Immunologic heterogeneity of diffuse large cell lymphoma

The cellular lineage of 57 diffuse large-cell lymphomas (DLCLs) was determined using a panel of monoclonal antibodies directed against lineage-restricted and -associated T, B, and monocyte antigens. The majority (82%) were of B cell lineage as determined by the expression of sig and/or B1, with the remaining 16% being of T cell lineage and 2%, of monocyte-myeloid lineage. By the expression of other B cell- restricted and -associated antigens, two major and two minor subgroups could be identified. These subgroups expressed the following phenotypes: (1) B1+B4+sIG+B2- (51%); (2) B1+B4+sIg+B2+ (29%); (3) B1+B4+sIg-B2+ (10%); and (4) B1+B4-sIg+B2- (10)%. The morphology of transformed lymphocytes, the weak to absent expression of the early B cell antigens B2 and sIgD, and the absence of the late B cell differentiation antigens PCA-1 and PC-1 suggested that these tumors were the neoplastic counterparts of normal B cells at the mid-stages of differentiation. Further support for the notion that B-DLCLs correspond to transformed B lymphocytes was concluded from the observation that B cells could be identified in normal spleen that expressed the cell surface phenotype and morphological appearance of the majority of B- DLCLs.     

心包积液的诊断──磁共振成像与超声心动图的对照研究

作者叙述了２４例心包积液（ＰＥ）的ＭＲＩ所见，并同超声心动图（ＵＳ）检查结果进行了对比分析，研究结果表明：ＭＲＩ与ＵＳ均为诊断ＰＥ的有效手段，可用于ＰＥ的半定量分析。ＭＲＩ发现少量ＰＥ较ＵＳ敏感，能从多体位更准确地显示ＰＥ的部位、分布等，同时对积液性质的判断也有一定的帮助。     

增生性瘢痕和瘢痕疙瘩组织中TGF—β1及Ⅰ,Ⅲ型胶原基因 …

目的 检测增生性瘢痕（Ｈ）和瘢痕疙瘩（Ｋ）组织中ＴＧＦ－β１及Ⅰ、Ⅲ型前胶原ｍＲＮＡ的表达,了解其相互关系及意义。方法 斑点杂交分析检测Ｈ和Ｋ组织中Ⅰ、Ⅲ前胶原及ＴＧＦ－β１ｍＲＢＡ稳态水平的改变;原位杂交检测ＴＧＦ－β１ｍＲＮＡ在组织中的空间分布。结果 ①Ｋ和Ｈ组织中ＴＧＦ－β１ｍＲＮＡ稳态水平明显高于Ｎ组和Ｓ组;②Ｋ选择性Ｉ型前胶原ｍＲＮＡ表达增强,而Ｈ组织中Ⅰ、Ⅲ前胶原ｍＲＮＡ表达均增强。     

高湿环境下某地驻训官兵心理健康状况调查

目的 初步了解高湿环境下部队官兵心理健康状况.方法 同〈高湿环境下患病中医症候分布调查分析〉一文.结果 高湿环境下,部队官兵SCL-90量表9个维度得分均显著低于国人常模,女性官兵的躯体化、强迫症状、恐怖、偏执和精神病性5个维度得分高于男性官兵（P＜0.05）,患有任意中医症候的官兵SCL-90各维度得分均高于无中医症候的部队官兵（P＜0.05）.结论 高湿环境下,男性官兵的心理健康状况优于女性官兵;无中医症候的官兵心理健康状况优于患有任意中医症候的官兵.     

原发性肝癌肝动脉化疗栓塞联合生物治疗的价值

目的 评价肝动脉化疗栓塞联合生物治疗对原发性肝癌的疗效。方法 56例原发性肝癌被随机分为两组:接受肝动脉化疗栓塞联合生物治疗的28例为观察组,只接受肝动脉化疗栓塞的28例为对照组。两组在治疗后半年、1年、2年的疗效与生存率分别作X~2检验,进行对照分析。结果 观察组的总有效率为67.8％(19/28)。而对照组为53.6％(15/28),两组间无显著性差异(X~2=1.20,P>0.05);半年、1年、2年时的生存率观察组分别为92.9％(26/28)、39.3％(11/28)和14.3％(4/28),而对照组分别为71.4％(20/28),14.3％(4/28)和0,两组分别比较有显著性差异(X~2值分别为4.38,4.46,4.30,P值均小于0.05)。结论 就原发性肝癌的保守治疗而言,肝动脉化疗栓塞联合生物治疗的效果期明显优于单纯肝动脉化疗栓塞。     

