Anti-osteoporosis effect of the standard extract of Pueraria Lobata

Objective The standard extract of Pueraria Lobata(SEP)was extracted from the traditional Chinese medicine Pueraria Lobata.and the anti-osteoporotic effect of SEP were evaluated in the paper.Methods The ovariotomied rats were used simulating osteoporosis model,and characterized from thighbone measurement,bone density,blood biochemistry assay,emiction biochemistry,bone morphology and womb pathology were also determined.Results The ovariotomy causes rats' weight,serum ALP,Strap,bone calcium element,emiction calcium element,emiction hydroxide praline increased;bone calcium content,bone density and rigidity,thighbone wet weight,dry weight and ash weight remarkably reduced;and the bone anti-bend intensity,the maximum bend strength decreased,the bone girder thinner,or even be broken.This indicates that ovariotomy causes rats lost bone calcium and got osteoporosis.One month after operation,SEP was provided subsequently for 12 weeks to different samples(medicament quantity 2.5,1.25,0.625 g·kg-1;model group;fake operational group and masculine medicament estrogen group).It was found that SEP can suppress the weight increase caused by ovariotomy,improved the thighbone metrology standard of the OVX rats(the bone calcium content 26.2%,20.1% and 15.7% separately);increase the thighbone density and rigidity,the thighbone wet weight,dry weight and ash weight;increased blood calcium,blood phosphorus,decreasing ALP,Strap,increasing serum estradiol level,reducing bone calcium content;improve the emiction biochemistry characteristics:decreased emiction calcium,emiction hydroxide proline ejection amount;improved the OVX rats thighbone biomechanics characteristic:increased the anti-band intensity and maximum bend strength.Conclusions SEP exerted upon an inhibited effects on osteoporosis caused by ovariotomy.     

Ordered water and ligand mobility in the HIV-1 integrase-5CITEP complex: a molecular dynamics study.

A 2 ns molecular dynamics simulation has been carried out for the HIV-1 integrase-5CITEP complex in order to understand the role of water in defining the ligand's binding mode and to address issues of binding site flexibility and ligand motion. Although the ligand retains considerable mobility within the active site, a structural water molecule bridging 5CITEP with Asp 64 and Asn 155 is identified in the simulation. Consideration of this water molecule could open a route to new HIV-1 integrase inhibitors.     

肾肿瘤中浸润性树突状细胞的免疫状态变化

目的 通过分析。肾肿瘤中浸润性树突状细胞的免疫状态变化，以探讨其在。肾肿瘤微环境中的功能状态。方法选择未经任何非手术治疗的。肾透明细胞癌患者标本40例作为实验组，10例正常。肾组织作为对照组，通过免疫组织化学技术标记组织中树突状细胞（dendritic cell，DC）的CDla、HLA-DR和CD86抗原，观察正常。肾及。肾透明细胞癌组织中DC的3种抗原的表达，结果进行统计学分析。站果所有。肾透明细胞癌组织中均未见明显CD86＋ DC，但均有CDla＋ DC，其在肾癌组织中的浸润程度明显高于正常。肾组织（P〈O．001），但CDla＋ DC与肾癌临床分期及组织学分级均无相关性；在肾癌组中有34例癌实质内的DC表达HLA-DR抗原，其阳性表达率为85％，与正常肾组织80％的阳性表达率相比无统计学意义（P〉O．05）。站论DC具有趋化性，可以向肿瘤组织聚集，肾癌组织中存在CDIa，肾癌患者CD86表达低下，提示肾癌患者DC存在免疫缺陷，而DC的免疫功能低下则可能是肾癌逃避宿主免疫监视的主要原因。     

抗骨肉瘤单克隆抗体在骨肉瘤组织中的免疫组化定位研究

本文报道了用本室建立的2株抗人体骨肉瘤细胞株所分泌的单克隆抗体,采用ABC酶标法,对24种肿瘤组织和正常人体组织进行了免疫组化定位研究,发现OS-McAb_1和OS-McAb_2除对部分骨源性恶性肿瘤有阳性反应外,对其他部位的肿瘤均显示阴性反应;与所标记的正常成人及胎儿相应组织无交叉反应。提示OS-McAb对骨肉瘤有较高的特异性,在骨源性恶性肿瘤的免疫诊断方面有一定应用价值。     

