Casticin prevents DSS induced ulcerative colitis in mice through inhibitions of NF‐κB pathway and ROS signaling

Casticin, a compound purified from the Chinese herb Viticis Fructus, has been proven effective in preventing tumor progression in previous studies. Ulcerative colitis (UC) is a common inflammatory bowel disease that affects millions of people worldwide, but no effective and safe drugs are available. In this study, we aimed to study how did casticin affect UC by evaluating its effects on dextran sulfate sodium (DSS)‐induced colitis in mice. Our data suggested that casticin attenuated body weight loss, colon length shortening, and pathological damage in the colon of DSS‐treated mice. Casticin decreased reactive oxygen species level and chemocytokines (IL‐1β, IL‐6, TNF‐α) productions in colon tissue. The decreased reactive oxygen species level and suppressed proinflammatory cytokines productions were also confirmed in casticin‐treated LPS‐stimulated RAW264.7 cells and hydrogen peroxide‐treated CACO‐2 cells in vitro. Mechanistically, casticin treatment prevented the profound activation of AKT signaling caused by DSS administration. And casticin inhibited the productions of proinflammatory chemocytokines through downregulating AKT/NF‐κB pathway in macrophages. Meanwhile, data revealed that casticin increased expressions of endogenous antioxidants peroxiredoxin 3 and MnSOD were through activation in FOXO3α signaling by downregulating AKT signaling in colon epithelium cells. Our findings demonstrated that casticin alleviated DSS‐induced UC by increasing the antioxidant enzyme peroxiredoxin 3 and MnSOD expressions, and decreasing the production of proinflammatory chemocytokines through inhibition of AKT signaling.     

麝香酮对氰化钠加缺糖致PC12细胞缺血损伤的保护作用

目的:研究麝香酮对PC12细胞缺血损伤的影响。方法:在离体培养的PC12细胞,用NaCN加缺糖造成拟缺血损伤模型,通过细胞形态学观察、MTT微量比色、培养介质LDH活力测定,研究了麝香酮对该模型的保护作用。结果:10-7~510-5mol/L范围内,麝香酮呈浓度依赖性地降低NaCN加缺糖造成拟缺血损伤模型培养介质内LDH的释放,其IC50为:43.33μmol/L。在10-7~510-5mol/L范围内,麝香酮呈浓度依赖性地增加NaCN加缺糖造成拟缺血损伤模型培养介质的MTT比色值,其IC50为30.62μmol/L。结论:麝香酮对PC12细胞缺血损伤具有保护作用。     

肿瘤细胞与血管内皮细胞相互作用及丹参干预作用

肿瘤转移的复杂机制在于肿瘤细胞与各种宿主细胞之间发生的广泛作用,而肿瘤能否建立转移则取决于肿瘤细胞诱导并利用宿主细胞协助自身发展的能力。其中肿瘤与血管内皮细胞间的相互作用在支持肿瘤血行转移中扮演了重要角色。研究表明活血化瘀药丹参对这一过程具有多成分、多环节的抑制作用,为以肿瘤细胞与血管内皮细胞相互作用为靶点的抗肿瘤候选药物研发提供参考。     

冬虫夏草子囊孢子的弹射

在四川康定和西藏那曲两地,对虫草子座的光照强度处理,结果表明：两地的虫草子囊孢子弹射时间有差异;光照强度不同,子囊孢子弹射的数量,时间和萌发率均不同。要使冬虫夏草子囊孢子正常弹射和提高萌发率,必须满足温度、湿度和日照三个基本条件。本结果为人工培植冬虫夏草奠定基础。     

宁心痛颗粒含药血清对THP-1源性巨噬细胞TLR4、Syk、ERK、Paxillin蛋白磷酸化水平与细胞吞噬功能的影响

目的观察宁心痛颗粒含药血清对"轻度氧化修饰低密度脂蛋白-Toll样受体4-巨噬细胞"途径的干预作用,探讨其稳定动脉粥样硬化(Atherosclerosis,AS)斑块、抑制AS进展的分子机制。方法将THP-1源性巨噬细胞分为空白组、模型组、空白血清组、宁心痛颗粒含药血清组4组。采用免疫印迹法检测脾酪氨酸激酶(spleen tyrosine kinase,Syk)、细胞外信号调节激酶(extracellular regulated protein kinases,ERK1/2)、桩蛋白(Paxillin)磷酸化水平随时间变化情况,以及宁心痛颗粒含药血清对Syk、ERK、Paxillin蛋白磷酸化水平的影响;采用免疫沉淀联合免疫印迹法检测Toll样受体4(Toll like receptor 4,TLR4)蛋白磷酸化水平随时间变化情况,以及宁心痛颗粒含药血清对TLR4蛋白磷酸化水平的影响;采用中性红吞噬实验检测巨噬细胞吞噬功能改变。结果 TLR4、Syk、ERK、Paxillin分别于15,10,15,30min达到磷酸化水平高峰;与空白组相比,模型组及空白血清组TLR4、Syk、ERK、Paxillin磷酸化水平升高、细胞吞噬功能增强(P0.05);与模型组和空白血清组相比,含药血清组TLR4、Syk、ERK、Paxillin磷酸化水平降低、细胞吞噬功能减弱(P0.05),但仍高于空白组。结论宁心痛颗粒含药血清可能通过"mm LDL-TLR4-巨噬细胞"途径影响巨噬细胞吞噬功能从而具有稳定AS斑块、干预AS作用。     

