Left atrial appendage perforation with Watchman device implantation: Strategies for surgical repair

Percutaneous occlusion of the left atrial appendage is increasingly being used as an alternative for stroke prevention in patients with non‐valvular atrial fibrillation at high risk of complications from long term anticoagulation. We describe a case of left atrial appendage perforation during Watchman device implantation requiring emergency repair of the left atrium using sternotomy and cardiopulmonary bypass. Technical considerations for surgical decision making are discussed; in hemodynamically unstable patients as well as those at high risk for embolization.     

知识模式转换在医学科技创新中的作用

医学科技创新是医院赖以生存与发展的动力,医学科技创新的过程也是医学知识模式不断转换的过程,本文从知识模式转换的视角,探讨知识管理对医学科技创新的意义与作用     

三磷酸腺苷生物荧光液闪测定法检测活细胞计数

为建立一种检测活细胞数的方法,作者采用三磷酸腺苷(ATP)生物荧光液闪测定法检测小鼠纤维瘤细胞株L929.ATP标准曲线工作范围为10 -9～10-5mol/ml,相关系数r=0.9963(P＜0.001);当活细胞数在3×1 02～106个/ml时与发光计数值有良好的线性关系,r=0.9922(P＜0.001),变异系数 CV = 1%～3%.用ATP生物荧光液闪测定法检测活细胞数灵敏、简便、准确、重复性好,用液闪计数仪即可完成测定,有较大的实用价值.     

An adenosine triphosphate bioluminescence assay for detecting the number of living cells]

The method for detecting the number of living cells was studied. Using an adenosine triphosphate (ATP) bioluminescence assay, the present authors reported a perfect linear relationship between lg ATP concentrations and lg luminescence counts (r = 0.9963) as well as a relationship between lg number of cells and lg ATP luminescence counts (r = 0.9922). The detectable cells ranged from 10(2) to 10(6) cells/ml, the coefficients of variation 1-3%. This method is simple, accurate and sensitive and has a high reproducibility.     

Golytely肠道清洁法应用292例分析

本院自 1 993年初以来 ,用Ｇｏｌｙｔｅｌｙ液对2 92例结、直肠手术及纤维结肠镜检查病人作术前准备均取得较清单效果 ,现介绍如下。1　临床资料1 .1　 一般资料　本组患者共 2 92例 ,其中男性 1 85例 ,女性 1 0 7例。结、直肠手术 1 2 7例 ,诊断结肠肿瘤 72例 ,直肠肿瘤 5 5例。纤维结肠镜检查 1 6 5例。1 .2　配方及使用方法　Ｇｏｌｙｔｅｌｙ液配方〔1〕:聚乙二醇 40 0 0 5 9.1 ｇ,硫酸钠 5 .6 ｇ,氯化钠0 .75 ｇ,碳酸氢钠 1 .3ｇ加水至 1 0 0 0ｍｌ。使用方法 :术前 1ｄ下午服Ｇｏｌｙｔｅｌｙ ,饮速大于1 5 0 0ｍｌ/ｈ ,至排出清液止 …     

脑血管病危险因素在海口城乡对比的调查

目的 通过脑血管病危险因素在海口城乡对比的调查,了解脑血管病发病的相关因素。方法 以整群随机抽样法,从超过４０岁人群中取城市１９２人,农村２３１人进行测定血糖、血甭胆固醇、苷油三酯、高密度脂蛋白、低密度脂蛋白、极低密度脂蛋白。结果 经统计学处理,发现胆固醇、苷油三酯高、低密度脂蛋白、极低密度脂蛋白、极低密度脂蛋白城乡比较均有显著差异（Ｐ〈０．０１）,脑血管病发病率、死亡率,城市明显高于乡村（Ｐ〈０     

慢性重型肝炎124例临床分析

目的 通过１２４例慢重肝临床分析对上前慢重肝治疗疗效、临床难点作一简要分析。方法 １２４例慢重肝进行病原分型、临床分期、分组治疗及疗效判定。结果 ①早、中期慢重父瘟菩Ы暇捌谡呦灾圆钜欤ǎ小矗埃埃担＂谠纭⒅衅诼馗尾∷缆式贤砥谡哂邢灾差异（Ｐ〈０．０５）。③分组治疗病死率无显著性差异（Ｐ〉０．０５）。④死亡组与存活组病例ＴＢ、ＡＬＢ、ＰＴＡ有显著性差异（Ｐ〈０．０５）。结论 ①早、中期慢重肝     

U-Patch GAN: A Medical Image Fusion Method Based on GAN

Journal of Digital Imaging - Although medical imaging is frequently used to diagnose diseases, in complex diagnostic situations, specialists typically need to look at different modalities of image...     

人原发性肝细胞癌核基质蛋白的研究

目的比较正常肝与原发性肝细胞癌（ＨＣＣ）核基质蛋白的异同,观察是否有ＨＣＣ特异性核基质蛋白的存在。方法用双向电泳方法比较了３例正常肝和８例ＨＣＣ核基质蛋白成分。结果正常肝和ＨＣＣ的核基质蛋白组成极为相似,但在ＨＣＣ中发现至少有４个ＨＣＣ的特异性核基质蛋白。其中以分子量为６２０００、等电点为５．３的蛋白最具代表性,它存在于所研究的８例ＨＣＣ中。其余３个ＨＣＣ特异性蛋白亦存在于大多数ＨＣＣ中。３例正常肝组织中未见有这４个ＨＣＣ特异性核基质蛋白。结论ＨＣＣ确实有ＨＣＣ特异性核基质蛋白的存在,它的发现可能会为ＨＣＣ的发生、发展、发病机理的研究提供一个新途径。     

Sialylated human apolipoprotein E (apoEs) is preferentially associated with neuron-enriched cultures from APOE transgenic mice

Mice transgenic for human APOE2, E3, and E4 alleles express native 34-kDa human apoE and two sialylated apoE isoproteins with approximate molecular weights of 37 kDa (apoEs) and 39 kDa (apoEs2) in brain. These multiple apoE/apoEs/apoEs2 band patterns on Western blot are also observed in human brain, but are not seen in wild-type mouse brain. Both the 37-kDa apoEs and 39-kDa apoEs2 are coprecipitated with native 34-kDa apoE by antibody to human apoE. Neuraminidase digestion eliminates the 37- and 39-kDa forms and results in a downward shift in the bands to the position of the 34-kDa native form. These sialylated apoE isoproteins are found preferentially associated with neurons and contribute significantly (50-60%) to the total neuronal apoE in neuronal cultures from transgenic mice, while only 5-10% of total apoE is sialylated in cultures enriched in glial cells. In situ hybridization and immunocytochemistry demonstrate apoE mRNA and apoE immunoreactivity are predominantly located in cell soma of neurons, not in neuronal processes.