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排序方式: 共有645条查询结果,搜索用时 328 毫秒
61.
Yoneyama Y Sawa R Suzuki S Doi D Yoneyama K Otsubo Y Araki T 《Clinica chimica acta; international journal of clinical chemistry》2002,322(1-2):169-173
BACKGROUND: Preeclampsia is characterized by endothelial cell dysfunction, and lipid peroxidation and alterations of immune responses may be involved in the pathogenesis of this disease. The aim of the present study was to examine changes in plasma malondialdehyde (MDA) level, an indicator of lipid peroxidation, and adenosine deaminase (ADA) activity, as a marker of T-cell activation in preeclampsia, and evaluated the possible relationship between those parameters in the pathogenesis of preeclampsia. METHODS: Plasma levels of MDA, an end product of lipid peroxidation induced by reactive oxygen species, and ADA activities were measured in 26 women with preeclampsia and 26 normal pregnancies. RESULTS: In preeclampsia, plasma MDA level and ADA activity averaged 0.43 +/- 0.03 micromol/l and 15.8 +/- 0.8 U/l, respectively, which were significantly higher than those of normal pregnancy (0.31 +/- 0.02 micromol/l and 9.5 +/ -0.6 U/l, respectively) (P<0.05). In addition, plasma MDA level was positively correlated to ADA activity in preeclampsia (r=0.52, P<0.05). CONCLUSIONS: These results suggest that enhanced lipid peroxidation and T-cell activation, and the presence of possible interrelationship and cross talk between those parameters may be related at least partly to the pathogenesis of preeclampsia. 相似文献
62.
Ochi A Otsubo H Donner EJ Elliott I Iwata R Funaki T Akizuki Y Akiyama T Imai K Rutka JT Snead OC 《Epilepsia》2007,48(2):286-296
PURPOSE: To characterize the spatial and temporal course of ictal high-frequency oscillations (HFOs) recorded by subdural EEG in children with intractable neocortical epilepsy. METHODS: We retrospectively studied nine children (four girls, five boys; 4-17 yr) who presented with intractable extrahippocampal localization-related epilepsy and who underwent extraoperative video subdural EEG (1000 Hz sampling rate) and cortical resection. We performed multiple band frequency analysis (MBFA) to evaluate the frequency, time course, and distribution of ictal HFOs. We compared ictal HFO changes before and after clinical onset and postsurgical seizure outcomes. RESULTS: Seventy-eight of 79 seizures showed HFOs. We observed wide-band HFOs ( approximately 250 Hz, approximately 120 electrodes) in six patients either with partial seizures alone (three patients) or with epileptic spasms (three patients). Three patients with partial seizures that secondarily generalized had wide-band HFOs ( approximately 170 Hz) before clinical onset and sustained narrow-band HFOs (60-164 Hz) with electrodecremental events after clinical onset ( approximately 28 electrodes). In four postoperatively seizure-free patients, more electrodes recorded higher-frequency HFOs inside the resection area than outside before and after clinical seizure onset. In five patients with residual seizures, electrodes recorded more HFOs that were of higher or equal frequency outside the surgical area than inside after clinical onset. CONCLUSION: For partial seizures alone and epileptic spasms, more electrodes recorded only wide-band HFOs; for partial seizures that secondarily generalized, fewer electrodes recorded wide-band HFOs, but in these seizures electrodes also recorded subsequent sustained narrow-band ictal HFOs. Resection of those brain regions having electrodes with ictal, higher HFOs resulted in postsurgical seizure-free outcomes. 相似文献
63.
