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101.
102.
Although many monoclonal antibodies have been made in human colon cancer, none of them are from the Chinese species. Recently, a colon cancer cell line CC-M2 established from a Chinese patient has been completely characterized and used as immunogen to produce monoclonal antibodies. Monoclonal antibodies were produced by standard hybridoma technique. The fusion rate was 95.8%. An isotype IgG1 of high proliferation named as Sam-2 was used in this study. The titers were measured around 10(4). Further studies on MoAb Sam-2 through indirect immunofluorescent and immunoperoxidase tests revealed its good specificity and sensitivity in colorectal cancer tissue. In CEA study, the result indicated that Sam-2 may react on a non-CEA related antigen. For further clinical application, the antigen was identified as a glycoprotein by chemical resistant test. In preliminary studies using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting techniques, Sam-2 could recognize two closed antigens or a dimer antigen with molecular weight 25.2 and 27 Kd respectively. 相似文献
103.
104.
Serial peritoneal macrophage function studies in new and established continuous ambulatory peritoneal dialysis patients 总被引:1,自引:0,他引:1
Peritoneal macrophages (PM) perform first-line defense activity against peritonitis, the most important complication in continuous ambulatory peritoneal dialysis (CAPD) therapy. Our longitudinal study has compared the PM function in 20 uremic patients during periods free of peritonitis since they started CAPD therapy in January 1987. The results showed that at the initiation of CAPD, there was a higher bactericidal activity, phagocytosis index, H2O2 production and interleukin-1 (IL-1), gamma-interferon (IFN-gamma) and tumor necrosis factor (TNF) production ability and MHC expression. As time went on, these progressively decreased, and by 9 months after CAPD therapy had started they were significantly lower than at the beginning. During the 1.5-year follow-up period, there was a significantly increased peritonitis rate in the period 6 months after the beginning of CAPD than in the period before the 6th month (88.3 vs. 11.7% respectively; p less than 0.001). These results indicate that PM of new CAPD patients have a more active function than those of established patients. The established patients had a greater risk of peritonitis. A comparison of the immunological profiles of PM from patients who had a peritonitis history shows that phagocytosis index, bactericidal activity and IL-1 and TNF production of PM were significantly decreased during the period free of peritonitis. This result suggests that these parameters may serve as an indicator in developing peritonitis. 相似文献
105.
用辣根过氧化物酶(HRP)微电泳技术研究大鼠前脑和脑干向中央上核(CS)的纤维投射。将HRP输入CS后,观察到缰核、脚间核和被盖背核区有较多的标记细胞,斜角带核、下丘脑核和乳头体核有少量标记细胞。在外侧缰核、脚间核和被盖背核区可见丰富的顺行标记终末。结果表明,前脑的缰核和脑干的脚间核和被盖背核区是CS的主要传入来源,它们之间存在往返的纤维联系。 相似文献
106.
N. H. Chow Y. L. Guo J. S. Lin J. H. Su T. S. Tzai H. R. Guo I. J. Su 《British journal of cancer》1997,75(11):1708-1710
A high incidence of bladder cancer has been documented in an area of chronic arsenic (As) exposure. This study investigates the characteristics of As-associated (n = 49) and other (n = 64) bladder cancers. A higher histological grading was observed for the As-exposed tumours (P = 0.04), but no other difference in pathobiological features or prognosis was found between the two groups. 相似文献
107.
单波长荧光分光光度计测定细胞内Ca^2+浓度的方法探索 总被引:2,自引:1,他引:1
在使用单波长荧光分光光度计测定细胞内游离钙浓度时,通过快速(4~6s)手动转换激发波长(EX),分别测定EX340和380nm时的荧光强度变化,并计算出340nm与380nm时的荧光强度比率(R),然后也采用双波长荧光分光光度计测定细胞内Ca2+浓度的计算公式计算细胞内游离钙浓度。结果显示单波长荧光分光光度计按比例法测得的细胞内游离Ca2+浓度与使用双波长荧光分光光度计测得的结果相似。 相似文献
108.
109.
肺癌患者红细胞免疫功能和T淋巴细胞亚群的检测及临床意义 总被引:10,自引:0,他引:10
作者对62例肺癌患者进行红细胞免疫功能及T淋巴细胞亚群测定,并与20例正常人对照。结果显示:肺癌组红细胞膜C3b受体活性(RBC-C3bRR)、CD3 、CD4 、CD4 /CD8 比值均低于正常人(P<0.05~0.01),红细胞膜的吸附免疫复合物(RBC-ICR)、CD8 均高于正常人(P<0.05~0.01),因此认为红细胞免疫及T淋巴细胞亚群测定对肺癌的诊断、治疗及病情预后估计有一定价值。 相似文献
110.
铜绿假单胞菌全细胞脂肪酸气相色谱分析及应用 总被引:5,自引:0,他引:5
本研究用计算机控制,可程序升温的毛细管柱气相色谱(GC)仪分析48株铜绿假单胞菌标准株和临床株、部分常见假单胞菌、肠杆菌的细胞脂肪酸。结果表明:月桂烯酸(C12:1),月桂酸(C12:0)、十三碳烯酸(C13:1)、十三碳酸(C13:0)、肉豆劳动脑酸(C14:1)、十七碳稀酸(C17:1)、十七碳酸(C17:0)、硬脂酸(C18:0)和花生四烯酸(C20:4)是铜绿包菌有鉴别意义的脂肪酸,组成与 相似文献