The tumorigenic phenotype of a mutated form of Fc gamma RIIB1, lacking the ability to generate soluble receptor and allowing a low-level of ligand binding

Non immunohematopoietic murine tumor cells ectopically expressing Fc gamma RIIB1 (B1) were recently shown to express a higher tumorigenicity phenotype than cells not expressing this receptor. Utilizing a genetic approach we studied the possible contribution of a soluble form of B1 to tumor enhancement. A mutated form of the B1, lacking the cleavage site responsible for the generation of soluble B1 was produced using gene splicing by overlap extension PCR. A deletion confirmed by sequence analysis from 172 to 178 residues was generated. Stable transfectants expressed the B1 deleted form (B1 Delta) both as specific RNA and as a membrane protein receptor allowing a low level of ligand binding. The soluble form of B1 was undetectable in tissue culture supernatants of Bib transfected cells while it was present in supernatants of wild type B1-transfectants. Stable B1 Delta transfectants were significantly more tumorigenic than negative control transfectants. Tumor incidence was almost as high as that of intact B1 and lagged in the latency period before the appearance of palpable tumors. It is suggested that the soluble B1 has a minimal contribution to tumor enhancement.     

与经皮腹膜后隙脾肾静脉分流术有关的形态学观测

目的为经皮腹膜后隙牌肾静脉分流术（PRESS）技术的开发提供形态学依据。方法选取30例经甲醛固定的成人尸体标本,观察牌静脉、左肾静脉的走行及两者之间的关系,并对相关数据进行测量。结果牌静脉紧靠肠系膜上动脉起端右前方与肠系膜上静脉汇合为门静脉,脾静脉与左肾静脉在肠系膜上动脉起点左侧位置关系较为恒定。结论经股静脉的插管须先进入左肾静脉,然后在肠系膜上动脉起端左侧穿向脾静脉。     

茯苓萜类的高效液相色谱指纹图谱研究

目的 建立茯苓三萜化合物的指纹图谱。方法 反相高效液相色谱法。结果 道地药材茯苓及不同地区收集的市场商品具有相当稳定的、相似程度很高的指纹图谱。结论 可利用液相色谱的特征峰控制茯苓及其制剂的质量。     

白鲜皮质量标准研究

目的：为中药白鲜皮制定2005年版药典标准。方法：采用TLC法鉴别、反相高效液相色谱法测定白鲜皮中活性成分末梣酮含量，并对各地产白鲜皮药材的水分、总灰分、酸不溶性灰分、浸出物含量进行测定。结论：本方法操作简单，重现性好，可用于白鲜皮药材的质量控制。     

多种血清标志物预测老年急性缺血性脑卒中患者卒中后认知障碍的研究

目的 分析多种血清标志物预测老年急性缺血性脑卒中（AIS）患者卒中后认知障碍（PSCI的价值。方法 回顾性收集2019年1月至2023年1月哈尔滨医科大学附属第一医院神经内科收治的326例老年AIS患者的资料。根据发病后6个月的简易精神状态量表（MMSE）评分,将患者分为PSCI组（MMSE评分≤26分）和非PSCI组（MMSE评分>26分）。比较两组患者的一般资料和血清指标。采用多因素Logistic回归分析老年AIS患者发生PSCI的危险因素,并采用受试者工作特征（ROC）曲线下面积（AUC）分析血清标志物预测老年AIS患者发生PSCI的效能。结果 326例患者中,127例（38.96%）在发病后6个月发生PSCI。PSCI组年龄>75岁、患糖尿病、有脑卒中史的患者比例,入院时NIHSS评分,同型半胱氨酸、尿酸水平,中性粒细胞/淋巴细胞比值（NLR）,低密度脂蛋白胆固醇（LDL-C）水平,C-反应蛋白（CRP）水平均高于非PSCI组,差异均有统计学意义（均P<0.05）;高密度脂蛋白胆固醇（HDL-C）低于非PSCI组,差异有统计学意义（P<0.05）。多因...     

