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81.
间苯三酚与硫酸镁治疗先兆早产的临床效果比较 总被引:1,自引:0,他引:1
目的比较间苯三酚及硫酸镁治疗先兆早产的临床效果。方法对60例诊断为先兆早产的孕妇作为研究组,给予间苯三酚治疗,并选择43例符合相同条件的病例作为对照组,给予硫酸镁治疗。结果应用间苯三酚及硫酸镁治疗治疗能使有效率分别达90.0%和88.4%,延长孕周至37周数达83.3%和83.7%,两者疗效比较无显著性差异(P〉0.05),但间苯三酚副作用较硫酸镁少。结论间苯三酚能有效抑制宫缩,是治疗先兆早产有效而且安全和方便的方法。 相似文献
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83.
目的:探讨甲基转移酶抑制剂——5-氮脱氧胞苷(5-aza-2′-deoxycytidine,5-aza-dC)和组蛋白去乙酰基酶抑制剂——曲古抑菌素A(trichostatin A,TSA)影响供核细胞H19基因表达的时间依赖性和浓度依赖性。方法:用不同浓度5-aza-dC及TSA按不同作用时间处理供核细胞(鼠尾尖成纤维样细胞),利用实时定量PCR法检测处理后H19基因的表达。结果:不同浓度的5-aza-dC处理组之间H19的表达无明显差异,但延长作用时间可使H19表达增强;TSA使H19的表达下降,但无时间依赖性。结论:长时间低浓度的5-aza-dC能增强供核细胞中H19基因的表达,而TSA则使H19的表达减弱,为体细胞克隆中表观遗传修饰抑制剂的选择及后续基因印迹研究提供了依据。 相似文献
84.
BackgroundADP ribosylation factor 6 (ARF6) is a member of the Rat sarcoma virus (RAS) superfamily that is involved in the regulation of vesicular trafficking, membrane lipid remodeling, and signaling pathways. Our earlier work discovered that ARF6, as a downstream effector of the Kirsten rat sarcoma viral oncogene (Kras)/extracellular signal-regulated kinases (ERK) signaling pathway, may increase proliferation and induce the Warburg effect in gastric cancer (GC) cells. Additionally, ARF6 appears to be a potential biomarker for predicting the prognosis of GC. Ferroptosis has recently been described as a type of nonapoptotic iron-dependent cell death that is strongly associated with the Kras mutation. Therefore, it is critical to continue investigating the link between ARF6 and ferroptosis.MethodsWe first created ARF6 silenced cancer cell lines with lentivirus transfection. The knockdown efficiency was confirmed through quantitative polymerase chain reaction (qPCR) and western blotting. Subsequently, we used Cell Counting Kit-8 (CCK-8) and malondialdehyde (MDA) assay for lipid peroxidation measurement. Following this, qPCR and western blotting were conducted to clarify the mechanism involved. Finally, immunohistochemistry was used to stain human GC samples.ResultsOur findings established that, whereas ARF6 did not directly regulate lipid peroxidation, it did render GC cells susceptible to oxidative stress, particularly erastin-induced lipid peroxidation. Additionally, our research demonstrated that ARF6 may control capecitabine resistance via several routes.ConclusionsARF6 may play a critical role in the development of GC. 相似文献
85.
BackgroundThe factors affecting the postoperative survival of patients with primary appendiceal cancer (PAC) have yet to be fully explored. And there are no clear guidelines for adjuvant treatment after appendectomy. Whether chemotherapy can prolong patient survival after appendectomy, is critical in guiding postoperative medications. The majority of studies on appendiceal cancer are single case reports, and they focused on the incidence of appendiceal cancer. The present study aimed to investigate the survival characteristics of patients with primary appendiceal cancer after surgery using the Surveillance, Epidemiology, and End Results (SEER) database.MethodsThe data of 2,891 cases of primary appendiceal cancer between 2004 to 2015 were obtained from the SEER database and subjected to survival analysis using the Kaplan-Meier method and Cox proportional-hazards model. The annual percentage change (APC) was calculated using the weighted least squares method.ResultsThe overall age-adjusted incidence rate per 100,000 population steadily increased from 0.58 in 2004 to 1.63 in 2015. For patients who received chemotherapy, the median overall survival (OS) was 65 months and the 5-year OS rate was 51.9%, and for patients who did not receive chemotherapy or whose chemotherapy status was unknown, the median OS was not reached and the 5-year OS rate was 78.9%. Age [35< age <69: hazard radio (HR) =2.147; 95% confidence interval (CI): 1.442–3.197, P<0.001; age >69: HR =5.259; 95% CI: 3.485–7.937, P<0.001], race (White race: HR =0.728; 95% CI: 0.590–0.899, P=0.003), histologic type (mucinous neoplasm: HR =0.690; 95% CI: 0.580–0.821, P<0.001; malignant carcinoid: HR =0.657; 95% CI: 0.536–0.806, P<0.001), grade (II: HR =1.794; 95% CI: 1.471–2.187, P<0.001; III: HR =2.905; 95% CI: 2.318–3.640, P<0.001; IV: HR =3.128; 95% CI: 2.159–4.533, P<0.001), and stage (localized: HR =0.236; 95% CI: 0.194–0.287, P<0.001; regional: HR =0.425; 95% CI: 0.362–0.499, P<0.001) were identified as independent predictors of survival. And after adjusting for known factors (age, sex, race, tumor size, marital status, histologic type, grade, stage), chemotherapy (HR =1.220; 95% CI: 1.050–1.417, P=0.009) was revealed to be an independent indicator of poor prognosis.ConclusionsThere was an increasing trend in the incidence of appendiceal cancer in the United States between 2004 and 2015. Chemotherapy was revealed to be an independent indicator of poor prognosis, which provide valuable insight into the therapy of primary appendiceal cancer. Large clinical trials of chemotherapy and targeted therapy for appendiceal cancer are urgently needed. 相似文献
86.
