易感小鼠花生过敏诱导的中毒反应

花生过敏是造成人ＩｇＥ诱导中毒反应的主要原因之一,本试验组制作Ｃ３Ｈ小鼠动物模型,并给其口服花生蛋白（ＰＮ）及霍乱毒素（ＣＴｘ）,然后在腹腔内注射ＰＮ（溶于明矾中的ＰＮ）,使这种鼠即刻发生高度敏感症状（包括致死性的中毒反应）及花生特异性ＩｇＥ水平增高,血浆息斯地明水平、脱颗粒肥大细胞及血管渗漏水平也大幅度升高。对照组小鼠未给药或仅仅接受了ＣＴｘ,则未显示出花生特异性ＩｇＥ水平升高及中毒症状。本文又分析了几种与中毒反应有关的参数。结果证明,小鼠花生过敏中毒反应与人花生过敏类似,同时小鼠花生过敏中毒反应模型,为研究中毒性免疫病理发生机制及研究更有效的治疗方案提供了一个有用的工具。     

Partial characterization of uterine cells responsible for suppression of murine maternal anti-fetal immune responses

Several studies have demonstrated that uterine cells are capable of suppressing in vitro immune responses in a nonspecific manner. Two types of cells have been implicated as responsible for suppression, cells with the features of macrophages and a small lymphocyte-like cell. In the present study the maternal anti-paternal mixed lymphocyte reaction was used to investigate further the characteristics of uterine suppressor cells. Three distinct suppressor cell populations were identified: highly suppressive macrophages, small lymphocyte-like cells, and a morphologically heterogeneous third population of highly suppressive cells. The data from this study suggest that pregnant murine uteri contain a variety of cells capable of discouraging lymphocyte proliferation in vitro.     

青海高原地区胃癌患者MICA基因在mRNA及其蛋白水平表达

目的:研究青海高原地区胃癌组织中MICA基因在mRNA水平与其蛋白表达及相关关系.方法:采用RT-PCR技术检测41例高原地区胃癌组织和相应的癌旁粘膜组织中MICA mRNA表达,采用免疫组化SP法检测MICA蛋白在胃癌和癌旁组织中的表达,Spearman相关关系检验分析它们之间的相关性.结果:青海高原地区胃癌组织MICA mRNA的表达水平高于癌旁组织(P＜0.05).胃癌组织MICA蛋白表达在癌旁组织中为(26.9%,11/41)与胃癌组织阳性率为87.8%(36/41),高分化组(75.0%,6/8),中分化组(83.3%,10/12),低分化组(95.2%,20/21),之间差异有统计学意义(P＜0.05),而与肿瘤发生的性别、年龄、淋巴结转移无关(P＞0.05).Spearman相关分析显示,MICA蛋白表达水平与mRNA的表达水平呈正相关(P＜0.01).结论:MICA的过度表达在青海高原地区胃癌的发生发展过程中起着重要作用,胃癌组织中MICA mRNA以及蛋白水平的升高之间存在密切正相关.     

Ad-HGF对肺泡Ⅱ型上皮细胞的转染表达及增殖效应

目的:观察Ad-HGF对肺泡Ⅱ型上皮细胞(ATⅡ细胞)的转染表达及增殖效应.方法:以不同转染强度(50,100,200 MOI)Ad-GFP转染原代培养大鼠ATⅡ细胞,感染后48 h用流式细胞仪检测转染效率.并以最佳感染强度Ad-HGF转染ATⅡ细胞,ELISA方法检测转染后48, 72 h上清中HGF的表达水平.用MTT法检测表达产物对肺泡上皮细胞的效应.结果:感染强度为100 MOI时,对ATⅡ细胞的转染效率已达89.61%,以100 MOI的Ad-HGF转染6×105细胞后48, 72 h上清中HGF的表达分别为(249.4±1.8) ng和(168.0±26.1)ng,且表达产物对ATⅡ细胞有明显的促增殖作用.结论:Ad-HGF可有效转染ATⅡ细胞并在细胞中表达,表达产物对该细胞有明显的促增殖作用.     

