全文获取类型
收费全文 | 10189篇 |
免费 | 480篇 |
国内免费 | 67篇 |
专业分类
耳鼻咽喉 | 118篇 |
儿科学 | 173篇 |
妇产科学 | 146篇 |
基础医学 | 1226篇 |
口腔科学 | 334篇 |
临床医学 | 774篇 |
内科学 | 2477篇 |
皮肤病学 | 171篇 |
神经病学 | 968篇 |
特种医学 | 266篇 |
外科学 | 1731篇 |
综合类 | 56篇 |
一般理论 | 1篇 |
预防医学 | 381篇 |
眼科学 | 281篇 |
药学 | 798篇 |
中国医学 | 36篇 |
肿瘤学 | 799篇 |
出版年
2022年 | 79篇 |
2021年 | 180篇 |
2020年 | 99篇 |
2019年 | 129篇 |
2018年 | 173篇 |
2017年 | 147篇 |
2016年 | 178篇 |
2015年 | 181篇 |
2014年 | 194篇 |
2013年 | 228篇 |
2012年 | 418篇 |
2011年 | 486篇 |
2010年 | 324篇 |
2009年 | 244篇 |
2008年 | 399篇 |
2007年 | 456篇 |
2006年 | 460篇 |
2005年 | 455篇 |
2004年 | 440篇 |
2003年 | 449篇 |
2002年 | 431篇 |
2001年 | 385篇 |
2000年 | 337篇 |
1999年 | 340篇 |
1998年 | 134篇 |
1997年 | 96篇 |
1996年 | 103篇 |
1995年 | 74篇 |
1994年 | 78篇 |
1993年 | 91篇 |
1992年 | 275篇 |
1991年 | 212篇 |
1990年 | 193篇 |
1989年 | 218篇 |
1988年 | 185篇 |
1987年 | 221篇 |
1986年 | 183篇 |
1985年 | 181篇 |
1984年 | 130篇 |
1983年 | 110篇 |
1979年 | 119篇 |
1978年 | 61篇 |
1975年 | 57篇 |
1974年 | 67篇 |
1973年 | 54篇 |
1972年 | 55篇 |
1970年 | 74篇 |
1969年 | 53篇 |
1968年 | 64篇 |
1967年 | 62篇 |
排序方式: 共有10000条查询结果,搜索用时 734 毫秒
71.
M. Takagi K. Taniguchi T. Urasawa S. Urasawa T. Shirahata H. Goto 《Archives of virology》1994,139(1-2):209-215
Summary Antigenic and genomic properties of equine rotavirus strain CH3 isolated in Japan were studied by cross-neutralization tests and nucleotide sequence determination of the VP4 and VP7 genes. It was shown that the strain CH3 belongs to G14 and shares VP4 genotype with strain H2.The nucleotide sequence data reported in this paper appear in the DDBJ, EMBL and GeneBank nucleotide sequence detabases under the accession numbers D25228 (VP4 of strain CH3) and D25229 (VP7 of strain CH3). 相似文献
72.
Okamoto T Jeong SY Takahashi Y Baughman KW Ogata K Goto J Kanazawa I 《International journal of molecular medicine》2001,8(4):413-416
Calcium channel blocker is useful for a variety of purposes and is effective for preventing hepatitis elicited by different inducers, suggesting its possible clinical application for treating hepatitis. The alpha1-subunit of the dihydropyridine-sensitive L-type calcium channel is a target of calcium channel blocker. For clinical application of calcium channel blocker, it is important to analyze the expression of the L-type calcium channel in the liver. However, the subtype of the L-type calcium channel alpha1-subunit expressed in the liver was not known. In the present study, the alpha1-subunit of the calcium channel expressed in human liver was systematically analyzed. The alpha1D subunit of the dihydropyridine-sensitive L-type voltage gated calcium channel is expressed relatively strongly in the liver and may play an important role in the liver. 相似文献
73.
Contamination of transplantable human tumor-bearing lines by Helicobacter hepaticus and its elimination
下载免费PDF全文
![点击此处可从《Journal of clinical microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Goto K Ishihara KI Kuzuoka A Ohnishi Y Itoh T 《Journal of clinical microbiology》2001,39(10):3703-3704
Helicobacter hepaticus contaminating nonfrozen tumors was transmissible to severe combined immunodeficient (SCID) mice, but the organism in cryopreserved samples was not. This suggests that H. hepaticus has the ability to spread via biomaterials and that freezing-thawing is able to reduce the numbers of organisms to levels insufficient for subcutaneous infection of SCID mice. 相似文献
74.
