电针、中药促进大鼠坐骨神经损伤的神经再生研究

目的：寻找促进周围神经损伤后神经再生的方法。方法：采用手术造成大鼠坐骨神经损伤模型，用电针、中药治疗，进行电生理指标和HRP追踪观察。结果：电针组、中药组的神经传导速度和诱发动作电位振幅恢复率以及脊髓前角和脊神经节标记细胞数，与西药组、空白组比较差异有显著性意义，其中电针组优于中药组。结论：电针、中药均能较好地促进神经损伤早期的功能恢复，是一种促进周围神经损伤后神经再生的有效手段。     

中药含药血清对体外培养鼠破骨细胞的影响

目的 :研究骨康抑制破骨细胞性骨吸收的机理。方法 :新鲜牛皮质骨经锯式切片机横切成 5 0um厚0 .5cm× 0 .5cm大小骨片 ,参照已建立的破骨细胞分离培养方法 ,从新生SD大鼠四肢长骨分离破骨细胞。原代破骨细胞培养 2 4小时后 ,加入含药血清培养 7天。结果 :发现培养的细胞具有典型破骨细胞的形态特点。结果可见破骨细胞能在骨片表面形成吸收陷窝 ,其形态多样 ,随时间延长 ,陷窝扩大 ,数量增多 ,而骨康含药血清可抑制骨陷窝数量。结论 :这说明含药血清可直接抑制破骨细胞的功能 ,骨康抑制骨吸收的功能可能与其直接抑制破骨细胞功能有关。     

刺五加叶皂甙单体Sb对培养大鼠心肌细胞动作电位的影响

本研究中所用的刺五加叶皂甙单体Ｓｂ（５０、２００μｇ／ｍＬ），使培养的ｗｉｓｔａｒ大鼠心肌细胞动作电位的波幅、波宽、阈电位、最大舒张电位、超射、最大除极速度及复极（１０％、５０％、９０％）水平的动作电位波宽一致减小。Ｃａ２＋８０μｇ／ｍＬ能使之反转，Ｓｂ作用与尼莫地平作用相似。上述结果表明Ｓｂ具有钙通道阻滞作用。     

原发性高血压的分离分析

用Ｍｏｒｔｏｎ的不完全确认条件下的分离分析方法，对１４１个原发性高血压核心家庭进 行假设检验及最大似然估计，在ＩＢＭ微机上进行计算。结果表明、根据不同病例来源所分的总体、 医院、群体３组资料中，Ａ×Ｕ和Ｕ×Ｕ婚配型都接受显性遗传分离比的假设，分离比依次为 ０．２８６、０．２６０、０．３０５，外显率依次为０．５７２、０．５２０、０．６１０。Ｕ×Ｕ婚配型中，存在散发病例。ＵＸＵ多 发家庭中，不排斥Ｐ＝０．５的假设。提示原发性高血压有常染色体显性遗传不完全外显的倾向。     

妇癌患者的社会心理因素调查

本文以自编病前负性情绪调查表、ＬＳＥ─Ｒ及ＥＰＱ对５６例妇癌住院病人进行了调查，并以５６例内科病住院病人作对照。结果显示，６９．６％的妇癌病人在病前有负性情绪，而内科病病人只有１６．１％；８５．７％的妇癌病人病前经历了负性生活事件，而内科病组则为５８．９％；ＥＰＱ评定结果神经质分亦为前者高于后者，均提示社会心理因素与妇癌的发生密切相关。     

荧光法测定小鼠体内各组织中环丙沙星的浓度

目的:测定小鼠体内各组织中环丙沙星的浓度。方法:采用三氯化铝络合环丙沙星-荧光分光光度法。结果:在λ_(ex)=330 nm,λ_(em)=440 nm处,络合物的荧光值不受各组织的影响,且比环丙沙星自身的荧光值增加了20倍,线性范围为50～1000ng·mL~(-1)。结论:此方法准确、快速,适用于环丙沙星的体内研究。     

