EB病毒编码潜伏膜蛋白1通过转录因子c-Jun和Ets1间信息交流调控鼻咽癌中基质金属蛋白酶9的表达

目的　探讨在EBV -LMP1作用下,基质金属蛋白酶9(MMP 9)启动子区相邻的AP 1( 5 33)和Ets( 5 4 0 )结合位点对其转录活化的影响,并确定LMP1可通过c -Jun和Ets1间信息交流(cross -talk)调控鼻咽癌细胞中MMP 9的表达。方法　应用定点突变技术,在野生型MMP- 9 -CAT质粒的基础上,建立MMP- 9启动子区相邻Ets( 5 4 0 )结合位点和AP- 1 (- 5 33)结合位点单独突变体和共同突变体;在四环素调控表达LMP1的鼻咽癌细胞系L7中,比较LMP1对这些突变体报道基因活性的影响。通过针对c- Jun、Ets1的硫代反义寡核苷酸进行阻断,采用GelatinZymography观察c -Jun、Ets1及其cross- talk对LMP1介导的MMP 9表达的影响。结果　与野生型MMP -9 -CAT质粒比较,突变体的报道基因活性均降低(P <0 .0 1 ) ,以MMP- 9 -CATAP -1 ( 5 33) /Ets( 5 4 0 )mt降低最为显著;在c Jun和Ets1反义寡核苷酸单独或共同阻断后,LMP1介导的MMP -9活性均降低,以共同阻断的作用最为明显。结论　EBV -LMP1可以通过转录因子c -Jun和Ets1间cross talk调控鼻咽癌细胞中MMP 9的表达。     

住院精神病患者服药依从性的影响因素

目的分析住院精神病患者服药依从性的影响因素,从而提出干预措施。方法通过与病人、家属及其他病友交流以及对病人进行观察,对89例服药依从性差的住院患者进行分析。结果影响住院精神病患者服药依从性的原因很多,在89例被调查者中,对疾病无正确认识23例(占25.84%),害怕出现药物副反应17例(占19.10%),受幻觉妄想内容的影响15例(占16.85%),认为自己病好了没有必要再服药11例(占12.36%),对医护人员不信任7例(占7.86%),经济因素7例(占7.86%),家属对病人支持不良6例(占6.74%),企图积存药物自杀3例(占3.37%)。结论影响住院精神病患者服药依从性的原因,有病人个人的因素、医护人员的因素和其他因素(经济因素、家属的态度)等。     

The Effect of Group Consultation on University Freshmen Career Planning

目的 探讨团体心理辅导对大学新生职业规划的影响.方法 大学新生被随机分为实验组和对照组各16名,实验组接受为期3次的团体辅导活动.3周前后对两组学生采用生涯决策量表和团体单元回馈表进行调查分析.结果 ①通过t检验,实验组经过团体辅导后在自我信心、生涯目标方面的得分极其显著高于参加团体辅导前(t=4.74,6.216;P＜0.001);在自我了解、生涯决策方面的得分显著高于参加团体辅导前(t=4.133,3.261;P＜0.01);②通过t检验,经团体心理辅导后的实验组在自我了解程度、自我信心、生涯目标方面极其显著高于对照组(t=-4.152,-5.396,-5.014;P＜0.001).结论 团体辅导对大学新生在职业规划的自我了解程度、自信心、生涯目标、生涯决策方面的影响是积极的,并且效果明显;学生主观反馈良好,针对生涯规划的团体辅导受学生欢迎.     

