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991.
M Kohara S Naito K Arakawa J Miyata J Chihara T Taguchi S Takebayashi 《Journal of clinical & laboratory immunology》1985,18(4):157-160
41 Japanese patients with primary IgA nephropathy (IgA GN) were determined for HLA-A, -B, -C and -DR antigens. The frequency of HLA-DR4 increased slightly in total patients compared with 63 normal control persons, but this increase was not statistically significant. However, 24 patients with spherical mesangial dense deposits (SMDD) in electron micrograph out of 41 patients with IgA GN were found to have markedly increased incidence of HLA-DR4 (chi 2 = 11.52, Relative risk = 6.45, Corrected p less than 0.03). In contrast to this finding, 17 patients without SMDD had no association with any particular HLA antigens. These results suggest that IgA GN might be subdivided by the association of HLA and electron microscopic findings into at least 2 types. 相似文献
992.
S Ogahara I Michinaga T Hiratsuka K Abe S Naito 《Nihon rinsho. Japanese journal of clinical medicine》1992,50(12):3072-3078
The association between idiopathic nephropathy and HLA-class II antigen has been reported in many ethnic groups. We attempted to ascertain the HLA regions more specifically, associated with Japanese idiopathic membranous nephropathy (IMN), IgA nephropathy (IgAN) and minimal change nephrotic syndrome (MCNS), by examining HLA-class II genes. DNA typing of HLA-class II genes showed that IMN was associated with HLA-DRB1*1501-DRB5*0101-DQA1*0102-DQB1*0602, IgA with HLA-DQA1*0301, and MCNS with DQB1*0302. We also found a common epitope of HLA-class II (at 38th amino acid position of HLA-DR beta in IMN, at 55th of HLA-DQ beta in MCNS) in Japanese and Caucasian patients. These particular epitopes seem to be important for the susceptibility to IMN or MCNS. 相似文献
993.
Miyauchi S Gopal E Thakkar SV Ichikawa S Prasad PD Ganapathy V 《The Journal of pharmacology and experimental therapeutics》2007,321(1):257-264
We recently identified a novel opioid peptide transport system in the retinal pigment epithelium that transports opioid peptides by a Na+/Cl--dependent process. Here we describe a similar transport system expressed in SK-N-SH cells (a human neuronal cell line) and show for the first time that the activity of the transport system is modulated differentially by lysine and small nonopioid peptides. The transport process in SK-N-SH cells, monitored with deltorphin II as the substrate, is Na+/Cl--dependent and interacts with several opioid peptides, consisting of 5 to 13 amino acids. The activity of this transport system is markedly stimulated by specific dipeptides and tripeptides, with significant stimulation observable at low micromolar concentrations. The ion dependence, Na+/Cl--activation kinetics, and opioid peptide selectivity of the transport system, however, remain unchanged. The stimulation by the modulatory peptides is associated with an increase in maximal velocity with no change in substrate affinity of the system. Amino acids have no or little effect on the transport system, with the exception of lysine. This cationic amino acid inhibits the transport system, with significant inhibition occurring at physiologic concentrations of the amino acid. The inhibitory effect is primarily associated with a decrease in the maximal velocity of the transport system with little change in substrate affinity. Methyl and ethyl esters of lysine retain the inhibitory potency, but most other structural analogs have no effect. The differential modulation of the transport system by lysine and specific small peptides has important implications in the biology and pharmacology of opioid peptides. 相似文献
994.
J. Augé J. Vent I. Agache L. Airaksinen P. Campo Mozo A. Chaker C. Cingi S. Durham W. Fokkens P. Gevaert A. Giotakis P. Hellings M. Herknerova V. Hox L. Klimek C. La Melia J. Mullol N. B. Muluk A. Muraro K. Naito O. Pfaar H. Riechelmann C. Rondon M. Rudenko B. Samolinski I. Tasca P. Tomazic K. Vogt M. Wagenmann G. Yeryomenko L. Zhang R. Mösges 《Allergy》2018,73(8):1597-1608
Nasal allergen challenge (NAC) is an important tool to diagnose allergic rhinitis. In daily clinical routine, experimentally, or when measuring therapeutic success clinically, nasal allergen challenge is fundamental. It is further one of the key diagnostic tools when initiating specific allergen immunotherapy. So far, national recommendations offered guidance on its execution; however, international divergence left many questions unanswered. These differences in the literature caused EAACI to initiate a task force to answer unmet needs and find a consensus in executing nasal allergen challenge. On the basis of a systematic review containing nasal allergen challenges of the past years, task force members reviewed evidence, discussed open issues, and studied variations of several subjective and objective assessment parameters to propose a standardized way of a nasal allergen challenge procedure in clinical practice. Besides an update on indications, contraindications, and preparations for the test procedure, main recommendations are a bilaterally challenge with standardized allergens, with a spray device offering 0.1 mL per nostril. A systematic catalogue for positivity criteria is given for the variety of established subjective and objective assessment methods as well as a schedule for the challenge procedure. The task force recommends a unified protocol for NAC for daily clinical practice, aiming at eliminating the previous difficulty of comparing NAC results due to unmet needs. 相似文献
995.