膀胱癌患者外周血树突状细胞免疫状态变化及其临床意义

目的探讨膀胱癌患者外周血中树突状细胞（DC）的免疫功能状态变化及其与膀胱癌发生的关系。方法检测15例临床确诊的中晚期膀胱癌患者外周血DC表面CD1a、CD83表达水平及免疫诱导T淋巴细胞增殖的能力，并与10名健康成人比较。结果膀胱癌患者DC表面CDla及CD83表达水平（分别为57．4±1．6，72．1±1．1）明显低于正常对照组（64．9±1．4，80．6±1．2，P〈0．05）；其体外诱导T细胞增殖的能力亦明显低于正常对照组（P〈0．05）。结论膀胱癌患者DC存在免疫功能缺陷可能是膀胱癌逃避宿主免疫监视的重要原因之一。     

关于外加死腔和气流阻力复合负荷的心理生理效应研究

以20名健康男性青年为被试者,观察了外加死腔和呼吸阻力联合作用对呼吸功能和呼吸阻力感觉的影响。结果表明,在外加阻力条件下,外加死腔使反映通气功能和呼吸型式的参数值增加,且对反映呼吸功的参数具有相互增强的作用。当外加死腔增大时,呼吸阻力感觉阈限提高,相同的呼吸阻力值引起更强的呼吸感觉。上述结果讨设计制作呼吸防护装备具有参考价值。     

广西5个民族女性生理骨盆的对比研究

本文对广西壮、汉、仫佬、侗、瑶5个民族的500名妇女的生理骨盆进行了调查。调查内容包括临床、X线测量等47个项目。各项测量数据均在5个民族间进行两两比较。发现壮、汉两民族X线测量除入口前后径壮族略小于汉族外,其余各项指标无显著区别。而仫佬、侗、瑶3个民族又有其共同特点,如骨盆由入口→出口三个平面的横径、中部前后径、骨盆前部高度,3个民族间无明显区别,但此5条径线均明显大于壮、汉两民族。此外,仫佬、侗、瑶3民族的骶骨明显弯曲。骨盆入口形态后段呈扁形者5个民族的出现率均高,而仫佬、侗、瑶3民族骨盆入口前段呈猿形者又高于壮、汉两民族。     

“化不可代，时不可违”之我见

"化不可代,时不可违"语出《素问.五常政大论》。此语既是理论的,也是临床的;既具有时间康复学意义,又具有临床诊疗意义。它以中医"天人相应"观点为基础,揭示了人体气化与天地相参的本质,贯穿于疾病治疗、用药以及病后康复的始终。     

Changes in brain function and anatomical structure following treatment of hyperbaric oxygen for visual pathway abnormalities in 16 cases★ Evaluation of functional magnetic resonance imaging combined with diffusion tensor imaging

BACKGROUND： There is a growing research focus on the combination of blood oxygenation level dependent functional magnetic resonance imaging （BOLD-fMRI） and diffusion tensor imaging （DTI） to evaluate visual cortical function and structural changes in the cerebrum, as well as morphological changes to the white matter fiber tracks, after visual pathway lesions. However, the combined application of BOLD-fMRI and DTI in treating of visual pathway abnormalities still requires further studies.
OBJECTIVE： To observe and evaluate the effects of hyperbaric oxygen on visual pathway abnormalities, and to evaluate the characteristics of cerebral function and anatomic structural changes by using BOLD-fMRI combined with DTI technique. DESIGN： Case contrast observation.
SETTING： Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA.
PARTICIPANTS： Sixteen patients （9 males and 7 females, 15-77 years old） with lateral or bilateral visual disorder induced by visual pathway lesions were selected from the Department of Neurology, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA from January 2006 to May 2007. These patients comprised the lesion group. Measures of interventional therapy： hyperbaric oxygen of two normal atmospheres for three courses （10 d/course） and routine internal medicine treatment. In addition, 12 healthy subjects of similar sex and age to the lesion group were regarded as the control group. The control group underwent routine ophthalmological and ocular fundus examinations; diagnostic results were normal. The experiment received confirmed consent from the local ethic committee, and all patients provided informed consent.
METHODS： BOLD-fMRI and DTI manifestations in the lesion group were observed before and after hyperbaric oxygen intervention, and the results were compared with the control group. The subjects were positioned on their back, and BOLD-fMRI images were collected with the following GRE EPI sequence： TR = 2 000 ms, TE = 40     

羊皮覆盖微粒皮肤移植11例体会

目的 探讨异种羊皮作为创面覆盖物行微粒皮肤移植的临床应用。方法 微粒皮肤移植羊皮覆盖１１例。结果 微粒移植Ⅰ类愈合占５７．１％，Ⅱ类愈合占３５．７％。结论 羊皮作为烧伤同的覆盖物必须在保证活力的前提下使用，可以满足临床需要。