Validation of a Chinese version of disease specific quality of life scale (HFS-36) for hemifacial spasm in Taiwan

Background and object  

There was no Chinese questionnaire to evaluate the health-related quality of life (HRQoL) in patients with hemifacial spasm (HFS). In this study, we aimed to validate a new disease-specific HRQoL scale for HFS (HFS-36) in Chinese version, and compared it to SF-36, a generic HRQoL scale.     

长扫描深度谱域OCT测量圆锥角膜患者全角膜厚度的重复性和再现性

目的探讨长扫描深度谱域OCT（UL-OCT）N4量圆锥角膜患者的全角膜三维厚度分布的重复性与再现性。方法前瞻性研究。选取圆锥角膜患者10例（10眼）,使用自行搭建的UL-OCT获得角膜的横断面图像．采用以角膜顶点为中心进行均等放射状B型扫描获取i维图像。通过全自动分割算法获取10mm直径范围角膜厚度分布图,并将测量结果与Pentacam三维眼前节分析诊断系统进行对比。2个仪器的重复性与再现性通过重复性系数与再现性系数（CoR）、组内相关系数（ICC）和变异系数（CV）进行比较。2个仪器的一致性通过配对t检验和Bland-Ahman方法进行分析。结果对于圆锥角膜患者,UL-OCT测量值的重复性系数为3．43～4．31μm,再现性系数为3．58～8．10μm。重复性和再现性的组内相关系数分别为0．998和0．995以上。Pentacam测量值的重复性系数为8．63～14．30μm,再现性系数为14．59～19．17μm。重复性和再现性的组内相关系数分别为0．997和0．994以上。UL-OCT系统测量的角膜厚度在中央区、旁中央区、过渡区、周围区分别为（468．9±38．6）μm、（482．5±48．6）μm、（515．5±57．0）μm、（588．0±52．5）μm。Pentacam系统测量的各区角膜厚度分别为（500．9＋50．0）μm、（533．9＋49．7）μm、（587．9±60．6）μm、（635．3±62．0）μm。Bland-Altman分析角膜厚度测量结果显示,2种仪器测量结果差值的95％一致性界限在中央区、旁中央区、过渡区分别为-44．49～108．59μm、-7．47～110．23μm、-13．21～131．68μm。结论在测量圆锥角膜患者全角膜厚度时,与Pentacam系统相比,UL-OCT系统具有更好的重复性和再现性。UL-OCT系统与Pentacam系统得到的角膜厚度测量结果存在差异,因此2种仪器在临床应用时不可互换。     

An observational study of clinical recurrence in patients with interstitial lung disease related to the antisynthetase syndrome

Clinical Rheumatology - To describe the clinical characteristics and risk factors of clinical recurrence in interstitial lung disease related to antisynthetase syndrome (ARS-ILD). Patients...     

The anti-metastatic efficacy of β-ionone and the possible mechanisms of action in human hepatocarcinoma SK-Hep-1 cells

β-Ionone (BI), a precursor for carotenoids, is widely distributed in fruit and vegetables. Recent in vitro studies have demonstrated the potential anti-metastatic effects of BI, but the mechanisms underlying such actions are not clear. Because liver cancer is the most endemic cancer in Taiwan and in a large region of the world, we hereby investigate the anti-metastatic effects of BI and its mechanisms of actions in a highly metastatic human hepatocarcinoma SK-Hep-1 cells. We show that incubation of cells with BI (1-50?μm) for 24 and 48?h significantly inhibited cell invasion, migration and adhesion. Mechanistically, incubation of cells with BI (1-50?μm) for 24?h resulted in the following: (1) significant inhibition of matrix metalloproteinase (MMP)-2, MMP-9 and urokinase-type plasminogen activator activities, (2) up-regulation of protein expression of the tissue inhibitor of matrix metalloproteinase (TIMP)-1, TIMP-2 and plasminogen activator inhibitor-1, (3) down-regulation of the expression of migration-related proteins, including focal adhesion kinase (FAK), phosphorylated form of FAK, Rho, Rac1 and Cdc42 and (4) up-regulation of the expression of nm23-H1 protein (P?相似文献   