经尿道等离子前列腺剜除术与电切术治疗高危良性前列腺增生的比较

【摘要】 目的 探讨经尿道等离子前列腺剜除术(TUPKEP)与经尿道等离子前列腺电切术(TUPKRP)治疗高危良性前列腺增生(HrBPH)的安全性和有效性比较。方法 回顾性分析128例高危BPH 患者接受TUPKEP及TUPKRP两种手术方式,就两组患者的手术时间、出血量、前列腺腺体切除量、术后膀胱冲洗时间、尿管停留时间、术后住院天数、手术并发症等进行比较;对两组患者术前、术后国际前列腺症状评分(IPSS)、生活质量评分(QOL)、残余尿量(PVR)、最大尿流率(Qmax)进行比较。结果 68例行TUPKEP,60例行TUPKRP,所有128例患者安全度过围手术期。两组患者术前情况比较无统计学差异;两组患者手术后的QOL、PVR、IPSS、Qmax与各自术前比较,其差异有统计学意义(P<0.01),但上述指标在两组之间比较的差异并无统计学意义(P>0.05)。TUPKEP组在手术的出血量、手术时间、术后膀胱冲洗时间以及术后的住院时间要明显小于TUPKRP组(P<0.05);同时,TUPKEP组前列腺腺体切除量也高于TUPKRP组(P<0.05)。结论 TUPKEP 与TUPKRP在治疗高危BPH中,二者的临床疗效相当,但TUPKEP在手术时间、术后膀胱冲洗时间和住院时间较TUPKRP短,而且出血量和并发症均较少少,是治疗高危良性前列腺增生症的安全有效的方法。     

Computer graphics for digitally formatted images

The increasing use of digitally formatted imaging systems requires high-quality interactive gray-scale computer raster graphics systems for the management, display, and analog film recording of digital image and alphanumeric information. These systems are a combination of computer hardware and software and implement a set of graphics protocols. This paper describes a set of interactive graphics protocols that has been developed for clinical use.     

Expression of inducible nitric oxide synthase in muscle flaps treated with ischemic postconditioning

Background/Objective

Preconditioning has been considered promising for the treatment of ischemic flaps. In this study, the therapeutic effect of postconditioning was compared with that of preconditioning during ischemia/reperfusion (I/R) injury, and a role of inducible nitric oxide synthase (iNOS) in postconditioning treatment was also explored.

Methods

Sixty rats were randomly divided into four groups with 15 rats in each group. Ischemic injury was induced in a rat’s gracilis muscle flap model. Preconditioning and postconditioning were performed respectively on the flaps in the pre-con group and the post-con group. No treatment was given to the flaps in the control group, and flaps without I/R injury were used as a sham control. Muscle viability ratio, histology, and gene expression of iNOS were examined at different time intervals (3, 12, and 18 h).

Results

A significantly higher survival ratio was observed in both the pre-con group (78.98 ± 3.39, 62.74 ± 3.7, and 54.42 ± 4.45 %) and the post-con group (77.42 ± 4.14, 59.74 ± 6.67, and 49.52 ± 4.13 %) than the control group (45.22 ± 3.69, 42.44 ± 3.76, and 33.2 ± 3.29 %) at 3, 12, and 18 h postoperatively (P < 0.05). There was no statistical difference between the pre-con group and the post-con group (P > 0.05). Histological examination showed delayed and attenuated tissue damage in both the pre-con group and the post-con group when compared to that of the control group. A higher expression of iNOS was observed in both the pre-con group and the post-con group than the control group and the sham group (P < 0.05).

Conclusions

Significant improvement of flap survival could be achieved by both preconditioning and postconditioning treatments; however, better protection could be provided by preconditioning. The higher expression of iNOS may play an important role in the therapeutic effect of postconditioning during I/R injury.