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971.
Fukuda N Nakayama M Jian T Satoh C Nakayama T Soma M Izumi Y Kanmatsuse K 《American journal of hypertension》2003,16(2):129-134
Insulin resistance is involved in the pathogenesis of type 2 diabetes, hypertension, and atherosclerosis. Angiotensin (Ang) converting enzyme inhibitors and Ang II type 1 receptor antagonists improve insulin resistance in patients with essential hypertension, which suggest that tissue Ang II is involved in insulin resistance in patients with hypertension. To investigate the participation of tissue Ang II in insulin resistance associated with hypertension, we evaluated the Ang II-generating system in leukocytes and its relation to insulin resistance in patients with essential hypertension. Eighteen patients with essential hypertension participated in this study. Ang II was separated from leukocytes by reversed-phase high-performance liquid chromatography and measured by radioimmunoassay. Insulin resistance was evaluated by determining the steady-state of plasma glucose (SSPG) concentration. The Ang I- and Ang II-generating activities were evaluated in human leukocytes. Human leukocytes have Ang I- and Ang II-generating activities. The Ang II-generating activity was significantly inhibited by pepstatin A. Leukocyte Ang II level does not correlate with BP or plasma Ang II level in patients with essential hypertension. Leukocyte Ang II level strongly correlates with SSPG concentration, and significantly correlates with body mass index and plasma insulin, and with leptin levels in patients with essential hypertension. Leukocyte Ang II may be directly associated with insulin resistance. 相似文献
972.
Prognostic factors for chronic active Epstein-Barr virus infection 总被引:11,自引:0,他引:11
Kimura H Morishima T Kanegane H Ohga S Hoshino Y Maeda A Imai S Okano M Morio T Yokota S Tsuchiya S Yachie A Imashuku S Kawa K Wakiguchi H;Japanese Association for Research on Epstein-Barr Virus Related Diseases 《The Journal of infectious diseases》2003,187(4):527-533
Chronic active Epstein-Barr virus infection (CAEBV) is a high-mortality and high-morbidity disease. To clarify the prognostic factors, a national survey was performed in Japan, and data for 82 patients who met the criteria for CAEBV were analyzed. Of these 82 patients, 47 were alive and 35 had already died. Multivariate analysis revealed that thromobocytopenia and age at disease onset were correlated with mortality. The probability of 5-year survival was 0.45 for older patients (onset age, > or = 8 years), 0.94 for younger patients (P<.001), 0.38 for patients with thrombocytopenia (platelet count < 12 x 10(4) platelets/microL at diagnosis), and 0.76 for patients without thrombocytopenia (P=.01). Furthermore, patients with T cell infection by EBV had shorter survival times than patients with natural killer cell infection (probability of 5-year survival, 0.59 vs. 0.87; P<.009). Patients with CAEBV with late onset of disease, thrombocytopenia, and T cell infection had significantly poorer outcomes. 相似文献
973.
974.
Tskitishvili E Komoto Y Kinugasa Y Kanagawa T Song M Mimura K Tomimatsu T Kimura T Shimoya K 《Acta obstetricia et gynecologica Scandinavica》2007,86(8):915-920
BACKGROUND: S100B protein is a unique calcium-binding protein. Its biological role within the cell populations is not completely defined. Some pathological conditions that develop during pregnancy could affect S100B concentrations in the amniotic fluid, cord blood, and maternal serum. The aim of our study was to assess the correlation between S100B protein expression in the amnion, amniotic fluid and gestational age in the third trimester of uncomplicated pregnancies. METHODS: Amnion, amniotic fluid, maternal peripheral and umbilical cord blood samples were collected from healthy women who delivered at 31-36 weeks (n=17), 37-40 weeks (n=22), and 41-42 weeks (n=21). The expression of S100B in the amnion was assessed by immunohistochemistry and real-time (RT)-PCR, and its concentrations in amniotic fluid, maternal and cord blood sera were determined by ELISA. RESULTS: The S100B protein expression in the amnion and its concentrations in amniotic fluid, maternal and cord blood sera of patients in the third trimester were not significantly different at various gestational ages. CONCLUSIONS: The S100B protein expression in the amnion and the S100B protein concentrations in amniotic fluid, maternal and cord blood do not vary significantly in the third trimester of uncomplicated pregnancies. 相似文献
975.
976.
Taku Wakabayashi Hisamichi Naito Tomohiro Iba Kohji Nishida Nobuyuki Takakura 《Investigative ophthalmology & visual science》2022,63(4)
PurposeCD157 (also known as Bst1) positive vascular endothelial stem cells (VESCs), which contribute to vascular regeneration, have been recently identified in mouse organs, including the retinas, brain, liver, lungs, heart, and skin. However, VESCs have not been identified in the choroid. The purpose of this study was to identify VESCs in choroidal vessels and to establish the protocol to isolate retinal and choroidal VESCs.MethodsWe established an efficient protocol to create single-cell suspensions from freshly isolated mouse retina and choroid by enzymatic digestion using dispase, collagenase, and type II collagenase. CD157-positive VESCs, defined as CD31+CD45−CD157+ cells, were sorted using fluorescence-activated cell sorting (FACS).ResultsIn mouse retina, among CD31+CD45− endothelial cells (ECs), 1.6 ± 0.2% were CD157-positive VESCs, based on FACS analysis. In mouse choroid, among CD31+CD45− ECs, 4.5 ± 0.4% were VESCs. The CD157-positive VESCs generated a higher number of EC networks compared with CD157-negative non-VESCs under vascular endothelial growth factor (VEGF) in vitro cultures. The EC network area, defined as the ratio of the CD31-positive area to the total area in each field, was 4.21 ± 0.39% (retinal VESCs) and 0.27 ± 0.12% (retinal non-VESCs), respectively (P < 0.01). The EC network area was 8.59 ± 0.78% (choroidal VESCs) and 0.14 ± 0.04% (choroidal non-VESCs), respectively (P < 0.01). The VESCs were located in large blood vessels but not in the capillaries.ConclusionsWe confirmed distinct populations of CD157-positive VESCs in both mouse retina and choroid. VESCs are located in large vessels and have the proliferative potential. The current results may open new avenues for the research and treatment of ocular vascular diseases. 相似文献
977.
