慢性非萎缩性胃炎与幽门螺杆菌感染的相关性分析

目的探讨慢性非萎缩性胃炎的胃黏膜炎症的程度、部位、活动性与幽门螺杆菌(Helicobacter pylori,Hp)感染的关系,为临床胃炎抗Hp治疗提供理论依据。方法对胃镜诊断为慢性非萎缩性胃炎的165例患者行活检病理诊断及Hp检测,然后对资料进行分析整理。结果 165例慢性非萎缩性胃患者Hp感染率为41.82%,与性别、胃黏膜炎症的程度、活动性均呈显著正相关(P<0.01),但与年龄、炎症部位差异均无统计学意义(P>0.05)。结论 Hp感染与慢性非萎缩性胃炎的程度、活动性有关,慢性非萎缩性胃炎有必要作抗Hp根除治疗。     

Mononuclear-cell subsets in human idiopathic crescentic glomerulonephritis (ICGN): Analysis in tissue sections with monoclonal antibodies

Mononuclear inflammatory cells (MIC) were analyzed in renal biopsies from 16 patients with ICGN (7 with glomerular immune complex deposits, 3 with anti-GBM disease, and 6 without immune deposits) by the avidin-biotin-immunoperoxidase technique utilizing monoclonal antibodies to cell surface antigens: T11 (total T), T4 (inducer/helper T), T8 (suppressor/cytotoxic T), B1 (B cells), M1 (monocytes/granulocytes), and Leu 7 [natural killer (NK) cells]. Total MIC were significantly increased in both glomeruli and interstitial tissues of the patients. Interstitial MIC consisted mainly of lymphocytes (80%) and monocytes (19%), with small numbers of B and NK cells present. In contrast, MIC in renal glomeruli of patients with ICGN were composed of monocytes (65%) rather than T lymphocytes (34%). A majority of T lymphocytes found in renal tissues of patients and controls had the helper/inducer phenotype. Tissue T4/T8 ratios were not significantly different in the glomeruli and interstitium. Monocytes and T lymphocytes accumulating in renal tissues of patients with ICGN may mediate glomerular injury in all forms of human ICGN.Presented in part at the 15th Annual Meeting of the American Society of Nephrology, Chicago, Illinois, 1982.     

钛膜支架植入联合鼻腔底黏骨膜瓣修补鼻中隔大穿孔(附11例报告)

目的：探讨应用医用超薄钛膜作支架植入联合鼻腔底带蒂黏膜瓣修补中等以上鼻中隔穿孔的效果。方法：自一侧鼻腔底部作一黏骨膜瓣以鼻中隔穿孔下缘为蒂经穿孔处翻向对侧,于黏骨膜下嵌入式植入一块约大于穿孔的超薄钛膜支架封闭穿孔,并缝合固定。结果：11例患者鼻中隔穿孔均1次修补成功,鼻中隔基本平直,鼻腔通气良好,随访6～12个月,无排异反应及鼻中隔穿孔。结论：钛膜极薄,具有良好的组织相容性,可作为支架植入修补较大的鼻中隔穿孔。     

New Residue Analysis Method for Four Task-Specific Ionic Liquids in Water,Soil and Plants

Bulletin of Environmental Contamination and Toxicology - With the extensive application of task-specific ionic liquids (TSILs), their environmental impact has attracted increasing attention....     

合作医疗对卫生服务利用的比较研究——新疆农村贫困地区合作医疗调研报告

《中共中央，国务院关于卫生改革与发展的决定》中明确提出，积极稳妥地发展和完善合作医疗制度是加强我国农村卫生工作的重点之一，然而，时至今日，还有部分地区的领导对合作医疗制度在保证农民获得基本医疗服务，落实预防保健任务，缓解因病致贫等方面的积极作用持怀疑态度，在整个农村经济中的地位和作用如何？是减轻了农民负担，还是加重了农民负担？是提高了贫困农民对基本医疗服务的可及性，还是相反？我们带着这些问题，在联合国儿童基金会的资助下，于2000年10月31日至11月6日对我国经济较为落后的新疆喀什地区岳普湖，麦盖提和莎车3个县进行了调研。     

中药现代化进展及存在问题

本文从中药农业、中药工业、中药商业和中药知识产权等4个方面概述了中药产业的现状。作者认为：中药材生产在建立GAP基地,保证药材的质量的同时,应采用先进的生物工程技术开辟中药材生产的新模式;中药工业应引入先进制剂技术中药,以生产出“三效”、“三小”、“五方便”的现代中药;药材专业市场的规范管理应发挥更大的作用,中药商业应实行代理制模式积极开拓国际市场;中药企业的知识产权保护,应从专利、商标、著作权等多方位着于,构建中药知识产权的立体保护体系。     

Inflammatory cytokine–regulated tRNA-derived fragment tRF-21 suppresses pancreatic ductal adenocarcinoma progression

