利多卡因对缺氧/复氧后肝细胞Bcl-2、Caspase-3蛋白表达及细胞凋亡的影响

目的探讨利多卡因预处理对肝细胞缺氧/复氧后Bcl-2、Caspase-3蛋白表达及细胞凋亡的影响。方法将体外培养的肝细胞分为三组:缺氧/复氧组(Ⅰ组)、利多卡因预处理组(Ⅱ组)和正常对照组(Ⅲ组),每组10份。检测肝细胞缺氧/复氧培养后细胞培养液中谷丙转氨酶(ALT)、谷草转氨酶(AST)浓度,肝细胞Bcl-2、Caspase-3蛋白表达、肝细胞凋亡率及超微结构变化。结果Ⅰ、Ⅱ组细胞培养液中ALT、AST浓度、肝细胞Caspase-3蛋白表达和肝细胞凋亡率较Ⅲ组均升高(P<0.05),透射电镜示肝细胞损伤,可见凋亡细胞;Ⅱ组细胞复氧后培养液中ALT、AST浓度、肝细胞Caspase-3蛋白表达和细胞凋亡率均低于Ⅰ组(P<0.05),而肝细胞Bcl-2蛋白表达较Ⅰ组升高(P<0.05),透射电镜观察也显示Ⅱ组肝细胞比Ⅰ组损伤轻微。结论利多卡因预处理可以在一定程度上减轻缺氧/复氧后肝细胞损伤,降低细胞凋亡率,保护机制可能与Bcl-2、Caspase-3蛋白表达有关。     

Effect of l-carnitine supplementation on renal anemia in patients on hemodialysis: a meta-analysis

International Urology and Nephrology - l-carnitine is an amino acid derivative that is thought to be helpful for treating renal anemia in hemodialysis patients. However, the mechanism remains to be...     

Alternate methods for laparoscopic management of adnexal masses greater than 10 cm in diameter.

PURPOSE: We describe alternate laparoscopic methods for inspection and removal of large adnexal masses, and report our experience with 18 cases in which these methods were used. PATIENTS AND METHODS: Between April 1994 and January 2000, the first author performed operative laparoscopy on 18 patients, each of whom had at least one adnexal mass with maximum diameter greater than 10 cm. Mean patient age was 32 years (range 11 to 82). Seventeen of the 18 patients were premenopausal. All procedures were performed at one of two community hospitals in Seattle or at Yuan's General Hospital in Taiwan. Preoperative screening included pelvic exam, tumor markers, and ultrasound. RESULTS: One 82-year-old patient underwent planned laparoscopic bilateral oopherectomy. In the other 17 cases the operative goal was cystectomy or unilateral oopherectomy with conservation of reproductive function. Cystectomy was successfully performed in five of these cases (29.4%). The remaining 12 patients underwent either unilateral oopherectomy (10 cases, 58.8%), or unilateral salpingo oopherectomy (2 cases, 8.8%) due to the extent of their mass. Sixteen of the 18 cases in this series were successfully managed by a single laparoscopic surgery, one case required a second-look laparoscopy, and in one case a malignancy was found by histological analysis of permanent section, which required a second laparoscopy for staging and debulking. CONCLUSIONS: Large adnexal masses can be successfully managed with minimal hospital stay using laparoscopic techniques, when care is taken to avoid rupture and spillage of cyst contents, and thorough inspection of the mass and abdominal cavity is made possible. The probability of finding an unexpected malignancy is low. In those cases where a malignancy is found, appropriate cytoreductive staging surgery can be performed immediately.     

Effects of Scutellaria Baicalensis on Activity and Biofilm Formation of Klebsiella Pneumoniae

Objective To explore the effects of Scutellaria baicalensis on activity and biofilm formation of Klebsiella pneumonia (Kp). Methods The broth and agar dilution methods were carried out to determine minimum inhibitory concentration and minimum bactericidal concentration of Scutellaria baicalensis for TW518. VITEK-32 system was used to assay TW518 susceptibility to antibiotics. Kp biofilms were formed in vitroand stained with BacLight Live/Dead stain. The class integron geneⅠ1 mRNA expression was analyzed with RT-PCR. Results The minimum inhibitory concentration of Scutellaria baicalensis on TW518 identified as a Kp colony was 32 mg/ml, and minimum bactericidal concentration was 64 mg/ml. Scutellaria baicalensis and broad-spectrum penicillin, cephalosporin, quinolones, or beta-lactamase had synergistic bactericidal effects. Biofilm formation activity of Kp treated with Scutellaria baicalensis was significantly lower than that of the control group. And class integron geneⅠ1 mRNA expression of TW518 was significantly inhibited by Scutellaria baicalensis. Conclusions Scutellaria baicalensis has sterilization effect on Kp, and Scutellaria baicalensis could effectively inhibit Kp biofilm formation with prolonged treatment. Scutellaria baicalensis might inhibit Kp biofilm formation through down-regulating integron geneⅠ1 expression.     