Clinicopathological study of Japanese patients with genetic iron overload syndromes

In addition to hemochromatosis, aceruloplasminemia and ferroportin disease may be complicated by iron-induced multiple organ damage. Therefore, clinicopathological features should be evaluated in a wider range of genetic iron disorders. This study included 16 Japanese patients with genetic iron overload syndromes. The responsible genes were CP in four, HAMP in one, HJV in three, TFR2 in five, and SLC40A1 in three patients. No phenotype dissociation was observed in patients with the CP, TFR2, or HAMP genotypes. Two of the three patients with the HJV genotype displayed classic hemochromatosis instead of the juvenile type. Patients with the SLC40A1 genotype were affected by mild iron overload (ferroportin A) or severe iron overload (ferroportin B). Transferrin saturation was unusually low in aceruloplasminemia patients. All patients, except those with ferroportin disease, displayed low serum hepcidin-25 levels. Liver pathology showed phenotype-specific changes; isolated parenchymal iron loading in aceruloplasminemia, periportal fibrosis associated with heavy iron overload in both parenchymal and Kupffer cells of ferroportin B, and parenchyma-dominant iron-loading cirrhosis in hemochromatosis. In contrast, diabetes occurred in all phenotypes of aceruloplasminemia, hemochromatosis, and ferroportin disease B. In conclusion, clinicopathological features were partially characterized in Japanese patients with genetic iron overload syndromes.     

Association of eHealth Literacy With Colorectal Cancer Knowledge and Screening Practice Among Internet Users in Japan

Background

In rapidly developing Internet-user societies, eHealth literacy has become important in promoting wellness. Although previous studies have observed that poor health literacy is associated with less knowledge and screening practice of colorectal cancer (CRC), little is known about whether eHealth literacy is associated with these variables.

Objective

The present study examined associations between eHealth literacy, knowledge of CRC, and CRC screening practices.

Methods

Data were analyzed for 2970 Japanese adults (men, 49.9%; mean age ± SD, 39.7 ± 10.9 years) who responded to an Internet-based cross-sectional survey. Knowledge of the definition of CRC, its risk factors and screening practice, previous experience of CRC screening, score on the Japanese version of the eHEALS (J-eHEALS), sociodemographic attributes (sex, age, marital status, educational attainment, and household income level), and frequency of Internet usage were obtained. Sociodemographic attributes and frequency of Internet usage were used as control variables in the multiple regression and logistic regression models.

Results

eHealth literacy was positively associated with CRC knowledge (β = .116, < .001), when the covariables of both eHealth literacy and CRC knowledge were used in the multiple regression model. Moreover, after controlling for sociodemographic factors, which were significantly associated with eHealth literacy and CRC screening practice, an increase of 1 point in the eHEALS score signified that participants were 1.03 times (95% CI = 1.01–1.05) more likely to undergo CRC screening.

Conclusions

Internet users with high eHealth literacy are more likely to have knowledge and previous screening practice related to CRC compared to those with low eHealth literacy.     

骨水泥型人工股骨头置换治疗高龄陈旧股骨颈骨折

目的评估骨水泥型人工双极股骨头置换术治疗老年陈旧性股骨颈骨折的临床疗效。方法 2005年1月至2008年1月,应用骨水泥型人工双极股骨头置换术治疗老年陈旧性股骨颈骨折30例。术后对骨质疏松症进行治疗。根据H arris评分、X线片以及测量骨密度对临床效果进行评估。结果 28例患者获得完整随访,2例患者在随访过程中死亡,随访时间2.3~4.7年,平均3.5年。H arris评分由术后的平均71分（68~80分）提高到末次随访的平均82分（平均74~85分）,优良率为78.6%。术后12个月患者股骨假体周围RO I 1区、RO I 2区、RO I 6区和RO I 7区骨密度变化率差异有统计学意义（P〈0.01）。X线检查未见假体移位及周围出现透亮带,无病例需要翻修。结论骨水泥型人工双极股骨头置换术是治疗高龄陈旧性股骨颈骨折的可行方法,但是应做好术前准备及注意术中细节处理,术后需要治疗骨质疏松症。     

基质金属蛋白酶-13在骨性关节炎发病中的活性调控研究

目的 研究一氧化氮(NO)是否通过膜型基质金属蛋白酶-1(MT1-MMP)间接激活基质金属蛋白酶-13酶原(pro-MMP-13).方法 购买并传代人软骨肉瘤细胞(SW1353),用NO供体S-亚硝基-N-乙酰基青霉胺(SNAP),SNAP+NO清除荆氧合血红蛋白(OxyHb)和SNAP+组织金属蛋白酶抑制物-2(TI...