大鼠鼻咽癌变过程中全血硒和谷胱甘肽过氧化物酶的变化

作者观察了在二亚硝基哌嗪(DNP)诱发大鼠鼻咽癌变过程中全血硒浓度和谷胱甘肽过氧化物酶(GSH-PX)活性的变化及硒的作用。实验分5组:①空白对照组(BG);②硒对照组(SG);③DNP对照组(DG);④硒预防组(PG);⑤硒治疗组(TG)。结果显示,于饮水中补硒能提高大鼠血硒浓度和GSH-PX活性,而且能预防鼻咽癌的发生;在实验早期,各给致癌物组酶活性与相应的对照组无差异,后期前者均明显低于后者,且DG血硒浓度明显低于BG,说明肿瘤的形成引起了血中GSH-Px和硒浓度的降低;然而PG和TG血硒与SG无差异,说明血硒浓度的高低易受摄取量的影响,提示在评估肿瘤时,血GSH-PX活性也许比血硒更有用。经分析,大鼠全血GSH-PX活性与硒浓度之间有正相关关系(r=0.61,P相似文献   

肝炎和肝硬化患者胆囊超声改变的观察与分析

目的 :研究肝炎肝硬化患者胆囊彩色B超声像图变化 ,并探讨其临床意义。方法 :采用彩色超声诊断仪对 139例肝炎和肝硬化患者及 4 2例非肝炎体检者进行胆囊超声检查。结果 :慢性肝炎、重型肝炎、肝硬化患者组与非肝炎对照组彩色B超胆囊异常率比较均差异显著 (P <0 .0 1)。重型肝炎、肝硬化组与慢性肝炎组之间胆囊异常率也存在明显的差异 (P <0 .0 5 )。结论 :慢性肝病患者胆囊异常与胆囊本身炎症无关。胆囊声像图的改变对判断肝脏实质性病变的严重程度及指导临床治疗有一定的参考价值。     

中效人胰岛素联合瑞格列奈治疗磺脲类降糖药继发性失效临床观察

目的观察中效人胰岛素与瑞格列奈联合应用治疗磺脲类降糖药继发性失效的疗效。方法将磺脲类降糖药继发性失效(SFS)36例病例随机分成两组,分别接受睡前小剂量中效人胰岛素加瑞格列奈(A组,20例)和单纯瑞格列奈(B组,16例)治疗,比较两组各生化指标。结果①治疗前空腹血糖(FPG)、餐后2小时血糖(2hPG)、C肽(C-P)、糖化血红蛋白(HbA1c)水平两组间无统计学差异;②治疗3个月后两组自身的FPG、2hPG、C-P、HbA1c水平均较治疗前明显改善,P值分别为0.01、0.05、0.05、0.01;③治疗3个月后FPG、2hPG、C-P、HbA1c水平A组均低于B组,P值分别<0.01、0.05、0.05、0.01。结论中效人胰岛素联合瑞格列奈和单用瑞格列奈治疗磺脲类降糖药继发性失效均有疗效,胰岛素和瑞格列奈联用优于单用瑞格列奈。     

Cloning of Human α-defensin-1 (HNP-1) Gene and Construction of Its Eukaryotic Expression Vector

1 Introduction Defensins are small cationic antimicrobial peptides that function in the host's innate immune system. The human defensin family includes three small peptides from the azurophil granules of polymorphonuclear cells named human neutrophil peptide (HNP)-1, HNP-2, HNP-3,which consist 5%-7% of the protein of human neutrophil. HNP-4 is approximately one hundred times less abundant. They demonstrate antibacterial, antifungal and antiviral properties in vitro. HNPs are important component of nonoxidative mechanism in macrophages, and can direct inactivate the enveloped viruses. Because only special cells express defensins. And it is hard to extract them naturally and the production is few. So researcher expect to obtain defensins highly through heterogenous expression by gene engineering technology. In order to express HNP-1, we cloned the cDNA of HNP-1 from human polymorphonuclear cells in peripheral blood, and constructed its eukaryotic expression vector, which provided a base for the further study on its mechanism of antimicrobial effect.     

移植于脊髓的断离肋间神经重发新支至原去神经肋间肌的实验观察

将15只大鼠左侧第11或12肋间神经从根部向外,自周围组织完全分离出20～35mm,并于此处剪断。再将其近端改向植入脊髓左侧半 L_(1～2) 之间的髓节内。在存活57～413天后解剖观察,发现自移植神经的干上发出1～3条新分支,分布于原去神经的肋间肌。经光、电镜观察证实是成活的再生神经纤维。于移植神经干注射 CB-HRP 后,发现这些神经纤维来源于原肋间神经髓节的前角和移植部髓节内的神经细胞。故认为外周神经缺损,不作神经对接或桥接,不经断离神经的远段,在一定条件下可以由近段发出再生纤维组成新支,分布于去神经的靶组织。     