二甲双胍抑制骨肉瘤MG63细胞的迁移和侵袭能力

目的 观察二甲双胍对骨肉瘤MG63细胞的迁移侵袭能力的影响.方法 以骨肉瘤MG63细胞株为研究对象,二甲双胍处理后分别采用transwell小室法检测细胞迁移和侵袭能力的变化,采用明胶酶谱实验检测基质金属蛋白酶2(MMP-2)和MMP-9的活性,采用real-time PCR法检测细胞中埃兹蛋白(Ezrin) mRNA的表达,采用Western blot检测Ezrin蛋白的表达;Li-pofectamine 2000转染Ezrin质粒到细胞中观察其对二甲双胍诱导的细胞迁移侵袭抑制及对下游信号通路的影响.结果 二甲双胍可显著抑制MG63细胞的迁移和侵袭,并降低MMP-2和MMP-9的酶活性.用药48 h后,MG63细胞内Ezrin的mRNA和蛋白水平均明显下降,且上调Ezrin可减弱二甲双胍诱导的骨肉瘤细胞迁移和侵袭抑制及二甲双胍诱导的MAPK/Erk信号通路抑制.结论 二甲双胍可抑制MG63细胞的迁移和侵袭能力,可能是通过下调Ezrin和MAPK/Erk信号通路而实现.     

基于SWOT分析的医学网络在线考核方法的策略

运用SWOT法对新形势下医学网络在线考核从优势（strength）、劣势（weakness）、机会（opportunity）和威胁（threat）4个维度展开探讨,并把各因素相互匹配加以分析,提出SO、WO、ST和WT四类战略,以期能为医学考核方式未来的发展提供思路与建议。     

振幅整合脑电图联合P50 对重症脑血管病 患者预后结局的评估

目的 研究振幅整合脑电图联合感觉门控电位P50 对重症脑血管病患者预后结局的评估价值。 方法 选取2017 年5 月—2019 年5 月郴州市第一人民医院诊治的重症脑血管病患者62 例。根据患者预后情 况将其分成病死组（14 例）和预后良好组（48 例）,比较两组患者振幅整合脑电图、P50 结果;分析振幅整 合脑电图、P50 与格拉斯哥预后评分表（GOS）评分的相关性;评估振幅整合脑电图、P50 单独与联合检测 对患者的预后价值。结果 两组振幅整合脑电图分级比较,差异有统计学意义（P <0.05）。病死组P50 较预 后良好组高（P <0.05）。预后良好组GOS 评分较病死组高（P <0.05）。振幅整合脑电图、P50 与GOS 评分呈 负相关（r s =-0.629 和-0.703,均P <0.05）。联合检测对患者预后的价值高于振幅整合脑电图、P50单独检测 （P <0.05）。结论 P50 联合振幅整合脑电图检测对重症脑血管病患者具有较高的预后评估价值。     

利用果蝇原代细胞系统研究Gef26对肌肉细胞F-actin的调控机制

目的：探讨Gef26对果蝇原代细胞发育的影响。方法：通过培养野生型、gef26突变体以及gef266/+;abl4/+双突变体果蝇胚胎的原代细胞,观察细胞形态。对细胞骨架F-actin进行荧光染色,观察细胞骨架的分布。结果：在果蝇胚胎的原代细胞中,野生型肌肉细胞形态正常,gef26突变体的肌肉细胞形态明显异常;gef266/+;abl4/+双突变体肌肉细胞形态正常,即阿贝尔森酪氨酸激酶（Abelson tyrosine kinase, Abl）突变挽救gef26突变体肌肉细胞形态异常的表型;野生型肌肉细胞中的F-actin呈现出规整的形态和连续的排布,而两株gef26突变体肌肉细胞中的F-actin排布却呈现出不规整的形态和不连续的排布; gef266/+;abl4/+双突变体细胞中的F-actin呈现出规整的形态和连续的排布,即Abl突变挽救gef26突变体F-actin组装紊乱的表型。结论：Gef26影响肌肉细胞的细胞骨架组装,实验结果提示Gef26通过Abl信号通路调控肌动蛋白的细胞骨架组装。     

香萱解郁方对血清剥夺诱导HT22细胞损伤模型的神经保护作用

探讨香萱解郁方含药血清对血清剥夺致小鼠海马神经元HT22细胞损伤模型的影响。方法 采用血清剥夺培养HT22细胞建立神经损伤体外模型,实验分为对照组、模型组和中药组［中药组A（含药血清15%浓度）、中药组B（含药血清20%浓度）］,各组血清剥夺12 h后,通过CCK8法检测各组细胞活力,确定15%浓度含药血清干预后细胞的存活率最高,设定为后续实验中药组的药物浓度。免疫荧光染色法检测神经元特异性微管蛋白（β-Tubulin Ⅲ）在各组中的表达,蛋白质免疫印迹法及qPCR法检测脑源性神经营养因子（BDNF）蛋白及其mRNA在各组中的表达。结果 在加药后培养12 h,中药组的细胞活力明显高于对照组与模型组（P＜0.001）。培养24 h,模型组细胞活力较对照组明显下降（P＜0.001）,中药组较模型组明显提高（P＜0.001）。培养36 h,模型组细胞活力较对照组显著下降（P＜0.001）,中药组较模型组明显提高（P＜0.001）。模型组BDNF蛋白含量及 BDNF mRNA表达量较对照组显著降低（P＜0.05,P＜0.001）,模型组少数神经元长出突起;中药组BDNF蛋白含量及BDNF mRNA表达量较模型组显著增加（P＜0.05）,中药组HT22细胞轴突突起长度和分支增加,β-Tubulin Ⅲ阳性表达明显增多。结论 香萱解郁方含药血清对血清剥夺诱导小鼠海马神经元HT22细胞损伤具有神经保护作用,可能与促进BDNF蛋白表达有关