全文获取类型
收费全文 | 38034篇 |
免费 | 1935篇 |
国内免费 | 271篇 |
专业分类
耳鼻咽喉 | 454篇 |
儿科学 | 631篇 |
妇产科学 | 604篇 |
基础医学 | 4601篇 |
口腔科学 | 822篇 |
临床医学 | 2588篇 |
内科学 | 11148篇 |
皮肤病学 | 615篇 |
神经病学 | 2655篇 |
特种医学 | 1223篇 |
外科学 | 6515篇 |
综合类 | 159篇 |
一般理论 | 4篇 |
预防医学 | 995篇 |
眼科学 | 761篇 |
药学 | 2363篇 |
中国医学 | 75篇 |
肿瘤学 | 4027篇 |
出版年
2023年 | 247篇 |
2022年 | 464篇 |
2021年 | 867篇 |
2020年 | 499篇 |
2019年 | 632篇 |
2018年 | 773篇 |
2017年 | 608篇 |
2016年 | 736篇 |
2015年 | 766篇 |
2014年 | 1065篇 |
2013年 | 1281篇 |
2012年 | 2048篇 |
2011年 | 2270篇 |
2010年 | 1276篇 |
2009年 | 1037篇 |
2008年 | 1920篇 |
2007年 | 2119篇 |
2006年 | 2013篇 |
2005年 | 1944篇 |
2004年 | 1858篇 |
2003年 | 1757篇 |
2002年 | 1837篇 |
2001年 | 1029篇 |
2000年 | 1041篇 |
1999年 | 921篇 |
1998年 | 453篇 |
1997年 | 371篇 |
1996年 | 337篇 |
1995年 | 281篇 |
1994年 | 253篇 |
1993年 | 225篇 |
1992年 | 654篇 |
1991年 | 670篇 |
1990年 | 579篇 |
1989年 | 585篇 |
1988年 | 544篇 |
1987年 | 521篇 |
1986年 | 443篇 |
1985年 | 444篇 |
1984年 | 336篇 |
1983年 | 250篇 |
1979年 | 238篇 |
1978年 | 135篇 |
1977年 | 145篇 |
1974年 | 133篇 |
1973年 | 120篇 |
1972年 | 140篇 |
1971年 | 131篇 |
1969年 | 140篇 |
1967年 | 128篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
To elucidate the molecular basis for endocrine tumorigenesis, p53 mutations in human endocrine tumors were analyzed by using polymerase chain reaction-single strand conformation polymorphism. Exons 5 through 10 of the p53 gene were studied in genomic DNAs from 134 primary endocrine tumors and 6 human endocrine cancer-derived cell lines. Mutations were detected and identified in 4 endocrine tumors, including one parathyroid adenoma and three thyroid carcinoma cell lines. The sites of these mutations were in exons 5 (codon 151 and 152) and 7 (codon 248 and 255). In all of three tumor cell lines, but not in a parathyroid adenoma, the normal allele encoding the p53 gene was lost. However, p53 mutations were not found in any other endocrine tumors or cell lines. Based upon these results, we concluded that the p53 gene may play a role in the tumorigenesis of a limited number of parathyroid adenoma and thyroid cancers, and that the p53 mutation with an allelic loss of the p53 gene is an important factor in malignant tumorigenesis of the thyroid gland. 相似文献
92.
H Sasano Y Saito H Nagura R Kudo M Rojas S G Silverberg 《International journal of gynecological pathology》1991,10(3):252-259
The lectin-binding properties of 44 cases of serous and mucinous ovarian cystadenoma, tumor of low malignant potential (LMP), and invasive carcinoma were examined histochemically. Wheat germ agglutinin (WGA), concanavalin A (con A), Ulex europaeus agglutinin I (UEA-I), peanut agglutinin (PNA), Ricinus communis agglutinin I (RCA-I), soybean agglutinin (SBA), and Robina pseudoaccacia (RPA) were employed. All the lectins examined were bound to neoplastic epithelial cells of benign and malignant tumors, but none bound exclusively to ovarian tumor cells. Different lectin-binding patterns between serous and mucinous neoplasms were observed, with the exception of RPA. UEA-I, con A, RPA, and PNA in serous neoplasms and UEA-I, RPA, and WGA in mucinous neoplasms demonstrated lectin-binding properties of LMP tumors intermediate between those of cystadenoma and invasive carcinoma. These findings indicate that serous and mucinous ovarian neoplasms contain different glycoconjugates, that malignant transformation of the neoplasms is associated with alteration of these glycoconjugates, and especially that LMP tumors have a different composition of cellular glycoconjugates from that of invasive ovarian carcinoma. 相似文献
93.
S Yamashita K Taniguchi H Morimitsu H Suzu H Kanetake Y Saito Y Yushita T Sakuragi 《Hinyokika kiyo. Acta urologica Japonica》1992,38(5):519-524
Between 1986 and 1989, 14 patients undergoing cystectomy for bladder cancer, in pathological stage high risk pT2 group, pT3-4 and/or with N+ disease, received postoperative adjuvant methotrexate, vinblastine, doxorubicin and cisplatin (M-VAC) chemotherapy. Of the 14 patients 10 were alive with no evidence of disease for an average of 41 months. Tumor recurrence was seen in 4 patients (bone in 2, lungs in 1, brain in 1 patient). Of the 4 patients, 3 patients died of cancer progression at an average of 26 months and 1 patient was alive with tumor for 30 months. Their actual survival rate at 64 months was 70%, which was significantly higher than that of the historical control groups (1974-1981: 18%, 1982-1985: 46%). Although postoperative adjuvant M-VAC chemotherapy for invasive bladder cancer seemed effective in this study, a controlled randomized study will be necessary to conclude if it could be of real benefit for these patients. 相似文献
94.