携带HGF基因的减毒沙门氏菌对大鼠胃溃疡愈合及CD34表达的影响

目的:通过对乙酸性胃溃疡大鼠口服携带肝细胞生长因子(HGF)基因的减毒鼠伤寒沙门氏菌(St)后检测胃溃疡愈合情况及局部和CD34蛋白表达的研究,探讨基因药物St-HGF对乙酸性大鼠胃溃疡的治疗作用.方法:建立乙酸性胃溃疡大鼠77只,并随机选出5只,给予携带绿色荧光蛋白(GFP)基因的st后观察GFP在大鼠胃壁中的分布,剩余大鼠随机分为3组(n=24):St-HGF治疗组,St治疗组,NaHCO3对照组;增菌后各组动物于造模第2日起灌胃相应药物,隔日一次,分别于给药后7,14,21 d取胃溃疡组织行HE病理组织学观察,免疫组化法检测CD34蛋白的表达.结果:给药后7 d光镜下溃疡部位黏膜消失,局部有大量坏死组织,各组胃黏膜上皮爬行不明显;给药后14,21 d,St-HGF组黏膜上皮增生较另两组快(P<0.01);给药后7,14,21 d,CD34蛋白在St-HGF组的表达高于另两组(P<0.05),组间有统计学差异.结论:St-HGF可促进实验性大鼠胃溃疡黏膜上皮修复和溃疡面血管增生,加快溃疡愈合.     

Sinecatechins, a defined green tea extract, in the treatment of external anogenital warts: a randomized controlled trial

OBJECTIVE: To estimate the clinical efficacy of topical sinecatechins, a defined green tea extract, in the treatment of external genital and perianal warts. METHODS: This was a randomized, double-blind, vehicle-controlled trial involving 502 male and female patients aged 18 years and older, with 2-30 anogenital warts ranging from 12 to 600 mm(2) total wart area. Patients applied sinecatechins ointment 15% or 10% or vehicle (placebo) three times daily for a maximum of 16 weeks or until complete clearance of all warts, followed by a 12-week treatment-free follow-up to assess recurrence. RESULTS: Complete clearance of all baseline and newly occurring warts was obtained in 57.2% and 56.3% of patients treated with sinecatechins ointment 15% and 10%, respectively, compared with 33.7% for vehicle (both P<.001). Significance was observed at weeks 4 and 6 and all subsequent visits. Numbers needed to treat were 4.3 and 4.4. Partial clearance rates of at least 50% were reported for 78.4% and 74.0% of patients in the sinecatechins ointment 15% and 10% groups compared with 51.5% of vehicle patients. During follow-up, recurrence of any wart was observed in 6.5%, 8.3%, and 8.8% in the sinecatechins ointment 15% group, sinecatechins ointment 10% group, and vehicle patients, respectively. A total of 3.7%, 8.3%, and 0.0% developed new warts, respectively. A total of 87.7% and 87.3% of patients in the sinecatechins ointment 15% and 10% groups, and 72.1% of vehicle patients experienced application site reactions; 49.2%, 46.2%, and 65.4% of those, respectively, were mild or moderate. CONCLUSION: Topical sinecatechins ointments 15% and 10% are effective and well-tolerated in the treatment of anogenital warts. CLINICAL TRIAL REGISTRATION: ClinicalTrials.gov, www.clinicaltrials.gov, NCT00449982. LEVEL OF EVIDENCE: I.     

医学院校生物科学专业课程体系建设探讨

探讨医学生物科学专业课程构架：课程设置以医学基础知识为奠基,突显最新生命科学领域,注重综合课程,加强医学分子生物学技能训练,为社会培养生命科学领域复合、实用型人才。     

高血压致左室流出道梗阻的彩色多普勒超声诊断价值

目的：应用二维及彩色多普勒超声观察左室流出道的血流动力学变化,无创性评价高血压致左室流出道梗阻。方法：23例由于高血压导致的左室流出道梗阻患者,根据有无＂SAM＂征分为两组,无＂SAM＂征组13例,有＂SAM＂征组10例。排除肥厚型梗阻性心肌病,心肌梗死及心脏瓣膜病变。分别测量两组患者静息状态和激发试验下左室流出道血流速度及主动脉瓣上血流速度。结果：所有患者在激发试验（Valsal-va试验）后左室流出道血流速度均高于静息状态血流速度,有＂SAM＂征组静息状态和激发试验后左室流出道血流速度均高于无＂SAM＂征组,主动脉瓣上血流速度两组患者在静息状态和激发试验后差异无统计学意义。结论：超声心动图能无创、准确诊断左室流出道梗阻,激发试验能有效评价梗阻程度。     

HIE磁共振多序列分度与临床分度的一致性研究

目的探讨新生儿缺氧缺血性脑病（HIE）患儿磁共振多序列分度与临床分度的一致性。方法将经临床确诊为HIE的新生儿95例进行包括DWI及ESWAN序列的MRI多序列扫描,分别比较不同序列组合的MRI分度与临床分度的一致性。结果T1WI组MRI分度与临床分度比较Kappa值为0．32（P〈0．05）,一致性较差;T1WI＋DWI组MRI分度与临床分度比较Kappa值为0．57（P〈0．05）,一致性上升;T1WI＋DWI＋ESWAN组MRI分度与临床分度比较Kappa值为0．79（P〈0．05）,一致性好。结论T1WI联合DWI及ESWAN序列后,明显提高了HIE MRI分度与临床分度的一致性。