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81.
82.
Arianna Aprile Giovanna Palermo Antonio De Luca Roberta Pinalli Enrico Dalcanale Pasquale Pagliusi 《RSC advances》2018,8(29):16314
Reliable chemical sensors with high selectivity and sensitivity toward specific target molecules require rational synthesis of receptors, in-depth characterization of their complexation abilities and highly efficient transduction of the molecular recognition event. Here we report a steady-state and time-resolved fluorescence investigation of EtQxBox, a fluorescent conformationally blocked quinoxaline-based cavitand, aimed at assessing its selectivity toward aromatic versus non-aromatic analytes in solution. Fluorescence quenching of the EtQxBox in acetone is observed at increasing concentration of both aromatic (i.e. benzonitrile) and aliphatic (i.e. acetonitrile) compounds. The combination with fluorescence lifetime measurements permits to discriminate the predominantly static quenching of the aromatic analyte, due to non-fluorescent host–guest complex formation, from the mostly dynamic quenching of the non-aromatic compound, resulting from aspecific diffusive collisions between the fluorophore and the quencher. The equilibrium association constants for both the complexes have been estimated using Stern–Volmer model.We investigate the role of combined static and dynamic quenching in fluorescence transduction of benzonitrile and acetonitrile complexation by a rigid quinoxaline-based cavitand. 相似文献
83.
Piers Blombery Lucy C. Fox Georgina L. Ryland Ella R. Thompson Jennifer Lickiss Michelle McBean Satwica Yerneni David Hughes Anthea Greenway Francoise Mechinaud Erica M. Wood Graham J. Lieschke Jeff Szer Pasquale Barbaro John Roy Joel Wight Elly Lynch Melissa Martyn Clara Gaff David Ritchie 《Haematologica》2021,106(1):64
Bone marrow failure (BMF) related to hypoplasia of hematopoietic elements in the bone marrow is a heterogeneous clinical entity with a broad differential diagnosis including both inherited and acquired causes. Accurate diagnostic categorization is critical to optimal patient care and detection of genomic variants in these patients may provide this important diagnostic and prognostic information. We performed real-time, accredited (ISO15189) comprehensive genomic characterization including targeted sequencing and whole exome sequencing in 115 patients with BMF syndromes (median age 24 years, range: 3 months - 81 years). In patients with clinical diagnoses of inherited BMF syndromes, acquired BMF syndromes or clinically unclassifiable BMF we detected variants in 52% (12 of 23), 53% (25 of 47) and 56% (25 of 45) respectively. Genomic characterization resulted in a change of diagnosis in 30 of 115 (26%) including the identification of germline causes for 3 of 47 and 16 of 45 cases with pre-test diagnoses of acquired and clinically unclassifiable BMF respectively. The observed clinical impact of accurate diagnostic categorization included choice to perform allogeneic stem cell transplantation, disease-specific targeted treatments, identification of at-risk family members and influence of sibling allogeneic stem cell donor choice. Multiple novel pathogenic variants and copy number changes were identified in our cohort including in TERT, FANCA, RPS7 and SAMD9. Whole exome sequence analysis facilitated the identification of variants in two genes not typically associated with a primary clinical manifestation of BMF but also demonstrated reduced sensitivity for detecting low level acquired variants. In conclusion, genomic characterization can improve diagnostic categorization of patients presenting with hypoplastic BMF syndromes and should be routinely performed in this group of patients. 相似文献
84.
