Preliminary note on biometric data relating to the human coraco-acromial arch

A biometric study based on 20 human scapulae made it possible to specify the variations in the gap of the coraco-acromial arch in relation to its depth and height. A graphic representation in rectangular coordinates, then in spatial representation in relation to the three planes of reference, leads to the following findings: the bony variations in the arch occur essentially: at the coracoid apophysis, and two types of arch can be distinguished depending on the predominance of bony or of ligamentous components.     

牛黄解毒片的三维高效液相色谱法鉴定和指标成分的定量

用三维高效液相色谱(three dimensional HPLC)法对中成药牛黄解毒片进行了分析。结果表明该法用于牛黄解毒片中单味中药鉴定及指标成分(marker substance)含量测定均得到较满意结果。     

Anti-idiotypic antibodies specific for a pathologic anti-Pr2 cold agglutinin

The heterogeneity of human red cell (RBC) autoantibodies may be assessed by using anti-idiotypic antibodies. In this study, mouse monoclonal anti-idiotypic antibodies were produced against a pathologic RBC autoantibody with anti-Pr2 specificity. Epstein-Barr virus-transformed B-cell clones were established from a patient who had splenic lymphoma and associated immune hemolysis due to an anti-Pr2 cold autoantibody. Two of the eight clones producing this autoantibody were used to immunize mice for the establishment of hybridomas, and four monoclonal anti-idiotypic antibodies were isolated (2 IgG1 kappa and 2 IgM kappa). By the use of these anti-idiotypic antibodies, strong crossreactivity was seen on enzyme-linked immunosorbent assay with other anti-Pr2-producing clones from the same patient, but no cross-reactivity was seen with RBC autoantibodies from other individuals having anti-Pr or different specificities. Each of the anti-idiotypic antibodies inhibited hemagglutination (HA) by the patient's anti-Pr2 but failed to inhibit HA by antisera of a different RBC specificity. Cross-competition experiments indicated that all of the anti-idiotypic antibodies may recognize the same or a closely related idiotope on the anti-Pr2 autoantibody. These studies suggested that the four anti-idiotypic antibodies are directed against the same (or closely related) idiotypic determinant(s), unique to this patient's anti-Pr2 and located at or near the antigen-binding site. These anti-idiotypic antibodies may be useful tools for the study of this autoimmune response or for the development of immune therapeutic agents.(ABSTRACT TRUNCATED AT 250 WORDS)     

Effect of human saliva and various compounds on the adsorption of the bacterium Streptococcus mutans to hydroxyapatite

Adsorption of Streptococcus mutans to hydroxyapatite (HA) was significantly reduced by pre-treating the HA with saliva and salivary components such as phosphoprotein and mucin. An even greater inhibition of adsorption was found when saliva was used as the incubation fluid. No significant difference in adsorption inhibition was observed for saliva obtained from caries-active as against caries-free subjects. Initial adherence of pronase and trypsin-treated Strep, mutans cells to HA disks was also significantly inhibited, whereas dextranase (free of protease activity) and hyaluronidase had no inhibitory effect relative to phosphatebuffered saline-treated control cells.     

Dietary effects on (pro)insulin biosynthesis and insulin-degrading activity in islets from sand rats

Sand rats were fed either a vegetable (vegetable group) or a standard pellet diet. After 14-16 weeks, the normoglycemic subgroup selected (pellet group) from the animals that had been maintained on the standard diet showed a modest increase in body weight. Plasma immunoreactive insulin levels were not significantly increased, but glucose-stimulated insulin release was elevated from islets isolated from sand rats of the pellet group. Insulin biosynthesis was estimated in vitro by measuring [3H]leucine incorporation into (pro)insulin at 1.5 or 15 mmol/l glucose. The rate of (pro)insulin biosynthesis was elevated only at 15 mmol/l glucose in islets from those normoglycemic sand rats fed the pellet diet when compared with islets from the vegetable group. Specific insulin-degrading activity, as determined by measuring degradation of 125I-labeled insulin, was also increased for islets from the pellet group. The metabolic state of these sand rats is thus associated with normoglycemia in vivo, and increased stimulated rates of insulin biosynthesis and degradation in pancreatic islets in vitro.     

In vitro studies of amniotic fluid and intrauterine growth retardation

Until now the intrauterine growth retardation remains as an insufficiently solved problem. The 3H-thymidine incorporation into the DNA of the isolated islets of Langerhans of neonatal Lewis rats was used as a marker for cell dividing activity in vitro. There was no inhibition effect on the 3H-thymidine incorporation in the presence of amniotic fluid from pregnancies with intrauterine growth retardation compared to those of normal pregnancies.