Molecular and Serological Evidence for Anaplasma platys and Babesia sp. Infection in a Dog,Imported in Belgium,from Southern Spain

This case report describes a dog suffering from a co‐infection with Babesia and Anaplasma parasites. Anaplasma platys was found to be responsible for the anaplasmosis by molecular biology techniques, while microscopical and serological evidence was found for a coexistent babesiosis, although this could not be confirmed by polymerase chain reaction. Moreover, the possible risk of import of exotic pathogens is highlighted.     

丹参改善骨骼肌缺血再灌注损伤的超微结构观察

目的:通过透射电镜观察骨骼肌缺血再灌注损伤过程的病理生理变化探讨丹参的干预作用及机制。方法:采用90只清洁级雄性SD大鼠,6只作为健康对照组,84只行左侧提睾肌主滋养动脉夹闭造模,在缺血2.5 h时,丹参组腹腔注射丹参(42只),对照组注射等剂量的生理盐水(42只),两组分别设缺血再灌注10、20、30、40、50、60、90 min观测点(每个观测点6只)。观测结束后,迅速切取提睾肌,放入电镜固定液。电镜下进行组织形态学观察。结果:健康对照组正常情况下,组成肌原纤维的粗、细2种肌丝沿其长轴排列,每条肌原纤维上均可见明暗相间的带,肌浆内见有较多糖原颗粒及少量内质网、线粒体等细胞器,肌浆网纵向分布于肌原纤维周围,线粒体膜及嵴清晰可见,基质着色深,无肿胀、空泡形成等改变,在肌丝之间分布有较多糖原颗粒;骨骼肌缺血再灌注后10、20、30、40、50、60、90 min的2组各观测点,其病理变化较相似,肌丝模糊、凌乱,部分溶解、消失,甚至完全消失,肌浆网不同程度扩张,线粒体变大变圆,基质变淡,嵴变短变少甚至消失,部分线粒体极度肿胀,呈空泡状。丹参组,其病理变化大体相似,肌纤维均有不同程度恢复。结论:骨骼肌缺血再灌注损伤导致线粒体极度肿胀,肌纤维紊乱。丹参能有效地消除线粒体的水肿,恢复肌纤维,减轻骨骼肌缺血再灌注损伤。     

颈外静脉穿刺置管在大量输液中的应用

目的探讨静脉药物配置室内不同操作距离对空气微粒污染的影响程度。方法在配置室内模拟配液加药，分别在距离操作者不同位置，用空气粒子计数器对空气微粒数进行检测，以了解空气微粒的污染情况。结果操作距离不同位置5个点的空气微粒污染程度比较，差异有显著性意义（均P〈0.01）。配置室内操作时空气微粒污染程度与操作距离有关，近操作者较远离操作者污染明显；此外，空气微粒污染程度还与洁净室的空间位置有关，回风口微粒污染明显，而送风口污染最轻。结论在万级洁净室内进行配液操作时，操作者周围空气中微粒污染仍严重，必须使用层流台或生物安全柜，以保证配液的质量和增强职业防护。     

Detection of C. pneumoniae by polymerase chain reaction-enzyme immunoassay in abdominal aortic aneurysm walls and its association with rupture.

OBJECTIVE: Serological studies have suggested that one of the risk factors for aneurysm development is C. pneumoniae infection. The purpose of this study was to evaluate whether there is an association between the presence of C. pneumoniae DNA in aneurysms and ruptured abdominal aortic aneurysms. METHODS: Aortic walls were collected consecutively from 30 patients with intact AAA, 16 patients with ruptured AAA and 19 healthy organ donors (control). Purified DNAs from all aortas were analyzed for the presence of C. pneumoniae DNA in parallel by polymerase chain reaction-enzyme immunoassay (PCR-EIA) and agarose gel electrophoresis. PCR-EIA has a high sensitivity in detecting low DNA copy number in clinical atherosclerotic samples. RESULTS: C. pneumoniae DNA was detected more frequently in patients with aneurysms, particular with ruptured aneurysms. The incidence of positive C. pneumoniae DNA was 73.3% in intact AAA and 10.5% in control aortas, with the highest frequency in ruptured AAA (100%) (p < 0.05). CONCLUSION: Giving the high specificity and sensitivity of PCR-EIA, these findings support the association of C. pneumoniae in the pathogenesis of aneurysm development, growth and rupture.     

