Reanalysis of microRNA expression profiles identifies novel biomarkers for hepatocellular carcinoma prognosis

The aim of our study is to identify microRNAs (miRNAs) that have significance in the prognosis and pathogenesis of hepatocellular carcinoma (HCC). The miRNAs differentially expressed in HCC were examined by using a human miRNA microarray dataset, and then the acquired candidates were screened by another microarray dataset. As a result, we got 25 miRNAs which were aberrantly expressed in cancer and meanwhile predicated distinct prognosis. Among them, miR-139-5p was down-regulated in HCC and its low expression in cancer tissue meant poor prognosis. Additionally, we demonstrated that its low expression was also related to several clinicopathologic characteristics such as vein invasion, BCLC stage, p-AKT expression, and pIGFR1 expression. In vitro, it has been discovered that treatment of HCC cells with a miR-139-5p mimic lead to inhibition of cell growth and migration. Moreover, luciferase assay showed that KPNA4 was not the direct target of miR-139-5p. Ectopic expression of miR-139-5p has not repressed the expression of KPNA4, but inhibited the nuclear import of NF-κB and phosphorylation of Akt. In conclusion, for the first time, we identify 25 deregulated miRNAs that are associated with prognosis and prove that miR-139-5p functions as a tumor suppressor in HCC and its low expression predicts poor prognosis.

     

天晴甘美对慢性乙型肝炎患者外周血单个核细胞细胞周期的影响简

目的：研究天晴甘关注射液对慢性乙型肝炎（慢乙肝）患者外周血单个核细胞（PBMC）细胞周期的影响。方法：采用凯基细胞周期检测法检测天晴甘关对细胞周期的影响。结果：天晴甘美注射液呈剂量依赖性抑制外周血单个核细胞z增殖,使细胞阻滞于G。／G。期,细胞DNA合成减少,但细胞无凋亡。结论：天晴甘美注射液对PBMC具有一定抑制作用,可能使细胞阻滞于G。／G。期,导致细胞DNA合成减少。     

快速分光光度法测定含蛋白质高的冷饮食品中糖精

研究了用天青Ｂ测定含蛋白质高的冷饮食品中糖精的分析方法．该法不需昂贵设备．快速，简便而准确，来自６家食品厂的９个不同样品中测定糖精的平均回收率为９７．６％．，奶粉、鲜蛋、小豆的公限度分别为８０ｇ／Ｌ，７０ｇ／Ｌ，５０ｇ／Ｌ．     

Activation of β-catenin signaling is critical for doxorubicin-induced epithelial–mesenchymal transition in BGC-823 gastric cancer cell line

The epithelial–mesenchymal transition (EMT) is a fundamental process governing morphogenesis in multicellular organisms and has recently been implicated in promoting carcinoma invasion and metastasis. Besides their therapeutic effects, accumulating evidences suggest that chemotherapeutic agents also induced EMT and enhanced the malignancy of treated cancer cells; however, the mechanism(s) still remains unclear. Here, we investigated the role of β-catenin signaling in doxorubicin (Dox)-induced EMT in human gastric cancer cell line BGC-823. We found that the transient treatment of Dox induced EMT and enhanced the in vitro migration ability of cancer cells. We also found that β-catenin signaling was activated upon Dox treatment. Inhibition of β-catenin by indomethacin (Indo) or siRNA suppressed Dox-induced EMT and decreased cancer cell migration ability. Our results showed that β-catenin signaling was critical to Dox-induced EMT. Indo and other β-catenin inhibitors may have a potential implication in prevention of gastric cancer metastasis.     

TLR7激活对HepG2.2.15细胞株炎症因子TNF-α和IL-6表达的上调作用

目的比较乙型肝炎病毒(hepatitis B virus,HBV)基因组稳定转染细胞株HepG2.2.15和肝癌细胞株HepG2中TLR7表达,分析HepG2.2.15细胞中TLR7激活后诱导炎症因子表达。方法通过实时荧光定量(Real-time)PCR分析HepG2和HepG2.2.15中TLR7受体、IL-6和TNF-αmRNA水平表达。TLR7配体Gardiquimod刺激20min后,Western blot分析激活的信号通路;刺激6h,Real-timePCR分析TLR7激活后IL-6和TNF-αmRNA水平表达变化;刺激48h,ELISA法分析IL-6和TNF-α蛋白质水平表达变化。结果HepG2.2.15细胞株中TLR7mRNA水平表达比HepG2中的表达高约30倍(P0.01)。在没有任何刺激的条件下,HepG2.2.15细胞株中IL-6和TNF-αmRNA水平表达比HepG2中的表达分别高约7.5和9.3倍;Gardiquimod刺激6h后,HepG2.2.15细胞株中IL-6和TNF-αmRNA表达水平明显升高,并具有剂量依赖关系(P0.01),而HepG2细胞在相同的刺激下IL-6和TNF-αmRNA表达水平升高并不明显(P0.05);刺激48h后,IL-6和TNF-α蛋白质表达水平变化与其mRNA水平变化一致(P0.01)。HepG2.2.15细胞株经Gardiquimod刺激后NF-κBp65亚单位进入细胞核中量显著增加。结论TLR7激活后能够上调HepG2.2.15细胞中IL-6和TNF-α的表达,提示TLR7可能在乙型病毒性肝炎的病理生理过程中发挥作用。     

