Regional hyperthermia combined with high energy shock wave treatment on Dunning prostate tumors

Male Copenhagen rats implanted with Dunning R3327 prostate carcinoma were treated with regional hyperthermia and high energy shock waves (HESW). Twenty-four rats were divided into four equal groups. All rats in one group were matched with the corresponding rats in the other three groups according to tumor size. Hyperthermia was created by the resistive heating of a 22-gauge 1.5-cm needle; HESW were generated by the Dornier XL-1 experimental lithotripter. In two hyperthermia treatments administered 48 hours apart, the tumor was heated to 46.5 degrees C for 2 hours; HESW treatment involved 1,600 shocks at 18 kV. Twenty-nine days after initiation of this study, animals were sacrificed and tumor growth calculated. Tumor growth in group 1 (combined hyperthermia and HESW) was significantly delayed between the 7th and 29th day (p = 0.05). In group 2 (HESW alone), tumor growth was significantly delayed between the 7th and 22nd day (p = 0.01), while tumor growth in group 3 (hyperthermia alone) was delayed between the 3rd and 22nd day (p = 0.02). On day 22, mean percent change in tumor growth in the control group exceeded that of group 1 by 449% (p = 0.015) as well as that of groups 2 and 3, by 350% (p = 0.034) and by 268% (p = 0.049), respectively. As previous reports demonstrate the efficacy of hyperthermia in combination with irradiation or chemotherapy for the treatment of prostate carcinoma, our results indicate hyperthermia in combination with HESW may also be a useful treatment modality.     

哇巴因与地高辛对大鼠肝脏钠泵A亚单位基因表达的影响

目的　观察哇巴因与地高辛对大鼠肝脏钠泵基因表达的影响 .方法　分别给大鼠注射哇巴因 (2 0 μg· kg- 1·d- 1 )、地高辛 (32 μg·kg- 1·d- 1 )和生理盐水 (1m L· kg- 1·d- 1 ) ,观察给药后 6wk大鼠血压的动态变化 ;并分别应用分子生物学 RT- PCR及免疫组织化学技术 ,探讨各组大鼠肝脏钠泵 α1 ,α2 及 α3 亚单位 m RNA及蛋白水平基因表达的改变 .结果　给予哇巴因 2 wk后大鼠血压开始升高 ,至 6 wk时明显高于生理盐水组 (17.7± 1.2 ) vs(15 .4± 1.1) k Pa,P<0 .0 1) ,而实验过程中地高辛组血压与对照组比较差异不明显 .无论是在 m RNA水平还是在蛋白水平 ,哇巴因对大鼠肝脏钠泵α1 亚单位表达无影响 ,而地高辛使α1 亚单位表达增强 ;两组大鼠 α2 亚单位表达均无改变 ;哇巴因使 α3 亚单位蛋白水平表达减弱 ,而 m RNA水平增强 ,地高辛组大鼠肝脏钠泵 α3 亚单位无论在 m RNA水平及蛋白水平表达均增强 .结论　哇巴因在高血压发病中可能起着重要作用 ;哇巴因与地高辛可导致不同的钠泵基因表达改变 ,为进一步揭示内源性哇巴因生理与病理作用以及洋地黄类药物药理与毒理作用的分子机制提供了理论及实验依据     

小鼠补体受体Ⅱ型基因改造后感染EB病毒

通过对小鼠补体受体Ⅱ型基因（CR2）进行定点突变,然后将野生型和突变型小鼠CR2/1（MCR2/1）及人CR2（hCR2)用电穿孔方法导入小鼠SP2/0进行表达,采用免疫组织化学等方法鉴定阳性细胞系。用EB病毒对转染阳性细胞进行感染,EBER-1杂交结果显示,仅转染hCR2和突变型MCR2（mtMCR2)的SP2/0细胞感染了EB病毒,前者感染EB病毒的阳性率较后者高很多。这为进一步研究EB病毒进入细胞的机制及建立EB病毒相关的鼻咽癌动物模型奠定了良好的基础。     

高位复杂性肛瘘的解剖学切除术

目的 探索治疗高位复杂性肛瘘较理想的手术方式。方法 应用解剖学原理,行瘘管切除、保留括约肌、内口缝合、创面开放术式,对高位复杂性肛瘘进行手术治疗。结果 45例一期治愈,1例二期治愈,无肛门失禁、肛门狭窄等并发症。结论 解剖学切除术是一种治疗高位复杂性肛瘘较理想的手术方式。     

直视下行大鼠气管插管的改良方法

目的 提高大鼠气管插管的成功率;方法 对比传统闭式法与改良直视法的成功率;结果传统闭式法的成功率为50％,改良直视法的成功率为100％。结论 改良直视法优于传统闭式法,值得推广应用。     