Toshihiro Kawamura Hiroshi Motoyama Atsushi Yanaihara Takeshi Yorimitsu Akane Arichi Yasuhiro Karasawa Kahori Suga Kaoru Miya Seika Ishikawa Shiho Mizushima Makiko Kawamura 《Reproductive Medicine and Biology》2007,6(1):53-57
Aim: To compare the clinical outcomes of cryopreserved-thawed embryo transfer among patients with a normal menstrual cycle who had natural or hormone-replacement cycles.
Methods: From January 2004 to June 2006, cryopreserved embryos following conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) were thawed and transferred in a total of 720 natural cycles and 136 hormone-replacement cycles.
Results: Cryopreserved-thawed embryo transfer in patients who had a natural or hormone-replacement cycle resulted in clinical pregnancy in 43.1% and 40.4%, respectively; a rate of miscarriage of 14.5% and 23.6%, respectively; and a rate of ongoing pregnancy and delivery of 36.5% and 30.9%, respectively. None of these differences were statistically significant.
Conclusions: Patients with a normal menstrual cycle who have natural or hormone-replacement cycles can be expected to have comparable clinical outcomes with cryopreserved-thawed embryo transfer. (Reprod Med Biol 2007; 6 : 53–57) 相似文献
Methods: From January 2004 to June 2006, cryopreserved embryos following conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) were thawed and transferred in a total of 720 natural cycles and 136 hormone-replacement cycles.
Results: Cryopreserved-thawed embryo transfer in patients who had a natural or hormone-replacement cycle resulted in clinical pregnancy in 43.1% and 40.4%, respectively; a rate of miscarriage of 14.5% and 23.6%, respectively; and a rate of ongoing pregnancy and delivery of 36.5% and 30.9%, respectively. None of these differences were statistically significant.
Conclusions: Patients with a normal menstrual cycle who have natural or hormone-replacement cycles can be expected to have comparable clinical outcomes with cryopreserved-thawed embryo transfer. (Reprod Med Biol 2007; 6 : 53–57) 相似文献
95.
Comparative in vitro and in vivo activity of rifabutin and rifampicin against Mycobacterium avium complex 总被引:9,自引:0,他引:9
In vitro antimicrobial activity of rifabutin and rifampicin against various mycobacteria, including the Mycobacterium avium complex, was evaluated by the agar dilution method, using 7H10 agar medium. The activity of rifabutin based on MIC50 and MIC90 was higher than that of rifampicin, against all the acid-fast organisms tested. Microbicidal activity of rifabutin against the M. avium complex phagocytosed in mouse peritoneal or alveolar macrophages was greater than that of rifampicin. Both rifabutin and rifampicin had therapeutic effects against murine infections induced by M. avium complex. Rifabutin was somewhat more effective than rifampicin in mice. 相似文献
96.
97.
98.
Minimal osteonecrosis (MON) is a small and eccentrically localized lesion of osteonecrosis (ON) of the femoral head. This form of ON shows clinically benign features, but may be indicative of the pathomechanism of ON. Of 180 patients (275 hips) with ON, 15 patients (19 hips) were found to have MON. Based on the geography of the femoral head, the lesions were grouped into four types: anterior (seven hips), superior (six hips), medial (five hips), and lateral (one hip). When the location of the lesion was compared with the normal vascular anatomy, a close correlation between the location and the segmental blood supply in the femoral head became evident. Histologic findings showed localized infarction of the trabeculae and bone marrow, but no evidence suggesting repeated episodes of infarction, which is an essential feature of typical ON. From these findings, MON may be a segmental infarct within the femoral head. Interruption of the blood flow may possibly occur not in the main trunk but in the terminal branch of the lateral epiphyseal or superior metaphyseal artery; such as ischemic event is probably a single brief attack, leading to this form of infarction in contrast to typical ON developing with prolonged or recurrent obstructions. 相似文献
99.
100.
Hiromi Oono Masae Nakagawa Atsushi Miyamoto Shigeru Ishiguro Akira Nishio 《Magnesium research》2002,15(3-4):153-160
We have previously shown that interleukin (IL)-1beta and tumour necrosis factor (TNF)-alpha mRNA levels in rat alveolar macrophages are increased in by endotoxin (lipopolysaccharide; LPS)- stimulation and further enhanced by culturing with low-Mg2+ medium. We have now investigated the mechanisms of underlying this enhancement by using some specific signal transduction inhibitors. The enhanced elevation of both mRNAs levels was suppressed by pretreatment with TMB-8 (which inhibits calcium release from the endoplasmic reticulum) or dexamethasone (which inhibits nuclear factor [NF]-kappaB and activator protein [AP]-1), but not with verapamil or nifedipine (which inhibits calcium channels). The enhancment of IL-1beta, but not TNF-alpha mRNA levels, was suppressed by pretreatment with W-7 (which inhibits calmodulin), whereas the enhancement of TNF-alpha mRNA levels was suppressed by pretreatment with U73122 (which inhibits phospholipase C). Curcumin (an inhibitor of AP-1), suppressed the increases in both mRNAs induced by low-Mg2+ medium alone, but had no suppressive effect on the levels of either mRNA after LPS-stimulation in low-Mg2+ medium. Pyrrolidine dithiocarbamate (an inhibitor of NF-kappaB) prevented the elevation of TNF-alpha mRNA levels induced by low-Mg2+ medium without LPS-stimulation, but had no suppressive effect on IL-1beta mRNA levels. From these results, we conclude that the enhanced elevation of IL-1beta and TNF-alpha mRNA levels seen in LPS-stimulated alveolar macrophages in low-Mg2+ medium occurs partly via the same, and partly via different, signaling pathways. 相似文献