超临界连续GAS过程制备对苯二酚超细颗粒

针对传统RESS过程中存在着颗粒质量和产量问题,本文分别以超临界CO2作为溶剂在萘的固态和熔融状态下利用RESS过程制备了萘的超细颗粒,研究了萘在固态和熔融态下各种操作变量,如萃取温度和压力、膨胀前温度和喷嘴尺寸等对产品尺寸的影响。结果表明,膨胀前温度和萃取压力的作用规律相同,提高膨胀前温度则颗粒变大,提高萃取温度,颗粒尺寸变小;而在熔融状态下反之,在该研究范围内,喷嘴尺寸的熔融态和固态下对颗粒尺寸影响不大。     

气固法合成氯磺化聚乙烯——Ⅰ.气—固相反应机理

以氯化聚乙烯为原料,氯气和二氧化硫为反应气体,紫外光引发,气固法合成了氯磺化聚乙烯。通过元素分析得出,氯磺化反应中不存在氯化和脱氯的副反应。在釜式反应器内研究了氯磺化反应的气固相反应机理,发现气体的内扩散和本征化学反应是氯磺化反应的控制步骤,升高温度（在30－50℃范围内）使氯磺化反应后期的速率显著提高,这是由氯化聚乙烯的共结晶的熔融引起的。还讨论了氯化聚乙烯的链结构和固态结构对氯磺化反应的影响。     

云南琵琶甲抗菌活性成分研究

目的：研究云南琵琶甲的抗菌活性成分。方法：通过溶剂提取、硅胶柱层析分离,应用光谱方法鉴定结构。结果：所分得的5个化合物分别鉴定为2－甲基－1,4苯二醇（2-methyl-1,4-benzenediol,Ⅰ）,对苯二酚（hydroquinone,Ⅱ）,三十二烷酸（dotriacontanoic acid),棕榈酸（palmitic acid,Ⅳ）,和三十烷醇（triacontanol,Ⅴ）,经抗菌活性实验,结果表明Ⅰ和Ⅱ具有抗菌活性。结论：以上5个化合物为首次从云南琵琶甲中分得。     

天麻的组织培养及快速繁殖

目的：利用组织培养方法快速繁殖成米麻作为种麻,为寻找出一简便可行的快繁天麻种麻的新途径提供理论依据。方法：天麻块茎或茎尖无菌离体培养,米麻块茎诱导培养基为1／2MS＋6－BA 1mg/L NAA0.5mg/L＋香蕉50mg/L;箭麻茎尖诱导培养基为1／2MS＋6－BA2mg/L NAAO.2mg/L。结果：小米麻经50d无菌培养后开始生长出新米麻,70d后原小米麻块茎上生长出多个分枝的新米麻;箭麻茎尖超过140d组织培养,茎尖分化、诱导形成原球茎,超过160d培养原球长大并开花。结论：天麻可以用组织培养方法快速繁殖成米麻作为种麻。     

端粒酶及nm23的表达与胃腺癌预后的关系探讨

目的 :探讨端粒酶和nm2 3癌基因蛋白表达与胃癌根治术后复发及预后的关系。方法 :采用端粒酶原位分子杂交技术及免疫组化S P法分别对 4 5例原发性胃腺癌组织中端粒酶的活性及nm2 3进行检测 ,并结合内镜及随访资料进行分析。结果 :胃腺癌端粒酶阳性表达率及nm2 3低表达率在有淋巴结转移组显著高于无淋巴结转移组 ;在术后 5年内复发者中显著高于无复发者。虽然随着胃腺癌分化程度的降低及浸润深度的增加 ,端粒酶活性及nm2 3低表达呈增强趋势 ,但其差异无统计学意义。结论 :胃腺癌组织中端粒酶的活性表达及nm2 3的低表达与淋巴结转移及术后复发显著相关 ,两者的表达变化对判断胃癌术后复发及预后判断有重要临床意义     

胃癌中P16与MDM2的免疫组化研究

目的 :检测胃肿瘤及正常胃组织中P16与MDM2的表达 ,探讨其在肿瘤凋亡中的相互关系。方法 :采用免疫组织化学方法 (SABC法 )。结果 :胃癌中P16阳性率为 69% ,MDM2阳性率为 75 % ,它们与正常胃组织及良性胃肿瘤相比差异均有显著性 (P <0 .0 5 )。结论 :在胃癌的细胞凋亡中 ,P16与MDM2均发挥了其各自对细胞凋亡的作用 ,为临床胃癌的组织学检测提供了理论依据 ,同时也支持胃癌的发生是多基因协同作用的过程     

