复方苦参颗粒对重度特应性皮炎51例的治疗效果

目的 评估复方苦参颗粒治疗重度特应性皮炎的疗效及安全性.方法 51例门诊病人参加此次研究.记录治疗前后的湿疹评分,以及治疗前后的嗜酸粒细胞(EOS)和血清总IgE值.复方苦参颗粒口服1～2g/次,3次/d,外用方1患部外洗1～2次,d,外用方2惠部外用2～5 R/d.结果 治疗后特应性皮炎患者症状明显改善,治疗前全部极重度,治疗后94.1%的患者改善为中度以下.瘙痒也明显改善,治疗前全部患者约76.9%有日常生活障碍,治疗后全部患者改善为日常生活没有障碍水平.皮疹和瘙痒的评分,与治疗前比较差异均有显著性(P<0.01).患者EOS和血清总IgE值治疗后都有显著下降,与治疗前比较差异有显著性(P<0.01).有效率94.1%.结论 复方苦参颗粒治疗重度特应性皮炎安全有效.     

非酒精性脂肪肝大鼠脂质代谢及病理变化的动态观察

目的 通过高脂饮食建立NAFLD大鼠模型,连续监测4～16周模型动物肝功能、脂质代谢、胰岛素抵抗及肝细胞凋亡在NAFLD进展过程中的变化情况及相瓦关系,为该模型在脂肪肝发病机制、脂肪肝治疗药物评价等方面的应用提供参考依据.方法 SD大鼠50只,除正常对照组外,其余动物饲喂高脂饲料,分别检测4,8,12,16周大鼠血清GLU、CHO、TG、HDL、LDL、GPT、GOT及胰岛素水平,肝脏组织切片进行病理学及细胞凋亡观察,进一步分析大鼠肝功能、脂质代谢、胰岛素抵抗及肝细胞凋亡对肝组织病理改变的影响.结果 模型组大鼠4周后就出现肝功能损伤,脂质代谢紊乱、胰岛素抵抗,肝细胞凋亡8W后明显增加,肝细胞脂变及炎症为肝组织病理变化的主要特征,且造模时间越长,病变程度越严重.结论 经过高脂饲料的喂养,SD大鼠在4～16周内可形成病变程度逐步加重的NAFLD模型,肝功能损伤,脂质代谢紊乱及肝细胞凋亡是引起非酒精性脂肪肝中脂肪变性和炎症的重要因素,该模型可应用于脂肪肝治疗药物评价等方面.     

Solid-phase peptide synthesis at elevated temperatures: a search for an optimized synthesis condition of unsulfated cholecystokinin-12

A systematic investigation of solid-phase peptide synthesis at elevated temperatures using the well-known aggregating peptide acyl carrier protein (65–74) and the unsulfated cholecystokinin-8 as models is presented. The main goal of the investigation was the determination of an optimized experimental condition for the synthesis of unsulfated cholecystokinin-12. Of the elevated temperatures used, 60°C was the most appropriate. The efficiency of N, N'-diisopropylcarbodiimide/l-hydroxybenzotriazole (DIC/HOBt) in 25% dimethyl sulfoxide (DMSO)/toluene at this temperature was similar to that of 2-(1-H-benzotriazole-1-yl)-1, 1, 3, 3-tetramethyluronium tetrafluoroborate (TBTU). Interestingly, this coupling reagent was more efficient than TBTU, benzotriazol-1-yl oxy-tris(dimethylamino)phosphonium and O-(7-azabenzotriazol-1-yl)-1, 1, 3, 3-tetramethyluronium hexafluorophosphate in N-methylpyrrolidone. 25%DMSO/toluene proved to be suitable for the swelling of the resins phenylacetamidomethyl, methylbenzhydrylamine, hydroxymethylphenoxy, 4-(benzyloxy)-2′, 4′-dimethoxybenzhydrylamine, 4-(2′, 4′-dimethoxyphenyl-Fmoc-aminomethyl)phenoxy and (4-succinylamido-2′, 2′, 4′-trimethoxy)benzhydrylamine. Those polymeric supports were fully compatible with the approach. Under the optimized synthesis condition found in these studies (temperature of 60°C, DIC/HOBt as coupling reagent and 25%DMSO/toluene as solvent), no difficulties related to the aggregation phenomenon were encountered. These data confirm the usefulness of solid-phase peptide synthesis at elevated temperatures and extend its applicability.     

Airflow measurement inaccuracies in aerosol imaging

Aerosol production using inclined compressed air tanks may be subject to error caused by airflow meter variability and by the degree of inclination of the air-flow meter. Since most of these tanks are used in an inclined position, it is important for clinicians to be aware of these errors.     

Transoral approach to the cervical spine: report of four cases

The transoral approach to the upper cervical spine is an established but little used route, offering excellent access with good wound healing, to lesions of the bodies of the atlas, axis and upper part of the third cervical vertebra. The authors report four cases which demonstrate the value of the procedure.