芳香疗法用于妇科恶性肿瘤患者术后恶心呕吐的效果研究

目的 探讨芳香疗法缓解妇科恶性肿瘤术后患者恶心呕吐的效果.方法 选取2019年1月至2019年8月间中国医学科学院肿瘤医院收治的行手术治疗的202例妇科恶性肿瘤患者,按病房号奇偶数随机分为试验组(102例)和对照组(100例).对照组患者采用常规护理,试验组患者在常规护理基础上采用嗅柠檬香氛作为芳香疗法进行干预,比较两...     

A systematic review of the efficacy of donepezil hydrochloride combined with nimodipine on treating vascular dementia

Background:Vascular dementia (VaD) is a comprehensive syndrome related to the damage of cognitive function and various cerebral vascular illnesses. VaD is also generally recognized as the second most common type of dementia after Alzheimer disease, contributing to 30% of the dementia population in Asia and developing countries. The ability of donepezil hydrochloride and nimodipine had been respectively proven in improving cognitive function in vascular dementia. However, whether the combined application of both drugs contribute to better efficacy remains as a research hotspot. Studies had shown definite satisfactory result with such combination, however evidence-based evaluation of the efficacy is still lacking. Therefore, meta-analysis is employed in this study to evaluate the efficacy and safety of using donepezil hydrochloride combined with nimodipine in treating VaD to provide references for clinical treatments. The efficacy of donepezil hydrochloride combined with nimodipine on treating vascular dementia is systematically reviewed to provide evidence-based references for clinical applications.Methods:Both Chinese and English databases were searched from the start till August, 2020 for any RCT regarding the combined use of the 2 drugs in treating vascular dementia. Two investigators would later evaluate and screened out research and data based on an improved Jaded scale. Software Rev Man 5.3.0 was employed to carry out meta-analysis on clinical effificacy, mini-mental state examination (MMSE) ratings, activity of daily living (ADL) ratings, and clinical dementia scale (CDR) ratings.Results:Donepezil hydrochloride combined with nimodipine had demonstrated satisfactory efficacy on the treatment of vascular dementia. Improvements were namely spotted on MMSE scale, ADL scale, and CDR scale, with the utmost efficacy by 12 weeks after intervention.Conclusions:Donepezil hydrochloride combined with nimodipine had good efficacy in the treatment of patients with vascular dementia, mainly in terms of improving the Simple MMSE scores, the ability to use daily living scale (ADL) scores and the CDR, and the best results were obtained after 12 weeks of intervention. Such conclusion should be cautiously evaluated.     

老年人心力衰竭91例临床分析

目的探讨老年人心力衰竭的临床特点。方法回顾性分析91例老年人心力衰竭临床资料,与21例中青年人心力衰竭进行对比分析。结果(1)随着年龄的增加,NYHA心功能Ⅲ、Ⅳ的比例显著增加;(2)引起心力衰竭前3位病因分别为冠心病(67.9%)、高血压病(32.1%)、肺心病(26.8%),老年组及高龄组常为2-3种疾病并存;(3)各年龄组心力衰竭最常见的诱因是感染(59.8%),高龄组心律失常(51.4%)也是重要的诱因;(4)老年及高龄组症状、体征常不典型,易致漏诊误诊;(5)高龄组并发脑梗死(25.7%)、心律失常-房颤(34.3%)及死亡(17.4%)的比例增加。结论年龄越大,临床表现越不典型,病情越危重,并发症越多,预后不良。     

红芪有效成分及“入脾经”相关药理研究

对近年来红芪的成分检测结果及其现代药理学研究成果进行了详细的综述,指出了中药红芪的主要成分,同时发现红芪的药理学研究结论符合其中医理论中的＂入脾经＂功效,并对两者相关性进行总结分析,为红芪的研究与开发提供依据。