Histological and histochemical analyses were performed on the anterior latissimus dorsi muscle (ALD, red muscle) and the posterior latissimus dorsi muscle (PLD, white muscle) in normal (line 412) and dystrophic chickens (line 413) from 19 day embryos to 6 weeks of age. PLD, the white muscle, in dystrophic chickens showed higher percentages of red and intermediate fibres than those of normal chickens during the early development of muscles. Increases of the oxidative enzyme activities and the numbers of NADH--TR formazan granules in the white fibres of PLD were already found at 1 week of age in dystrophic chicken. Fibre types, oxidative enzyme activities and NADH--TR formazan granules showed no differences in ALD between normal and dystrophic chickens. These results suggest that increases of oxidative enzyme activities and formazan granule numbers and incomplete fibre type differentiation in PLD of dystrophic chickens are early pathological processes in such birds. 相似文献
75.
Local secretory immunoglobulin A and postimmunization gastritis correlate with protection against Helicobacter pylori infection after oral vaccination of mice 总被引:13,自引:0,他引:13
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
C57BL/6 mice were orally immunized with five weekly doses of 2 mg, 200 microgram, or 2 microgram of Helicobacter pylori (Sydney strain) whole-cell sonicate combined with cholera toxin. One week after the last vaccination, mice were challenged with 5 x 10(7) CFU of live H. pylori three times at 2-day intervals. At 6 or 18 weeks after the challenge, mice were sacrificed and bacterial cultures and histological studies of the stomach were performed. Vaccination with 2 mg/session or 200 microgram/session inhibited H. pylori colonization by 90 and 100%, respectively. These mice were considered protected. Lower levels of H. pylori-specific immunoglobulin A (IgA) were detected in fecal and saliva samples before challenge. However, a significant increase in IgA secretion in mucosal tissue and a higher labeling index for IgA-positive lumina of pyloric glands were noted in these mice in response to challenge and in a vaccine dose-dependent manner. In protected mice, however, severe gastritis characterized by marked infiltration of inflammation mononuclear cells was noted at 6 weeks after challenge, compared with the gastritis seen in unprotected mice or nonvaccinated, ordinarily infected mice. Marked expression of gamma interferon mRNA was detected in the stomach of all protected mice, and 50% of these mice expressed interleukin 4 (IL-4) or IL-5 mRNA. Our findings suggest that local secretory IgA antibody and severe postimmunization gastritis correlate well with protection of mice against H. pylori infection. 相似文献
76.
77.
Gondo S Yanase T Okabe T Tanaka T Morinaga H Nomura M Goto K Nawata H 《Genes to cells : devoted to molecular & cellular mechanisms》2004,9(12):1239-1247
Bone marrow stem cells develop into haematopoietic and mesenchymal lineages, but have not been known to participate in steroidogenic cell production. Steroidogenic factor 1 (SF-1), also designated adrenal 4 binding protein (Ad4BP), is an essential orphan nuclear receptor for steroidogenesis as well as for adrenal and gonadal gland development. In the present study, we revealed that the adenovirus-mediated forced expression of SF-1 can transform cultured primary long-term cultured bone marrow cells into steroidogenic cells, showing the de novo synthesis of multiple steroid hormones in response to adrenocorticotropic hormone (ACTH). This finding may provide an initial step in innovative autograft cell transfer therapy for steroid hormone deficiencies. 相似文献
78.
79.
Growth of Bacteroides fragilis in Rabbit Tracheal Organ Culture: Anaerobiosis and Tissue Respiration
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Tsukasa Murakami Tohey Matsuyama Setsuko Shiraishi Bunji Hagihara 《Infection and immunity》1981,34(3):1062-1064
Rabbit tracheal explants supporting growth of inoculated Bacteroides fragilis in air were shown to keep low oxygen tension. Treating the explants with sodium azide induced high oxygen tension and arrested reversibly the growth of B. fragilis. 相似文献
80.
Suzuki T Muraki Y Yasukochi T Zhang H Kori Y Wakamatsu E Hayashi T Goto D Ito S Tsutsumi A Sumichika H Sumida T Matsumoto I 《Autoimmunity reviews》2005,4(7):475-478
Anti-glucose-6-phosphate isomerase (GPI) antibodies (Abs) solely induce arthritis in mice. High titers of anti-GPI Abs are found in some patients with rheumatoid arthritis (RA), but their pathogenic role remains elusive. The aim of this study was to evaluate the pathogenic role of anti-GPI Abs in cynomolgus monkeys. IgG fractions were separated from sera of anti-GPI Abs-positive RA patients and healthy subjects and directly injected into the metacarpophalangeal joints of 4 cynomolgus monkeys. At day 16, the joints were harvested and examined histologically and immunohistochemically. The expression of C5a receptor (C5aR) molecule in the synovium was quantified by real-time PCR using cDNA from monkey joints. In monkey joints, IgG including anti-GPI Abs resulted in recruitment of granulocytes and mononuclear cells, strong deposition of human IgG on the articular surface, and overexpression of C5aR, but no joint swelling. No infiltrated cells or IgG deposition were observed in monkeys injected with IgGs from healthy subjects. Our results suggest that IgG fraction from RA patients including anti-GPI Abs may play a crucial role in the generation of synovitis in monkeys, although the pathogenesis of anti-GPI Abs in RA patients is still uncertain. 相似文献