鼻咽癌组织中微血管的分布

目的：观察鼻咽癌组织中微血管的分布及存在状况,探讨其与鼻咽癌的临床病理联系。方法：采用免疫组织化学Ｓ－Ｐ法,检测血栓调节蛋白在鼻咽癌血管内皮细胞的表达,进行微血管的定性半定量研究。结果：鼻咽癌组织中微血管的分布不均匀。极性消失。血管呈分支芽状弥漫分布于癌组织中,或呈条索状围绕癌细胞团。微血管的数目与癌细胞的增殖指数屯预后正相关。结论：鼻咽癌组织中微血管数与患者的生存期有关。     

Partial Sequence Analysis of the Genome of Human Herpesvirus 7 YY5 Isolated from Saliva Samples

Ｈｕｍａｎｈｅｒｐｅｓｖｉｒｕｓ 7(ＨＨＶ 7)ｉｓｏｎｅｏｆｔｈｅｍｏｓｔｒｅｃｅｎｔｌｙｄｉｓｃｏｖｅｒｅｄｍｅｍｂｅｒｏｆｔｈｅｈｅｒ ｐｅｓｖｉｒｕｓｆａｍｉｌｙ .Ｉｔｗａｓｆｉｒｓｔｉｓｏｌａｔｅｄｆｒｏｍａｃｔｉｖａｔ ｅｄＣＤ4 Ｔｃｅｌｌｓｆｒｏｍｐｅｒｉｐｈｅｒａｌｂｌｏｏｄｍｏｎｏｎｕ ｃｌｅａｒｃｅｌｌｓ (ＰＢＭＣｓ)ｏｆａｈｅａｌｔｈｙｉｎｄｉｖｉｄｕａｌ[1] ,ａｐａｔｉｅｎｔｗｉｔｈｃｈｒｏｎｉｃｆａｔｉｇｕｅｓｙｎｄｒｏｍｅ(ＣＦＳ) [2 ] ,ａｎｄｆｒｏｍｓａｌｉｖａｏｆｈｅａｌ…     

Reconstruction of the two-dimensional distribution of p53 positive staining patches in sun-exposed morphologically normal skin

1,320 serial sections were microscopically examined from 11 excisions of human skin with a variety of neoplastic lesions. In total about 1 cm(2) of morphologically normal epidermis was scrutinized. The emphasis was on the topography of epidermal 'p53 patches' of morphologically normal keratinocytes apparently driven into clonal expansion by a mutation of the p53 gene or an analogous mechanism causing constitutive nuclear accumulation of p53. The p53 patches were only rarely in contact with dysplasia or carcinoma in situ supporting previous somatic genetic evidence that patches are not common precursors of squamous cell neoplasia. The size of the p53 patches varied from 0.03 to at least 12 mm(2). About 34% of epidermis was covered by p53 patches deduced to be originally derived from an average of 40 cells per cm(2) by clonal expansion. This implies that a large number of skin cells are at risk of contracting further mutations in the p53 gene and other parts of the genome, if the 'guardian of the genome hypothesis' of the p53 gene is correct and exposure to sun-light continues. This finding together with calculations based on number of basal cells/mm(2) skin area suggest that prevalence of mutations which apparently drive stem cells into clonal growth is about 1:15,000 stem cells in 70-80 years old Caucasian sun-exposed skin at latitude 60 degrees North.     

The use of the STRs HUMTH01, HUMVWA31/A,HUMF13A1, HUMFES/FPS,HUMLPL in forensic application: Validation studies and population data for Galicia (NW Spain)

The 5 tetranucleotide short tandem repeats, HUMTHOI, HUMVWA31/A, HUMF13A1, HUMFES/FPS and HUMLPL were studied using different electrophoretic methods and PCR amplification conditions in order to optimize the typing conditions. A genetic population study in the population of Galicia was carried out and the allele and genotype frequencies are given. Compliance with the Hardy-Weinberg equilibrium was tested using different statistical parameters, with clear advantages resulting in favor of using the exact test (Guo-Thompson method) instead of conventional chi-square methods. Some statistical parameters of forensic interest (PD, CE, h) were also calculated. There were no mutations found in a total of 73 paternal meioses and 101 maternal meioses. Abnormal electrophoretic mobility was found in the AT-rich STR HUMF13AI under non-denaturing conditions and, therefore, the use of denaturing conditions is absolutely necessary. No "stutter" bands were found, although double peaks in the HUMFES/FPS system were observed in some samples. The advantage of using automated sequencers with fluorescent technology is also reported.     

大鼠肝细胞线粒体三维结构的观察与重建

为深入研究大鼠肝细胞线粒体形态特征,本研究以钻石刀连续超薄切片,通过电子显微观察大鼠肝细胞线粒体的三维结构,对特殊形态的线粒体进行三维结构重组。结果表明：球形,椭球形,盘状及杆状等小型线粒体约占线粒体总数的５３．８％,而分枝形及各种不规则形的线粒体约占４１．６％。