全文获取类型
收费全文 | 512篇 |
免费 | 36篇 |
国内免费 | 1篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 6篇 |
妇产科学 | 3篇 |
基础医学 | 84篇 |
口腔科学 | 9篇 |
临床医学 | 20篇 |
内科学 | 178篇 |
皮肤病学 | 46篇 |
神经病学 | 6篇 |
特种医学 | 63篇 |
外科学 | 35篇 |
综合类 | 6篇 |
预防医学 | 11篇 |
眼科学 | 10篇 |
药学 | 9篇 |
肿瘤学 | 62篇 |
出版年
2023年 | 5篇 |
2022年 | 3篇 |
2021年 | 16篇 |
2020年 | 5篇 |
2019年 | 7篇 |
2018年 | 22篇 |
2017年 | 7篇 |
2016年 | 21篇 |
2015年 | 17篇 |
2014年 | 23篇 |
2013年 | 22篇 |
2012年 | 35篇 |
2011年 | 39篇 |
2010年 | 24篇 |
2009年 | 21篇 |
2008年 | 32篇 |
2007年 | 36篇 |
2006年 | 28篇 |
2005年 | 25篇 |
2004年 | 24篇 |
2003年 | 22篇 |
2002年 | 17篇 |
2001年 | 10篇 |
2000年 | 1篇 |
1999年 | 4篇 |
1998年 | 4篇 |
1997年 | 8篇 |
1996年 | 5篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1991年 | 4篇 |
1990年 | 7篇 |
1989年 | 7篇 |
1988年 | 9篇 |
1987年 | 6篇 |
1986年 | 7篇 |
1985年 | 9篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1973年 | 1篇 |
1929年 | 1篇 |
1909年 | 1篇 |
排序方式: 共有549条查询结果,搜索用时 15 毫秒
71.
Tomomitsu Higashi Masato Kobayashi Hiromi Wakao Yoshinori Jinbu 《European journal of nuclear medicine and molecular imaging》1989,15(3):152-156
Previously, we reported that the deposition of 67Ga into malignant tumors may be a sensitive index of proliferative activity in tumor cells. For the purpose of elucidation of this hypothesis, we investigated the relationship between the accumulation of 67Ga into malignant tumor cells and the intra cellular ATP metabolism in vitro.The uptake of 67Ga into tumor cells was inhibited by adding NaF which is an inhibitor of ATP production. Furthermore, the uptake of 67Ga into tumor cells was strongly inhibited by adding ouabain which is a specific inhibitor of Na+–K+-ATPase. From these in vitro results, it was concluded that there is a correlation between 67Ga uptake and intra cellular ATP metabolism in tumor cells. 相似文献
72.
Tomomitsu Higashi Hiromi Wakao Masuji Yamaguchi Koreaki Suga 《European journal of nuclear medicine and molecular imaging》1988,14(3):155-158
Previously we reported that the deposition of 67Ga into malignant tumor may be a sensitive index of proliferative activity in tumor cells. For the purpose of elucidation of this hypothesis, we investigated the relationship between the accumulation of 67Ga into malignant tumor cells and the cell cycle in vitro. We discovered that the uptake of both 67Ga and 59Fe into synchronized mouse tumor cells reaches a peak at the G2 stage which precedes cellular proliferation. Both iron and transferrin are specifically required by cells in culture for cell division, and the fact that 67Ga and 59Fe uptake into tumor cells peaks at the G2 stage of the cell cycle suggests that there is a correlation between 67Ga uptake and the rate of cellular proliferation in malignant tumor cells. 相似文献
73.
The importance of physical activity in the development and maintenance of bone mineral density (BMD) is widely accepted. However, the effects on cortical BMD have not been clarified in detail. The present study examined bilateral asymmetries in cortical BMD of the tibia using peripheral quantitative computed tomography. Subjects comprised 37 young male athletes and 57 controls (age range, 18-28 years). BMD and geometrical indices were determined in bilateral tibiae. Cortical and trabecular BMD were calculated at the diaphysis and distal metaphysis, respectively. Cortical width, periosteal cross-sectional area, and cross-sectional moment of inertia were calculated using tomographic data of the tibial diaphysis. In athletes, the non-dominant leg showed greater cortical BMD than the dominant leg (mean difference, 5.42%; P < 0.0001). Cortical width and moment of inertia were also greater in the non-dominant leg. Periosteal area displayed no significant difference between legs. The control group exhibited similar results except for cortical BMD. No differences in trabecular BMD were noted between legs in either athletes or controls. These results implies the existence of mechanisms for the mechanical adaptation of cortical BMD. Dominant leg is used for mobility or manipulation whereas the non-dominant leg contributes to support the actions of the dominant leg. Loading differences in bilateral legs in young athletes might affect the remodeling rate leading to the side-to-side differences in cortical BMD. 相似文献
74.