FLT3-ITD突变与附加染色体核型对急性早幼粒细胞白血病患者预后的影响

目的 探讨FLT3-ITD突变与附加染色体核型对急性早幼粒细胞白血病(APL)患者预后的影响。 方法 回顾性分析2012年1月～2021年3月安徽医科大学第二附属医院新诊断为APL的患者112例,收集患者临床和实验室资料,根据危险度分层选择治疗方案,通过电话或查看门诊或住院病历来完成随访总生存(OS)。结果 APL患者合并FLT3-ITD基因突变28例(25.0%),有附加染色体核型患者19例(16.9% ),早期死亡(ED) 9例(8.0% )。有无FLT3-ITD突变患者完全缓解率(CR)差异无统计学意义（P＞0.05）;ED及高危分层无明显相关性（P＞0.05）。有无附加染色体核型患者CR率差异无统计学意义（P＞0.05）,与ED 及高危分层无明显相关性（P＞0.05）。中位随访68.5（0.1～108.0）月,总体生存率80.4％,有无高危FLT3-ITD突变的生存率差异无统计学意义（P＞0.05）。有无附加染色体核型患者的生存率分别为78.9％、80.6％（P＞0.05）。结论 FLT3-ITD突变与附加染色体核型对APL患者长期生存没有影响。     

Feasibility evaluation of micro-optical coherence tomography (μOCT) for rapid brain tumor type and grade discriminations: μOCT images versus pathology

Central skull-base osteomyelitis (CSBO) represents a life-threatening complication of external ear canal infection. Computed tomography (CT) and magnetic resonance imaging (MRI) play key roles in assessment of CSBO progression. Twelve patients with CSBO were included in a retrospective clinical study. In total, 62 scans (30 CTs and 32 MRIs) were performed to evaluate the extent of inflammatory changes. The scans were read independently by two radiologists specialised in imaging of the head and neck. The regions under the skull base were specified using the online Anatomy Atlas of the skull base. To clarify the timeline, the time period was divided into four parts, and inflammatory changes in the skull-base regions were tracked. Data were statistically analysed. In early stages of the disease, CT scan detects inflammatory changes closely related to the stylomastoid foramen and medially to the posterior belly of the digastric muscle, changes which have been proved to be crucial for the diagnosis of CSBO. Later the infection spreads to the contralateral side causing demineralisation of the bones. Imaging methods play a crucial role not only in establishing the diagnosis, but also in anticipating the direction of infection spread underneath the skull base.     

人工智能辅助低年资放射科医师检出乳腺病变

目的观察人工智能(AI)辅助对低年资放射科医师检出乳腺X线片中乳腺病灶的价值。方法回顾性分析73例接受乳腺X线摄影检查的女性患者。由3名低年资放射科医师首先分别阅片,之后于AI辅助下再次阅片,勾画乳腺病灶区域;统计检出病灶数,记录病灶类型及乳腺影像报告和数据系统(BI-RADS)分类。以病理结果或随访最终临床诊断为标准,对比医师单独阅片与AI辅助阅片检出乳腺病灶的敏感度和准确率及对不同类型、不同BI-RADS分类病灶的诊断效能。结果 73例共121个乳腺病灶,其中软组织病灶(包括肿块、结构扭曲与不对称病灶)88个、钙化病灶33个;BI-RADS 2类17个,3类66个,4类及以上38个。与医师单独阅片相比,AI辅助阅片对全部乳腺病灶、特别是软组织病灶的诊断敏感度和准确率均显著提升(P均0.01),而对乳腺钙化病灶的敏感度和准确率差异均无统计学意义(P均0.05)。结论 AI辅助有助于提高低年资放射科医师检出乳腺X线片中乳腺病灶、尤其软组织病灶的效能。     

Clinical and Experimental Study on Treatment of Chronic Hepatitis B with Yanggan Aoping (养肝澳平) Mixture

CLINICALSTUDYOlinlcalDaisDiagnosisforchronichepatitisBwasperformedaccordingtothecriteriaofthepreventionandtreatmentofviralhepatitis,whichwasmadeatShanghaiconferencein1990(1).Inthetreatmentgroup,therewere152patients,including114malesand38females,agedfrom11to58yearsold.Themeanagewas31.46110.52yearsold.Inthesecases,79caseswerechronicpersistenthepatitis(CPH)withHBeAg-POsitive.Theother73caseswerechronicactivehepatitis(CAH),40casesofthemwereHBeAgPOsitive.Inthecontrolgroup,therewere100c…