目的探讨不同海拔低氧环境对移居青少年无氧代谢阈值的影响。方法使用Jae-ger自行车功量仪,心阻抗仪,用逐级运动负荷法分别测定移居海拔2 260 m、3 417 m和4 280 m三个海拔高度,13~16岁青少年的无氧代谢阈值(AT),同步测定动脉血氧饱和度(SaO2)和心输出量(CO),分析其间的相互关系。结果AT值随海拔高度的上升而下降,以氧耗量表示,分别为(27.44±6.50)、(22.88±5.38)和(16.95±5.97)m l/m in.kg。AT值的降低与SaO2和CO的降低呈正相关。结论SaO2和CO是影响AT值的重要因素。长期移居高海拔的青少年AT值降低,海拔越高,降低越明显。 相似文献
87.
88.
UPLC(超高效液相色谱)/MS/MS联用技术测定全血中的西罗莫司 总被引:2,自引:0,他引:2
目的 建立全血中西罗莫司的UPLC(超高效液相色谱)/MS/MS测定方法,方法 全血样品中加入他克莫司(FK506)作内标,用叔丁基甲醚进行提取。以乙腈与含千分之五甲酸的水溶液为流动相(40:50),色谱柱为AcquityUPLCTMBEHC。(50mm×2.1mm,1.7μm),流速0.5ml/min。三重四极杆质谱采用正离子模式,离子采集方式为多反应监测模式(MRM),离子源温度105℃,离子源电离电压为3300V,雾化气流速500L/h。采集离子(母离子/子离子)西罗莫司为931.2/864.1,FK506为822.0/577.0。结果 西罗莫司在1~240腿/L浓度范围内呈良好的线性(n=0.9995)。日内、日间精密度均在15%以内,提取回收率大于75.0%,方法回收率为95.0%~98.5%,最低检测限为0.2μg/L。结论 本方法灵敏、准确,适合临床西罗莫司的全血分析。 相似文献
89.
90.
奥美拉唑不同途径、不同剂量给药抑制胃酸的效果 总被引:12,自引:4,他引:12
目的 :研究不同途径、不同剂量奥美拉唑抑制胃酸的效果 ,指导临床合理用药。方法 :将 10 0例内窥镜证实为活动期消化性溃疡病人分成A组 4 0例 ,静脉推注奥美拉唑 4 0mg ,bid ;B组 36例 ,口服奥美拉唑 2 0mg ,bid ;C组 2 4例 ,口服奥美拉唑 2 0mg ,qd。监测用药后d 3~ 5胃内 2 4hpH值变化 ,对A ,B 2组中各 10例 (为A2 ,B2 组 )测定了首次给药后的药物起效时间。结果 :A ,B组的抑酸效果相仿 (P >0 .0 5) ,胃内 pH值分别为 7.0 7±s0 .15和6 .9± 0 .6 ,与C组胃内 pH值 5.8± 1.4相比 ,差异有非常显著意义 (P <0 .0 1) ;A2 组起效时间为 (0 .7± 0 .6 )h ,B2 组为 (4.8± 1.0 )h ,差异有非常显著意义 (P <0 .0 1)。结论 :不同途径 3种剂量的奥美拉唑均有良好的抑酸效果 ,A ,B组更佳 ;静脉用药起效更迅速 相似文献