Morimoto T Miyoshi T Matsuda S Tano Y Fujikado T Fukuda Y 《Investigative ophthalmology & visual science》2005,46(6):2147-2155
PURPOSE: To investigate the effect of transcorneal electrical stimulation (TES) on the survival of axotomized RGCs and the mechanism underlying the TES-induced neuroprotection in vivo. METHODS: Adult male Wistar rats received TES after optic nerve (ON) transection. Seven days after the ON transection, the density of the surviving RGCs was determined, to evaluate the neuroprotective effect of TES. The levels of the mRNA and protein of insulin-like growth factor (IGF)-1 in the retina after TES were determined by RT-PCR and Northern and Western blot analyses. The localization of IGF-1 protein in the retina was examined by immunohistochemistry. RESULTS: TES after ON transection increased the survival of axotomized RGCs in vivo, and the degree of rescue depended on the strength of the electric charge. RT-PCR and Northern and Western blot analyses revealed a gradual upregulation of intrinsic IGF-1 in the retina after TES. Immunohistochemical analysis showed that IGF-1 immunoreactivity was localized initially in the endfeet of Muller cells and then diffused into the inner retina. CONCLUSIONS: TES can rescue the axotomized RGCs by increasing the level of IGF-1 production by Muller cells. These findings provide a new therapeutic approach to prevent or delay the degeneration of retinal neurons without the administration of exogenous neurotrophic factors. 相似文献
75.
Fukuda R Kawada H Ogawa Y Yanagi H Kojima S Urano T Chie I Onizuka M Fukagawa S Shimada M Suzuki R Takagi A Hotta T 《The Tokai journal of experimental and clinical medicine》2005,30(2):133-136
Lung involvement is rare in Waldenstr?m macroglobulinemia (WM). We encountered a male patient with WM who complained of breathlessness. Chest X-ray revealed diffuse infiltrative shadow throughout the both lungs. Transbronchial biopsy showed infiltration of atypical plasmacytoid lymphocytes and non-caseating granuloma. We treated the patients with fludarabine phosphate, and both his symptom and X-ray findings were then improved. To our knowledge, this is the first case showing non-caseating granuloma with lung involvement of WM. We discuss a mechanism of non-caseating granuloma formation in this case. 相似文献
76.
Hamady Dieng Rahman GM Saifur Abu Hassan Ahmad MR Che Salmah Al Thbiani Aziz Tomomitsu Satho Fumio Miake Zairi Jaal Sazaly Abubakar Ronald Enrique Morales 《Asian Pacific Journal of Tropical Biomedicine》2012,2(3):228-232
Objective
To identify the unusual breeding sites of two dengue vectors, i.e. Aedes albopictus (Ae. albopictus) and Aedes aegypti (Ae. aegypti).Methods
During the second half of 2010, we performed an occasional survey in rural (Teluk Tempoyak) and urban (Gelugor) areas of Penang Island, Malaysia, to identify cryptic breeding sites.Results
In the rural area, we found heterogeneous immature stages of Ae. albopictus in the water bowl of an encaged bird. We also observed Ae. aegypti eggs deposited in the flush tank of a toilet in the urban area.Conclusions
It can be concluded that both breeding patterns can increase contact with hosts (humans and birds) and presumably population densities of Ae. albopictus and Ae. aegypti, thereby potentially boosting the risks for spread and transmission of arboviral diseases. 相似文献77.
Promising efficacy of the sirolimus-eluting stent in patients with acute myocardial infarction 总被引:1,自引:0,他引:1
Gochi T Umeda H Yoshida N Maekawa H Watanabe K Okada T Katoh T Asai T Tani T Yokoya M Murakami Y Matsushita T Shimizu T Ozaki Y Hiramitsu S Murohara T Nomura M Morimoto S Hishida H 《Journal of cardiology》2006,48(5):253-261
OBJECTIVES: This study investigated the safety and efficacy of sirolimus-eluting stents (SESs) on early and late outcomes in patients with acute myocardial infarction. METHODS: A series of 100 consecutive patients (September 2004 to November 2005)with acute myocardial infarction undergoing primary stenting using SES ptember 24 hr) was compared with 100 consecutive patients (September 2003 to August 2004) treated with bare metal stent (BMS). The frequency of major adverse cardiac events (MACE) and stent thrombosis, and status of ticlopidine administration were assessed at 270 days. RESULTS: The rates of premature discontinuation of ticlopidine (SES group <3 months: 11%, BMS group <1 month: 11%, p = NS) and stent thrombosis (SES group: 1%, BMS group: 0%, p = NS) were similar in the two groups. At follow-up, restenosis rate and target vessel revascularization rate were lower in the SES group(4% vs 19%, p < 0.001 and 4% vs 10%, p = 0.149, respectively). Furthermore, the occurrence of MACE at 270 days was significantly less frequent in the SES group compared with the BMS group (6% vs. 17%, p = 0.038). Multivariate analysis showed SES use tended to predict 270-day MACE (hazard ratio 0.37, 95% confidence interval 0.14-1.02, p = 0.055). Culprit lesion located in the left main trunk was identified as an independent predictor of 270-day MACE (hazard ratio 5.43, 95% confidence interval 1.07-27.59, p = 0.041). CONCLUSIONS: The use of a SES was not associated with increased risk of stent thrombosis compared with a BMS. With lower rates of restenosis and subsequent target vessel revascularization, SES placement could provide superior outcomes in patients with acute myocardial infarction. 相似文献
78.
