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11.
Kamagata C Tsuji N Moriai M Kobayashi D Watanabe N 《Breast cancer research and treatment》2007,102(3):263-273
The cyclopentenone prostaglandin 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) exerts a growth inhibitory effect on cancer cells, and this effect is linked to the induction of apoptosis or cell cycle
arrest. Induction of apoptosis by 15d-PGJ2 is associated with the down-regulation of anti-apoptotic proteins. G0-G1→S phase progression is inhibited by 15d-PGJ2 via the degradation of cyclin D1. In this study, we further investigated the mechanism by which 15d-PGJ2 inhibits cancer cell growth by using the breast cancer cell lines MCF-7 and T-47D. Treatment with 20 μM 15d-PGJ2 for 72 h completely blocked the growth in both cell lines. However, the proportions of apoptotic MCF-7 and T-47D cells were
21.1% and 40.9%, respectively, indicating that the induction of apoptosis did not appear to fully account for growth inhibition
by 15d-PGJ2. Cell cycle analysis using cells synchronized at the G0-G1 or S phase revealed that 15d-PGJ2 blocked not only G0-G1→S phase progression but also G2-M phase progression. The expression of both cyclins D1 and B1 was decreased by 15d-PGJ2. Furthermore, 15d-PGJ2 inhibited aurora-B kinase activity, which coincided with the down-regulation of survivin. Thus, 15d-PGJ2 induced cell cycle arrest at the G2-M phase via inhibition of cyclin B1 expression and aurora-B kinase activity. We conclude that survivin may be an important
target for 15d-PGJ2, and its down-regulation may lead to a decrease in aurora-B kinase activity.
Naoki Tsuji substantially contributed to this work and should also be considered a first author. 相似文献
12.
Shoichi Ishiura Nika Hotoda Beáta Szabó Masashi Asai Chinatsu Hattori Eiichiro Tanaka Hisashi Koike Noboru Sasagawa 《Psychogeriatrics》2001,1(4):273-276
Abstract: Multiple lines of evidence suggest that increased production and deposition of amyloid β peptide (Aβ) contribute to Alzheimer's disease (amyloid hypothesis). Several factors regulate processing of amyloid precursor protein. Mechanisms of Aβ production and clearance from the brain have been the subject of considerable interest. Here we summarize recent advances in the identification of amyloid secretases and rational drug design. 相似文献
13.
Kanzaki M Yamato M Yang J Sekine H Kohno C Takagi R Hatakeyama H Isaka T Okano T Onuki T 《Biomaterials》2007,28(29):4294-4302
Current methods including the use of various biological and synthetic sealants are ineffective in the closure of intraoperative air leaks that often occur during cardiothoracic surgeries, resulting in a decreased quality of life for patients. We present the development of a novel lung air leak sealant using tissue engineered cell sheets. In contrast to previous materials such as fibrin glue, these bioengineered cell sheets immediately and permanently seal air leaks in a dynamic fashion that allows for the extensive tissue contraction and expansion involved in respiration, without any postoperative recurrences. Additionally, we demonstrate that mesothelial cells migrate to cover the transplanted cells sheets, thereby confirming excellent biocompatibility and integration with the host tissues. Finally, we present the use of skin fibroblasts as an effective and readily available autologous cell source that can be easily applied. This study shows for the first time, the development of an immediate and permanent lung air leak sealant, suitable for future clinical applications. 相似文献
14.
Iida C Fujii K Koga E Washino Y Ichi I Kojo S 《Journal of nutritional science and vitaminology》2007,53(2):160-165
D-Galactosamine (D-Galn: 300 mg/kg) was intraperitoneally administered to rats. After 6 h the activity of plasma GOT and GPT was significantly higher than that of the control group and plasma GOT and GPT activities increased thereafter. These results indicated that the necrotic process was initiated at about 6 h and developed thereafter. With coadministration of DMSO (1 h before administration of D-Galn: 2.5 mL/kg, oral), plasma GOT and GPT were significantly lower, showing that DMSO inhibited the necrotic action of D-Galn. After 6-24 h of D-Galn administration, the hepatic level of vitamin C, the most sensitive indicator of oxidative stress, decreased significantly, indicating that oxidative stress was significantly enhanced 6 h after D-Galn intoxication and thereafter. DMSO significantly restored the liver vitamin C level 24 h after D-Galn injection, demonstrating that DMSO effectively ameliorated the oxidative stress caused by D-Galn, resulting in the prevention of necrosis of the liver. Phosphorylated JNK and phospho-ERK were significantly increased transiently 6-12 h after treatment with D-Galn. These results indicated that oxidative stress and the activation of JNK took place almost simultaneously. Phosphorylated p38 MAPK was not changed and DMSO treatment did not affect the change of these MAPKs by D-Galn. 相似文献
15.
