雷公藤对雄性大鼠脑垂体前叶黄体生成细胞的影响

利用抗β-黄体生成素(β-LH)单克隆抗体,采用免疫组织化学方法,并结合电镜超微结构,观察雷公藤总甙(GTW)和单体T_4对雄性大鼠脑垂体前叶黄体生成细胞(LH细胞)的影响。结果表明,LH细胞出现一些形态改变。随着雷公藤剂量的增加,LH细胞胞浆内免疫酶反应增强,胞浆内出现空泡。超微结构也显示,LH细胞胞核皱缩,胞浆内高尔基复合体和内质网肿胀,在总甙组和T_4大剂量组,LH细胞类似“阉割细胞”样变。提示雷公藤的抗生育作用类似去性腺效应.     

HLA—D抗原的相容性以及封闭因子与习惯性流产的关系的实验研究

运用单向混合淋巴细胞培养的方法分别检测了10例习惯性流产史船娠夫妇间、流产史孕妇和无关第三者(男性)间及9例正常妊娠夫妇间的HLA-D抗原的相容性。结果发现流产史夫妇间的HLA-D抗原相容性大于流产史孕妇和无关第三者(男性)之间及正常夫妇间的HLA-D抗原相容性(P<0.01)。提示夫妇间的HLA-D抗原相容性增大可能是导致习惯性流产的原因之一。同时,在反应体系中分别加入不同孕妇血浆以观察其对单向混合淋巴细胞培养(单向MLC)的影响时发现,流产史孕妇血浆对单向MLC不仅无抑制作用,反而有刺激作用,而正常孕妇血浆对单向MLC有抑制作用。推测正常孕妇血浆中存在特异和/或非特异的封闭因子,而流产史孕妇血浆中缺乏特异和/或非特异的封闭因子。     

Characterization and distribution of [3H]ohmefentanyl binding sites in the human brain

Binding properties and localization of [3H]ohmefentanyl, a new ligand for mu opioid receptors, were investigated on normal human brain sections. Binding assays performed at the level of the basal ganglia revealed: (1) a steady-state binding reached after 60 min incubation at room temperature, (2) the presence, in saturation experiments, of an apparent single class of binding sites with a Kd = 1.68 +/- 0.45 nM and a Bmax = 162 +/- 9 fmol/mg protein, (3) an order of potency to inhibit [3H]ohmefentanyl binding as follows: ohmefentanyl greater than [D-Ala2, MePhe4, Gly-ol5] enkephalin (DAGO) greater than ethylketocyclazocine (EKC) much greater than Tyr-D-Ser(OtBu)-Gly-Phe-Leu-Thr(OtBu) (BUBU) and U-50,488H. Quantitative autoradiography showed an heterogeneous distribution of [3H]ohmefentanyl binding sites with the highest densities in amygdala, medical geniculate body, thalamus, and caudate nucleus. Binding characteristics and anatomical distribution also show that [3H]ohmefentanyl may bind to a small proportion of additional sites called "DAGO-inaccessible [3H]ohmefentanyl specific binding sites." [3H]Ohmefentanyl binding to these sites can be partly inhibited by sigma ligands such as 1,3-di-o-tolylguanidine (DTG) and haloperidol. However, unlabeled DAGO inhibited more than 80% of [3H]ohmefentanyl specific binding in most of the human brain regions studied, suggesting that the major population of sites labeled by [3H]ohmefentanyl represented mu opioid receptors.     

14例大疱性表皮坏死松解型药疹治疗体会

报告14例大疱性表皮坏死松解型药疹的治疗。以磺胺类药和解热镇痛药引起者居多,各4例。皮损面积按九分法计算,皮损面积80％以上者9例,80％以上者5例,治愈9例,死亡5例,我们认为早期使用足够剂量皮质激素类药物是有效措施。病程中注意防治感染,给予足够的液体以防止水电解质失平衡,局部治疗使用本院中药“五黄油”。     

Effect of cimetidine on wedged hepatic venous pressure in cirrhotic patients

According to our supposition that "humoral mechanism" plays an important role in the pathogenesis of portal hypertension due to cirrhosis, antagonists to some of humoral substances would lower the portal pressure in cirrhotic patients. Wedged hepatic venous pressure (WHVP) was used as an indicator for changes of portal pressure. Cimetidine was given intravenously to 8 cirrhotic patients, in whom an average lowering of 0.72 kPa (7.3 cm H2O) of WHVP was observed subsequently. This change was of clinical significance as compared with the previous results of splenorenal shunting operations.

     

矿泉“851”对抗疟治疗的非特异性免疫作用

感染伯氏疟原虫(P.berghe ANKA株)小鼠,经矿泉“851”及与抗疟酮合用治疗后,腹腔巨噬细胞吞噬百分率和吞噬指数明显高于感染对照组(P<0.01)。两组药均能提高小鼠单核巨噬细胞系统(MPS)对碳微粒的廓清率,其K值与对照组相比,P<0.01,MPS活性的增强程度与其疟原虫的抑制率成正相关。     

中药黄藤中的季铵生物碱研究

本文从中药黄藤的根及根茎中分得三种季铵生物碱,化学及光谱数据证明,一为掌叶防己碱(巴马汀),二为药根碱,三系伪非洲防己胺碱。后者为国内首次得到的一种天然伪原小檗碱型生物碱。