Surgical treatment for acute symptomatic refractory status epilepticus: a case report 总被引:1,自引:0,他引:1
Mohamed IS Otsubo H Imai K Shroff M Sharma R Chuang SH Donner E Drake J Snead OC 《Journal of child neurology》2007,22(4):435-439
A previously healthy 10-year-old boy developed generalized convulsive status epilepticus following a mild febrile illness. Prolonged video-electroencephalographic monitoring revealed frequent right hemispheric electrographic seizures that were refractory to high-dose suppressive therapy. Ictal and interictal magnetoencephalography demonstrated dipole sources projecting from the right mesial temporal region. Diffusion-weighted imaging showed restricted diffusion involving the right hippocampus. Right anterior temporal lobectomy resulted in cessation of status epilepticus. At 1-year follow-up, he attends regular school and has infrequent nocturnal seizures on chronic antiepileptic drug therapy. Surgical treatment should be considered to stop status epilepticus in selected cases of acute symptomatic refractory status epilepticus with no preexisting epilepsy or magnetic resonance imaging abnormalities and may avoid the complications associated with prolonged high-dose suppressive therapy. 相似文献
64.
Urano J Sato T Matsuo T Otsubo Y Yamamoto M Tamanoi F 《Proceedings of the National Academy of Sciences of the United States of America》2007,104(9):3514-3519
Rheb is a unique member of the Ras superfamily GTP-binding proteins. We as well as others previously have shown that Rheb is a critical component of the TSC/TOR signaling pathway. In fission yeast, Rheb is encoded by the rhb1 gene. Rhb1p is essential for growth and directly interacts with Tor2p. In this article, we report identification of 22 single amino acid changes in the Tor2 protein that enable growth in the absence of Rhb1p. These mutants also exhibit decreased mating efficiency. Interestingly, the mutations are located in the C-terminal half of the Tor2 protein, clustering mainly within the FAT and kinase domains. We noted some differences in the effect of a mutation in the FAT domain (L1310P) and in the kinase domain (E2221K) on growth and mating. Although the Tor2p mutations bypass Rhb1p's requirement for growth, they are incapable of suppressing Rhb1p's requirement for resistance to stress and toxic amino acids, pointing to multiple functions of Rhb1p. In mammalian systems, we find that mammalian target of rapamycin (mTOR) carrying analogous mutations (L1460P or E2419K), although sensitive to rapamycin, exhibits constitutive activation even when the cells are starved for nutrients. These mutations do not show significant difference in their ability to form complexes with Raptor, Rictor, or mLST8. Furthermore, we present evidence that mutant mTOR can complex with wild-type mTOR and that this heterodimer is active in nutrient-starved cells. 相似文献
65.
66.
Amna Al-Futaisi Brenda Banwell Ayako Ochi Justine Hew Bill Chu Makoto Oishi Hiroshi Otsubo 《Clinical neurophysiology》2005,116(5):1113-1117
OBJECTIVE: We report on a 27-month-old female with atypical early infantile epileptic encephalopathy (EIEE), who developed tonic spasms, partial seizures and myoclonic jerks along with episodic bradycardia at 5 days. METHODS: We recorded digital electroencephalography (EEG) using either an 11-channel neonatal montage or 19 channel scalp electrodes, at 200 Hz sampling rate, and a single reference for a minimum of 30 min. RESULTS: At 18 days EEG showed suppression-burst (SB) patterns during wakefulness and sleep. Tonic spasms concomitant with bursts recorded as brief, low-amplitude fast waves. EEG at 8 months showed increased amplitude of bursts to 1 mV and extension of suppression periods to 65 s. By increasing recording sensitivity, we detected focal epileptiform discharges of slow rhythmic sharp and slow waves building to 30 microV during suppression periods. Status epilepticus occurred at 16 months. EEG at 27 months returned to the previous SB pattern with rare partial seizures. CONCLUSIONS: This report is the first to demonstrate clinically silent focal EEG seizures during prolonged suppression periods in atypical EIEE by off-line digital EEG. SIGNIFICANCE: Digital EEG sensitivity can reveal covert electrical activity during suppression periods in epileptic neonates and infants. 相似文献
67.