Regulation on maturation and function of dendritic cells by Astragalus mongholicus polysaccharides

Astragalus mongholicus polysaccharides(ASP) isolated from one of the Chinese herbs-A. mongholicus which are known to have a variety of immunomodulatory activity. However, little is known about their immunomodulatory effects on murine bone marrow (BM)-derived dendritic cells (DC). DC are professional antigen presenting cells, which are pivotal for initiation of primary immune response. In this study, the regulatory effects of ASP on maturation and function of cultured murine BM-derived DC were investigated in vitro. ASP (10, 50, 100, 250 microg/ml) could increase the co-expression of CD-11c and MHC class II molecules on DC surface, and the 100 microg/ml is the optimal dose. The ability of unstimulated DC to uptake FITC-dextran was higher than that of ASP- or LPS-treated DC. We analyzed the concentration of IL-12 secreted by DC using ELISA. ASP-treated DC secreted a higher level of IL-12 than untreated DC. And ASP- or LPS-treated DC displayed a more mature morphology, with long protrusions, while untreated-DC displayed shorter protrusions than stimulated DC.     

Headspace solvent microextraction-gas chromatography-mass spectrometry for the analysis of volatile compounds from Foeniculum vulgare Mill

A novel and rapid headspace solvent microextraction followed by gas chromatography-mass spectrometry (HSME-GC-MS) for the analysis of the volatile compounds of Foeniculum vulgare Mill is described. HSME parameters including extracting solvent, extraction temperature and time, headspace volume and particle size were optimized. As a result, benzyl alcohol was finally used for the extraction at 70 degrees C for 20 min with headspace volume of 12.1 ml and particle size of 120 mesh. Under the determined conditions, the powered samples of Foeniculum vulgare Mill were directly applied for the analysis. A comparison of HSME-GC-MS, solid phase microextraction (SPME)-GC-MS and steam distillation (SD)-GC-MS methods was made and showed that the HSME-GC-MS method was simple, inexpensive and effective and can be used for the analysis of volatile compounds in traditional Chinese medicines (TCMs).     

脑瘫舒筋活络按摩油在痉挛型脑瘫患儿推拿中的应用

目的:探讨脑瘫舒筋活络按摩油对痉挛型脑瘫患儿推拿治疗的辅助作用。方法:对262例痉挛型脑瘫患儿应用脑瘫舒筋活络按摩油进行推拿治疗,并在治疗前后进行综合评价,进行统计学处理。结果:262例痉挛型脑瘫患儿经过3个小疗程治疗,肌张力显效98例(占37.40%),有效155例(占59.16%),无效9例(占3.44%);关节活动度接近或恢复正常范围者占96.56%,且配合程度越好,治疗效果越显著。结论:应用脑瘫舒筋活络按摩油进行婴幼儿推拿治疗,既能滋润保护皮肤黏膜,使患儿舒适配合,起到介质作用,又能有效地缓解痉挛,降低肌张力,改善关节活动度。     

建德市肾综合征出血热Ⅰ型灭活疫苗随机对照现场中对照组人群隐性感染定群研究

目的：用定群血清流行病学方法研究ＨＦＲＳ疫苗效果考核现场对照组人群隐性感染情况及感染后抗体水平的变化。方法：采用ＩＦＡＴ检测血清中ＨＦＲＳＩｇＧ抗体,分析不同性别和年龄的隐性感染及感染后抗体水平变化情况。结果：对照组３６０人随访４０个月（１９９４年７月至１９９７年１１月）,ＨＦＲＳ年感染率２９３％,不同性别和年龄组未见显著差异;３２名隐性感染者ＨＦＲＳ抗体转阴率４６８８％,也可见ＨＦＲＳ抗体上升率达２５００％,在不同ＨＦＲＳ抗体滴度、性别和年龄组中未见显著差异。结论：此研究结果将为疫苗现场效果评价提供一些背景资料