血压、血脂、血糖与心脑血管疾病相关性研究

目的 :为了解高血压、高血脂、高血糖在心脑血管疾病的发病方面所起的作用及影响程度的相关性。方法 :对武汉市 3 15 1例脑力劳动者健康体检资料进行分析。结果 :血糖、血脂均值及高血压、糖尿病、心脑血管疾病的患病率都随年龄的增长而增高 ,高血压与心脑血管疾病在患病率增高方面有明显的相关性 ,而血脂、血糖水平与心脑血管疾病的患病率之间相关性却不甚明显。结论 :脑力劳动者患高血压病 ,特别是长期药物控制不好的高血压病才是心脑血管疾病发病的最重要危险因素 ,降低心脑血管疾病的发病率和复发率的关键是 ,加强对高血压病的监控 ,合理用药 ,稳定血压     

Effect of Fuzheng Kangbai Granule (扶正抗白冲剂) on Immune Function and Survival Time in Minimal Residual Leukemia Model Mice

Ｍｉｎｉｍａｌｒｅｓｉｄｕａｌｌｅｕｋｅｍｉａ（ＭＲＬ）ｉｓｔｈｅｏｒｉｇｉｎｏｆｌｅｕｋｅｍｉａｒｅｌａｐｓｅ,ｔｈｅｔｒｅａｔｍｅｎｔｏｆＭＲＬｉｓｔｈｅｋｅｙ ｐｒｏｂｌｅｍｉｎ ｐｒｅｖｅｎｔｉｎｇｌｅｕｋｅｍｉａｒｅｌａｐｓｅａｎｄｐｒｏｌｏｎｇｉｎｇｔｈｅｉｌｌｎｅｓｓ ｆｒｅｅｓｕｒｖｉｖａｌｏｆｌｅｕｋｅｍｉａｐａｔｉｅｎｔｓ．ＴＣＭｈｏｌｄｓｔｈａｔｔｈｅｍａｎｉｆｅｓｔａｔｉｏｎｏｆＭＲＬｃｏｎｓｉｓｔｅｄｏｆｔｗｏｃｈａｒａｃｔｅｒｉｓｔｉｃｓ：ｔｈｅ Ｅｖｉｌｅｌｉｍｉｎａ…     

Osteogenic potential of cultured bone marrow stromal cells transfected with transforming growth factor β1 genein vitro

Summary To study the osteogenic potential of cultured bone marrow stromal cells (BMSCs) transfected with transforming growth factor β₁ (TGF-β₁) genein vitro, cultured BMSCs were transfected with the complexes of pcDNA₃-TGF-β₁ and Lipofectamine Reagentin vitro. The cell proliferation was detected by MTT method and the morphological features of transfected BMSCs was observed. ALP stains and PNP method were used to measure ALP activity. In addition, the collagen type I propeptides and mineralized matrixes were examined by immunohistochemical staining and tetracycline fluorescence labeling respectively. The morphological and biological characters of the transfected BMSCs were similar to those of osteoblasts and the cell proliferation was promoted. The cell layer displayed strong positive reaction for ALP stains and immunohistochemical staining. ALP activity and collagen type I expression increased remarkably after transfection. Mineralized matrixes formed earlier and more in transfected BMSCs as compared with control group. It is concluded that transfecting with TGF-β₁ gene could promote the osteogenic potential of cultured BMSCs.     

Molecular Tissue Engineering: Applications for Modulation of Mesenchymal Stem Cells Proliferation by Transforming Growth Factor β_1 Gene Transfer 

It is well established that damaged articularcartilage has only a limited ability to healitself. Tissueengineering represents a new alternative approach tocartilage repairing. However,as it is very difficult toacquire seed cells with good activity,especially thoseinflammatory factors which induce the degradation ofarticular cartilage in the pathology of trauma orosteoarthritis were still exited,the success of all ofthese techniques has been variable and limited in thatnone were able to restore…