Estimated number of off‐target candidate sites for antisense oligonucleotides in human mRNA sequences
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Tokuyuki Yoshida Yuki Naito Kiyomi Sasaki Eriko Uchida Yoji Sato Mikihiko Naito Toru Kawanishi Satoshi Obika Takao Inoue 《Genes to cells : devoted to molecular & cellular mechanisms》2018,23(6):448-455
Antisense oligonucleotide (ASO) therapeutics are single‐stranded oligonucleotides which bind to RNA through sequence‐specific Watson–Crick base pairings. A unique mechanism of toxicity for ASOs is hybridization‐dependent off‐target effects that can potentially occur due to the binding of ASOs to complementary regions of unintended RNAs. To reduce the off‐target effects of ASOs, it would be useful to know the approximate number of complementary regions of ASOs, or off‐target candidate sites of ASOs, of a given oligonucleotide length and complementarity with their target RNAs. However, the theoretical number of complementary regions with mismatches has not been reported to date. In this study, we estimated the general number of complementary regions of ASOs with mismatches in human mRNA sequences by mathematical calculation and in silico analysis using several thousand hypothetical ASOs. By comparing the theoretical number of complementary regions estimated by mathematical calculation to the actual number obtained by in silico analysis, we found that the number of complementary regions of ASOs could be broadly estimated by the theoretical number calculated mathematically. Our analysis showed that the number of complementary regions increases dramatically as the number of tolerated mismatches increases, highlighting the need for expression analysis of such genes to assess the safety of ASOs. 相似文献
996.
997.
998.
Characteristic microglial features in patients with hereditary diffuse leukoencephalopathy with spheroids
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![点击此处可从《Annals of neurology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Mari Tada MD PhD Takuya Konno MD PhD Masayoshi Tada MD PhD Toshiyuki Tezuka MD PhD Takeshi Miura MD Naomi Mezaki MD Ken‐ichi Okazaki MD PhD Musashi Arakawa MD PhD Kyoko Itoh MD PhD Toru Yamamoto MD PhD Hideaki Yokoo MD PhD Nobuaki Yoshikura MD Kenji Ishihara MD Masao Horie PhD Hirohide Takebayashi MD PhD Yasuko Toyoshima MD PhD Makoto Naito MD PhD Osamu Onodera MD PhD Masatoyo Nishizawa MD PhD Hitoshi Takahashi MD PhD Takeshi Ikeuchi MD PhD Akiyoshi Kakita MD PhD 《Annals of neurology》2016,80(4):554-565
999.
Mori M Ohtani H Naito Y Sagawa M Sato M Fujimura S Nagura H 《The Tohoku journal of experimental medicine》2000,191(2):113-118
CD8+ T cells infiltrating within cancer cell nests in human colorectal cancer were associated with a favorable patients' survival, suggesting the presence of anti-tumor immunity. The present study was designed to examine this concept in non-small cell lung cancer (NSCLC) by a retrospective analysis of 128 surgically resected cases. Immunohistochemical analysis showed that the number of CD8+ T cells within cancer cell nests in NSCLC was related to the histological subtype (large cell carcinoma or squamous cell carcinoma > adenocarcinoma) and the degree of dedifferentiation (undifferentiated type > differentiated type). In contrast to colorectal cancer, the number of CD8+ T cells in NSCLC had no statistically significant impact on the patients' survival. The present study demonstrated that the degree of infiltration of CD8+ T cells within cancer cell nests is dependent on the dedifferentiation of cancer cells in NSCLC, which could be one of the important aspects for the study of tumor immunity. 相似文献
1000.
Vein wrapping facilitates basic fibroblast growth factor‐induced heme oxygenase‐1 expression following chronic nerve constriction injury
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![点击此处可从《Journal of orthopaedic research》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Naoya Hirosawa Kentaro Uchida Kazuki Kuniyoshi Kenichi Murakami Gen Inoue Masayuki Miyagi Yusuke Matsuura Sumihisa Orita Kazuhide Inage Takane Suzuki Masashi Takaso Seiji Ohtori 《Journal of orthopaedic research》2018,36(3):898-905