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101.
目的:探讨内皮细胞清除补体攻膜复合物(MAC)的途径及其清除动力学,方法:原代培养的人脐静脉内皮细胞以RH414荧光标记质膜双层,0℃组装亚溶剂量的MAC,37℃复苏后,LSCM实时监MAC沉积诱导的质膜囊泡化形成和胞吞,胞吐情况,流式细胞仪定量检测内皮细胞表面MAC抗原的清除情况,结果:MAC沉积后,内皮细胞有的质膜囊泡化形成,囊泡以胞吞和胞吐2种方式离开细胞,并以前者占优,37度条件下,内皮细胞清除表面MAC的半衰期约为5min。结论:内皮细胞可通过胞吞和胞吐2种机制清除细胞表面沉积的MAC,并以胞吞方式为主。  相似文献   
102.
103.
目的总结桡骨远端粉碎性骨折的外固定架治疗疗效。方法从1995年~2003年采用外固定架治疗桡骨远端粉碎性骨折46例,并进行随访。结果治疗后均随访1年,术后按Dienst评分,优良率为83.3%。结论外固定架固定牢靠,可早期活动腕关节,是治疗桡骨远端粉碎性、开放性骨折的有效方法。  相似文献   
104.
A veronal buffer extract of Salmonella typhi was used as the reference antigen and its corresponding rabbit antiserum as the reference antibody in crossed immunoelectrophoresis to analyze antibodies in sera obtained from typhoid patients and carriers. Four precipitating antibodies were regularly detected. Three were against antigens common to other gram-negative bacteria and one appeared to be typhoid specific. Of the three common antigens, one (antigen no. 7) formed a precipitin resembling in mobility and morphology the lipopolysaccharide antigen seen in crossed immunoelectrophoresis analysis of other gram-negative bacteria. The other (antigen no. 19) was heat labile and antigenically similar to the reported common antigen of Pseudomonas aeruginosa. The third (antigen no. 14), also heat labile, was present in members of the family Enterobacteriaceae but not the family Pseudomonas. The typhoid-specific precipitating antibody present in sera of most typhoid patients and carriers but not patients infected with nontyphoid salmonella was directed to a heat-labile, non-O, non-H, and non-Vi antigen (antigen no. 28), probably protein in nature.  相似文献   
105.
腺病毒载体介导的lacZ基因在NG细胞系及大鼠黑质的表达   总被引:1,自引:0,他引:1  
本实验用标记基因lacZ5型重组腺病毒(Ad5CMVlacZ)转染培养的NG细胞系,X-gal染色检测转染效率.在培养的NG细胞系,当病毒滴度为2×108时,转集率达到50%,当滴度为2×109时,转染率达100%,有较好的量效关系;固定病毒液度为1010,培养2~16h,细胞的转染率随时间延长而提高,有较好的时效关系。将Ad5CMVlacZ注射到大鼠黑质部位后,分别于注射后3~120d取脑、切片、X-gal染色,发现黑质局部从第7d开始有部分蓝染,第10d达高峰,注射局部感染率100%;90d时开始下降,持续至120d;纹状体等其它部位无蓝染.上述结果提示,腺病毒载体介导的标记基因可在培养的神经细胞系和中脑黑质部位高效表达,为进一步开展中枢神经系统退变性疾病尤其是帕金森氏病的基因治疗奠定基础。  相似文献   
106.
本文测定了30例正常育龄妇女,28例正常妊娠以及36例妊娠征患者血浆中降钙素基因相关肽的水平,结果发现,轻度妊高生与正常妊娠相比,血浆中CGRPK是有下降但不显著(P〉0.05)。中重度妊高征与正常妊娠相比,差异有非常显著性,因此推测,妊娠状态下血浆中降钙素基因相关肽的减少可能是妊娠高血压症产生的重要原因之一。  相似文献   
107.