香萱解郁方对血清剥夺诱导HT22细胞损伤模型的神经保护作用

探讨香萱解郁方含药血清对血清剥夺致小鼠海马神经元HT22细胞损伤模型的影响。方法 采用血清剥夺培养HT22细胞建立神经损伤体外模型,实验分为对照组、模型组和中药组［中药组A（含药血清15%浓度）、中药组B（含药血清20%浓度）］,各组血清剥夺12 h后,通过CCK8法检测各组细胞活力,确定15%浓度含药血清干预后细胞的存活率最高,设定为后续实验中药组的药物浓度。免疫荧光染色法检测神经元特异性微管蛋白（β-Tubulin Ⅲ）在各组中的表达,蛋白质免疫印迹法及qPCR法检测脑源性神经营养因子（BDNF）蛋白及其mRNA在各组中的表达。结果 在加药后培养12 h,中药组的细胞活力明显高于对照组与模型组（P＜0.001）。培养24 h,模型组细胞活力较对照组明显下降（P＜0.001）,中药组较模型组明显提高（P＜0.001）。培养36 h,模型组细胞活力较对照组显著下降（P＜0.001）,中药组较模型组明显提高（P＜0.001）。模型组BDNF蛋白含量及 BDNF mRNA表达量较对照组显著降低（P＜0.05,P＜0.001）,模型组少数神经元长出突起;中药组BDNF蛋白含量及BDNF mRNA表达量较模型组显著增加（P＜0.05）,中药组HT22细胞轴突突起长度和分支增加,β-Tubulin Ⅲ阳性表达明显增多。结论 香萱解郁方含药血清对血清剥夺诱导小鼠海马神经元HT22细胞损伤具有神经保护作用,可能与促进BDNF蛋白表达有关     

The nuclear pore complex protein Tpr is a common autoantigen in sera that demonstrate nuclear envelope staining by indirect immunofluorescence

We studied the autoantigen targets of 75 human sera that had antibodies to the nuclear envelope (NE) as identified by indirect immunofluorescence (IIF) on HEp‐2 cells. Several different IIF staining patterns could be identified when antibodies to different components of the nuclear membrane (NM) and nuclear pore complexes (NuPC) were identified: a smooth membrane pattern characteristic of antibodies to nuclear lamins, a punctate pattern typical of antibodies to the nuclear pore complex and more complex patterns that included antibodies to nuclear and cytoplasmic organelles. Western immunoblotting of isolated nuclear and NE proteins and immunoprecipitation of radiolabelled recombinant proteins prepared by using the full‐length cDNAs of the Translocated promoter region (Tpr), gp210 and p62 were used to identify specific autoantibody targets. Fifty‐two of the 75 (70%) sera bound to Tpr, 25 (33%) bound to lamins A, B or C, 15 (20%) reacted with gp210 and none reacted with p62. Sixteen (21%) did not react with any of the NE components tested in our assays. The clinical features of 37 patients with anti‐NE showed that there were 34 females and three males with an age range of 16–88 years (mean 59 years). The most frequent clinical diagnosis (9/37 = 24%) was autoimmune liver disease (ALD; two with primary biliary cirrhosis), followed by seven (19%) with systemic lupus erythematosus (SLE), four (11%) with a motor and/or sensory neuropathy, three (8%) with anti‐phospholipid syndrome (APS), two with systemic sclerosis (SSc), two with Sjögren's syndrome (SjS), and others with a variety of diagnoses. This report indicates that Tpr, a component of the NuPC, is a common target of human autoantibodies that react with the NE.     

抗人喉癌/抗VEGF双功能抗体制备及应用研究

目的：研制抗人喉癌／抗血管内皮因子（VEGF）双功能克隆抗体，用于喉癌抗血管生成治疗。方法：采用二次杂交瘤技术制备抗人喉癌／抗VEGF双功能抗体。经酶联免疫吸附试验法和SP法检测喉癌及癌前病患者血清及癌组织中VEGF的含量表达。结果：获得6株分泌抗人喉癌／抗VEGF双功能抗体的杂交瘤，经免疫组化证实与喉癌细胞特异性结合率为93％，而与血管内皮细胞结合率为89％。血清中VEGF含量表达，喉癌组与癌前病组及正常对照组相比差异均显著。IgG亚型鉴定为IgG2aBSAb抗体效价为1：25 600倍（ELISA法）。结论：二次杂交瘤法制备的双功能抗体具有均匀性、可控性、效价高、稳定性好，可用于喉癌抗血管生成治疗，动态检测可作为判断喉癌预后的客观指标。     

中国人的婚姻质量状况

目的：调查中国人婚烟满意状况。方法：用中国人婚姻质量问卷对1303名已婚者进行调查，其中夫妻配对资料224对。结果：无论是男性还是女性，总体满意度都处于中等水平，基本上呈正态分布，仅在性格相容和子女婚姻两个维度有统计学差异；约60％以上的人觉得自己的婚姻比较满意，30％的人认为基本满意，5％的体验到非常满意，只有2％左右的人感到不满意；夫妻婚姻质量具有较高的相关性，主观满意度一致的人数占60％以上，不一致或很不一致的人数只占100％。结论：中国人的总体婚姻质量处于中等水平，90％的夫妻对自己的婚姻感到满意，只有2％左右的人感到不满意。