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排序方式: 共有359条查询结果,搜索用时 15 毫秒
71.
Daniel Pella Stefan Toth Jan Paralic Jozef Gonsorcik Jan Fedacko Peter Jarcuska Dominik Pella Zuzana Pella Frantisek Sabol Monika Jankajova Gabriel Valocik Alina Putrya Andrea Kirschov Lukas Plachy Miroslava Rabajdova Mikulas Hunavy Bibiana Kafkova Ivan Doci Silvia Timkova Mariana Dvorokov Frantisek Babic Peter Butka Lucia Dimunova Maria Marekova Zuzana Paralicova Jaroslav Majernik Jan Luczy Jakub Janosik Martin Kmec 《Archives of Medical Science》2022,18(4):991
IntroductionCurrently, just a few major parameters are used for cardiovascular (CV) risk quantification to identify many of the high-risk subjects; however, they leave a lot of them with an underestimated level of CV risk which does not reflect the reality.Material and methodsThe submitted study design of the Kosice Selective Coronarography Multiple Risk (KSC MR) Study will use computer analysis of coronary angiography results of admitted patients along with broad patients’ characteristics based on questionnaires, physical findings, laboratory and many other examinations.ResultsObtained data will undergo machine learning protocols with the aim of developing algorithms which will include all available parameters and accurately calculate the probability of coronary artery disease.ConclusionsThe KSC MR study results, if positive, could establisha base for development of proper software for revealing high-risk patients, as well as patients with suggested positive coronary angiography findings, based on the principles of personalised medicine. 相似文献
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76.
Francesca Ristalli Salvatore Mario Romano Miroslava Stolcova Francesco Meucci Giovanni Squillantini Serafina Valente Carlo Di Mario 《Cardiovascular Revascularization Medicine》2019,20(4):332-337
Background
Residual aortic regurgitation (AR) complicates a not negligible number of transcatheter aortic valve replacement (TAVR) procedures, and its entity is not always clear at intraprocedural angiographic and echocardiographic control. We applied a minimally invasive hemodynamic monitoring system (Pressure Recording Analytical Method, PRAM) in the setting of TAVR, with the aim of identifying parameters that may help in detection and quantification of residual AR.Methods
We performed hemodynamic monitoring with PRAM in 43 patients undergoing trans-femoral TAVR. Investigated parameters were systolic (Psys, mm?Hg), diastolic (Pdia, mm?Hg), mean (MAP, mm?Hg) and dicrotic pressure (Pdic, mm?Hg), cardiac output (CO, L/min), stroke volume (SV, mL), cardiac cycle efficiency (CCE, Units), dP/dtmax_rad (mm?Hg/ms), MAP-Pdic (mm?Hg).Results
Procedural success was achieved in 86% of the patients; vascular complications occurred in 3 (6.9%), death in 2 (4.7%). Twenty (46.5%) patients had at least mild residual AR. CO, SV, CCE and dP/dtmax_rad changed significantly (p?<?0.001) between baseline and end of procedure in the overall population, with more evident modifications in the subgroup without residual AR. MAP-Pdic variations were statistically significant only in the subgroup without AR (p?=?0.05).Conclusions
TAVR determined an improvement in hemodynamic parameters such as CO, SV, CCE, dP/dtmax_rad. MAP-Pdic was able to discriminate patients with significant residual AR. Hemodynamic monitoring with PRAM system during TAVR is easy and fast to obtain and may help in clinical decision-making in controversial cases. 相似文献77.
Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs 总被引:1,自引:0,他引:1
Rovenská M Hlouchová K Sácha P Mlcochová P Horák V Zámecník J Barinka C Konvalinka J 《The Prostate》2008,68(2):171-182
BACKGROUND: Prostate specific membrane antigen (PSMA), also called glutamate carboxypeptidase II (GCPII), is a target enzyme for diagnosis and treatment of prostate cancer. Moreover, it is upregulated in the vasculature of most solid tumors and is therefore a potential target for the generation of novel antineoplastics. In this context, we analyze the possibility of using rat and pig as animal models for enzymologic and in vivo studies. METHODS: We prepared the recombinant extracellular part of human, rat, and pig GCPII in S2 cell media and characterized the activity and inhibition profiles of the three orthologs by radioenzymatic assay. We performed Western blot analysis of GCPII expression in human, rat, and pig tissues using the monoclonal antibody GCP-04 and confirmed these findings by activity measurements and immunohistochemistry. RESULTS: The three recombinant proteins show similar specific enzymatic activities and inhibition profiles. Tissue expression analysis revealed that most of the pig and human tissues show at least some GCPII-positivity, while the expression pattern in rat is more restricted. Moreover, tissues such as prostate and testes exhibit different GCPII expression levels among the species studied. CONCLUSIONS: The rat and pig orthologs of GCPII seem to be suitable to approximate human GCPII in enzymologic studies. However, the diffuse expression pattern of GCPII in animal and human tissues could be a caveat for the potential utilization of GCPII-targeted anticancer drugs. Furthermore, variations in GCPII tissue distribution among the species studied should be considered when using rat or pig as models for antineoplastic drug discovery. 相似文献
78.