Aortic stenosis(AS)is the most common valvular disease in the older adults'population,and its prevalence will likely continue to increase with the aging society.[1]Surgical aortic valve replacement(SAVR)for elderly patients is known as a high-risk procedure.[2-4]Transcatheter aortic valve implantation(TAVI)is a reasonable alternative to SAVR for older and inoperable patients with AS,and TAVI significantly improves outcomes in elderly patients with severe AS and serious comorbidities,[5,6]TAVI achieves a 20%relative reduction in mortality compared with SAVR. 相似文献
978.
Lingling Kong Namiko Hoshi Yunlong Sui Yasutaka Yamada Ryutaro Yoshida Makoto Ooi Zibin Tian Ikuo Kimura Yuzo Kodama 《Medical principles and practice》2022,31(1):39
ObjectiveG protein-coupled receptor 43 (GPR43), a receptor for short-chain fatty acids, plays a role in suppressing tumor growth; however, the detailed underlying mechanism needs to be comprehensively elucidated. In this study, we investigated the role of GPR43 in inhibiting tumor growth using Apc<sup>Min/+</sup>, a murine model of intestinal tumors.Materials and MethodsUsing GPR43<sup>−/−</sup> Apc<sup>Min/+</sup> and GPR43<sup>+/−</sup> Apc<sup>Min/+</sup> mice, the number of tumors was analyzed at the end of the experimental period. Immunohistochemistry, quantitative polymerase chain reaction, and Western blotting were performed to analyze cellular proliferation and proliferation-associated signal pathways.ResultsOur results revealed that GPR43 deficiency resulted in increased tumor numbers in Apc<sup>Min/+</sup> mice. Ki67 was highly expressed in GPR43<sup>−/−</sup> mice (p > 0.05). Increased expression levels of proinflammatory cytokines, including interleukin-6 and tumor necrosis factor-α, and amino acid transporters were not observed in GPR43-deficient mice compared to GPR43-sufficient mice. Furthermore, GPR43-deficient tumor tissues showed enhanced mammalian target of rapamycin-mediated phosphorylated ribosomal protein S6 kinase beta-1 (p > 0.05) and phosphorylated eukaryotic translation initiation factor 4E-binding protein 1 (p > 0.05), but not Akt (protein kinase B) phosphorylation (p = 0.7088).ConclusionCollectively, GPR43 affords protection against tumor growth at least partly through inhibition of the mammalian target of rapamycin complex 1 pathway. 相似文献
979.
Yukino Katakura Fuminori Tatsumi Takashi Kusano Masashi Shimoda Kenji Kohara Tomohiko Kimura Atsushi Obata Shuhei Nakanishi Tomoatsu Mune Kohei Kaku Hideaki Kaneto 《Internal medicine (Tokyo, Japan)》2022,61(6):861
A 58-year-old Japanese man was brought to the emergency room due to disturbance of consciousness. He regained consciousness on the day of admission and started taking hospital meals, but he needed intravenous glucose administration for eight days. The total amount of glucose administration was 4,464 g. It took over three weeks for exogenous insulin to be almost undetectable. While degludec binds to albumin and exerts glucose-lowering effects for a long time, the above-mentioned period of three weeks was consistent with the half-life of albumin. Hypoglycemia induced by massive dose of insulin degludec is persistent and prominent. 相似文献
980.
Disseminated toxoplasmosis after hematopoietic stem cell transplantation showing unusual magnetic resonance images
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Takahiro Tateno Masahiro Onozawa Junichi Hashiguchi Takashi Ishio Sayaka Yuzawa Satomi Matsuoka Mizuha Kosugi‐Kanaya Kohei Okada Souichi Shiratori Hideki Goto Taichi Kimura Junichi Sugita Masao Nakagawa Daigo Hashimoto Kaoru Kahata Katsuya Fujimoto Tomoyuki Endo Takeshi Kondo Shinya Tanaka Satoshi Hashino Takanori Teshima 《Transplant infectious disease》2017,19(4)
We herein report a patient who had disseminated toxoplasmosis after hematopoietic stem cell transplantation showing atypical clinical presentation and neuroimaging. Parkinsonism symptoms such as muscle rigidity, bradykinesia, tremor, and postural instability were initial manifestations. Magnetic resonance imaging showed diffuse symmetrical lesions of bilateral basal ganglia lacking ringed enhancement. Post‐mortem analysis revealed multiple tachyzoites of Toxoplasma gondii in the basal ganglia, mid brain, cerebellum, and cardiac muscle. 相似文献