Valerio Rosato Antonio Ascione Riccardo Nevola Anna Ludovica Fracanzani Guido Piai Vincenzo Messina Ernesto Claar Carmine Coppola Luca Fontanella Rosa Lombardi Laura Staiano Giovanna Valente Maria Chiara Fascione Chiara Giorgione Annalisa Mazzocca Raffaele Galiero Pasquale Perillo Aldo Marrone Ferdinando Carlo Sasso Luigi Elio Adinolfi Luca Rinaldi 《Journal of viral hepatitis》2022,29(1):26-34
The long-term changes of liver stiffness (LS) in patients who achieve viral clearance after direct-acting anti-HCV therapy remain undefined. We conducted a multicentre prospective study to investigate this aspect. Patients with HCV infection treated with DAAs were enrolled from six Italian centres; they underwent clinical, biochemical, ultrasound and transient elastography evaluations before treatment (T0), 12 weeks (SVR12) and 24 months (T24) after the end of therapy. Among the 516 consecutive patients enrolled, 301 had cirrhosis. LS significantly decreased from T0 to SVR (14.3 vs 11.1 kPa, p = .002), with a progressive reduction until T24 (8.7 kPa, p < .001). However, only patients with steatosis and those who developed HCC did not experience a late improvement in LS. Multivariate analysis of baseline and follow-up variables identified steatosis as the only independent predictor of failure of LS improvement (OR 1.802, p = .013). ROC curve analysis of the association of LS with the risk of developing HCC showed that SVR12 ≥14.0 kPa had the highest accuracy (sensitivity 82%, specificity 99%; AUC: 0.774). Multivariate analysis revealed that LS was the only variable independently associated with an increased risk of developing HCC (OR 6.470, p = .035). Achieving an SVR was associated with a progressive, long-term decline of LS, suggesting a late improvement in liver fibrosis, besides the resolution of inflammation. Fatty liver and the development of HCC interfered with late reduction of LS. Patients with an LS ≥14 kPa at 12 weeks after the end of treatment were at higher risk for developing HCC. 相似文献
85.
Pelargonio Gemma Di Monaco Antonio Guida Pietro Pellegrino Pier Luigi Vergara Pasquale Grimaldi Massimo Narducci Maria Lucia Tritto Massimo 《Journal of interventional cardiac electrophysiology》2022,63(1):125-132
Journal of Interventional Cardiac Electrophysiology - Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia, occurring in 1–2% of the general population. Catheter ablation... 相似文献
86.
Naga Venkata K. Pothineni MD Nigel Amankwah MD Pasquale Santangeli MD PhD Robert D. Schaller DO Gregory E. Supple MD Rajat Deo MD Saman Nazarian MD Fermin C. Garcia MD Sanjay Dixit MD David J. Callans MD Francis E. Marchlinski MD David S. Frankel MD 《Journal of cardiovascular electrophysiology》2021,32(2):345-353
87.
Pasquale Vergara MD PhD Carlo Pignalberi MD Ennio C. Pisanò MD Giampiero Maglia MD Paolo Della Bella MD Gabriele Zanotto MD Saverio Iacopino MD Francesco Solimene MD Valeria Calvi MD Massimiliano Marini MD Massimo Giammaria MD Mauro Biffi MD Giovanni Rovaris MD Fabrizio Caravati MD Fabio Quartieri MD Antonio Curnis MD Antonio Rapacciuolo MD PhD Gaetano Senatore MD Stefano Pedretti MD Davide Saporito MD Antonio Dello Russo MD Vincenzo E. Santobuono MD PhD Patrizia Pepi MD Antonio Duca MD Matteo Baroni MD Giulio Falasconi MD Daniele Giacopelli MSc Alessio Gargaro MSc Antonio D'Onofrio MD 《Journal of cardiovascular electrophysiology》2021,32(9):2528-2535
88.
Sorvillo F Mazziotti G Carbone A Piscopo M Rotondi M Cioffi M Musto P Biondi B Iorio S Amato G Carella C 《The Journal of clinical endocrinology and metabolism》2003,88(10):4818-4822
In this study, we have investigated in vivo the time-dependent effects of TSH on vascular endothelial growth factor (VEGF) production in patients monitored for thyroid carcinoma. Serum VEGF, thyroglobulin (Tg), and TSH levels were assayed at baseline and 6, 24, 30, 48, 72, and 96 h and 1 wk after administration of recombinant human TSH (rhTSH) in 45 thyroidectomized patients affected by differentiated thyroid carcinoma. At baseline, the patients with metastasis (18 cases) showed serum Tg and VEGF values significantly higher than those seen in the cured patients (27 cases). During rhTSH stimulation, the mean VEGF levels decreased significantly in both patient groups. In 60% of patients with metastasis, VEGF nadir occurred at the same time as serum TSH reached the highest values, whereas in 85.7% of the cured patients VEGF decreased after the TSH peak (P = 0.003). In conclusion, we demonstrate for the first time that short-term administration of rhTSH in patients monitored for differentiated thyroid carcinoma induces a significant reduction in serum VEGF values even in the absence of thyroid tissue. This result would suggest that TSH may be able in vivo to regulate VEGF production from tissues other than the thyroid gland. 相似文献
89.