Takaaki Ogoshi Takashi Kido Kazuhiro Yatera Keishi Oda Chinatsu Nishida Kei Yamasaki Takeshi Orihashi Yukiko Kawanami Hiroshi Ishimoto Masashi Taguchi Masaru Harada Hiroshi Mukae 《Respirology (Carlton, Vic.)》2015,20(7):1142-1144
We evaluated the incidence and outcome of lung involvement in 35 patients with autoimmune pancreatitis (AIP). Our results indicate that lung involvement is commonly observed in AIP (40%). In addition, corticosteroid treatment improved the lung lesions and appeared to reduce the probability of relapse compared with pancreatic lesions (0% vs 36%). This is the first report to assess the long‐term outcome of lung involvement in AIP (52 ± 33 months). 相似文献
16.
Kozue Akamine Keiko Mekaru Keiya Gibo Chinatsu Nagata Rie Nakamura Sugiko Oishi Maho Miyagi Chiaki Heshiki Yoichi Aoki 《Reproductive Medicine and Biology》2021,20(1):76-82
PurposeTo investigate impact of the one‐carbon metabolism (OCM) on oocyte maturity and embryo development.MethodsThis prospective study analyzed 18 women who agreed to participate. We measured the OCM biomarkers’ concentrations including Vitamin B12 (VB12), folic acid (FA), and homocysteine (Hcy) in serum and follicular fluid (FF), and assessed their correlation. We also evaluated the influence of such OCM biomarker concentrations in mono‐FF on oocyte maturation, fertilization, embryo quality, and consequent pregnancy after embryo transfers.ResultsAll biomarkers showed a high concentration variability in different follicles of each woman, but their mean levels correlated with the serum levels. Among the 106 collected oocytes, 92 were mature, 59 were fertilized, and 16 yielded good‐quality embryos. We performed 26 single embryo transfers, and 7 patients achieved clinical pregnancies. VB12 concentration (FF) was significantly lower in fertilized than unfertilized oocytes by univariate analysis. In multivariate logistic analysis, a significant correlation was found between FA concentration (FF) <14.25 ng/mL and good‐quality embryos and between Hcy concentration (FF) <4.9 nmol/mL and clinical pregnancy.ConclusionOCM in FF may affect fertilization, embryo quality, and clinical pregnancy. 相似文献
17.
Saito I Ozawa H Bello MC Moriwaki C Ito M Aono H Ikebe T Miyata A Itoga T 《Environmental health and preventive medicine》1998,3(1):31-36
Coronary heart disease (CHD) is the leading cause of death according to official mortality statistics in the Dominican Republic.
The purpose of this study was to assess food intake and food consumption patterns of Dominicans and to discuss CHD risks related
to nutrient intake. The subjects were 22 men and 81 women voluntarily recruited among 230 hospital workers in Santo Domingo.
The mean nutrient intake and food consumption were estimated from a single 24-hour recall method administered by trained persons
who elicited each subject’s previous day’s intake from midnight to midnight. The mean energy intake was 2030 kcal. The mean
intake of total protein, total fat and carbohydrates were 66.3g, 89.1 g and 248g, respectively. Percentage of energy from
total fat was 39.5%, which was higher than WHO recommendations. But, the percentage of animal fat per total fat was 46.8%.
Especially, the value in the low income group was 31.7%. Moreover, we analyzed the food consumption structure in order to
understand the Dominican dietary pattern, and three factors were found to explain the characteristics on the basis of factor
analysis.
The low percentage of animal fat was due to the diets rich in vegetable oil. Our results suggested that it was difficult to
assume that CHD risks increased as a result of the Dominican dietary pattern. This was consistent with our previous findings
that the average serum cholesterol level of Dominicans was generally low. 相似文献
18.
Inoue Y Trapnell BC Tazawa R Arai T Takada T Hizawa N Kasahara Y Tatsumi K Hojo M Ichiwata T Tanaka N Yamaguchi E Eda R Oishi K Tsuchihashi Y Kaneko C Nukiwa T Sakatani M Krischer JP Nakata K;Japanese Center of the Rare Lung Diseases Consortium 《American journal of respiratory and critical care medicine》2008,177(7):752-762
19.
20.
Abe M Suzuki K Sakata C Sugasawa K Hirayama F Koga Y Kawasaki T Naganuma S Itoh H 《European journal of pharmacology》2011,(1):58-63
Eltrombopag, an orally-active small molecule thrombopoietin (TPO) receptor agonist, was used for the first time in 2008 to treat patients with chronic idiopathic thrombocytopenic purpura. Here, we investigated the pharmacological effect of a new orally-active small molecule TPO receptor agonist which may be effective in treating these patients. 50% effective concentration values for cell proliferation with AS1670542 or eltrombopag were 1.9 and 13 nM, respectively, while those for megakaryocyte colony formation from human cord blood CD34+ cells with AS1670542 or eltrombopag were 260 and 950 nM, respectively. On Day 14 after the start of administration, AS1670542 significantly increased the number of human platelets in non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice with transplanted human hematopoietic stem cells at 0.3 (P < 0.05); in contrast, while administration of eltrombopag also increased the numbers of these platelets at 30 mg/kg/day (P = 0.058), no statistical significance was noted in the increase. Here, we identified AS1670542, a novel orally-active TPO receptor agonist which mimics the biological activity of TPO and may demonstrate greater in vitro and in vivo pharmacologically efficacy than eltrombopag. 相似文献