全文获取类型
收费全文 | 2886篇 |
免费 | 190篇 |
国内免费 | 16篇 |
专业分类
耳鼻咽喉 | 10篇 |
儿科学 | 113篇 |
妇产科学 | 42篇 |
基础医学 | 530篇 |
口腔科学 | 77篇 |
临床医学 | 202篇 |
内科学 | 673篇 |
皮肤病学 | 95篇 |
神经病学 | 193篇 |
特种医学 | 66篇 |
外科学 | 295篇 |
综合类 | 9篇 |
预防医学 | 198篇 |
眼科学 | 68篇 |
药学 | 226篇 |
中国医学 | 14篇 |
肿瘤学 | 281篇 |
出版年
2023年 | 26篇 |
2022年 | 38篇 |
2021年 | 74篇 |
2020年 | 46篇 |
2019年 | 64篇 |
2018年 | 65篇 |
2017年 | 71篇 |
2016年 | 74篇 |
2015年 | 79篇 |
2014年 | 80篇 |
2013年 | 120篇 |
2012年 | 184篇 |
2011年 | 200篇 |
2010年 | 115篇 |
2009年 | 125篇 |
2008年 | 187篇 |
2007年 | 184篇 |
2006年 | 217篇 |
2005年 | 196篇 |
2004年 | 189篇 |
2003年 | 146篇 |
2002年 | 152篇 |
2001年 | 23篇 |
2000年 | 16篇 |
1999年 | 35篇 |
1998年 | 36篇 |
1997年 | 23篇 |
1996年 | 18篇 |
1995年 | 22篇 |
1994年 | 24篇 |
1993年 | 24篇 |
1992年 | 21篇 |
1991年 | 23篇 |
1990年 | 21篇 |
1989年 | 16篇 |
1988年 | 11篇 |
1987年 | 21篇 |
1986年 | 15篇 |
1985年 | 19篇 |
1984年 | 11篇 |
1983年 | 9篇 |
1982年 | 9篇 |
1981年 | 7篇 |
1980年 | 9篇 |
1978年 | 8篇 |
1977年 | 4篇 |
1976年 | 3篇 |
1974年 | 8篇 |
1973年 | 4篇 |
1971年 | 3篇 |
排序方式: 共有3092条查询结果,搜索用时 984 毫秒
51.
Akira Kasuya Jun‐ichi Sakabe Reiko Kageyama Shigeki Ikeya Toshiharu Fujiyama Yoshiki Tokura 《The Journal of dermatology》2014,41(6):542-544
The polymerase chain reaction (PCR) assay for varicella zoster virus (VZV), herpes simplex virus (HSV)‐1 and HSV‐2 is available for use. Sometimes the differential diagnosis of the generalized herpes zoster (HZ), HSV1/2, and drug eruption is difficult. We report a case of HZ followed by the vesicular erythema multiforme (EM)‐like lesion. In this case the use of PCR was of great assistance. A 78‐year‐old Japanese man without any significant previous history of disease was admitted to our hospital complaining of zosteriform vesicle on an erythematous base from his right shoulder to the upper arm. We diagnosed him with HZ at the level of right Th2. In spite of the prompt start of antiviral therapy, a secondary new vesiculous erythema developed on his trunk. Clinically, it was quite difficult to differentiate the lesion from the generalized HZ. Rapid PCR assay of effusion and crust for VZV was performed. A PCR assay of VZV was positive for the crust taken from the primary lesion, while it was negative for the effusion and crust of the secondary widespread lesion. We diagnosed the secondary widespread lesion as an EM‐type drug eruption induced by acyclovir, or an EM associated with herpes zoster. We then stopped the use of acyclovir and applied steroid ointment of a very strong class for the secondary lesions, which improved after a few days. A PCR assay for VZV was useful for ruling out the generalized HZ in our case with secondary developed vesiculous lesions. 相似文献
52.
53.
Therapeutic efficacy of intense pulsed light in patients with refractory meibomian gland dysfunction
Purpose
To evaluate the efficacy and safety of intense pulsed light (IPL) combined with meibomian gland expression (MGX) for treatment of refractory meibomian gland dysfunction (MGD).Methods
Ninety eyes of 45 patients were randomly assigned to receive either the combination of IPL and MGX or MGX alone (control). Each eye underwent eight treatment sessions at 3-week intervals. Parameters were evaluated before and during treatment as well as at 3–11 weeks after the last treatment session. Measured parameters included the Standard Patient Evaluation of Eye Dryness (SPEED) questionnaire score, noninvasive breakup time (NIBUT), fluorescein breakup time (BUT), lipid layer grade, lipid layer thickness (LLT), lid margin abnormalities, corneal and conjunctival fluorescein staining (CFS) score, meibum grade, and meiboscore.Results
A significant improvement in lipid layer grade was apparent in the IPL-MGX group from 6 to 32 weeks after treatment onset (adjusted P?<?0.001) but was not observed in the control group. The IPL-MGX group also showed significant improvements in LLT, NIBUT, BUT, lid margin abnormalities, and meibum grade compared with the control group at 24 and 32 weeks (adjusted P?<?0.001) as well as significant improvements in the SPEED score at 32 weeks (adjusted P?=?0.044) and in CFS score at 24 (adjusted P?=?0.015) and 32 (adjusted P?=?0.006) weeks.Conclusions
The combination of IPL and MGX improved homeostasis of the tear film and ameliorated ocular symptoms in patients with refractory MGD and is thus a promising modality for treatment of this condition. 相似文献54.