The diagnostic and prognostic significance of the absence of simple partial seizures (SPS) immediately preceding complex partial seizures (CPS) was examined in patients with temporal lobe epilepsy. The status of self-reported SPS in 193 patients with temporal lobe epilepsy who had surgical therapy more than 2 years ago was reviewed. Before surgery, 37 patients never experienced SPS before CPS (Group A), 156 patients either always or occasionally had SPS before CPS (Group B). The frequency of mesial temporal sclerosis (MTS) was lower and the age at onset of epilepsy was higher in Group A. The seizure focus was in the language-dominant temporal lobe in 73% of the cases in Group A, compared with 40% in Group B. The surgical outcome did not differ between the two groups. The findings suggest that temporal lobe seizures without preceding SPS tend to originate in the language-dominant temporal lobe that contains a pathologic etiology other than MTS, especially in the lateral temporal lobe. The surgical outcome in patients without SPS is similar to that in patients with SPS. 相似文献
68.
Longitudinal neuroendocrine changes assessed by dexamethasone/CRH and growth hormone releasing hormone tests in psychotic depression 总被引:1,自引:0,他引:1
Owashi T Otsubo T Oshima A Nakagome K Higuchi T Kamijima K 《Psychoneuroendocrinology》2008,33(2):152-161
Although psychotic depression has been reported to exhibit a greater degree of dysregulation of hypothalamic-pituitary-adrenocortical (HPA) function than non-psychotic depression, little is known concerning hypothalamic-pituitary-somatotropic (HPS) function in psychotic depression and how neuroendocrine function changes after treatment. To investigate the longitudinal changes in HPA and HPS system function in psychotic depression, we performed repeated dexamethasone/corticotropin releasing hormone (DEX/CRH) tests and growth hormone (GH) releasing hormone (GHRH) tests in inpatients with major depressive disorder. The psychotic depression group exhibited greater elevation of ACTH responses to the DEX/CRH test and stronger decreases in GH responses to the GHRH test than the non-psychotic depression group at admission. At discharge, the neuroendocrine responses to the DEX/CRH test of the psychotic depression group were still stronger than those of the non-psychotic depression group, though there were no significant differences in severity of depression between the groups. There were significant longitudinal changes in neuroendocrine responses to the DEX/CRH test between admission and discharge. The psychotic depression group exhibited increased GH responses to GHRH at discharge compared with those at admission, whereas no significant longitudinal change in GH response was found in the non-psychotic depression group. Consequently, there were no significant differences in GH responses to GHRH between the psychotic and non-psychotic depression groups at discharge. The results of GHRH test showed no significant relationships with severity of depression except psychotic features and the results of the DEX/CRH test. Our findings suggest that the HPS axis may be associated with psychotic features rather than general severity of depression. Further longitudinal studies are needed to clarify the role of HPS function in psychotic depression and whether sustained dysregulation of HPA function in psychotic depression is associated with a poor outcome after discharge. 相似文献
69.
70.
Lan S Chen Hiroshi Otsubo Ayako Ochi W W Lai D Sutoyo O Carter Snead 《Journal of clinical neurophysiology》2002,19(3):192-203
The objective of this study was to determine whether the animation of electrical activity recorded on ictal electrocorticograms (ECoGs) can demonstrate the propagation of seizure discharges from the epileptogenic zone (EZ) to the surrounding cortical area. A computer program, continuous potential display (CPD), was designed to animate the color-coded potential changes in 5-msec intervals at each recorded site. This program was used to analyze 35 ictal ECoGs recorded by subdural grid electrodes from 11 subjects who underwent epilepsy surgery for intractable partial seizures. Continuous potential display demonstrated recurrent cycles of seizure propagation from the EZ to the surrounding cortical area even when seizure discharges appeared widespread on ECoG. Hence, the EZ could be mapped at any time during the seizure course. The EZ mapped by analyzing a small fraction of ECoG during widespread seizure discharges using CPD only overlapped 69 +/- 24% (mean +/- standard deviation) of the surgical area. The EZ mapped by CPD had 34 +/- 22% false positives and 35 +/- 27% false negatives. Animation of potential changes recorded by ictal ECoG can assist in studying the temporal and spatial patterns of seizure propagation and in mapping the EZ for surgical resection. 相似文献