增殖性糖尿病视网膜病变多次手术临床分析

目的：探讨增殖性糖尿病视网膜病变玻璃体切割术后多次手术的原因及处理。方法：回顾性分析3a来因增殖性糖尿病视网膜病变行玻璃体切割术后需要再次手术的患者。结果：患者189眼术后需要再次进行手术的为24例26眼（占总观察眼数13.8%）。分别是：玻璃体再积血3眼,前房积血1眼,视网膜脱离11眼（占总观察眼数5.8%,占再手术眼42.3%,其中3眼合并新生血管性青光眼）,白内障6眼（占总观察眼数3.2%）,单纯硅油取出5眼。189眼中有6眼发生了新生血管性青光眼（3.2%）,6眼均为玻璃体晶状体联合手术或玻璃体切割术后又摘除白内障病例。2次手术19眼,3次或以上手术7眼（其中5眼合并视网膜脱离）。结论：发生视网膜脱离是糖尿病视网膜病变玻璃体切割术后需要多次手术的主要原因;也占据需要3次或以上手术的主要部分。新生血管性青光眼的发生值得重视,出现视网膜脱离时要提高警惕,需要摘除晶状体时必须慎重。     

蓝-黄视野计与标准的白-白视野计对早期原发性开角型青光眼视野的对比研究

目的　评价蓝 -黄视野计 (blue- on- yellow perim etry,B/ Y)又称短波长视野计 (short- wavelength auto-mated perim etry,SWAP)与标准的白色视野计 (white- on- white perimetry,W/ W)在早期青光眼视野损害诊断中的意义。方法　利用 Hum phrey- - 75 0型全自动视野计 C- 30 - 2全阈值检测程序对 2 0例 (4 0只眼 )正常人、2 0例 (36只眼 )可疑青光眼、18例 (32只眼 )确诊的早期原发性开角型青光眼 (primary open- angle glaucoma,POAG)分别进行 W/ W与 B/ Y的视野检查。结果　正常人、可疑青光眼、早期青光眼 B/ Y检测的全视网膜光敏感度均值 (MS)低于 W/ W检测的结果 ,两者有显著性意义 (t=11.6 8,P <0 .0 1;t=14 .0 1,P <0 .0 1;t=14 .6 8,P <0 .0 1)。B/ Y检测光敏感度缺损均值 (MD)与 W/ W检测的结果 ,正常人组无显著性意义 (t=1.0 4 ,P <0 .0 1) ,其它两组有显著性意义 (t=4 .88,P <0 .0 1;t=3.378,P <0 .0 1)。W/ W检查正常人组与可疑性青光眼的 MS差别无显著意义 (t=2 .5 4 ,P >0 .0 1) ,B/ Y有显著意义 (t=5 .5 7,P <0 .0 1)。两组的 MD差别均有显著意义 (t=3.16 ,P <0 .0 1,t=6 .2 6 ,P <0 .0 1) ;W/ W与 B/ Y检查正常人组与早期青光眼组的 MS差别有显著意义 (t=6 .4 7,P <0 .0 ;t=10 .19,P     

宫颈鳞癌中Cyclin E的表达和HPV16感染的相关研究

目的探讨Cyclin E在宫颈鳞状细胞癌中的表达及其与HPV16感染的关系.方法应用免疫组化S-P法对34例宫颈鳞状细胞癌组织进行Cyclin E与HPV16的检测,并与宫颈不典型增生和正常宫颈组织进行比较.结果正常宫颈组织无Cyclin E表达,宫颈不典型增生组织仅有少量袁达(11.11%),宫颈鳞状细胞癌有58.52%表达(P＜0.001),且与HPV16感染有明显相关性(P＜0.05).结论高危HPV感染是宫颈癌的重要病因,HPV感染通过多种途径改变了细胞的周期性调控,诱发细胞周期GI-S期检测点异常,引起细胞恶性增生,HPV感染与Cyclin E异常表达在宫颈癌的发生发展中起重要作用.     

The role of electrochemical biosensors in SARS-CoV-2 detection: a bibliometrics-based analysis and review

The global pandemic of COVID-19, which began in late 2019, has resulted in extremely high morbidity and severe mortality worldwide, with important implications for human health, international trade, and national politics. Severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the primary pathogen causing COVID-19. Analytical chemistry played an important role in this global epidemic event, and detection of SARS-CoV-2 even became a part of daily life. Analytical chemists have devoted much effort and enthusiasm to this event, and different analytical techniques have shown very rapid development. Electrochemical biosensors are highly efficient, sensitive, and cost-effective and have been used to detect many highly pathogenic viruses long before this event. However, another fact is that electrochemical biosensors are not the technology of choice for most detection applications. This review describes for the first time the role played by electrochemical biosensors in SARS-CoV-2 detection from a bibliometric perspective. This paper analyzed 254 relevant research papers up to June 2022. The contributions of different countries and institutions to this topic were analyzed. Keyword analysis was used to explore different methodological attempts of electrochemical detection techniques. More importantly, we are trying to find an answer to the question: do electrochemical biosensors have the potential to become a genuinely employable detection technology in an outbreak of infectious disease?

This review describes for the first time the role played by electrochemical biosensors in SARS-CoV-2 detection from a bibliometric perspective.     

双黄连注射剂不良反应的原因及分析

目的观察、分析和总结双黄连注射剂的不良反应和应对措施。方法通过查阅1994～2008年期间国内公开发行的医药卫生期刊和国家食品药品监督管理局药品不良反应监测中心《信息通报》有关药品不良反应的资料进行分析。结果11083例双黄连注射剂不良反应的病例报告中出现严重不良反应的有306例,涉及158家企业的产品。性别、年龄段没有明显差异;以用药当天出现不良反应为主,严重不良反应的治愈或好转率〉90%,总死亡率高达5.9%,大多数病例对疾病本身影响不大。结论应根据双黄连注射液出现不良反应的可能原因,采取有针对性的安全防治措施。