正畸远移尖牙方式与龈沟液RANKL／OPG表达比值的相关性研究

目的探讨不同加力方式对牙齿移动速度的影响以及与龈沟液核因子κB受体活化子配体／骨保护素表达比值（RANKL／OPG）的相关性。方法选取双侧对称拔除4颗第一双尖牙的恒牙列患者,随机分为螺旋弹簧施力组和弹力橡皮链施力组,实施尖牙远中移动,分别于加力前,加力2、4、8、12、16、20周测量尖牙移动量和龈沟液RANKL／OPG值。结果加力4周后,螺旋弹簧组每个时间点的尖牙移动量均大于橡皮链组。两组各加力时间点的RANKL／OPG值均有显著差异。尖牙移动到位后和稳定3个月后的定位根尖片均无牙根吸收现象。结论螺旋弹簧组的骨改建作用强于橡皮链组,龈沟液RANKL／OPG值与骨改建过程具有相关性。     

高效液相色谱-荧光法测定丙泊酚的血药浓度

目的:建立高效液相色谱-荧光法检测丙泊酚血药浓度的方法。方法:采用饱和硫酸铵溶液作蛋白沉淀剂,以乙腈-水(70:30,含0.1%三氟乙酸)为流动相,流速1.0ml·min-1,荧光检测器检测。结果:丙泊酚血药浓度范围为0.1～10.0mg·L-1,(r=0.999 5);最低检测限为0.02mg·L-1;平均提取回收率为87.03%,RSD为2.54%;平均方法回收率为105.08%,RSD为3.81%;日内差异RSD为4.31%;日间差异RSD为7.54%。结论:本检测方法操作简便、灵敏度高、专一性强、结果可靠,可用于丙泊酚血药浓度监测和药物代谢动力学研究。     

环境侵权投诉中健康损害判定程序研究

提出环境污染健康损害判定工作程序及步骤,给出已知污染物或未知污染物造成健康损害判定工作框图,并提出健康损害判定:①必须经环境保护主管部门进行资质认定的环境污染健康损害判定机构承担;②具有健康损害判定资质的机构应建立相应专家组;③健康损害判定专家组按相关标准根据医学诊断、环境证据及因果关系进行健康损害判定。     

顶空气相色谱法分析生活饮用水中挥发性卤代烃类有机物的方法探讨与应用

目的:探讨生活饮用水中7种挥发性卤代烃的测定方法。方法:采用顶空气相色谱法测定生活饮用水中三氯甲烷、四氯化碳、二氯一溴甲烷、一氯二溴甲烷、三氯乙烯、四氯乙烯及溴仿。结果:在所建立的实验条件下,7种挥发性卤代烃的平均回收率在91.6%~104.7%之间,相对标准差为1.2%~7.3%,检测限为0.20~5.0μg/L。结论:所建立的分析方法简便、快捷、准确、可同时测定多种挥发性卤代烃。     

rhEPO、rhG-CSF对芥子气全身吸收中毒犬疗效观察

目的　评价重组人红细胞生成素 (rhEPO)、重组人粒细胞集落刺激因子 (rhG CSF)对芥子气全身吸收中毒犬的治疗效果。方法　以成年家犬为中毒模型 ,共 10条 ,中毒剂量为 12mg kg,皮下注射。随机分为中毒对照组 4条 ,治疗组 6条 ,治疗组动物在中毒后 30min即进行相应的药物治疗。中毒前所有犬各测一次血常规的基础值 ,中毒后每日监测血常规的动态变化 ,持续 1周。结果　对照组犬于 3天内死亡 3条 ,治疗组犬全部成活。对照组白细胞计数 (WBC)在中毒后第 3开始剧烈下降 ,红细胞计数 (RBC)在中毒后即有轻度上升 ,之后回落 ;而治疗组WBC、RBC在中毒后 1周内均无显著变化。两组的淋巴细胞计数 (LYM)在中毒后都有显著下降 ,治疗组稍好于对照组。治疗组的网织红细胞计数 (RC)于中毒后第 2天开始显著增长 ,之后维持在高水平 1周左右。治疗组的RC变化不明显。结论　犬芥子气中毒后 ,应用rhEPO、rhG CSF能明显改善中毒犬的部分血常规指标     

138例食管胃结合部肿瘤病例分析

目的：通过分析138例食管胃结合部肿瘤的临床病理特征、手术方式,探讨食管胃结合部肿瘤不同Siewert分型和手术方式对预后的影响。方法：回顾性分析洛阳市中心医院2000年-2006年食管胃结合部肿瘤138例手术病例的临床病理特征及手术方式,将138例病例参照Siewert分类方法进行分类,分析不同Siewert分型和手术方式对预后的影响。结果：138例中AEGⅠ型10例,AEGⅡ型20例,AEGⅢ型108例,三种类型AEG预后存在明显差别,不同手术方式的5年生存率明显不同,脾切除的患者生存时间无明显改善,并发症增加。结论：三种类型的AEG预后不同,选择不同手术方式其5年生存率亦不同,根据AEG分型选择适当手术方式可以改善预后;脾脏切除并不能改善患者预后,保留脾脏的淋巴结清扫是可行的,但要确保做到肿瘤的根治切除和严格的淋巴结清扫。     

pH and Redox-Dual Sensitive Chitosan Nanoparticles Having Methyl Ester and Disulfide Linkages for Drug Targeting against Cholangiocarcinoma Cells