Epididymis is a site of sperm maturation and storage. Limited and directed-proteolysis regulated by plasminogen activator (PA), plasminogen activator inhibitor type-1 (PAI-1) and other related factors may play an essential role in these processes. Our previous studies have demonstrated that rat epididymis expressed luteinizing hormone receptor (LHR), tissue type (t) and urokinase type (u)PA, mRNAs, and tPA activity was stimulated in vitro by human chorionic gonoadotrophin (HCG). In the present study we further examined localization of mRNAs for tPA, uPA, LHR, androgen receptor (AR), as well as inhibin subunits alpha, betaA and betaB in rhesus monkey epididymis. Using in-situ hybridization with digoxygenin-labelled cRNA probes, we have demonstrated that tPA and PAI-1 mRNAs were localized in epithelial cells of adult monkey epididymis. uPA mRNA was localized in the same areas, but to a much smaller extent. tPA, uPA and PAI-1 mRNAs were greatly expressed in the caput and corpus of adult epididymis than in other regions. In-vitro experiments showed that both tPA and uPA activities in epididymal cells were dramatically stimulated by HCG, but not by follicle stimulating hormone (FSH). LHR (but not FSH receptor) and AR mRNAs were localized in the epithelial cells of the epididymis. However, LHR mRNA was detected in both adult and immature infant monkeys, whereas AR was found only in the adult. Inhibin alpha, betaA and betaB mRNAs were also detected in this organ, betaA mRNA being more strongly expressed in the caput than in other regions of the epididymis. We suggest that LH and androgen may be the key hormones in coordination with the PA-PAI-1 system in regulating epididymal differentiation and sperm maturation.   相似文献   
108.
可切削渗透陶瓷玻璃料对不同堆积密度氧化铝基体的渗透   总被引:1,自引:0,他引:1  
探讨了可切削渗透陶瓷 (Infiltration of Machinable- infiltrated- ceramic,MIC)渗透玻璃在不同堆积密度氧化铝基体中的渗透情况及渗透后复合体的颜色表达。在 110 0℃保温 2 h,将玻璃渗透到不同堆积密度的氧化铝基体中 ,分别测得其渗透深度和颜色参数。扫描电镜观察玻璃—氧化铝复合体断面。玻璃渗透深度的平方与氧化铝堆积密度有直线负相关关系 ,最小渗透深度为 3.0 92 m m。复合体的颜色系数与氧化铝堆积密度无相关性。渗透复合体在断裂过程中可见 :裂纹偏转、晶体拔出和穿晶断裂。本实验证实 MIC渗透玻璃的渗透性能达到临床要求。渗透后的复合体颜色稳定 ,强度可靠  相似文献   
109.
ESWL实际焦点位置的理论和数值分析   总被引:6,自引:1,他引:6  
从理论和数值模拟两个方面分析了ESWL中的非线性的动力学焦点和实际焦点的位置 ,给出三种典型的动力学焦点的数值结果 ,说明非线性动力学焦点和实际焦点一般不是几何焦点 ,指出正确确定实际焦点在ESWL技术中的重要性。  相似文献   
110.
The etiology of systemic lupus erythematosus (SLE) is very complex, and genetic factors appear to play a significant role in susceptibility to SLE, in determining the disease expression, and in the autoantibody profiles of individuals with SLE. DNA methyltransferase-1 (DNMT1) is a major enzyme that determines genomic methylation patterns and both maintains methyltransferase and exhibits de novo DNA methylation activity in vivo. In order to clarify the association of DNMT1 polymorphisms with SLE, we scrutinized the genetic polymorphisms in exons and their boundaries of DNMT1, including the –1,500 bp promoter region, by direct sequencing in 24 Korean individuals. Twenty-nine sequence variants were identified: two in 5UTR, six in exons, and 21 in introns. Eight of these polymorphisms were selected for a larger-scale genotyping (n=680) by considering their allele frequencies, haplotype-tagging status, and linkage disequilibrium coefficiencies (LDs) among polymorphisms. The associations between DNMT1 polymorphisms and the clinical profiles of SLE were analyzed. No significant associations with the risk of SLE were detected. However, further analyses of association with autoantibody production among SLE patients revealed that one nonsynonymous SNP, +14463G>C (V120L) in exon 4, was weakly associated with an increased risk of anti-La antibody production (P=0.04), although the significance could not be retained after correction of multiple tests. The DNMT1 variations and haplotypes clarified in this study would provide valuable information for future genetic studies of other autoimmune diseases.  相似文献   
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