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排序方式: 共有2610条查询结果,搜索用时 15 毫秒
91.
Yasuda T Hayakawa H Nihira T Ren YR Nakata Y Nagai M Hattori N Miyake K Takada M Shimada T Mizuno Y Mochizuki H 《Journal of neuropathology and experimental neurology》2011,70(8):686-697
Loss-of-function mutations in the ubiquitin ligase parkin are the major cause of recessively inherited early-onset Parkinson disease (PD). Impairment of parkin activity caused by nitrosative or dopamine-related modifications may also be responsible for the loss of dopaminergic (DA) neurons in sporadic PD. Previous studies have shown that viral vector-mediated delivery of parkin prevented DA neurodegeneration in several animal models, but little is known about the neuroprotective actions of parkin in vivo. Here, we investigated mechanisms of neuroprotection of overexpressed parkin in a modified long-term mouse model of PD using osmotic minipump administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Recombinant adeno-associated viral vector-mediated intranigral delivery of parkin prevented motor deficits and DA cell loss in the mice. Ser129-phosphorylated α-synuclein-immunoreactive cells were increased in the substantia nigra of parkin-treated mice. Moreover, delivery of parkin alleviated the MPTP-induced decrease of the active phosphorylated form of Akt. On the other hand, upregulation of p53 and mitochondrial alterations induced by chronic MPTP administration were barely suppressed by parkin. These results suggest that the neuroprotective actions of parkin may be impaired in severe PD. 相似文献
92.
Toshiyuki Ko Yuko Utanohara Yasuhiro Suzuki Makiko Kurihara Nobuo Iguchi Jun Umemura Tetsuya Sumiyoshi Hitonobu Tomoike 《Heart and vessels》2016,31(1):38-45
Simultaneous dual-isotope SPECT imaging with 201Tl and 123I-β-methyl-p-iodophenylpentadecanoic acid (BMIPP) is used to study the perfusion–metabolism mismatch. It predicts post-ischemic functional recovery by detecting stunned myocardium. On the other hand, 99mTc-MIBI is another radioisotope widely used in myocardial perfusion imaging because of its better image quality and lower radiation exposure than 201Tl. However, since the photopeak energies of 99mTc and 123I are very similar, crosstalk hampers the simultaneous use of these two radioisotopes. To overcome this problem, we conducted simultaneous dual-isotope imaging study using the D-SPECT scanner (Spectrum-Dynamics, Israel) which has a novel detector design and excellent energy resolution. We first conducted a basic experiment using cardiac phantom to simulate the condition of normal perfusion and impaired fatty acid metabolism. Subsequently, we prospectively recruited 30 consecutive patients who underwent successful percutaneous coronary intervention for acute myocardial infarction, and performed 99mTc-MIBI/123I-BMIPP dual-isotope imaging within 5 days after reperfusion. Images were interpreted by two experienced cardiovascular radiologists to identify the infarcted and stunned areas based on the coronary artery territories. As a result, cardiac phantom experiment revealed no significant crosstalk between 99mTc and 123I. In the subsequent clinical study, 99mTc-MIBI/123I-BMIPP dual-isotope imaging in all participant yielded excellent image quality and detected infarcted and stunned areas correctly when compared with coronary angiographic findings. Furthermore, we were able to reduce radiation exposure to significantly approximately one-eighth. In conclusion, we successfully demonstrated the practical application of simultaneous assessment of myocardial perfusion and fatty acid metabolism by 99mTc-MIBI and 123I-BMIPP using a D-SPECT cardiac scanner. Compared with conventional 201TlCl/123I-BMIPP dual-isotope imaging, the use of 99mTc-MIBI instead of 201TlCl improves image quality as well as lowers radiation exposure. 相似文献
93.