55.
Nakamura S Nakamura R Shibata K Kobayashi M Sahara N Shigeno K Shinjo K Naito K Ohnishi K Kasahara N Iwaki Y 《European journal of haematology》2004,73(4):285-294
Adeno-associated virus (AAV) vector system has several useful advantages with regard to in vitro and in vivo gene transfer. However, their usages have been limited by cumbersome and labor-intensive vector production in the traditional method. To overcome limitations in AAV production, in this report, we explored the possibility of generating AAV packaging cell line, 293T R/C.VA.E2A.E4. cells, by using lentivirus-mediated transduction of Rep/Cap gene of AAV-2, VA RNA, E2A, and E4 genes of Ad5 into 293T cells. In packaging cell lines, it is important that supply of the AAV vector can be stably performed for long time. We showed that the 293T R/C.VA.E2A.E4. cells have stably maintained the transduced components after more than 10 passages and yielded high-titer AAV vectors, and the titer of AAV vectors did not decline even if culture of the packaging cells was continued for long time. The Rep/Cap and E4 gene products caused no remarkable cytotoxicity. The 293T R/C.VA.E2A.E4. cells might be able to tolerate the Rep/Cap and E4 gene products, or have less copy numbers of the Rep/Cap and E4 genes than the traditional method. Moreover, we showed that the AAV vectors derived from 293T R/C.VA.E2A.E4. cells infected the primary human CD34+ haematopoietic progenitor cells with high efficiency (50-70%). In the 293T R/C.VA.E2A.E4. cells, the AAV vectors can be generated by the transfection of one AAV vector plasmid, and large-scale AAV production can be easily achieved. It is important that cumbersome, variable, and costly transfection is avoided. 相似文献
56.
Pharmacobezoar complicating treatment with sodium alginate 总被引:1,自引:0,他引:1
Kaneko H Tomomasa T Kubota Y Todokoro M Kato M Miyazawa R Suzuki T Hatori Y Kunimoto F Yamamoto K Morikawa A 《Journal of gastroenterology》2004,39(1):69-71
We encountered a gastric bezoar that had developed in a 9-year-old girl treated with sodium alginate (Alloid G) for acute gastritis associated with systemic lupus erythematosus. A hard mass palpated in the left upper abdomen proved, upon gastric endoscopy, to be an intragastric foreign body. Sodium alginate was detected in an analysis of a sample from this bezoar. In an in vitro simulation, sodium alginate solidified when mixed with the patients other medicines. The bezoar caused no complications, and disappeared spontaneously after discontinuation of the medications. This case indicates that this sodium alginate preparation, Alloid G, can be a cause of pharmacobezoar. 相似文献
57.
The in vitro priming of tumor-specific T cells by dendritic cells (DCs) phagocytosing killed tumor cells can be augmented in the presence of antitumor monoclonal antibody (mAb). We investigated whether DCs phagocytosing killed lymphoma cells coated with tumor-specific antibody could elicit antitumor immunity in vivo. Irradiated murine 38C13 lymphoma cells were cocultured with bone marrow-derived DCs in the presence or absence of tumor-specific mAb. Mice vaccinated with DCs cocultured with mAb-coated tumor cells were protected from tumor challenge (60% long-term survival), whereas DCs loaded with tumor cells alone were much less effective. The opsonized whole tumor cell-DC vaccine elicited significantly better tumor protection than a traditional lymphoma idiotype (Id) protein vaccine, and in combination with chemotherapy could eradicate preexisting tumor. Moreover, the DC vaccine protected animals from both wild-type and Id-negative variant tumor cells, indicating that Id is not a major target of the induced tumor immunity. Protection was critically dependent upon CD8(+) T cells, with lesser contribution by CD4(+) T cells. Importantly, opsonized whole tumor cell-DC vaccination did not result in tissue-specific autoimmunity. Since opsonized whole tumor cell-DC and Id vaccines appear to target distinct tumor antigens, optimal antilymphoma immunity might be achieved by combining these approaches. 相似文献
58.
59.
60.
Daisuke Kozai Reiko Sakaguchi Tomohiko Ohwada Yasuo Mori 《Current Neuropharmacology》2015,13(2):266-278
The transient receptor potential (TRP) proteins are a family of ion channels that act as
cellular sensors. Several members of the TRP family are sensitive to oxidative stress mediators.
Among them, TRPA1 is remarkably susceptible to various oxidants, and is known to mediate
neuropathic pain and respiratory, vascular and gastrointestinal functions, making TRPA1 an
attractive therapeutic target. Recent studies have revealed a number of modulators (both activators and inhibitors) that act
on TRPA1. Endogenous mediators of oxidative stress and exogenous electrophiles activate TRPA1 through oxidative
modification of cysteine residues. Non-electrophilic compounds also activate TRPA1. Certain non-electrophilic
modulators may act on critical non-cysteine sites in TRPA1. However, a method to achieve selective modulation of
TRPA1 by small molecules has not yet been established. More recently, we found that a novel N-nitrosamine compound
activates TRPA1 by S-nitrosylation (the addition of a nitric oxide (NO) group to cysteine thiol), and does so with
significant selectivity over other NO-sensitive TRP channels. It is proposed that this subtype selectivity is conferred
through synergistic effects of electrophilic cysteine transnitrosylation and molecular recognition of the non-electrophilic
moiety on the N-nitrosamine. In this review, we describe the molecular pharmacology of these TRPA1 modulators and
discuss their modulatory mechanisms. 相似文献