The aim of this study is to prepare pH- and redox-sensitive nanoparticles for doxorubicin (DOX) delivery against DOX-resistant HuCC-T1 human cholangiocarcinoma (CCA) cells. For this purpose, L-histidine methyl ester (HIS) was attached to chitosan oligosaccharide (COS) via dithiodipropionic acid (abbreviated as ChitoHISss). DOX-incorporated nanoparticles of ChitoHISss conjugates were fabricated by a dialysis procedure. DOX-resistant HuCC-T1 cells were prepared by repetitive exposure of HuCC-T1 cells to DOX. ChitoHISss nanoparticles showed spherical morphology with a small diameter of less than 200 nm. The acid pH and glutathione (GSH) addition induced changes in the size distribution pattern of ChitoHISss nanoparticles from a narrow/monomodal distribution pattern to a wide/multimodal pattern and increased the fluorescence intensity of the nanoparticle solution. These results indicate that a physicochemical transition of nanoparticles can occur in an acidic pH or redox state. The more acidic the pH or the higher the GSH concentration the higher the drug release rate was, indicating that an acidic environment or higher redox states accelerated drug release from ChitoHISss nanoparticles. Whereas free DOX showed decreased anticancer activity at DOX-resistant HuCC-T1 cells, DOX-incorporated ChitoHISss nanoparticles showed dose-dependent anticancer activity. Intracellular delivery of DOX-incorporated ChitoHISss nanoparticles was relatively increased at an acidic pH and in the presence of GSH, indicating that DOX-incorporated ChitoHISss nanoparticles have superior acidic pH- and redox-sensitive behavior. In an in vivo tumor xenograft model, DOX-incorporated ChitoHISss nanoparticles were specifically delivered to tumor tissues and then efficiently inhibited tumor growth. We suggest that ChitoHISss nanoparticles are a promising candidate for treatment of CCA.     

对苯二胺氧化产物对小鼠经皮肤染毒的亚慢性毒性

[目的 ]研究氧化型染发剂主要成分对苯二胺 (PPD)氧化产物经皮吸收后对小鼠的亚慢性毒作用。 [方法 ]对苯二胺经双氧水氧化后的氧化产物为受试物 ,每隔 3d在小鼠背部皮肤染毒 1次 ,周期 1个月。整个过程中观察小鼠的一般生长情况 ,并于染毒 1个月后观察受试动物血生化指标、血常规、肝肾脾组织病理学改变和遗传毒性。 [结果 ]PPD氧化产物可影响小鼠的肝肾功能 ,随着PPD染毒剂量增加 ,ALT和BUN值逐渐增高 ,与对照组比较差异有显著性 (P<0 0 5或P <0 0 1)。PPD具有遗传毒性 ,能造成骨髓细胞染色体和DNA的损伤 ,并在受试剂量范围内存在剂量 效应关系。 [结论 ]频繁使用氧化型染发剂可能对机体具有潜在的危害     

石墨炉原子吸收法直接测定血清中硒的方法研究

目的:建立测定血清中硒的新方法。方法:应用Triton X-100稀释样品,横向塞曼石墨炉原子吸收光谱法直接测定样品中痕量硒。结果:选定了最佳测量条件,该方法的灵敏度、精密度均能满足检测工作的需要。本法对硒的最低检出量为14.7 pg,回收率在90%~102%之间。结论:本法简便、快速、准确,灵敏度和精密度高,适用于血清中硒的测定。     

RNA Interference-Mediated Knockdown of Bombyx mori Haemocyte-Specific Cathepsin L (Cat L)-Like Cysteine Protease Gene Increases Bacillus thuringiensis kurstaki Toxicity and Reproduction in Insect Cadavers

The silkworm’s Cat L-like gene, which encodes a lysosomal cathepsin L-like cysteine protease, is thought to be part of the insect’s innate immunity via an as-yet-undetermined mechanism. Assuming that the primary function of Cat L-like is microbial degradation in mature phagosomes, we hypothesise that the suppression of the Cat L-like gene expression would increase Bacillus thuringiensis (Bt) bacteraemia and toxicity in knockdown insects. Here, we performed a functional analysis of Cat L-like in larvae that were fed mulberry leaves contaminated with a commercial biopesticide formulation based on Bt kurstaki (Btk) (i.e., Dipel) to investigate its role in insect defence against a known entomopathogen. Exposure to sublethal doses of Dipel resulted in overexpression of the Cat L-like gene in insect haemolymph 24 and 48 h after exposure. RNA interference (RNAi)-mediated suppression of Cat L-like expression significantly increased the toxicity of Dipel to exposed larvae. Moreover, Btk replication was higher in RNAi insects, suggesting that Cat L-like cathepsin may be involved in a bacterial killing mechanism of haemocytes. Finally, our results confirm that Cat L-like protease is part of the antimicrobial defence of insects and suggest that it could be used as a target to increase the insecticidal efficacy of Bt-based biopesticides.