CO2激光治疗宫颈糜烂2412例疗效观察

目的 探讨CO2激光治疗宫颈糜烂的疗效。方法 采用CO2激光输出功率20-40w，波长10．6μm，针对轻、中、重不同糜烂程度采用不同光束强度，光斑直径约0．3-0．5的连续波治疗宫颈糜烂2412例。结果 术后三个月复查，总有效率100％。结论 CO2激光治疗术简单、安全、疗效满意，适用于治疗I-Ⅲ0宫颈糜烂。     

医源性桡神经损伤46例分析

目的阐述医源性桡神经损伤的伤因、治疗和预防措施。方法分析1991年～2003年间收治的46例医源性桡神经损伤的病例。结果伤因分为上肢手术误伤，肱骨干和桡骨上端闭合性骨折复位手法不当，头静脉穿刺致桡神经浅支损伤。本组21例保守治疗，22例手术治疗，3例行肌腱转位功能重建。结论手术误伤是医源性桡神经损伤的首要原因；临床医生丰富的解剖知识和细致、轻柔的操作是预防医源性桡神经损伤的关键。     

多层螺旋CT和MRI测量肩胛盂斜倾角的互补性初探

目的 探讨多层螺旋CT(MSCT)多平面重组(MPR)及MRI测量肩胛盂斜倾角的互补性.方法 收集成人肩胛骨干标本22侧及肩关节防腐湿标本24侧,用16层螺旋CT扫描仪扫描,多平面重组重建肩胛骨并测量肩胛盂斜倾角,得数据A组.收集MRI检查正常的肩关节病例50侧,取其斜冠状位T1WI显示肩锁关节的层面测量肩胛盂斜倾角,得数据B组.进行统计学处理.结果 肩胛盂斜倾角最小值91.10°,最大值118.50°,A、B 2组肩胛盂斜倾角数据分别为101.14°±4.66°和104.05°±7.72°.2种测量方法肩胛盂斜倾角值统计学上有显著性差异(P＜0.05).结论 MSCT的MPR法测量肩胛盂斜倾角与MRI测量值不同,前者略小于后者;MSCT和MRI 2种测量肩胛盂斜倾角方法结合具有重要的应用价值.     

针灸加推拿刮法治疗胫骨结节骨骺炎16例

胫骨结节骨骺炎是由于股四头肌肌腱对胫骨结节的过度牵拉造成的骨骺疾病，在刚从事运动训练的青少年及刚人体育院校学习的新生中发病率较高，尤其多见于田径、篮球、排球等项目专选的学生。笔者自2000年～2003年中，共收治了16例该病患者，均获满意疗效，现介绍如下。     

Sustained delivery and expression of plasmid DNA based on biodegradable polyester, poly(D,L-lactide-co-4-hydroxy-L-proline).

Gene expression mediated by a non-viral vector usually lasts only a few days. The objective of this study was to synthesize and characterize a non-toxic, polymeric gene carrier, poly(D,L-lactide-co-4-hydroxy-L-proline) (PLHP) for sustained gene delivery. The copolymer was synthesized by ring-opening polymerization of D,L-lactide (DLLA) with N-cbz-4-hydroxy-L-proline (HP) in the presence of stannous octoate (Sn(Oct)(2)). The resulting copolymer was characterized by (1)H nuclear magnetic resonance (NMR) and gel permeation chromatography (GPC). Degradation of PLHP was examined by monitoring the medium pH change and molecular weight (MW) of the remaining polymer. It showed a rapid initial degradation and followed by a slower degradation for about 30 days at 37 degrees C. The cytotoxicity of copolymer was significantly lower than polyethylenimine (PEI) and poly-L-lysine hydrochloride (PLL) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The plasmid DNA (pDNA)-loaded microspheres based on the copolymer were prepared by a water-oil-water (w/o/w) solvent evaporation emulsion method. The release profile of pDNA from PLHP microspheres showed an initial burst release, and then a slower and continuous release for about 18 days at 37 degrees C. Gene transfer efficiency of PLHP/pDNA delivery system showed a sustained activity (over a week) when compared with PEI and PLL, and can be further improved by the addition of cationic liposomes. The results suggest that PLHP is a promising candidate for long-term gene delivery with good biocompatibility and biodegradability.     

新基因Tctex5在雄鼠生殖细胞中的表达

目的探讨雄鼠生殖细胞中是否有Tctex5基因的表达。方法从三株体外培养的雄鼠生殖细胞CRL-1715、CRL-2053和CRL-2196细胞株中提取总RNA和蛋白，采用RT-PCR和Western blot分别从mRNA和蛋白质水平分析基因产物的表达。结果Tctex5基因在CRL-1715、CRL-2053、CRL-2196三株细胞中都有高表达。结论Tctex5基因作为一个未知功能的基因，在雄鼠生殖细胞中有丰富的表达，可能参与了精子发生的功能调节，作为男性正常生育的。个新的标志物，它可能在精子发生中扮演了一个重要的角色，该基因在雄性生殖细胞中的发现可能会对男性不育的诊断与治疗提供新的途径。