitis mice

Aim:

To investigate whether the transfer of the IL-37b gene, a newly identified inhibitor of both innate and adaptive immunity, could improve the therapeutic efficacy of mesenchumal stromal cells (MSCs) in inflammatory bowel disease (IBD).

Methods:

The expression of IL-37 in biopsied specimens of the patients with active ulcerative colitis (UC) was detected using RT-PCR and immunohistochemistry. Mice were treated with 3% dextran sulfate sodium (DSS) for 8 days to induce colitis. Before DSS treatment, the mice were injected with MSCs, MSC-eGFP or MSC-IL37b. Their body weight was measured each day, and the colons and spleens were harvested on d 10 for pathological and biochemical analyses.

Results:

In biopsied specimens of the patients with active UC, the expression of IL-37 was dramatically elevated in inflamed mucosa, mainly in epithelial cells and infiltrating immune cells. Compared to MSC-eGFP or MSCs, MSC-IL37b administration significantly attenuated the body weight and colon length reduction, and decreased the histological score in DSS-induced colitis mice. Furthermore, MSC-IL37b administration increased the percentage of myeloid-derived suppressor cells (MDSCs) among total splenic mononuclear cells as well as the percentage of regulatory T cells (Tregs) among splenic CD4+ T cells in the mice. Moreover, MSC-IL37b administration increased the IL-2+ cells and decreased the IFN-γ+ cells among splenic CD4+ T cells.

Conclusion:

IL-37 is involved in the pathophysiology of UC. IL-37b gene transfer enhances the therapeutic efficacy of MSCs in DSS-induced colitis mice by inducing Tregs and MDSCs and regulating cytokine production.     

氢化物原子荧光光度法测定婴幼儿配方奶粉中总砷的不确定度评定

<正>原子荧光法测定微量砷具有灵敏度高,简便易行,重现性好的特点,已成为砷含量分析的首选方法。CNAS-CL07明确指出,当不确定度与检测结果的有效性或应用有关或用户有要求时,或当不确定度影响到对规范限度的符合性时,当测定方法中有规定时和CNAS有要求时,检测报告必须提供测量结果的不确定度。本文根据国家质量监督检验检     

Neuroprotective effect of interleukin-6 regulation of voltage-gated Na~+ channels of cortical neurons is time-and dose-dependent

Interleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorly understood. This study investigated the effects of 24 hour exposure of interleukin-6 on cortical neurons at various concentrations(0.1, 1, 5 and 10 ng/m L) and the effects of 10 ng/m L interleukin-6 exposure to cortical neurons for various durations(2, 4, 8, 24 and 48 hours) by studying voltage-gated Na+ channels using a patch-clamp technique. Voltage-clamp recording results demonstrated that interleukin-6 suppressed Na+ currents through its receptor in a time- and dose-dependent manner, but did not alter voltage-dependent activation and inactivation. Current-clamp recording results were consistent with voltage-clamp recording results. Interleukin-6 reduced the action potential amplitude of cortical neurons, but did not change the action potential threshold. The regulation of voltage-gated Na+ channels in rat cortical neurons by interleukin-6 is time- and dose-dependent.     

不同基牙设计对CAD/CAM全瓷修复体抗折强度的影响

目的 研究不同CAD/CAM全瓷修复体修复根充后前磨牙的抗折强度。方法 收集21颗因正畸拔除的上颌第一前磨牙,制备近面及舌尖缺损洞型。样品分为3组,第1组为直接树脂充填组;第2组为保留缺损组;第3组为纤维桩核组,然后利用IPS e.max CAD全瓷修复体进行修复,采用Rely X U200树脂黏结剂进行黏固。对所有样品进行抗折强度测试,采用光学显微镜观察样品的断裂类型。采用SPSS 15.0软件包对数据进行统计学分析。结果 纤维桩核组全瓷修复体的抗折强度显著大于树脂充填组和保留缺损组。第1组和第2组以修复体破裂为主,第3组除修复体破裂外,还发生牙体组织破裂。结论 对于伴有功能牙尖缺损的前磨牙,建议采用纤维桩核+全瓷冠进行修复。