An ethnobotanical study on the usage of wild medicinal herbs from Kopaonik Mountain (Central Serbia) 总被引:2,自引:0,他引:2
Jarić S Popović Z Macukanović-Jocić M Djurdjević L Mijatović M Karadzić B Mitrović M Pavlović P 《Journal of ethnopharmacology》2007,111(1):160-175
An ethnobotanical survey was carried out on the territory of the highest mountain in Central Serbia, Kopaonik, which is characterized by great plant diversity. In total, 83 wild species from 41 families and 96 preparations for use in human therapy were recorded. Among those wild plants which are most commonly used for medicinal purposes, Hypericum perforatum L., Urtica dioica L., Achillea millefolium L., Matricaria chamomilla L., Sambucus nigra L., and Thymus serpyllum L. were particularly highly recommended by the majority of informants as being 'beneficial for all ailments'. The most frequently reported medicinal uses were for treating gastrointestinal ailments (50%), skin injuries and problems (25.6%), followed by respiratory, urinary-genital and cardiovascular problems (20.5%, 20.5%, 19.2%, respectively). Plants with unusual phytotherapeutic uses are Galium verum L. (sedative properties) and Eupatorium cannabinum L. (influenza-like illnesses), while plants with interesting but lesser-known properties include Daphne laureola L. (rheumatism and skin ailments) and Ficaria verna Huds. (tubers for treating haemorrhoids). In addition, 10 wild species used in veterinary medicine, as well as 25 herbs used for human nourishment were noted. 相似文献
79.
Dejan Gođevac Vele Tešević Vlatka Vajs Slobodan Milosavljević Miroslava Stanković 《Food and chemical toxicology》2009
This study addresses in vitro effects of raspberry (Rubus idaeus) seed extracts (RSE) on the frequency of micronuclei. We evaluated the effects of three different extracts (50%, 80%, and 100% methanol) in doses of 1.4, 4.2, and 8.4 μg/mL, per 5 mL culture using cytochalasin-B micronucleus (CBMN) assay in peripheral human lymphocytes. The frequency of MN was scored in binucleated (BN) cells. The nuclear proliferation index was also calculated. The distribution of polyphenolic compounds in RSEs was determined using LC/UV/ESI-TOF MS. The identified 37 compounds comprised flavanol monomers and oligomers, as well as varieties of ellagitannin components. Treatment of lymphocytes with RSEs induced a significant decrease in the frequency of micronuclei by 80%. These results demonstrate that the constituents of RSEs may be important in the prevention of oxidative lymphocyte damage by reactive oxygen species and may also reduce the level of DNA damage. These findings support the potential benefits of polyphenolic compounds from raspberry seeds as efficient antioxidants. 相似文献
80.
Pleines I Elvers M Strehl A Pozgajova M Varga-Szabo D May F Chrostek-Grashoff A Brakebusch C Nieswandt B 《Pflügers Archiv : European journal of physiology》2009,457(5):1173-1185
Platelet activation at sites of vascular injury is triggered through different signaling pathways leading to activation of
phospholipase (PL) Cβ or PLCγ2. Active PLCs trigger Ca2+ mobilization and entry, which is a prerequisite for adhesion, secretion, and thrombus formation. PLCβ isoenzymes are activated
downstream of G protein-coupled receptors (GPCRs), whereas PLCγ2 is activated downstream of immunoreceptor tyrosine-based activation motif (ITAM)-coupled receptors, such as the major platelet collagen receptor glycoprotein (GP) VI or CLEC-2. The mechanisms underlying
PLC regulation are not fully understood. An involvement of small GTPases of the Rho family (Rho, Rac, Cdc42) in PLC activation
has been proposed but this has not been investigated in platelets. We here show that murine platelets lacking Rac1 display
severely impaired GPVI- or CLEC-2-dependent activation and aggregation. This defect was associated with impaired production
of inositol 1,4,5-trisphosphate (IP3) and intracellular calcium mobilization suggesting inappropriate activation of PLCγ2 despite normal tyrosine phosphorylation
of the enzyme. Rac1
−/−
platelets displayed defective thrombus formation on collagen under flow conditions which could be fully restored by co-infusion
of ADP and the TxA2 analog U46619, indicating that impaired GPVI-, but not G-protein signaling, was responsible for the observed defect. In line
with this, Rac1
−/−
mice were protected in two collagen-dependent arterial thrombosis models. Together, these results demonstrate that Rac1 is
essential for ITAM-dependent PLCγ2 activation in platelets and that this is critical for thrombus formation in vivo.
Irina Pleines, Margitta Elvers, Amrei Strehl: these authors contributed equally to this work. 相似文献