The tumorigenic mechanism for pancreatic ductal adenocarcinoma (PDAC) is not clear, although chronic inflammation is implicated. Here, we identified an inflammatory cytokine–regulated transfer RNA–derived (tRNA-derived) fragment, tRF-21-VBY9PYKHD (tRF-21), as a tumor suppressor in PDAC progression. We found that the biogenesis of tRF-21 could be inhibited by leukemia inhibitory factor and IL-6 via the splicing factor SRSF5. Reduced tRF-21 promoted AKT2/1-mediated heterogeneous nuclear ribonucleoprotein L (hnRNP L) phosphorylation, enhancing hnRNP L to interact with dead-box helicase 17 (DDX17) to form an alternative splicing complex. The provoked hnRNP L-DDX17 activity preferentially spliced Caspase 9 and mH2A1 pre-mRNAs to form Caspase 9b and mH2A1.2, promoting PDAC cell malignant phenotypes. The tRF-21 levels were significantly lower in PDACs than in normal tissues, and patients with low tRF-21 levels had a poor prognosis. Treatment of mouse PDAC xenografts or patient-derived xenografts (PDXs) with tRF-21 mimics repressed tumor growth and metastasis. These results demonstrate that tRF-21 has a tumor-suppressive effect and is a potential therapeutic agent for PDAC.     

中国10省(市)2009－2014年15岁以下儿童流感确诊住院病例严重急性呼吸道感染临床特征及其危险因素分析

目的 探讨我国<15岁儿童流感确诊病例严重急性呼吸道感染(SARI)临床特征及感染危险因素。方法 分析10省市2009年12月至2014年6月SARI哨点监测系统纳入的<15岁儿童流感实验室确诊住院病例。通过调查表收集病例人口学、既往疾病史、临床症状和体征、治疗和结局等信息,描述临床特征及流感感染危险因素。结果 纳入的2 937例儿童SARI住院病例中,190例(6.5%)为流感确诊病例。流感确诊病例中,男性123例(64.7%),<5岁儿童139例(73.2%),年龄M=3.0(IQR:1.0～5.0)岁。20例(10.5%)至少有一种慢性基础性疾病,其中慢性心血管疾病(3.2%)、免疫抑制性疾病(3.2%)和肿瘤(2.6%)最常见。发热(92.6%)和咳嗽(88.8%)是最常见的临床症状,肺部听诊异常(51.1%)和X线表现肺部异常(36.1%)是最常见的临床体征。29例(15.8%)出现并发症,以肺炎(15.3%)为最常见的并发症。16例(8.6%)服用抗病毒药物,4例(2.2%)收入ICU治疗。危险因素分析提示,<6月龄是流感病毒感染的保护因素(OR=0.406,95%CI:0.203～0.815),5～9岁是流感病毒感染的危险因素(OR=2.535,95%CI:1.059～6.066)。结论 流感确诊病例主要集中在<5岁儿童,不同年龄组病例感染流感病毒的风险存在差异。     

新型农村合作医疗综合评价指标体系建立

目的 建立新型农村合作医疗综合评价指标体系.方法 应用层次分析法和改进的德尔菲法构建新型农村合作医疗综合评价指标体系,并确定评价指标权重.结果 专家平均年龄43岁,平均从事专业时间20年.该轮咨询的回收率为95%,权威程度平均值为0.7522,协调系数有统计学意义（P＜0.05）,权重系数依次为结构0.2500、过程0.2500、结果0.5000.结论 专家的代表性较强,积极性高,权威性、协调性较好,综合评价指标体系可用于评价新型农村合作医疗试点县.     

Enhanced immune response to DNA-based HPV16L1 vaccination by costimulatory molecule B7-2

We have investigated whether co-injection of DNA encoding the costimulatory molecule B7-2 augments immune response to the major capsid protein L1 of the high-risk human papillomavirus type 16 (HPV16L1). While immunoglobulin G (IgG) specific to HPV16L1 was detected in sera from mice injected intramuscularly with pcDNA-L1 that encodes HPV16L1, a significantly increased level of IgG was found in sera from mice immunised with pcDNA-L1 in conjunction with pLXHDmB7-2 DNA. Levels of IgG in the anti-sera were correlated with the inhibitory activity of the murine erythrocyte hemagglutination caused by the virus-like particles (VLP) and the binding of VLP to HeLa cells. Moreover, splenic cells isolated from mice co-injected with pLXHDmB7-2 had stronger proliferation and more IFN-gamma producing T cells (CD4(+) and CD8(+)) when stimulated with HPV16 VLP compared with cells from mice that had received pcDNA-L1 alone and mice of the control groups. Furthermore, in footpad swelling test, mice co-immunised with pLXHDmB7-2 had greater skin thickness over those immunised with pcDNA-L1 alone or control mice. We conclude that co-injection of DNA encoding B7-2 can enhance both humoral and cellular immune responses elicited by DNA-based vaccination against HPV16 infection in mice.