缺血性心脏病微血管再生临床研究进展

缺血性心脏病的治疗,首先要解决心肌组织的缺氧和营养物质供应不足。促进缺血心肌区域血管的新生、改善微循环是当前治疗缺血性心脏病的研究方向之一。临床促血管再生的治疗方式包括基因治疗、细胞治疗和药物治疗,目前取得了显著的进展,显示了其广泛的应用前景。     

WDR5 promotes the tumorigenesis of oral squamous cell carcinoma via CARM1/β-catenin axis

Oral squamous cell carcinoma (OSCC) is a malignancy all over the world. WD repeat domain 5 (WDR5) is involved in cancer progression. In addition, it was reported that WDR5 is upregulated in head and neck cancer, while its role in OSCC is unknown. First, the expression of WDR5 in oral cancer tissues and cells was examined by qRT-PCR, IHF and western blot. CCK-8 assay was performed to test the cell viability. Cell migration was assessed by transwell assay. Knocking down WDR5 or CARM1 in oral cancer cells to detect its function on cancer growth, WDR5 and CARM1 were significantly upregulated in OSCC. Silencing WDR5 suppressed OSCC cell viability and migration. CARM1 level in OSCC cells was significantly inhibited by WDR5 downregulation, and CARM1 elevation could rescue the effect of WDR5 knockdown on tumorigenesis of OSCC. Moreover, silencing of WDR5 notably inactivated β-catenin signaling pathway, while this phenomenon was restored by CARM1 overexpression. Silencing of WDR5 attenuated the tumorigenesis of OSCC via CARM1/β-catenin axis. Thus, WDR5 might be a target for OSCC treatment.

     

耳科影像学的关键技术创新和应用——2020年度国家科学技术进步奖二等奖

耳科疾病病变具有解剖位置深、隐匿微小的特征,传统耳科影像学在临床应用时存在看不清、看不准、看不懂的问题,导致病变影像诊断效能低下.王振常教授团队从创建耳科影像的规范检查方案和系统评价模式、提升耳科疾病的病理生理结构显示能力入手,阐明了搏动性耳鸣全链条发生机制,构建耳科影像检查及诊断新体系,并研制十微米级临床耳科电子计算...     

秋水仙碱缓释微丸对痛风性关节炎急性发作的预防作用研究

目的:探讨自制秋水仙碱缓释微丸对痛风性关节炎急性发作模型大鼠的预防作用.方法:采用流化床包衣技术制备秋水仙碱缓释微丸.将24只SD大鼠随机分为正常组、模型组、片剂组和缓释微丸组,每组6只.片剂组将秋水仙碱片研磨成粉,水溶后灌胃给药,缓释微丸组通过特制的灌胃器给予秋水仙碱缓释微丸,正常组和模型组给予等量生理盐水.给药14...     

高效液相色谱法同时测定加味藿香正气软胶囊中橙皮苷、厚朴酚、和厚朴酚和甘草苷的含量

目的：建立高效液相色谱法同时测定加味藿香正气软胶囊中橙皮苷、厚朴酚、和厚朴酚和甘草苷的方法。方法：采用Kromasil C18色谱柱（250 mm×4.6 mm,5 μm）,以水-甲醇为流动相梯度洗脱,流速1.0 mL·min-1,检测波长287 nm。结果：橙皮苷、厚朴酚、和厚朴酚、甘草苷分别在4.47-178.70 μg·mL-1、3.42-136.96 μg·mL-1、3.49-139.48 μg·mL-1、3.92-157.20 μg·mL-1 范围内与峰面积呈良好线性关系（r=0.999 9）,平均回收率分别为99.48%、99.05%、99.57%、99.79%。结论：该方法准确、灵敏、专属性强,为加味藿香正气软胶囊质量标准提高奠定了基础。