Fukahori S Tsuru T Tanikawa K Akiyoshi K Asagiri K Tanaka Y Kage M Kojiro M Mizote H Yagi M 《Surgery today》2007,37(9):811-816
Mesenchymal hamartoma of the liver (MHL) is an uncommon benign tumor found primarily in children younger than 2 years of age.
We report a rare case of MHL with a daughter nodule and atypical histological findings in a 14-month-old girl. On admission,
computed tomography, magnetic resonance imaging, and angiography showed a solid hypovascular mass with a central cystic area
in the liver. Laparotomy revealed a tumor, 8 cm in size, occupying segment 5 and parts of segments 4 and 6 of the liver, and
a small nodule, 10 mm in size, in segment 7. Thus, we performed a partial hepatic resection (S4–6) and tumor extirpation (S7).
The histological findings of both tumors were the same, but atypical of MHL. Recent studies on the pathogenesis of this tumor
have found neoplastic features such as genetic anomalies and malignant transformation. These findings suggest that the conventional
approach of completely resecting the tumor whenever possible is the best treatment. 相似文献
79.
Shibata T Arisawa T Tahara T Yoshioka D Maruyama N Fujita H Kamiya Y Nakamura M Nagasaka M Iwata M Takahama K Watanabe M Hirata I Nakano H 《Digestive diseases and sciences》2009,54(1):70-74
Background Heat shock protein 70-2 (HSP70-2) has a cytoprotective role in various conditions and also protects the gastric mucosa. Recently,
polymorphism of HSP70-2 at position 1267 was suggested to be associated with carcinogenesis. We investigated the association
of this polymorphism with the risk of gastric cancer in the present study. Methods We examined 223 patients (159 men and 64 women, mean age 64.8 years) with gastric cancer who underwent gastrointestinal endoscopy
at our department. The controls were 200 age-matched patients (140 men and 60 women) without gastric cancer diagnosed by gastrointestinal
endoscopy. Genotyping was done by PCR-based restriction fragment length polymorphism analysis, and the PCR products were digested
with PstI. The two allelic forms, corresponding to the presence or absence of the PstI site, were designated as the P1 allele and P2 allele, respectively. Logistic regression analysis was performed to calculate
an odds ratios (ORs) for differences of HSP70-2 polymorphism between the two groups. Results Among the 223 patients with gastric cancer, 46 (20.6%) had P1/P1, 177 (79.4%) were P1 carriers, and 6 (2.7%) were P2/P2.
In the control group, 33 (16.5%) patients had P1/P1 polymorphism, 167 (83.5%) were P1 carriers, and 12 (6.0%) were P2/P2.
The OR for gastric cancer of subjects with P2/P2 polymorphism relative to P1 carriers was 0.43 (95% CI = 0.16–1.17) (P = 0.097). Among females, the OR for gastric cancer of subjects with P2/P2 polymorphism relative to P1 carriers was 0.10 (95%
CI = 0.012–0.838) (P = 0.014). This polymorphism was also associated with a lower risk of middle third cancer (OR = 0.13; 95% CI = 0.02–1.00).
Conclusions P2/P2 polymorphism of HSP70-2 at position 1267 was associated with a lower risk of gastric cancer in females. 相似文献
80.
Ando K Yahata T Sato T Miyatake H Matsuzawa H Oki M Miyoshi H Tsuji T Kato S Hotta T 《Blood》2006,107(8):3371-3377
To characterize human hematopoietic stem cells (HSCs), xenotransplantation techniques such as the severe combined immunodeficiency (SCID) mouse repopulating cell (SRC) assay have proven the most reliable methods thus far. While SRC quantification by limiting dilution analysis (LDA) is the gold standard for measuring in vitro expansion of human HSCs, LDA is a statistical method and does not directly establish that a single HSC has self-renewed in vitro. This would require a direct clonal method and has not been done. By using lentiviral gene marking and direct intra-bone marrow injection of cultured CD34+ CB cells, we demonstrate here the first direct evidence for self-renewal of individual SRC clones in vitro. Of 74 clones analyzed, 20 clones (27%) divided and repopulated in more than 2 mice after serum-free and stroma-dependent culture. Some of the clones were secondary transplantable. This indicates symmetric self-renewal divisions in vitro. On the other hand, 54 clones (73%) present in only 1 mouse may result from asymmetric divisions in vitro. Our data demonstrate that current ex vivo expansion conditions result in reliable stem cell expansion and the clonal tracking we have employed is the only reliable method that can be used in the development of clinically appropriate expansion methods. 相似文献