Takuma Kohsaka Takaaki Hiragun Kaori Ishii Makiko Hiragun Akiko Kamegashira Michihiro Hide 《Allergology international》2018,67(1):103-108
Background
Atopic dermatitis (AD) is exacerbated by sweating, and the skin of most patients with AD are resided by Malassezia (M.) fungi. Recently, MGL_1304 produced by Malassezia globosa was identified as the major histamine releasing antigen in human sweat.Methods
The full length cDNA of the counterpart of MGL_1304 in Malassezia restricta (Mala r 8), was cloned by degenerate PCR and rapid identification of cDNA ends (RACE). Recombinant MGL_1304, and its counterparts, Mala s 8 (produced by Malassezia sympodialis) and Mala r 8 were prepared, and compared in their allergenicities by dot blot analysis and histamine release tests with sera and basophils of patients with AD.Results
The identities between MGL_1304 and Mala s 8, MGL_1304 and Mala r 8, and Mala s 8 and Mala r 8 were 68%, 78%, and 76%, respectively, in protein sequences. Dot blot analysis revealed that the level of IgE binding to Mala s 8 was higher than that of MGL_1304. However, histamine release tests revealed that MGL_1304 and Mala r 8 possessed higher activity than Mala s 8. In addition, the crude lysate of M. globosa showed higher histamine release ability than that of M. sympodialis.Conclusions
Patients with AD showed hypersensitivities against MGL_1304 and its homologs. However, the allergenicities of the homologs are variable and the histamine release activities may be different from the solid-phase binding activities for IgE. Sweat allergy should be carefully evaluated with biological activities of MGL_1304 and its homologs of other Malassezia fungi residing on the skin. 相似文献94.
Radical polymerization of 12‐[(N‐methacryloyl)carbamoyloxy] octadecanoic acid ( 1 ) was kinetically and ESR spectroscopically investigated in acetone, using dimethyl 2,2′‐azobisisobutyrate ( 2 ) as initiator. The polymerization rate (Rp) is given by Rp = k [2]0.7[1]1.4 at 50°C. Propagating poly( 1 ) radical was observed as a 13‐lines spectrum by ESR under the actual polymerization conditions. The ESR‐determined kp values (1.8–7.9 L/mol·s) are much lower than those of usual methacrylate monomers. The rate constant (kt) of termination was determined to be kt = 1.0–2.7·104 L/mol·s from decay curve of the propagating radical. The Arrhenius plots of kp and kt gave the activation energies of propagation (63 kJ/mol) and termination (24 kJ/mol). A significant solvent effect was observed on the radical polymerization of 1 . The copolymerizations of 1 with styrene(St) and acrylonitrile were examined at 50°C. Copolymerization parameters obtained for the 1 (M1)/St (M2) system are as follows; r1 = 0.73, r2 = 0.57, Q1 = 0.83, and e1 = 0.13. 相似文献
95.
Yoshiro Horai Oka Satoru Samuel Lapalme-Remis Remi Sumiyoshi Yoshikazu Nakashima Takahisa Suzuki Akitomo Okada Shin-ya Kawashiri Kunihiro Ichinose Mami Tamai Satoshi Yamasaki Hideki Nakamura Fuminao Takeshima Tomoki Origuchi Atsushi Kawakami 《Modern rheumatology / the Japan Rheumatism Association》2013,23(3):572-576
A 22-year-old female with ulcerative colitis that was successfully treated with infliximab (IFX), and remained stable following tapered discontinuation of prednisolone, developed anterior neck pain and elevation of C-reactive protein following her fourth administration of IFX. She was diagnosed with Takayasu arteritis (TA) based on neck ultrasound and computed tomography angiography. This is the first report describing the development of TA during treatment of UC with IFX. 相似文献
96.
We prospectively studied the dose‐dependent effect of transfused stored red blood cells (RBCs) on recipient RBC indices, deformability and cell density in 10 patients administered stored RBCs for blood transfusion during general surgery. There were dose‐dependent decreases in mean corpuscular volume and increases in mean corpuscular haemoglobin concentration after completion of 4‐ and 6‐unit stored RBC transfusions. The amount of dense populations increased proportionately with the amount of stored RBCs transfused. The maximal deformability index value was significantly and dose‐dependently decreased, suggesting that hemodynamic blood flow, especially the microcirculation may be impaired in patients who receive large amounts of stored RBCs. 相似文献
97.