Andrea Rubboli Mauro Colletta Pietro Sangiorgio Giuseppe Di Pasquale 《Italian heart journal》2004,5(11):851-856
BACKGROUND: In the absence of evidence-based data, the optimal antithrombotic treatment after coronary artery stenting in patients on chronic oral anticoagulation (OAC) remains unknown. In order to investigate current practice in this setting, an international survey was carried out. METHODS: A questionnaire was e-mailed to 40 internationally renowned, foreign Interventional Centers worldwide. RESULTS: Out of the 24 Centers (60%) replying, only in 13 (54%) is antithrombotic treatment carried out in accordance with a standardized protocol. OAC is stopped in favor of aspirin plus ticlopidine/clopidogrel in selected (low thromboembolic risk) conditions in 13 (54%) Centers. When OAC is continued, the association with a single antiplatelet is employed in a few Centers only, as opposed to triple antithrombotic treatment (OAC and aspirin plus ticlopidine/clopidogrel) which is adopted, selectively or systematically, in the majority (83%) of Centers. In 8 (33%) Centers adopting triple antithrombotic treatment, the dose of OAC is decreased in all patients, whereas in 9 (38%) it is left unchanged. Upon completion of 1 to 3-6 months of antithrombotic treatment with OAC and single/dual antiplatelets, in 9 (38%) Centers this regimen is continued indefinitely, whereas in 10 (41%) antiplatelets are systematically withdrawn. Out of the 13 Centers, selectively exchanging OAC for aspirin plus ticlopidine/clopidogrel, low- or full-dose low-molecular-weight heparin is added in selected (high thromboembolic risk) cases in 3 (23%) and 5 (38%) Centers, respectively. Following 1 to 3-6 months of aspirin plus ticlopidine/clopidogrel antithrombotic treatment, OAC is resumed in all cases in 9 (69%) Centers and in no cases in 1 (8%). CONCLUSIONS: Our survey shows a high variability in the current antithrombotic treatment of patients on chronic OAC undergoing coronary artery stenting. Although various regimens may be adopted, the optimal antithrombotic treatment for this patient subset still needs to be identified. 相似文献
90.
Rossi S Fugazzola L De Pasquale L Braidotti P Cirello V Beck-Peccoz P Bosari S Bastagli A 《Endocrine-related cancer》2005,12(2):281-289
We report the simultaneous occurrence of medullary thyroid carcinoma (MTC) and papillary thyroid carcinoma (PTC), presenting as spatially distinct and well-defined tumour components, in three cases. In the first patient, histology, immunohistochemistry and electron microscopy demonstrated an MTC in the one nodule and PTC in two additional lesions. Non-neoplastic thyroid parenchyma separated the three nodules. Metastasis from PTC was diagnosed in a regional lymph node. Genetic analysis of both tumour components showed a distinctive mutational pattern: in the MTC a Cys634Arg substitution in exon 11 of the RET gene and in the two PTC foci a Val600Glu substitution in exon 15 of the BRAF gene. The other two patients are members of a large multigenerational family affected with familial MTC due to a germline mutation of the RET gene (Ala891Ser). Both patients harboured, besides medullary cancer and C-cell hyperplasia, distinct foci of papillary thyroid cancer, which was positive for Val600Glu BRAF mutation. Review of the literature disclosed 18 similar lesions reported and allowed the identification of different patterns of clinical presentation and biological behaviour. So far, the pathogenesis of these peculiar cases of thyroid malignancy has been completely unknown, but an underlying common genetic drive has been hypothesised. This is the first report in which two mutations, in the RET and BRAF genes, have been identified in three cases of MTC/PTC collision tumour, thus documenting the different genetic origin of these two coexisting carcinomas. 相似文献