Makiko Yoshida Yuji Takahashi Asako Koike Yoko Fukuda Jun Goto Shoji Tsuji 《Human mutation》2010,31(9):1003-1010
An amyotrophic lateral sclerosis (ALS) mutation database has been constructed as a publicly accessible online resource for recording the nucleotide and amino acid variants identified in genes associated with ALS, along with corresponding clinical conditions. The database currently consists of more than 600 entries, including about 180 unique variants found in 25 disease‐causative or disease‐related genes. In addition to published data collected from literature, novel variants identified by microarray resequencing in our laboratory are incorporated into the database. Every reported gene has a respective page that provides information on its variation positions with various statistics, clinical characteristics, and primary references, as well as gene‐sequence and protein‐structure information that will assist in assessing variation significance. Users can access a homology search function to find variations in arbitrary sequences of interest and to check if they have already been described in the database. This database is expected to fulfill an essential need in terms of integrating comprehensive information on genetic and clinical data related to ALS, which will subsequently deepen our understanding of the possible mechanisms of the disease, as well as help with the clinical practice and treatment of ALS. The database is accessible at: https://reseq.lifesciencedb.jp/resequence/SearchDisease.do?targetId=1 . Data submission is open to all researchers and is highly encouraged. Hum Mutat 31:1003–1010, 2010. © 2010 Wiley‐Liss, Inc. 相似文献
98.
Expression of interferon regulatory factor 7 correlates with the expression of Epstein–Barr Virus latent membrane protein 1 and cervical lymph node metastasis in nasopharyngeal cancer 下载免费PDF全文
99.
Hua Tang Kimi Ariki Makiko Ohkido Yasuko Murakami Senya Matsufuji Zhenghua Li Ken-ichi Yamamura 《Genes to cells : devoted to molecular & cellular mechanisms》2009,14(1):79-87
Ornithine decarboxylase (ODC) antizyme inhibitor (AZI) has been shown to regulate ODC activity in cell cultures. However, its biological functions in an organism remain unknown. An embryonic stem (ES) cell clone was established, in which the Azin1 gene was disrupted by the gene trap technique. To identify the function of Azin1 gene in vivo , a mutant mouse line was generated using these trapped ES cells. Homozygous mutant mice died at P0 with abnormal liver morphology. Further analysis indicated that the deletion of Azin1 in homozygous mice resulted in the degradation of ODC, and reduced the biosynthesis of putrescine and spermidine. Our results thus show that AZI plays an important role in regulating the levels of ODC, putrescine and spermidine in mice, and is essential for the survival of mice. 相似文献
100.
Junji Takaya Yuri Fujii Hirohiko Higashino Shoichiro Taniuchi Makiko Nakamura and Kazunari Kaneko 《Pediatric diabetes》2009,10(7):484-486
Abstract: The WHIM syndrome is a rare immunological disorder characterized by warts, hypogammaglobulinemia, infections, and myelokathexis. We hypothesized that immunological or genetic mechanisms may link WHIM syndrome and type 1 diabetes. We report that the young girl with WHIM syndrome developed diabetes and transient hypothyroidism. A nonsense mutation (C→T) truncating the CXC chemokine receptor 4 (CXCR4) C-terminal cytoplasmic tail domain occurred at nucleotide position 1000(R334X) of the CXCR4 gene in one allele of the patient was identified, and the person was diagnosed as having WHIM syndrome. Recent observation suggested that the CXCR4, a G-protein-coupled receptor with a unique ligand, CXCL12, might be involved in the pathogenesis for type 1 diabetes. Taken into consideration the concurrent prevalence of the two disorders and the speculated common pathogenesis associated with the CXCR4, our patient may enable us to understand the genetic damage related to accelerated apoptosis. 相似文献